RESUMEN
Cuscuta and Cassytha are two distinct stem parasitic plant genera developing haustoria at their stem. The initial step to parasitization is twining onto the host plant. Although twining is the critical first step, less attention has been paid to this aspect in stem haustoria parasitic plant studies. As tendril coiling is also controlled by light and plant hormones, we investigated the role of light (blue, red and far-red) and hormones (auxin, brassinolide, cytokinin) in twining of stem parasitic plants (Cuscuta japonica and Cassytha filiformis). In general, both Cuscuta and Cassytha showed similar behavior to light cues. The data show that blue light is essential for twining, and a lower far-red/red light (FR/R) ratio is important for subsequent haustoria induction. Regarding plant hormones, seedlings with solely auxin or cytokinin (iP) under blue light showed not only twining but also haustoria induction, demonstrating that auxin and iP appear to be especially important for induction. Seedlings with solely brassinolide showed no positive influence, but brassinolide together with iP caused twining even under dark conditions. This points to the presence of cross-talk between brassinolide and cytokinin for twining.
Asunto(s)
Cuscuta , Regulación de la Expresión Génica de las Plantas , Reguladores del Crecimiento de las Plantas , Plantas , PlantonesRESUMEN
Background: Achieving clear visibility through a windshield is one of the crucial factors in manufacturing a safe and comfortable vehicle. The optic flow (OF) through the windshield has been reported to divert attention and could impair visibility. Although a growing number of behavioral and neuroimaging studies have assessed drivers' attention in various driving scenarios, there is still little evidence of a relationship between OF, windshield shape, and driver's attentional efficacy. The purpose of this research was to examine this relationship. Methods: First, we quantified the OF across the windshield in a simulated driving scenario with either of two types of the windshield (a tilted or vertical pillar) at different speeds (60 km/h or 160 km/h) and found more upward OF along the tilted pillar than along the vertical pillar. Therefore, we hypothesized that the predominance of upward OF around the windshield along a tilted pillar could distract a driver and that we could observe the corresponding neural activity. Magnetic resonance scans were then obtained while the subjects performed a visual detection task while watching the driving scene used in the OF analysis. The subjects were required to press a button as rapidly as possible when a target appeared at one of five positions (leftmost, left, center, right, and rightmost). Results: We found that the reaction time (RT) on exposure to a tilted pillar was longer than that on exposure to a vertical pillar in the leftmost and rightmost conditions. Furthermore, there was more brain activity in the precuneus when the pillar was tilted than when it was vertical in the rightmost condition near the pillar. In a separate analysis, activation in the precuneus was found to reflect relative changes in the amount of upward OF when the target was at the rightmost position. Conclusions: Overall, these observations suggest that activation in the precuneus may reflect extraneous cognitive load driven by upward OF along the pillar and could distract visual attention. The findings of this study highlight the value of a cognitive neuroscientific approach to research and development in the motor vehicle manufacturing industry.
RESUMEN
Cuscuta and Cassytha are two well-known stem parasitic plant genera with reduced leaves and roots, inducing haustoria in their stems. Their similar appearance in the field has been recognized, but few comparative studies on their respective plant interactions are available. To compare their interactions, we conducted a metabolite analysis of both the Cassytha-Ipomoea and the Cuscuta-Momordica interaction. We investigated the energy charge of the metabolites by UFLC (ultra-high performance liquid chromatography), and conducted GC-MS (gas chromatography-mass spectrometry) analysis for polar metabolites (e.g., saccharides, polyols) and steroids. The energy charge after parasitization changed considerably in Cassytha but not in Cusucta. Cuscuta changed its steroid pattern during the plant interaction, whereas Cassytha did not. In the polar metabolite analysis, the laminaribiose increase after parasitization was conspicuous in Cuscuta, but not in Cassytha. This metabolite profile difference points to different lifestyles and parasitic strategies.
