Direct and indirect monitoring methods for nitrous oxide emissions in full-scale wastewater treatment plants: A critical review.

Mitigation of nitrous oxide (N2O) emissions in full-scale wastewater treatment plant (WWTP) has become an irreversible trend to adapt the climate change. Monitoring of N2O emissions plays a fundamental role in understanding and mitigating N2O emissions. This paper provides a comprehensive review of direct and indirect N2O monitoring methods. The techniques, strengths, limitations, and applicable scenarios of various methods are discussed. We conclude that the floating chamber technique is suitable for capturing and interpreting the spatiotemporal variability of real-time N2O emissions, due to its long-term in-situ monitoring capability and high data acquisition frequency. The monitoring duration, location, and frequency should be emphasized to guarantee the accuracy and comparability of acquired data. Calculation by default emission factors (EFs) is efficient when there is a need for ambiguous historical N2O emission accounts of national-scale or regional-scale WWTPs. Using process-specific EFs is beneficial in promoting mitigation pathways that are primarily focused on low-emission process upgrades. Machine learning models exhibit exemplary performance in the prediction of N2O emissions. Integrating mechanistic models with machine learning models can improve their explanatory power and sharpen their predictive precision. The implementation of the synergy of nutrient removal and N2O mitigation strategies necessitates the calibration and validation of multi-path mechanistic models, supported by long-term continuous direct monitoring campaigns.

Sources, colloidal characteristics, and separation technologies for highly hazardous waste nanoemulsions.

Nanoemulsions play a crucial role in various industries. However, their application often results in hazardous waste, posing significant risks to human health and the environment. Effective management and separation of waste nanoemulsions requires special attention and effort. This review provides a comprehensive understanding of waste nanoemulsions, covering their sources, characteristics, and suitable treatment technologies, intending to mitigate their environmental impact. This study examines the evolution of nanoemulsions from beneficial products to hazardous wastes, provides an overview of the production processes, fate, and hazards of waste nanoemulsions, and highlights the critical characteristics that affect their stability. The latest advancements in separating waste nanoemulsions for recovering oil and reusable water resources are also presented, providing a comprehensive comparison and evaluation of the current treatment techniques. This review addresses the significant challenges in nanoemulsion treatment, provides insights into future research directions, and offers valuable implications for the development of more effective strategies to mitigate the hazards associated with waste nanoemulsions.

Vital role of CYP450 in the biodegradation of antidiabetic drugs in the aerobic activated sludge system and the mechanisms.

The extensive use of antidiabetic drugs (ADDs) and their detection in high concentrations in the environment have been extensively documented. However, the mechanism of ADDs dissipation in aquatic environments is still not well understood. This study thoroughly investigates the dissipation behavior of ADDs and the underlying mechanisms in the aerobic activated sludge system. The results indicate that the removal efficiencies of ADDs range from 3.98% to 100% within 48 h, largely due to the biodegradation process. Additionally, the gene expression of cytochrome P450 (CYP450) is shown to be significantly upregulated in most ADDs-polluted samples (P < 0.05), indicating the vital role of CYP450 enzymes in the biodegradation of ADDs. Enzyme inhibition experiments validated this hypothesis. Moreover, molecular docking and simulation results indicate that a strong correlation between the biodegradation of ADDs and the interactions between ADDs and CYP450 (Ebinding). The differences in dissipation behavior among the tested ADDs are possibly due to their electrophilic characteristics. Overall, this study makes the initial contribution to a more profound comprehension of the crucial function of CYP450 enzymes in the dissipation behavior of ADDs in a typical aquatic environment.

Application of a water-energy-carbon coupling index to evaluate the long-term operational stability of the anaerobic-anoxic-oxic-membrane bioreactor (A2/O-MBR) process under the influence of rainstorms.

