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Cover cropping is a promising strategy to improve soil health, but it may also trigger greenhouse gas emissions, especially nitrous oxide (N2O). Beyond nitrogen (N) availability, cover crop residue decomposition may accelerate heterotrophic respiration to limit soil O2 availability, hence promote N2O emissions from denitrification under sub-optimal water-filled pore space (WFPS) conditions that are typically not conducive to large N2O production. We conducted a 21-day incubation experiment to examine the effects of contrasting cover crop residue (grass vs legume) decomposition on soil O2 and biogeochemical changes to influence N2O and CO2 emissions from 15N labeled fertilized soils under 50% and 80% WFPS levels. Irrespective of cover crop type, mixing cover crop residue with N fertilizer resulted in high cumulative N2O emissions under both WFPS conditions. In the absence of cover crop residues, the N fertilizer effect of N2O was only realized under 80% WFPS, whereas it was comparable to the control under 50% WFPS. The N2O peaks under 50% WFPS coincided with soil O2 depletion and concomitant high CO2 emissions when cover crop residues were mixed with N fertilizer. While N fertilizer largely contributed to the total N2O emissions from the cover crop treatments, soil organic matter and/or cover crop residue derived N2O had a greater contribution under 50% than 80% WFPS. Our results underscore the importance of N2O emissions from cover crop-based fertilized systems under relatively lower WFPS via a mechanism of respiration-induced anoxia and highlight potential risks of underestimating N2O emissions under sole reliance on WFPS.
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Microscale alterations in soil physical characteristics resulting from long-term soil health practices can contribute to changes in soil nitrous oxide (N2O) emissions. In this study, we investigated soil N2O emissions in relation to pore characteristics influencing soil gas diffusivity under long-term tillage and cover cropping practices. Intact soil cores from tillage (conventional tillage, Conv. T versus no tillage, NT) and cover crop (hairy vetch, HV versus no cover crop, NC) treatments were used for N2O measurements and computed tomography (CT) scanning. Using X-ray CT technique with a resolution of 59 µm, pore structure parameters including macroporosity, number of macropores, anisotropy, fractal dimension, tortuosity, and connectivity were determined. The results showed that Conv. T and HV emitted significantly higher N2O than NT and NC, respectively. A similar trend was observed for macroporosity, Conv. T soils had 27.4 % higher CT-derived macroporosity than the NT soils and HV increased macroporosity by 31.1 % over the NC treatment. The number of macropores and fractal dimension were significantly higher whereas degree of anisotropy was significantly lower under HV compared to NC. In the upper 3 cm of soil, HV had a connected porosity, whereas the pores were disconnected and isolated in NC. These CT-derived properties; however, were not impacted by tillage treatments. N2O emissions were positively and significantly correlated to relative soil gas diffusivity, CT-derived macroporosity, number of macropores, and fractal dimension. Our results demonstrated that soil macroporosity and relative gas diffusivity could lead to improved understanding and predictability of N2O emissions under high soil moisture conditions.
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Carbon amendments designed to remediate environmental contamination lead to substantial perturbations when injected into the subsurface. For the remediation of uranium contamination, carbon amendments promote reducing conditions to allow microorganisms to reduce uranium to an insoluble, less mobile state. However, the reproducibility of these amendments and underlying microbial community assembly mechanisms have rarely been investigated in the field. In this study, two injections of emulsified vegetable oil were performed in 2009 and 2017 to immobilize uranium in the groundwater at Oak Ridge, TN, USA. Our objectives were to determine whether and how the injections resulted in similar abiotic and biotic responses and their underlying community assembly mechanisms. Both injections caused similar geochemical and microbial succession. Uranium, nitrate, and sulfate concentrations in the groundwater dropped following the injection, and specific microbial taxa responded at roughly the same time points in both injections, including Geobacter, Desulfovibrio, and members of the phylum Comamonadaceae, all of which are well established in uranium, nitrate, and sulfate reduction. Both injections induced a transition from relatively stochastic to more deterministic assembly of microbial taxonomic and phylogenetic community structures based on 16S rRNA gene analysis. We conclude that geochemical and microbial successions after biostimulation are reproducible, likely owing to the selection of similar phylogenetic groups in response to EVO injection.