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PREMISE OF THE STUDY: Few previous studies have examined how mycobionts change during the evolution from autotrophy to mycoheterotrophy based on phylogenetic hypotheses. Neottia (Orchidaceae) comprises leafy species that are autotrophic and related leafless mycoheterotrophic species, and the phylogenetic relationships among them have been clarified. Accordingly, Neottia is a suitable taxon for investigating the question above. Here we clarified the diversity of mycobionts in Neottia plants and elucidated changes in the character of symbiotic associations during the evolution of mycoheterotrophy. METHODS: We sequenced the internal transcribed spacer (ITS) regions of nuclear ribosomal (nr) DNA for mycobionts of Neottia plants. Furthermore, we selected one representative DNA sample from each fungal operational taxonomic unit (OTU) and used it to amplify the large subunit (LSU) nrDNA sequences. Phylogenetic analyses of Sebacinales (basidiomycetes), the dominant mycobiont of Neottia, were conducted and sample-based rarefaction curves generated for the observed mycobiont richness on each OTU. KEY RESULTS: Leafy and leafless species in Neottia were associated with Sebacinales Group B and Sebacinales Group A, respectively. The composition and specificity level of fungal partners varied among Neottia species. CONCLUSIONS: Fungal partner composition and specificity level changed with speciation in both leafy and leafless Neottia species. In particular, mycorrhizal associations likely shifted from Sebacinales Group B to Group A during the evolution from autotrophy to mycoheterotrophy. Partner shifts to Sebacinales Group A have also been reported in the evolution of mycoheterotrophy of other plant groups, suggesting that convergence to this fungal group occurs in association with the evolution of mycoheterotrophy.
Asunto(s)
Basidiomycota/fisiología , Evolución Biológica , Orchidaceae/microbiología , Simbiosis , Basidiomycota/genética , ADN de Hongos/genética , ADN Ribosómico/genética , ADN Espaciador Ribosómico/genética , Micorrizas/fisiología , FilogeniaRESUMEN
PREMISE OF THE STUDY: Photosynthetic orchids found in highly shaded forests are often mixotrophic, receiving part of their carbon energy via ectomycorrhizal fungi that had originally received carbohydrate from trees. A photosynthetic orchid, Cremastra appendiculata, is also found under highly shaded forest, but our preliminary data suggested that its associated fungi were not ectomycorrhizal. We tested whether their relation is an unusual example of a mixotrophic orchid associating with saprotrophic fungi by direct detection of fungal DNAs in conjunction with isolation of the fungus in pure culture and experimental inoculation of orchid seeds with the fungus. ⢠METHODS: For isolated mycobionts of C. appendiculata plants, two regions of nuclear ribosomal DNA, the internal transcribed spacer (ITS) and the large subunit (LSU), were sequenced, and fruiting bodies of the one isolate, SI1-1 were induced. In addition, two fungal isolates, SI1-1 and KI1-1, were grown in symbiotic cultures with C. appendiculata to verify their status as mycobionts. ⢠KEY RESULTS: In phylogenetic analyses, all isolates clustered with fungi belonging to Coprinellus in Psathyrellaceae of Agaricales. Phylogenetic analyses of these DNA sequences showed that five fungal isolates from C. appendiculata, including SI1-1 and two mycobionts isolated from the mycoheterotrophic orchid Epipogium roseum, have very similar ITS sequences. Isolate SI1-1 was identified as Coprinellus domesticus based on the morphological characteristics of the fruiting body. Isolates SI1-1 and KI1-1 induced seed germination of C. appendiculata as mycobionts. ⢠CONCLUSIONS: This report is the first of a mycorrhizal symbiosis between a fungus in Psathyrellaceae and a photosynthetic orchid, revealing a new pathway to full mycoheterotrophy and contributing to our understanding of the evolution of mycoheterotrophy.
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Agaricales/clasificación , Micorrizas/clasificación , Orchidaceae/microbiología , Simbiosis , Agaricales/citología , Agaricales/genética , Agaricales/fisiología , Evolución Biológica , ADN de Hongos/química , ADN de Hongos/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Cuerpos Fructíferos de los Hongos , Hifa , Micorrizas/citología , Micorrizas/genética , Micorrizas/fisiología , Orchidaceae/anatomía & histología , Orchidaceae/fisiología , Fotosíntesis , Filogenia , Raíces de Plantas/anatomía & histología , Raíces de Plantas/microbiología , Raíces de Plantas/fisiología , Brotes de la Planta/anatomía & histología , Brotes de la Planta/microbiología , Brotes de la Planta/fisiología , Rizoma/anatomía & histología , Rizoma/microbiología , Rizoma/fisiología , Plantones/anatomía & histología , Plantones/microbiología , Plantones/fisiología , Análisis de Secuencia de ADN , ÁrbolesRESUMEN
We examined the colonization rate and communities of arbuscular mycorrhizal fungi (AMF) in the roots of Pyrus pyrifolia var. culta (Japanese pear) in orchards to investigate the effect of phosphorus (P) fertilization on AMF. Soil cores containing the roots of Japanese pear were collected from 13 orchards in Tottori Prefecture, Japan. Soil-available P in the examined orchards was 75.7 to 1,200 mg kg(-1), showing the extreme accumulation of soil P in many orchards. The AMF colonization rate was negatively correlated with soil-available P (P <0.01). AMF communities were examined on the basis of the partial fungal DNA sequences of the nuclear small-subunit ribosomal RNA gene (SSU rDNA) amplified by AMF-specific primers AML1 and AML2. The obtained AMF sequences were divided into 14 phylotypes, and the number of phylotypes (species richness) was also negatively correlated with soil-available P (P <0.05). It was also suggested that some AM fungi may be adapted to high soil-available P conditions. Redundancy analysis showed the significant effects of soil pH, available P in soil, and P content in leaves of P. pyrifolia var. culta trees on AMF distribution. These results suggested that the accumulation of soil-available P affected AMF communities in the roots of Japanese pear in the orchard environment.
