The photosynthetic toxicity of nano-polystyrene to Microcystis aeruginosa is influenced by surface modification and light intensity.

It is known that nanoplastics can cause membrane damage and production of reactive oxygen species (ROS) in cyanobacteria, negatively impacting their photosynthetic reactions and growth. However, the synergistic effect of light intensity on nanoplastics' toxicity to cyanobacteria is rarely investigated. Here, we investigated the impact of nano-polystyrene particles (PS) and amino-modified nano-polystyrene particles (PS-NH2) on cyanobacterium Microcystis aeruginosa cultivated under two light intensities. We discovered that PS-NH2 was more toxic to M. aeruginosa compared to PS with more damage of cell membranes by PS-NH2. The membrane damage was found by scanning electron microscope and atomic force microscopy. Under low light, PS-NH2 inhibited the photosynthesis of M. aeruginosa by decreasing the PSII quantum yield, photosynthetic electron transport rate and pigment content, but increasing non-photochemical quenching and Car/chl a ratio to cope with this stress condition. Moreover, high light appeared to increase the toxicity of PS-NH2 to M. aeruginosa by increasing its in vitro and intracellular ROS content. Specifically, on the one hand, high visible light (without UV) and PS-NH2 induced more in vitro singlet oxygen, hydroxyl radical and superoxide anion measured by electron paramagnetic resonance spectrometer in vitro, which could be another new toxic mechanism of PS-NH2 to M. aeruginosa. On the other hand, high light and PS-NH2 might increase intracellular ROS by inhibiting more photosynthetic electron transfer and accumulating more excess energy and electrons in M. aeruginosa. This research broadens our comprehension of the toxicity mechanisms of nanoplastics to cyanobacteria under varied light conditions and suggests a new toxic mechanism of nanoplastics involving in vitro ROS under visible light, providing vital information for assessing ecotoxicological effects of nanoplastics in the freshwater ecosystem.

Light modulates the effect of antibiotic norfloxacin on photosynthetic processes of Microcystis aeruginosa.

Norfloxacin is one of the widely used antibiotics, often detected in aquatic ecosystems, and difficultly degraded in the environment. However, how norfloxacin affects the photosynthetic process of freshwater phytoplankton is still largely unknown, especially under varied light conditions. In this study, we investigated photosynthetic mechanisms of Microcystis aeruginosa in responses to antibiotic norfloxacin (0-50 µg/L) for 72 h under low (LL; 50 µmol photons m-2 s-1) and high (HL; 250 µmol photons m-2 s-1) growth light regimes. We found that environmentally related concentrations of norfloxacin inhibited the growth rate and operational quantum yield of photosynthesis system II (PSII) of M. aeruginosa more under HL than under LL, suggesting HL increased the toxicity of norfloxacin to M. aeruginosa. Further analyses showed that norfloxacin deactivated PSII reaction centers under both growth light regimes with increased minimal fluorescence yields only under HL, suggesting that norfloxacin not only damaged reaction centers of PSII, but also inhibited energy transfer among phycobilisomes in M. aeruginosa under HL. However, non-photosynthetic quenching decreased in the studied species by norfloxacin exposure under both growth light regimes, suggesting that excess energy might not be efficiently dissipated as heat. Also, we found that reactive oxygen species (ROS) content increased under norfloxacin treatments with a higher ROS content under HL compared to LL. In addition, HL increased the absorption of norfloxacin by M. aeruginosa, which could partly explain the high sensitivity to norfloxacin of M. aeruginosa under HL. This study firstly reports that light can strongly affect the toxicity of norfloxacin to M. aeruginosa, and has vitally important implications for assessing the toxicity of norfloxacin to aquatic microorganisms.