In the context of global climate change, sudden rainstorms and typhoons induce fluctuations in hydraulic shocks to wastewater treatment plants (WWTPs) in coastal areas, causing two challenges of stable effluent quality and low-carbon operation. We established a quantitative evaluation method for resistance of wastewater treatment processes to hydraulic shocks based on the water-energy-carbon nexus using operational data from a WWTP in southeast coastal China from July 2018 to December 2022. The effects of hydraulic shocks on the operational stability of the anaerobic-anoxic-oxic-membrane bioreactor (A2/O-MBR) process were analyzed following five steps. The results showed that the gray water footprint (GWF) of the process was 9.3% lower than that of the A2/O process. The energy footprint (ENF) and carbon footprint (CF) were approximately 2.1 times and 1.7 times higher than those of the A2/O process, respectively. The resistance to hydraulic shocks of the A2/O-MBR process is approximately 5.5 times higher than that of the A2/O process. In conclusion, the A2/O-MBR process exhibits higher process operational stability when subjected to hydraulic shocks, which is more conducive to the efficient and stable operation of WWTPs in rainstorm and typhoon-prone areas. The evaluation methodology provides qualitative technical support for selecting upgrading processes for WWTPs in different regions.

Mechanisms of benzene and benzo[a]pyrene biodegradation in the individually and mixed contaminated soils.

There is a lack of knowledge on the biodegradation mechanisms of benzene and benzo [a]pyrene (BaP), representative compounds of polycyclic aromatic hydrocarbons (PAHs), and benzene, toluene, ethylbenzene, and xylene (BTEX), under individually and mixed contaminated soils. Therefore, a set of microcosm experiments were conducted to explore the influence of benzene and BaP on biodegradation under individual and mixed contaminated condition, and their subsequent influence on native microbial consortium. The results revealed that the total mass loss of benzene was 56.0% under benzene and BaP mixed contamination, which was less than that of individual benzene contamination (78.3%). On the other hand, the mass loss of BaP was slightly boosted to 17.6% under the condition of benzene mixed contamination with BaP from that of individual BaP contamination (14.4%). The significant differences between the microbial and biocide treatments for both benzene and BaP removal demonstrated that microbial degradation played a crucial role in the mass loss for both contaminants. In addition, the microbial analyses revealed that the contamination of benzene played a major role in the fluctuations of microbial compositions under co-contaminated conditions. Rhodococcus, Nocardioides, Gailla, and norank_c_Gitt-GS-136 performed a major role in benzene biodegradation under individual and mixed contaminated conditions while Rhodococcus, Noviherbaspirillum, and Phenylobacterium were highly involved in BaP biodegradation. Moreover, binary benzene and BaP contamination highly reduced the Rhodococcus abundance, indicating the toxic influence of co-contamination on the functional key genus. Enzymatic activities revealed that catalase, lipase, and dehydrogenase activities proliferated while polyphenol oxidase was reduced with contamination compared to the control treatment. These results provided the fundamental information to facilitate the development of more efficient bioremediation strategies, which can be tailored to specific remediation of different contamination scenarios.

Carbon emissions of urban rail transit in Chinese cities: A comprehensive analysis.

Thoroughly exploring carbon emissions within Urban Rail Transit (URT) systems is crucial for effectively reducing emissions while satisfying increasing energy demands. This study evaluated the spatiotemporal characteristics of carbon emissions in China's URT sector. Tapio decoupling and the Logarithmic Mean Divisia Index, used to scrutinize decoupling states and identify principal contributing factors, respectively, revealed the following: (1) Total emissions increased by 217 %, with significant spatiotemporal heterogeneity from 2015 to 2022. Type I and Type II cities accounted for >85 % of emissions but exhibited lower carbon intensity. (2) Most URT cities showed expansion-negative decoupling between economic growth and carbon emissions. Developed regions show strong decoupling, and the overall decoupling status improved in 2021-2022. (3) Emissions growth was influenced by energy intensity and economic activity, and transportation intensity was the main inhibitor for Type I cities and a driving force for other cities. Finally, recommendations for carbon emission reduction in the URT industry are proposed.

Effects of short and long-term thermal exposure on microbial compositions in soils contaminated with mixed benzene and benzo[a]pyrene: A short communication.

Polycyclic aromatic hydrocarbons (PAHs) and benzene, toluene, ethylbenzene, and xylene (BTEX) are the most persistent and toxic organic contaminants often found co-contaminated in anthropogenic and petrochemical industrial sites. Therefore, an experiment was performed for the safe biodegradation of benzene and benzo[a]pyrene (BaP) through thermally-enhanced biodegradation, and to explore the influence of elevated thermal treatments on microbial diversity and composition. The results revealed that elevated thermal treatments (15 to 45 °C) significantly enhanced the diversity of both bacteria and fungi. The composition analysis revealed that short-term and long-term elevated temperature conditions can directly enhance the specificity of microorganisms that play a crucial role in the biodegradation of benzene and BaP co-contaminated soil. Moreover, the indirect role of elevated temperature conditions on microbial compositions was through the fluctuations of soil properties, especially soil pH, moisture, TOC, potassium, phosphorous, total Fe, Fe(II), and Fe(III). In addition, the correlation analyses revealed that thermal exposure enhances the synergistic association (fungal-fungal, fungal-bacterial, bacterial-bacterial) of microbes to degrade the toxic contaminants and to cope with harsh environmental conditions. These results concluded that the biodegradation of benzene and BaP co-contamination was efficiently enhanced under the thermally-enhanced biodegradation approach and the elevation of temperature can affect the microbial compositions directly via microbial specificity or indirectly by influencing the soil properties.