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Hongos/crecimiento & desarrollo , Hongos/genética , Micorrizas/crecimiento & desarrollo , Fósforo/farmacología , Raíces de Plantas/microbiología , Pyrus/microbiología , Microbiología del Suelo , ADN de Hongos/análisis , ADN de Hongos/genética , Ecosistema , Hongos/clasificación , Datos de Secuencia Molecular , Micorrizas/clasificación , Micorrizas/efectos de los fármacos , Micorrizas/genética , Fósforo/análisis , Raíces de Plantas/efectos de los fármacos , Pyrus/crecimiento & desarrollo , Análisis de Secuencia de ADN , Suelo/químicaRESUMEN
The community structure of arbuscular mycorrhizal (AM) fungi associated with Ixeris repens was studied in coastal vegetation near the Tottori sand dunes in Japan. I. repens produces roots from a subterranean stem growing near the soil surface which provides an opportunity to examine the effects of an environmental gradient related to distance from the sea on AM fungal communities at a regular soil depth. Based on partial sequences of the nuclear large subunit ribosomal RNA gene, AM fungi in root samples were divided into 17 phylotypes. Among these, five AM fungal phylotypes in Glomus and Diversispora were dominant near the seaward forefront of the vegetation. Redundancy analysis of the AM fungal community showed significant relationships between the distribution of phylotypes and environmental variables such as distance from the sea, water-soluble sodium in soil, and some coexisting plant species. These results suggest that environmental gradients in the coastal vegetation can be determinants of the AM fungal community.
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Asteraceae/microbiología , Glomeromycota/aislamiento & purificación , Micorrizas/aislamiento & purificación , Secuencia de Bases , Convolvulaceae/microbiología , Cyperaceae/microbiología , ADN de Hongos/química , ADN de Hongos/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Ambiente , Glomeromycota/clasificación , Glomeromycota/genética , Glomeromycota/crecimiento & desarrollo , Japón , Datos de Secuencia Molecular , Micorrizas/clasificación , Micorrizas/genética , Micorrizas/crecimiento & desarrollo , Océanos y Mares , Fosfatos/análisis , Filogenia , Raíces de Plantas/microbiología , Tallos de la Planta/microbiología , Poaceae/microbiología , Salinidad , Análisis de Secuencia de ADN , Sodio/análisis , Suelo/química , SimbiosisRESUMEN
Lecanorchis is a nonphotosynthetic plant genus in Vanilloideae, Orchidaceae. Because of the distribution of many Lecanorchis taxa in various climate conditions, we hypothesized that mycorrhizal diversity and specificity are different among the different taxa of Lecanorchis. In the present study, identities of mycorrhizal fungi were examined for 90 individuals of 10 Lecanorchis taxa at 26 sites from Niigata to Okinawa Prefectures in Japan. Phylogenetic analyses of Lecanorchis taxa based on the internal transcribed spacer (ITS) region of the nuclear ribosomal RNA gene (rDNA) divided the examined Lecanorchis taxa into three groups, groups A, B, and C. ITS rDNA sequences suggested that fungi associating with Lecanorchis were ectomycorrhiza-forming fungi in Lactarius, Russula, Atheliaceae, and Sebacina, with Lactarius and Russula dominant. Our results suggested some degree of mycorrhizal specialization among Lecanorchis taxa. Interestingly, the Lecanorchis group C had some specific relationships with Lactarius, whereas less specificity was found in the relationships with Russula. However, observed specificity results may be biased by geographic opportunity, and we suggest further research to assess whether Lecanorchis species are limited to the associations we observed.
