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1.
Biol Trace Elem Res ; 177(1): 10-15, 2017 May.
Artículo en Inglés | MEDLINE | ID: mdl-27726061

RESUMEN

The aim of this study was to investigate the effects of copper ions on decidualization of human endometrial stromal cells (HESCs) cultured in vitro. Firstly, non-toxic concentrations of copper D-gluconate were screened in HESCs based on cell activity. Then, the effects of non-toxic concentrations of copper ions (0~250 µM) were examined on decidualization of human endometrial stromal cells. Our data demonstrated that the mRNA expressions of insulin-like growth factor binding protein (IGFBP-1), prolactin (PRL), Mn-SOD, and FOXO1were down-regulated during decidualization following the treatments with 100 or 250 µM copper ions. Meanwhile, the amount of malonaldehyde (MDA) in the supernatant of HESCs was increased. These results showed that in vitro decidualization of HESCs was impaired by copper treatment.


Asunto(s)
Cobre/farmacología , Células del Estroma/efectos de los fármacos , Cobre/administración & dosificación , Relación Dosis-Respuesta a Droga , Humanos , Iones/administración & dosificación , Iones/farmacología , Células del Estroma/metabolismo
2.
Biol Trace Elem Res ; 173(2): 427-32, 2016 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-27025717

RESUMEN

The molecular mechanism for copper toxicity on spermatozoa quality in mice is not well understood. In a 4-week experiment, we challenged 24, 6-week-old male CD-1 mice with twice-a-week intraperitoneal copper chloride injections and evaluated spermatozoa quality, copper levels in the testes, serum testosterone, the expression of key antioxidant glutathione peroxidase 5 (GPx5), and the regulated androgen receptor (AR) in the mice testes. We compared these outcomes for four groups of six mice given doses of 0, 1.25, 2.5, 5.0 mg/kg weight copper chloride twice a week for 4 weeks. The mice demonstrated a copper increase spermatozoa head malformation in a dose-response manner. However, we observed no changes in spermatozoa viability and acrosome integrity in the ratio of mouse body weight to testes weight or in the histomorphology of the testes as the average copper level increased. Results of our RT-PCR assays, immunohistochemical tests, ELISA, and histochemistry analyses indicated that testis GPx5 expression was increased, AR expression in the testes was decreased, serum testosterone was decreased, and the activity of 3ß-hydroxysteroid dehydrogenase was decreased as the copper dose increased. In conclusion, these data show that sublethal exposure to copper induces spermatozoa head malformation and influences both mRNA and protein levels of GPx5 and AR which is related to copper resides in the testes.


Asunto(s)
Acrosoma/metabolismo , Cobre/toxicidad , Estrés Oxidativo/efectos de los fármacos , Testículo/metabolismo , Acrosoma/patología , Animales , Glutatión Peroxidasa/biosíntesis , Masculino , Ratones , Receptores Androgénicos/biosíntesis , Testículo/patología
3.
Nan Fang Yi Ke Da Xue Xue Bao ; 28(7): 1136-40, 2008 Jul.
Artículo en Chino | MEDLINE | ID: mdl-18676245

RESUMEN

OBJECTIVE: To evaluate the effect of human hair keratin (HHK) in peripheral nerve repair and explore the mechanism of sciatic nerve regeneration. METHODS: Rat models of sciatic nerve damage was established by creating a 10-mm gap in the sciatic nerve, which was bridged with a HHK implant. Histological examinations of the nerve tissues were performed at different time points after the surgery. RESULTS: During the period from 2 days to 2 weeks following HHK implantation, Schwann cells were found to undergo dedifferentiation and proliferate along the HHK implant. Three weeks after HHK implantation, numerous macrophages and megakaryocytes occurred around the HHK, and a large quantity of regenerated Schwann cells aligned in orderly fashion was seen between the fine filaments of partially degraded HHK, where axons and capillaries were also observed. Six weeks later, massive nerve fibers and capillaries developed around the HHK, and at 9 weeks, the HHK implant was substantially degraded and numerous regenerated nerve fibers occurred characterized by obvious epineurium and perineurium. Till 12 weeks after HHK implantation, HHK was almost completely degraded and replaced by the newly regenerated nerve fibers that had grown across the nerve defect. CONCLUSIONS: HHK is an ideal material for nerve injury repair. Apocytosis plays a key role in the differentiation process of highly differentiated Schwann cells into immature Schwann cells following nerve injury. As a protective mechanism, the axons undergo enclosure and dissociation following injuries, and the intact axons give rise to growth cones that extend fibers of growing buds to competitively bind the one or more Schwann cells, but only one such but finally develops into a complete axon. The nerve fiber barrier membrane is derived from the capillary menchymal stem cells and the outmost vascular barrier membrane. The regeneration of the Schwann cells, axons and the nerve membrane is the result of self-organization through a well synchronized and coordinated mechanism.