Responses of antibiotic resistance genes in the enhanced biological phosphorus removal system under various antibiotics: Mechanisms and implications.

The effects of antibiotics on the proliferation of antibiotic resistant genes (ARGs) in WWTPs have drawn great attention in recent years. The effects of antibiotics on ARGs in the enhanced biological phosphorus removal (EBPR) system and its mechanisms, however, are still not well understood. In this study, EBPR systems were constructed using activated sludge to investigate the effects of ten commonly detected antibiotics in the environment on the proliferation of ARGs and the mechanisms involved. The results showed that the total abundance of ARGs increased to varying degrees with the addition of different antibiotics (0.05 mmol/L), and the top 30 ARGs increased by 271.1 % to 370.0 %. Mobile genetic elements (MGEs), functional modules, and the bacteria community were consistently related to the changes in ARGs. Refractory antibiotics, in particular, have a stronger promoting effect on transduction in the EBPR system. The insertion sequence common region (ISCR) and transposon (Tnp) were identified as crucial factors in the proliferation of ARGs. Moreover, the risk of polyphosphate accumulating organisms (PAOs) carrying ARGs in the presence of antibiotics should not be ignored. Our findings emphasize the potential efficacy of employing strategies that target the reduction of MGEs, regulation of cellular communication, and management of microbial communities to effectively mitigate the risks associated with ARGs.

Insights into greenhouse gas emissions from a wastewater treatment plant in vulnerable water areas of China.

Wastewater treatment plants (WWTPs) are a significant anthropogenic source of greenhouse gas (GHG), but the quantitative assessment of GHG emissions from WWTPs in vulnerable water areas under stricter discharge limits remains unclear. Herein, depending on a case WWTP in southern China, we investigated the impacts of discharge standard improvement and key drivers of GHG emissions using daily operating data. We demonstrated that the stricter discharge limits increased the total GHG emission intensity by 18.2 %, with direct emissions increasing more than indirect GHG emissions. The GHG emissions were negatively correlated with water quantity, showing the scale effect, which became more pronounced after the discharge standard improvement. Increasing influent chemical oxygen demand and total nitrogen concentrations significantly drove the variations in GHG emissions, which were accelerated under stricter discharge limits. This study provides insights into the evaluation of GHG emission from WWTPs in vulnerable water areas and carbon-neutral wastewater management policies.

A comprehensive review on the behavior and evolution of oil droplets during oil/water separation by membranes.

Membrane separation technology has significant advantages for treating oil-in-water emulsions. Understanding the evolution of oil droplets could reveal the interfacial and colloidal interactions, facilitate the design of advanced membranes, and improve the separation performances. This review on the characteristic behavior and evolution of oil droplets focuses on the advanced analytical techniques, and the subsequent fouling as well as demulsification effects during membrane separation. A detailed introduction is provided on microscopic observations and numerical simulations of the dynamic evolution of oil droplets, featuring real-time in-situ visualization and accurate reconstruction, respectively. Characteristic behaviors of these oil droplets include attachment, pinning, wetting, spreading, blockage, intrusion, coalescence, and detachment, which have been quantified by specific proposed parameters and criteria. The fouling process can be evaluated using Hermia and resistance models. The related adhesion force and intrusion pressure as well as droplet-droplet/membrane interfacial interactions can be accurately quantified using various force analysis methods and advanced force measurement techniques. It is encouraging to note that oil coalescence has been achieved through various effects such as electrostatic interactions, mechanical actions, Laplace pressure/surface free energy gradients, and synergistic effects on functional membranes. When oil droplets become destabilized and coalesce into larger ones, the functional membranes can overcome the limitations of size-sieving effect to attain higher separation efficiency. This not only bypasses the trade-off between permeability and rejection, but also significantly reduces membrane fouling. Finally, the challenges and potential research directions in membrane separation are proposed. We hope this review will support the engineering of advanced materials for oil/water separation and research on interface science in general.

