[Ultrastructure of human L-68 diploid cells, infected with rubella virus]. / Ul'trastruktura diploidnykh kletok cheloveka L-68, infitsirovannykh virusom krasnukhi.

A monolayer of human diploid cell culture L-68 (21st and 30th passages) was infected with a intermediate variant of rubella vaccine strain Orlov and studied for 8 days by light and electron microscopy. Virus reproduction was associated with slight cytopathic effect; virions were detected in low amounts inside vacuoles and cisterns of the lamellar complex, groups of virions were found in the cytoplasm in association with cytomembranes and outside cells. Cell cultures differed by the cytopathic effect of the virus on the monolayer: microfocuses were found in the 21st passage culture, while in the 30th passage culture the monolayer was diffusely damaged.

[Elaboration of live measles vaccine technology on human embryo lung diploid cell culture L-68]. / Razrabotka tekhnologii proizvodstva zhivoi korevoi vaktsiny na osnove kul'tury kletok émbriona cheloveka L-68.

Measles predominates among childhood droplet infections in many countries. Immunization of all human beings sensitive to this infection is the only radical measure in controlling measles. The quality of a vaccine is primarily determined by the properties of the virus strains and cell cultures and technology of production. Now live measles vaccine is produced in or country on the basis of fibroblasts from Japanese quail embryo. The production of live measles vaccine in the primary cell cultures has a number of drawbacks caused by the nonstandard pattern of the substrate and the probability of contamination. The use the certified human diploid cells deposited in liquid nitrogen in sufficient quantities is promising. The authors have elaborated a new technology of live measles vaccine production by using the Leningrad-16 virus strain on the basis of attested L-68 diploid cell culture from the human fetal lung. Experimental batches of vaccine were obtained and attested in accordance with the present requirements for immunobiological products.

[Prospects of use human fetal fibroblasts in the treatment of various etiology wounds]. / Perspektivy ispol'zovaniia fetal'nykh fibroblastov cheloveka pri lechenii ran razlichnoi étiologii.

The investigations have demonstrated a high efficiency of the certified human fetal lung fibroblasts in the treatment of skin wounds of various etiology. For treatment, a fibroblast monolayer grown on a backing was applied to various types of wounds (burns frostbites, donor site suppurations) and in shin phlegmon. There was wound debrediment and rapid skin recovery. Employing the cells from the certified cell culture banks provides not only a biologically and genetic uniform material, but also makes it possible to standardize treatments which are useful for wide medical application, by delivering the standard cells to medical institutions which have no experience in culturing. To set up banks of cell cultures and to store them for a long time at a temperature of liquid nitrogen in adequate quantities of ampoules allow the cultured fibroblasts to be provided with if a large quantity of cells is required, which may be useful in emergencies.

[Use of collase for culturing cells]. / Primenenie kollazy dlia kul'tivirovaniia kletok.

Collase, an enzymatic preparation made from crab hepatopancreas, was used as a dissociative reagent in preparation of primary cell cultures and for detachment of continuous cells from the substrate during reinoculation. Collase was found to increase viable cell harvest by 2 to 2.5 times in comparison with trypsin and had a less detrimental effect on the cells which retained their proliferative activity, morphology, productivity, and sensitivity to viruses.

[A karyotype study of the Vero cell line cultured long term in a monolayer by static and roller methods]. / Issledovanie kariotipa kletok linii Vero, dlitel'no kul'tiviruemykh v monosloe staticheskim i rollernym sposobami.

A cytogenetic investigation of Vero cells, before and after adaptation to the medium containing a cattle serum, was carried out by methods of differential chromosome staining. Under these conditions, both the modal number of chromosomes (from 58 to 55) and the karyotype structure, namely the copy number of normal chromosomes and the marker composition were shown to change. The Vero cell karyotype stability was studied in the continued culture by the static (50 passages) and roll-bottle (37 passages) methods. The quantitative changes (the rising percentage of diploid cells, and the change of cell fraction involving the modal number of chromosomes) were shown to occur in spite of the chromosome composition stability, which limits the time of using Vero cells as a substrate for preparation of vaccines.