Asunto(s)
Micorrizas/aislamiento & purificación , Orchidaceae/microbiología , Secuencia de Bases , Teorema de Bayes , ADN de Hongos/genética , ADN de Plantas/genética , ADN Espaciador Ribosómico/genética , ADN Espaciador Ribosómico/metabolismo , Bases de Datos Genéticas , Genes de ARNr , Variación Genética , Japón , Micorrizas/clasificación , Micorrizas/genética , Orchidaceae/clasificación , Orchidaceae/genética , Filogenia , Raíces de Plantas/genética , Raíces de Plantas/metabolismo , Raíces de Plantas/microbiología , Tallos de la Planta/genética , Alineación de Secuencia , Análisis de Secuencia de ADN , Especificidad de la Especie , SimbiosisRESUMEN
⢠We investigated the fungal symbionts and carbon nutrition of a Japanese forest photosynthetic orchid, Platanthera minor, whose ecology suggests a mixotrophic syndrome, that is, a mycorrhizal association with ectomycorrhiza (ECM)-forming fungi and partial exploitation of fungal carbon. ⢠We performed molecular identification of symbionts by PCR amplifications of the fungal ribosomal DNA on hyphal coils extracted from P. minor roots. We tested for a (13)C and (15)N enrichment characteristic of mixotrophic plants. We also tested the ectomycorrhizal abilities of orchid symbionts using a new protocol of direct inoculation of hyphal coils onto roots of Pinus densiflora seedlings. ⢠In phylogenetic analyses, most isolated fungi were close to ECM-forming Ceratobasidiaceae clades previously detected from a few fully heterotrophic orchids or environmental ectomycorrhiza surveys. The direct inoculation of fungal coils of these fungi resulted in ectomycorrhiza formation on P. densiflora seedlings. Stable isotope analyses indicated mixotrophic nutrition of P. minor, with fungal carbon contributing from 50% to 65%. ⢠This is the first evidence of photosynthetic orchids associated with ectomycorrhizal Ceratobasidiaceae taxa, confirming the evolution of mixotrophy in the Orchideae orchid tribe, and of ectomycorrhizal abilities in the Ceratobasidiaceae. Our new ectomycorrhiza formation technique may enhance the study of unculturable orchid mycorrhizal fungi.
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Basidiomycota/fisiología , Micorrizas/fisiología , Orchidaceae/microbiología , Orchidaceae/fisiología , Procesos Autotróficos/fisiología , Secuencia de Bases , Basidiomycota/citología , Basidiomycota/genética , Teorema de Bayes , Isótopos de Carbono , ADN Intergénico/genética , Ecosistema , Cuerpos Fructíferos de los Hongos/citología , Cuerpos Fructíferos de los Hongos/fisiología , Japón , Datos de Secuencia Molecular , Micorrizas/citología , Micorrizas/genética , Isótopos de Nitrógeno , Orchidaceae/citología , FilogeniaRESUMEN
Mycorrhizal fungi in roots of the achlorophyllous Petrosavia sakuraii (Petrosaviaceae) were identified by molecular methods. Habitats examined were plantations of the Japanese cypress Chamaecyparis obtusa in Honshu, an evergreen broad-leaved forest in Amami Island in Japan and a mixed deciduous and evergreen forest in China. Aseptate hyphal coils were observed in root cortical cells of P. sakuraii, suggesting Paris-type arbuscular mycorrhiza (AM). Furthermore, hyphal coils that had degenerated to amorphous clumps were found in various layers of the root cortex. Despite extensive sampling of P. sakuraii from various sites in Japan and China, most of the obtained AM fungal sequences of the nuclear small subunit ribosomal RNA gene were nearly identical and phylogenetic analysis revealed that they formed a single clade in the Glomus group A lineage. This suggests that the symbiotic relationship is highly specific. AM fungi of P. sakuraii were phylogenetically different from those previously detected in the roots of some mycoheterotrophic plants. In a habitat in C. obtusa plantation, approximately half of the AM fungi detected in roots of C. obtusa surrounding P. sakuraii belonged to the same clade as that of P. sakuraii. This indicates that particular AM fungi are selected by P. sakuraii from diverse indigenous AM fungi. The same AM fungi can colonize both plant species, and photosynthates of C. obtusa may be supplied to P. sakuraii through a shared AM fungal mycelial network. Although C. obtusa plantations are widely distributed throughout Japan, P. petrosavia is a rare plant species, probably because of its high specificity towards particular AM fungi.