Asunto(s)
Queratinas/farmacología , Regeneración Nerviosa/efectos de los fármacos , Nervio Ciático/fisiopatología , Animales , Femenino , Cabello/química , Humanos , Queratinas/administración & dosificación , Masculino , Regeneración Nerviosa/fisiología , Prótesis e Implantes , Distribución Aleatoria , Ratas , Ratas Sprague-Dawley , Nervio Ciático/lesiones
5.
Di Yi Jun Yi Da Xue Xue Bao ; 25(9): 1128-31, 1144, 2005 Sep.
Artículo en Chino | MEDLINE | ID: mdl-16174579

RESUMEN

OBJECTIVE: To culture Schwann cells (SCs) and human hair keratins (HHKs) for artificial nerve bridge construction. METHODS: SCs were purified by primary culture and labeled with BrdU, which were then cultured with HHKs decorated by ECM. The artificial nerve bridge was implanted into the defect of sciatic nerve, beneath the skin, and in the skeletal muscles of SD rat, respectively. The morphology of the SCs cultured with HHKs was monitored by inverted microscope and evaluated by immunocytochemical staining. Growth of BrdU-labeled SCs in vivo was observed by immunocytochemical staining on paraffin sections. RESULTS: In vitro cultured SCs were capable of adhering to HHKs and grew well four weeks after implantation. The HHK component in the artificial nerve bridge underwent degradation in the defect of the sciatic nerve, beneath the skin, and in the skeletal muscles of SD rat, and SC survival and proliferation were verified. CONCLUSION: SCs can survive in three-dimensional culture with HHKs for construction of artificial nerve bridge to repair nerve defects.


Asunto(s)
Movimiento Celular/fisiología , Cabello/química , Queratinas/farmacología , Regeneración Nerviosa/fisiología , Células de Schwann/citología , Animales , Animales Recién Nacidos , Axones/fisiología , Células Cultivadas , Humanos , Tejido Nervioso , Ratas , Ratas Sprague-Dawley , Nervio Ciático/citología , Nervio Ciático/lesiones , Nervio Ciático/cirugía
6.
Di Yi Jun Yi Da Xue Xue Bao ; 24(10): 1150-2, 2004 Oct.
Artículo en Chino | MEDLINE | ID: mdl-15485787

RESUMEN

OBJECTIVE: To establish a fast and simple method for screening mutant yeast strains. MATERIALS AND METHODS: Homologous recombination technique was used to detect mutant yeast strains in yeast genomic library, with the green fluorescent protein gene as the reporter gene in the transposon. RESULTS: The strains that emitted green fluorescence were isolated, indicating that the gfp gene was inserted into the yeast genome by homologous recombination. CONCLUSION: This study established a useful method for functional genome study by homologous recombination technique, and provide an alternative for gene therapeutic drug development.


Asunto(s)
Genes Fúngicos/genética , Proteínas Fluorescentes Verdes/genética , Mutación , Recombinación Genética , Levaduras/genética , Clonación Molecular/métodos , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas Fluorescentes Verdes/análisis
7.
Di Yi Jun Yi Da Xue Xue Bao ; 23(10): 1025-7, 2003 Oct.
Artículo en Chino | MEDLINE | ID: mdl-14559683

RESUMEN

OBJECTIVE: To evaluate the relationship between receptor-mediated endocytosis, autophagosome formation and apoptosis by observing the morphological changes of mouse peritoneal macrophages in the course of receptor-mediated endocytotic activities, autophagy and apoptosis. METHODS: Mouse peritoneal macrophages were incubated with horseradish peroxidase-labeled concanavalin A (ConA-HRP), and morphological examinations were performed at different time points after the incubation. RESULTS: After the incubation of the macrophages with ConA-HRP, ConA-HRP was observed to enter the vesicles by way of receptor-mediated endocytosis. Three kinds of endosomes were observed, namely vesicles, tubes and double-membrane sheets. The double-membrane sheets enveloped a portion of the cytoplasm and organelles, thus giving rise to vesicles, or the autophagosomes, which later fused with lysosome, followed by the apoptosis of the macrophages. CONCLUSION: Receptor-mediated endocytosis of ConA-HRP is associated with autophagy and apoptosis.


Asunto(s)
Apoptosis , Autofagia , Endocitosis , Macrófagos Peritoneales/ultraestructura , Receptores de Superficie Celular/fisiología , Animales , Femenino , Masculino , Ratones , Microscopía Electrónica de Rastreo
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