Development of dairy herd of transgenic goats as biofactory for large-scale production of biologically active recombinant human lactoferrin.

The primary male-goats Lac-1 (human lactoferrin gene construct hLF5) and Lac-2 (human lactoferrin gene construct hLF3) with genome containing human lactoferrin gene were bred and the sperm bank of primary male-goats and their male descendents (F1-F7) was created. The herd of goats (200 transgenic females) that produced recombinant human lactoferrin (rhLF) in their milk at levels up to 16 g/L was obtained. The rhLF from milk of transgenic goats, natural human lactoferrin (hLF) from woman milk and natural goat lactoferrin (gLF) from milk of non-transgenic goats were purified using cation-exchange chromatography. It has been shown that rhLF is a glycoprotein and its physicochemical characteristics of rhLF are similar to hLf as revealed by different analytical methods including electron paramagnetic resonance, spectrophotometry, differential scanning calorimetry, mass spectrometry and peptide mapping. The high expression level of rhLF achieved in milk of transgenic goats provides a solid basis for developing an efficient and cost-effective downstream processing. The rhLF exhibited a prominent biological activity suggesting it as a promising biopharmaceutical and food supplements.

2-(1H-tetrazol-1-yl)benzoic acid.

In the title compound, C8H6N4O2, the tetrazole and benzene rings are planar to within 0.001 (1) and 0.007 (1) A, respectively. These rings are not coplanar in the molecule, the dihedral angle between them being 52.90 (4) degrees. Molecules are connected together by O-H...N and C-H...O hydrogen bonds, forming two-dimensional networks parallel to the xz plane with van der Waals interactions between them.

[Periodontal diseases in iron-deficiency states]. / Zabolevaniia parodonta pri zhelezodefitsitnykh sostoianiiakh.

Dentists should be on the alert when treating periodontal diseases in risk-group subjects. A manifest gingival inflammation along with other signs of iron-deficiency state, coupled with no effect of stomatological treatment, give grounds to recommend these patients to consult a hematologist.

[Clinico-experimental research on the use of collagen in periodontal disease]. / Kliniko-éksperimental'nye issledovaniia primeneniia kollagena pri zabolevanii parodonta.

Clinico-experimental studies of a new collagen derivative are presented. Experimental investigation in 30 rats showed the substance to promote wound healing. It also proved efficient in the five-years clinical follow-up of 254 patients after 441 surgical interventions (generalized periodontitis).