Applications of functional nanoparticle-stabilized surfactant foam in petroleum-contaminated soil remediation.

Surfactant foam (SF) can be used to remediate petroleum-contaminated soil because of its easy transfer to inhomogeneous and low-permeability formations. Nanoparticles (NPs) not only stabilize SF under extreme conditions but also impart various functions, aiding the removal of petroleum contaminants. This review discusses the stabilization mechanisms of nanoparticle-stabilized SF (NP-SF) as well as the effects of NP size, chargeability, wettability, and NP-to-surfactant ratio on foam stability. SF stabilized by inert SiO2 NPs is most commonly used to remediate soil contaminated with crude oil and diesel. Low dose of SF stabilized by nano zero-valent iron is cost-effective for treating soil contaminated with chlorinated organics and heavy metal ions. The efficiency and recyclability of Al2O3/Fe3O4 NPs in the remediation of diesel and crude oil contamination could be enhanced by applying a magnetic field. This review provides a theoretical basis and practical guidelines for developing functional NP-SF to improve the remediation of petroleum-contaminated soils. Future research should focus on the structural design of photocatalytic NPs and the application of catalytic NP-SF in soil remediation.

Differential variations of intracellular and extracellular antibiotic resistance genes between treatment units in centralized sewage sludge treatment plants.

Centralized sludge treatment plants (CSTPs) are implicated as strong hotspots of antibiotic resistance genes (ARGs). However, the knowledge gap on the fate of intracellular and extracellular ARGs (iARGs and eARGs), and the functionality of resistant hosts limit risk assessment and management of CSTP resistome. Here, the flow of iARGs and eARGs across treatment units and analyses of ARG hosts were systematically explored in three full-scale CSTPs using quantitative metagenomic approaches. We found that 29% of sludge ARGs could be removed, with iARGs being dominant in the produced biosolids. The treatment process significantly affected the variations of iARG and eARG abundance while no significant difference in composition between iARGs and eARGs was observed in CSTPs. 15% of 295 recovered genomes were identified as antibiotic-resistant hosts, among which Actinobacteriota tended to encode multiple resistance. The key functions of ARG hosts were relative to the biological organic removal (e.g., carbohydrates). There also existed relationships between certain resistance mechanisms and functional traits, indicating that ARGs might take part in the physiological process of microorganisms in the sludge treatment. These findings provide important insight into the differential resistome variations and host functionality, which would be crucial in the management of antibiotic resistance in CSTPs.

Combination of biochar and AMF promotes phosphorus utilization by stimulating rhizosphere microbial co-occurrence networks and lipid metabolites of Phragmites.

Agricultural biochar and arbuscular mycorrhizal fungi were used to promote the growth of Phragmites in the structural damaged and nutritional imbalanced littoral zone soils. Wheat straw biochar played a significant role in improving soil porosity and supplementing available phosphorus to 79.20 ± 3.20 mg/kg, compared with CK at 17.50 ± 0.88 mg/kg. The addition of Diversispora versiformis improved the plant net photosynthetic rate reaching up to 25.66 ± 0.65 µmol·m-2·s-1, which was 36.60 % higher than CK. The combination of biochar and fungi contributed to the whole plant dry weight biomass of 32.30 % and 234.00 % higher than the single biochar or AMF amendment groups, respectively. Meanwhile, the analysis of microbial co-occurrence networks showed the most relevant networks node species were mainly Talaromyces, Chaetomiacea and Gemmatimonadetes etc. Root lipid metabolite of Glycerophospholines further proved that phosphorus utilization was also enhanced endogenously in the rhizosphere soil. These results indicate that the combination of biochar and arbuscular mycorrhizal fungi play synergic role in enhancing phosphorus utilization endogenously and exogenously.

Coupling surfactants with ISCO for remediating of NAPLs: Recent progress and application challenges.