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Glomeromycota/clasificación , Glomeromycota/aislamiento & purificación , Magnoliopsida/microbiología , Micorrizas/clasificación , Micorrizas/aislamiento & purificación , China , Análisis por Conglomerados , ADN de Hongos/química , ADN de Hongos/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Genes de ARNr , Glomeromycota/genética , Glomeromycota/fisiología , Japón , Magnoliopsida/fisiología , Datos de Secuencia Molecular , Micorrizas/genética , Micorrizas/fisiología , Filogenia , Raíces de Plantas/microbiología , ARN de Hongos/genética , ARN Ribosómico 18S/genética , Análisis de Secuencia de ADN , SimbiosisRESUMEN
During taxonomic revision of genus Octaviania in Japan we examined herbarium and fresh specimens of O. columellifera and O. asterosperma sensu S. Yoshimi & Y. Doi with morphological and molecular techniques. These two species were identical in both macro- and micromorphological characters and were clearly different from the generally known O. asterosperma. The identity of the two species and their distinctness from O. asterosperma was further supported by both nuclear large subunit and ITS rDNA phylogeny. The molecular analyses also revealed that O. columellifera shares its lineage with the boletoid mushroom-forming Xerocomus chrysenteron complex and that it does not form a monophyletic clade with other Octaviania species. Our morphological reevaluation, including transmission electron microscopic observation of basidiospores, clarified the taxonomic boundary between O. columellifera and other Japanese Octaviania species. Accordingly we propose a new genus, Heliogaster, for O. columellifera with designation of the lectotype. We discuss phylogenetic relationships with Octaviania sensu stricto species and the closely related boletoid (pileate-stipitate) fungi, generic characters of Heliogaster and intraspecific phylogeny.
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Basidiomycota/clasificación , Filogenia , Basidiomycota/genética , Basidiomycota/ultraestructura , ADN de Hongos/análisis , ADN Espaciador Ribosómico/análisis , Japón , Datos de Secuencia Molecular , Técnicas de Tipificación Micológica , Análisis de Secuencia de ADN , Especificidad de la Especie , Esporas Fúngicas/ultraestructuraRESUMEN
BACKGROUND AND AIMS: Epipogium aphyllum is a Eurasian achlorophyllous, mycoheterotrophic forest orchid. Due to its rarity, it is often protected, and its biology is poorly known. The identity and pattern of colonization of fungal associates providing carbon to this orchid have not been studied previously. METHODS: Using samples from 34 individuals from 18 populations in Japan, Russia and France, the following were investigated: (a) colonization patterns of fungal associates of E. aphyllum by microscopy; (b) their identity by PCR amplification of nuclear ribosomal ITS carried out on rhizome fragments and hyphal pelotons. RESULTS AND CONCLUSIONS: Microscopic investigations revealed that thick rhizomes were densely colonized by fungi bearing clamp-connections and dolipores, i.e. basidiomycetes. Molecular analysis identified Inocybe species as exclusive symbionts of 75 % of the plants investigated and, more rarely, other basidiomycetes (Hebeloma, Xerocomus, Lactarius, Thelephora species). Additionally, ascomycetes, probably endophytes or parasites, were sometimes present. Although E. aphyllum associates with diverse species from Inocybe subgenera Mallocybe and Inocybe sensu stricto, no evidence for cryptic speciation in E. aphyllum was found. Since basidiomycetes colonizing the orchid are ectomycorrhizal, surrounding trees are probably the ultimate carbon source. Accordingly, in one population, ectomycorrhizae sampled around an individual orchid revealed the same fungus on 11.2 % of tree roots investigated. Conversely, long, thin stolons bearing bulbils indicated active asexual multiplication, but these propagules were not colonized by fungi. These findings are discussed in the framework of ecology and evolution of mycoheterotrophy.
