Potential prognostic biomarkers of hepatocellular carcinoma based on 4D label-free quantitative proteomics analysis pilot investigation.

BACKGROUND: Hepatocellular carcinoma carries a poor prognosis and poses a serious threat to global health. Currently, there are few potential prognostic biomarkers available for the prognosis of hepatocellular carcinoma. METHODS: This pilot study used 4D label-free quantitative proteomics to compare the proteomes of hepatocellular carcinoma and adjacent non-tumor tissue. A total of 66,075 peptides, 6363 identified proteins, and 772 differentially expressed proteins were identified in specimens from three hepatocellular carcinoma patients. Through functional enrichment analysis of differentially expressed proteins by Gene Ontology, KEGG pathway, and protein domain, we identified proteins with similar functions. RESULTS: Twelve differentially expressed proteins (RPL17, RPL27, RPL27A, RPS5, RPS16, RSL1D1, DDX18, RRP12, TARS2, YARS2, MARS2, and NARS1) were selected for identification and validation by parallel reaction monitoring. Subsequent Western blotting confirmed overexpression of RPL27, RPS16, and TARS2 in hepatocellular carcinoma compared to non-tumor tissue in 16 pairs of clinical samples. Analysis of The Cancer Genome Atlas datasets associated the increased expression of these proteins with poor prognosis. Tissue microarray revealed a negative association between high expression of RPL27 and TARS2 and the prognosis of hepatocellular carcinoma patients, although RPS16 was not significant. CONCLUSIONS: These data suggest that RPL27 and TARS2 play an important role in hepatocellular carcinoma progression and may be potential prognostic biomarkers of overall survival in hepatocellular carcinoma patients.

The potential applications of artificially modified exosomes derived from mesenchymal stem cells in tumor therapy.

Mesenchymal stem cells (MSCs) have tumor-homing ability and play critical roles in tumor treatment, but their dual influences on tumor progression limit their therapeutic applications. Exosomes derived from MSCs (MSC-exosomes) exhibit great potential in targeted tumor treatment due to their advantages of high stability, low immunogenicity, good biocompatibility, long circulation time and homing characteristics. Furthermore, the artificial modification of MSC-exosomes could amplify their advantages and their inhibitory effect on tumors and could overcome the limit of tumor-promoting effect. In this review, we summarize the latest therapeutic strategies involving artificially modified MSC-exosomes in tumor treatment, including employing these exosomes as nanomaterials to carry noncoding RNAs or their inhibitors and anticancer drugs, and genetic engineering modification of MSC-exosomes. We also discuss the feasibility of utilizing artificially modified MSC-exosomes as an emerging cell-free method for tumor treatment and related challenges.

Two cases of variations of musculocutaneous nerve communication with the median nerve / Dos casos de variaciones de la comunicación del nervio musculocutáneo con el nervio mediano

SUMMARY: To know the nerve variations of brachial plexus and its branches is very important in the management of upper limb nerve injuries. Variations of the brachial plexus are not uncommon, but types of variations are diverse. The unusual communication branches between the musculocutaneous nerve (MCN) and the median nerve (MN) in course were found during routine dissection on the two different left arms of formalin fixed male cadavers. Depending on the position related to the coracobrachial muscle (CBM), one MCN pierced the CBM, the other did not in the two cases. The branches of MCN emerged interior to the coracoid process to innervate the CBM. The present case reports of anatomical variations of nerves can help to manage nerve injuries and plan surgical approaches during surgical procedures.

RESUMEN: Conocer las variaciones nerviosas del plexo braquial y sus ramas es muy importante en el tratamiento de las lesiones nerviosas de los miembros superiores. Las variaciones del plexo braquial no son infrecuentes, sin embargo los tipos de variaciones son diversos. Los ramos inusuales de comunicación entre el nervio musculocutáneo (NMC) y el nervio mediano (NM) en curso fueron descubiertos durante la disección de rutina en dos miembros superiores izquierdos de dos cadáveres de sexo masculino fijados con formalina. Un NMC atravesó el MCB, otro no lo hizo en los dos casos. Los ramos de NMC emergieron a nivel del proceso coracoideo para inervar el MCB. Los presentes informes de casos de variaciones anatómicas de los nervios pueden ayudar a tratar las lesiones nerviosas y planificar los abordajes quirúrgicos durante los procedimientos quirúrgicos.