Emphysema is one of the pathological hallmarks of chronic obstructive pulmonary disease. We have recently reported that radiofrequency therapy improves lung function in rodent models of emphysema. However, preclinical data using large animals is necessary for clinical translation. Here, we describe the work performed to establish a unilateral porcine emphysema model. Different doses of porcine pancreatic elastase (PPE) were instilled into the left lung of 10 Yucatan pigs. Three additional pigs were used as controls. Six weeks after instillation, lungs were harvested. Lung compliance was measured by a water displacement method and plethysmography. Systematic uniform random sampling of the left and right lungs was performed independently to measure alveolar surface area using micro-computed tomography (micro-CT) and histology. In pigs instilled with 725-750 U/kg of PPE (PPE group, n = 6), the compliance of the left lung was significantly higher by 37.6% than that of the right lung (P = 0.03) using the water displacement method. With plethysmography, the volume of the left lung was significantly larger than that of the right lung at 3, 5, and 10 cmH2O. Measurements from either micro-CT or histology images showed a significant decrease in alveolar surface area by 14.2% or 14.5% (P = 0.031) in the left lung compared with the right lung of the PPE group. A unilateral model for mild emphysema in Yucatan pigs has been established, which can now be used for evaluating novel therapeutics and interventional strategies.NEW & NOTEWORTHY For clinical translation, preclinical data using large animal models is necessary. However, papers describing an emphysema model in pigs, which are anatomically and physiologically similar to humans, are lacking. Here, we report success in creating a unilateral mild-emphysema model in pigs with only one single dose of porcine pancreatic elastase. This model will be useful in bringing novel technologies and therapies from small animals to humans with emphysema.
Emphysema , Pulmonary Emphysema , Humans , Swine , Animals , Pancreatic Elastase/adverse effects , X-Ray Microtomography , Lung , Emphysema/pathology , Water , Disease Models, Animal
BACKGROUND: Sea turtle hatchlings must avoid numerous predators during dispersal from their nesting beaches to foraging grounds. Hatchlings minimise time spent in predator-dense neritic waters by swimming almost continuously for approximately the first 24 h post-emergence, termed the 'frenzy'. Post-frenzy, hatchling activity gradually declines as they swim in less predator-dense pelagic waters. It is well documented that hatchlings exhibit elevated metabolic rates during the frenzy to power their almost continuous swimming, but studies on post-frenzy MRs are sparse. RESULTS: We measured the frenzy and post-frenzy oxygen consumption of hatchlings of five species of sea turtle at different activity levels and ages to compare the ontogeny of mass-specific hatchling metabolic rates. Maximal metabolic rates were always higher than resting metabolic rates, but metabolic rates during routine swimming resembled resting metabolic rates in leatherback turtle hatchlings during the frenzy and post-frenzy, and in loggerhead hatchlings during the post-frenzy. Crawling metabolic rates did not differ among species, but green turtles had the highest metabolic rates during frenzy and post-frenzy swimming. CONCLUSIONS: Differences in metabolic rate reflect the varying dispersal stratagems of each species and have important implications for dispersal ability, yolk consumption and survival. Our results provide the foundations for links between the physiology and ecology of dispersal of sea turtles.