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Procesos Heterotróficos , Micorrizas/fisiología , Orchidaceae/microbiología , Reproducción Asexuada , Basidiomycota/crecimiento & desarrollo , Recuento de Colonia Microbiana , Orchidaceae/anatomía & histología , Filogenia , Rizoma/anatomía & histología , Rizoma/microbiologíaRESUMEN
Community of arbuscular mycorrhizal (AM) fungi in a coastal vegetation on Okinawa island in Japan was examined. A sampling plot was established in a colony of Ipomoea pes-caprae (Convolvulaceae) on the beach in Tamagusuku, Okinawa Pref, in which eight root samples of I. pes-caprae and three root samples each of Vigna marina (Leguminosae) and Paspalum distichum (Poaceae) were collected. Partial 18S rDNA of AM fungi was amplified from the root samples by polymerase chain reaction (PCR) with primers NS31 and AM1. Restriction fragment length polymorphism analysis with HinfI and RsaI for cloned PCR products revealed that two types of Glomus sp., type A and type B, were dominant in the colony. Among them, the fungi of type A were especially dominant near the edge of the colony facing the sea. A phylogenetic analysis showed that the AM fungi of type B are closely related to Glomus intraradices and those of type A are nearly related to type B. From the sequence data, it was also found that type A was further divided into two types, type A1 and A2. One representative strain each of the three types, type A1, A2, and B, propagated from single spore each, was examined for the growth of sorghum (Sorghum bicolor) at three different salinity levels, 0, 100, and 200 mM NaCl. At the non-salt-treated condition, the type B fungus was the most effective on shoot growth enhancement of the host plant, whereas at the salt-treated conditions, the type A2 fungus was the most effective. An efficient suppression of Na + translocation into the shoot by the examined AM fungi was found. These results suggested that the AM fungi dominant near the sea are adapted to salt-stressed environment to alleviate the salt stress of host plants.
Asunto(s)
Hongos/aislamiento & purificación , Ipomoea/microbiología , Micorrizas/aislamiento & purificación , Cloruro de Sodio/farmacología , Microbiología del Suelo , Sorghum/crecimiento & desarrollo , Tamaño de la Célula , ADN de Hongos/genética , ADN Ribosómico/genética , Hongos/clasificación , Hongos/genética , Hongos/metabolismo , Japón , Datos de Secuencia Molecular , Micorrizas/clasificación , Micorrizas/genética , Micorrizas/metabolismo , Filogenia , Polimorfismo de Longitud del Fragmento de Restricción , ARN Ribosómico 18S/genética , Cloruro de Sodio/metabolismo , Suelo/análisis , Sorghum/efectos de los fármacos , Sorghum/microbiología , Esporas Fúngicas/química , Esporas Fúngicas/citologíaRESUMEN
Mycorrhizal fungi were isolated from the nonphotosynthetic orchid Chamaegastrodia sikokiana and identified as members of Ceratobasidiaceae by phylogenetic analysis of the internal transcribed spacer (ITS) region of ribosomal deoxyribonucleic acid. The ITS sequences were similar among geographically separated samples obtained from Mt. Kiyosumi in Chiba Prefecture and Mt. Yokokura in Kochi Prefecture. One of the isolated fungi, KI1-2, formed ectomycorrhiza on seedlings of Abies firma in pot culture, suggesting that tripartite symbiosis exists among C. sikokiana, mycorrhizal fungi, and A. firma in nature, and carbon compounds are supplied from A. firma to C. sikokiana through the hyphae of the mycorrhizal fungi. To our knowledge, this is the second study to suggest the involvement of Ceratobasidiaceae fungi in tripartite symbiosis with achlorophyllous orchids and photosynthetic host plants.
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Basidiomycota/aislamiento & purificación , Micorrizas/crecimiento & desarrollo , Orchidaceae/microbiología , Abies/microbiología , Basidiomycota/clasificación , Basidiomycota/genética , ADN de Hongos/química , ADN de Hongos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Japón , Datos de Secuencia Molecular , Filogenia , Raíces de Plantas/microbiología , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido Nucleico , SimbiosisRESUMEN
We have achieved the symbiotic cultivation of an apparently achlorophyllous orchid, Epipogium roseum Lindl., with a mycorrhizal fungus isolated from an underground organ of this orchid. Although the seed germination rate was extremely low, subsequent growth from protocorm to flowering was induced in a medium containing volcanic soils and sawdust. Stolons elongated from each protocorm, and rhizomes were formed at certain intervals on the stolons. Some of the rhizomes developed into a coralloid form, and tubers were formed from the coralloid rhizomes. The coralloid rhizomes degenerated concurrently with maturation of the tubers. Six months after seed sowing, around 80 tubers were produced from a single protocorm. An inflorescence appeared from each of the large tubers, and the process to flowering was observed in one of these. Consequently, the developmental processes from seed to flowering in E. roseum was clearly revealed in this study.