Non-aqueous phase liquids (NAPLs) pose a serious risk to the soil-groundwater environment. Coupling surfactants with in situ chemical oxidation (ISCO) technology is a promising strategy, which is attributed to the enhanced desorption and solubilization efficiency of NAPL contaminants. However, the complex interactions among surfactants, oxidation systems, and NAPL contaminants have not been fully revealed. This review provides a comprehensive overview on the development of surfactant-coupled ISCO technology focusing on the effects of surfactants on oxidation systems and NAPLs degradation behavior. Specifically, we discussed the compatibility between surfactants and oxidation systems, including the non-productive consumption of oxidants by surfactants, the role of surfactants in catalytic oxidation systems, and the loss of surfactants solubilization capacity during oxidation process. The effect of surfactants on the degradation behavior of NAPL contaminants is then thoroughly summarized in terms of degradation kinetics, byproducts and degradation mechanisms. This review demonstrates that it is crucial to minimize the negative effects of surfactants on NAPL contaminants oxidation process by fully understanding the interaction between surfactants and oxidation systems, which would promote the successful implementation of surfactant-coupled ISCO technology in remediation of NAPLs-contaminated sites.

Destructing surfactant network in nanoemulsions by positively charged magnetic nanorods to enhance oil-water separation.

The separation of ultrafine oil droplets from wasted nanoemulsions stabilized with high concentration of surfactants is precondition for oil reuse and the safe discharge of effluent. However, the double barriers of the interfacial film and network structures formed by surfactants in nanoemulsions significantly impede the oil-water separation. To destroy these surfactant protective layers, we proposed a newly-developed polyethyleneimine micelle template approach to achieve simultaneous surface charge manipulation and morphology transformation of magnetic nanospheres to magnetic nanorods. The results revealed that positively charged magnetic nanospheres exhibited limited separation performance of nanoemulsions, with a maximum chemical oxygen demand (COD) removal of 50%, whereas magnetic nanorods achieved more than 95% COD removal in less than 30 s. The magnetic nanorods were also applicable to wasted nanoemulsions from different sources and exhibited excellent resistance to wide pH changes. Owing to their unique one-dimensional structure, the interfacial dispersion of magnetic nanorods was significantly promoted, leading to the efficient capture of surfactants and widespread destruction of both the interfacial film and network structure, which facilitated droplet merging into the oil phase. The easy-to-prepare and easy-to-tune strategy in this study paves a feasible avenue to simultaneously tailor surface charge and morphology of magnetic nanoparticles, and reveals the huge potential of morphology manipulation for producing high-performance nanomaterials to be applied in complex interfacial interaction process. We believe that the newly-developed magnetic-nanorods significantly contribute to hazardous oily waste remediation and advances technology evolution toward problematic oil-pollution control.

The combined effect of Diversispora versiformis and sodium bentonite contributes on the colonization of Phragmites in cadmium-contaminated soil.

To promote the colonization of Phragmites in Cd polluted, nutrient deprived and structural damaged soil, the combined remediation using chemical and microbial modifiers were carried out in potting experiments. The co-application of Diversispora versiformis and sodium bentonite significantly improved the soil structure and phosphorus utilization of the plant, while decreasing the content of cadmium bound by diethylenetriaminepentaacetic acid by 77.72%. As a result, the Phragmites height, tillers, and photosynthetic capacity were increased by 71.60%, 38.37%, and 17.54%, respectively. Further analysis suggested the co-application increased the abundance of phosphorus-releasing microbial communities like Pseudomonassp. and Gemmatimonadetes. Results of rhizosphere metabolites also proved that the signal molecule of lysophosphatidylcholine regulated the phosphorus fixation and utilization by the plant. This work finds composite modifiers are effective in the colonization of Phragmites in Cd contaminated soil by decreasing the bioavailable Cd, increasing the abundance of functional microbial communities and regulating the phosphorus fixation.

Coupling magnetic particles with flocculants to enhance demulsification and separation of waste cutting emulsion for engineering applications.

Magnetic particles were coupled with a flocculant to enhance the demulsification and separation of waste cutting emulsions. The optimal magnetic particle size and critical magnetic field conditions were investigated to achieve large-scale engineering application of magnetic demulsification separation for waste cutting emulsion treatment. The micro-scale magnetic particles were found to show comparable effects to nano-scale magnetic particles on enhancing the demulsification and separation of cutting emulsions, which are beneficial for broadening the selectivity of low-cost magnetic particles. The critical magnetic separation region was determined to be an area 40 mm from the magnetic field source. Compared to the flocculant demulsification, the magnetic demulsification separation exhibited a significant advantage in accelerating flocs-water separation by decreasing the separation time of flocs from 180-240 min to less than 15 min, compressing the flocs by reducing the floc volume ratio from 60%-90% to lower than 20%, and showing excellent adaptability to the variable properties of waste cutting emulsions. Coupled with the design of the magnetic disk separator, continuous demulsification separation of the waste cutting emulsion was achieved at 1.0 t/hr for at least 10 hr to obtain clear effluent with 81% chemical oxygen demand removal and 89% turbidity reduction. This study demonstrates the feasibility of applying magnetic demulsification separation to large-scale continuous treatment of waste emulsion. Moreover, it addresses the flocs-water separation problems that occur in practical flocculant demulsification engineering applications.

Research progress in solid carbon source-based denitrification technologies for different target water bodies.

Nitrogen pollution in water bodies is a serious environmental issue which is commonly treated by various methods such as heterotrophic denitrification. In particular, solid carbon source (SCS)-based denitrification has attracted widespread research interest due to its gradual carbon release, ease of management, and long-term operation. This paper reviews the types and properties of SCSs for different target water bodies. While both natural (wheat straw, wood chips, and fruit shells) and synthetic (polybutylene succinate, polycaprolactone, polylactic acid, and polyhydroxyalkanoates) SCSs are commonly used, it is observed that the denitrification performance of the synthetic sources is generally superior. SCSs have been used in the treatment of wastewater (including aquaculture wastewater), agricultural subsurface drainage, surface water, and groundwater; however, the key research aspects related to SCSs differ markedly based on the target waterbody. These key research aspects include nitrogen pollutant removal rate and byproduct accumulation (ordinary wastewater); water quality parameters and aquatic product yield (recirculating aquaculture systems); temperature and hydraulic retention time (agricultural subsurface drainage); the influence of dissolved oxygen (surface waters); and nitrate-nitrogen load, HRT, and carbon source dosage on denitrification rate (groundwater). It is concluded that SCS-based denitrification is a promising technique for the effective elimination of nitrate-nitrogen pollution in water bodies.

A review of the interfacial stability mechanism of aging oily sludge: Heavy components, inorganic particles, and their synergism.

Oily sludge is widely produced in the processes of petroleum exploitation, storage, transportation, and refining, and becomes more stable during aging. The interfacial stability of aging oily sludge hinders the recovery and disposal of oil resources. This review summarizes the interfacial film stability of aging oily sludge, which occurs through the formation of viscoelastic and rigid bilayer interfacial films between heavy components (asphaltenes and resins) and inorganic particles. The bilayer interfacial films enhance interfacial film strength and hinder the aggregation of droplets, contributing to the formation of a stable and high-viscosity oil-water-solid three-phase mixture. Recent demulsification technologies for reducing the stability of interfacial films have been classified as follows: removing heavy components, changing asphaltene aggregate structure, and reducing inorganic particle content. More efficient demulsification technologies are expected to be developed by deeply analyzing the microstructure and interfacial properties of asphaltenes and resins, as well as comprehensively studying the complex interactions among various components. This review constructs a bridge between the stability mechanism and the corresponding destabilization methods, which would promote future studies in aging oily sludge treatment.

Effects of antibiotics on enhanced biological phosphorus removal and its mechanisms.

Many kinds of antibiotics are continuously discharged into wastewater and typically cause a great decrease in sewage treatment performance, whereas mechanisms of differences in the impacts of commonly used antibiotics on phosphate removal are still elusive. Thus, an enhanced biological phosphorus removal (EBPR) system, as an effective method of phosphate removal, was developed, and its performance in the treatment of artificial wastewater containing antibiotics at short- (8 h) and long-term (15 days) exposure was investigated. The results show that phosphorus removal was consistently inhibited by the addition of antibiotics with a significant difference (P < 0.05). To interpret the phenomena, mechanistic equations were developed, and the results indicate that for short-term tests, the difference was mainly caused by the suppression of polyhydroxyalkanoate (PHA) degradation and the activity of polyphosphate kinase (PPK), resulting in the different inhibition of the soluble orthophosphorus (SOP) uptake process. For long-term tests, the difference in SOP uptake was principally caused by the inhibition of PHA degradation and the activity of PPK, whereas the difference in SOP release resulted from the inhibition of activities of exopolyphosphatase (PPX) and adenylate kinase (ADK). Moreover, micro-mechanisms of such inhibition were identified from molecular docking and electrostatic potential.