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Woodchip bioreactor (WBR) is commonly used to remove nitrate from drainage and runoff. However, the efficiency of nitrate removal in WBR is highly variable due to the properties of filling materials. In this study, we investigated the potential of recycling two waste materials, biochar (B) and silage leachate (SL), to enhance nitrate removal by providing a better living habitat and extra available carbon for denitrification. We constructed twelve lab-scale bioreactors with different filling materials (WBR, WBR + B, WBR + SL, WBR + B + SL), hydraulic retention times (HRT: 0.5-24 h), and nitrate concentrations (5.4-33 mg L-1) to test nitrate removal efficiency (NRE) and nitrate removal rate (NRR). Our results showed that the combination of biochar and silage leachate led to the highest NRE and NRR, with improvements of 23% and 48%, respectively, compared to WBR alone. However, the benefits of adding biochar and silage leachate were less apparent at longer HRTs. According to the results of our structural equation modeling (SEM), we have attributed the improved denitrification to several factors. These factors include the decrease in dissolved oxygen, saturated hydraulic conductivity, and pH value, as well as an increase in dissolved organic carbon after the addition of silage leachate. Therefore, our study provides evidence that recycling biochar and silage leachate as an additive to WBR could be a beneficial strategy for enhancing nitrate removal. Overall, this study highlights the potential of a win-win solution to improve the efficiency of nitrate removal in water treatment processes.
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Desnitrificación , Nitratos , Ensilaje , Reactores Biológicos , NitrógenoRESUMEN
Microbial assembly and metabolic potential in the subsurface critical zone (SCZ) are substantially impacted by subsurface geochemistry and hydrogeology, selecting for microbes distinct from those in surficial soils. In this study, we integrated metagenomics and geochemistry to elucidate how microbial composition and metabolic potential are shaped and impacted by vertical variations in geochemistry and hydrogeology in terrestrial subsurface sediment. A sediment core from an uncontaminated, pristine well at Oak Ridge Field Research Center in Oak Ridge, Tennessee, including the shallow subsurface, vadose zone, capillary fringe, and saturated zone, was used in this study. Our results showed that subsurface microbes were highly localized and that communities were rarely interconnected. Microbial community composition as well as metabolic potential in carbon and nitrogen cycling varied even over short vertical distances. Further analyses indicated a strong depth-related covariation of community composition with a subset of 12 environmental variables. An analysis of dissolved organic carbon (DOC) quality via ultrahigh resolution mass spectrometry suggested that the SCZ was generally a low-carbon environment, with the relative portion of labile DOC decreasing and that of recalcitrant DOC increasing along the depth, selecting microbes from copiotrophs to oligotrophs and also impacting the microbial metabolic potential in the carbon cycle. Our study demonstrates that sediment geochemistry and hydrogeology are vital in the selection of distinct microbial populations and metabolism in the SCZ. IMPORTANCE In this study, we explored the links between geochemical parameters, microbial community structure and metabolic potential across the depth of sediment, including the shallow subsurface, vadose zone, capillary fringe, and saturated zone. Our results revealed that microbes in the terrestrial subsurface can be highly localized, with communities rarely being interconnected along the depth. Overall, our research demonstrates that sediment geochemistry and hydrogeology are vital in the selection of distinct microbial populations and metabolic potential in different depths of subsurface terrestrial sediment. Such studies correlating microbial community analyses and geochemistry analyses, including high resolution mass spectrometry analyses of natural organic carbon, will further the fundamental understanding of microbial ecology and biogeochemistry in subsurface terrestrial ecosystems and will benefit the future development of predictive models on nutrient turnover in these environments.
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Bacterias , Microbiota , Bacterias/metabolismo , Carbono/metabolismo , TennesseeRESUMEN
Soil microbial transformation of nitrogen (N) in nutrient-limited native C4 grasslands can be affected by N fertilization rate and C4 grass species. Here, we report in situ dynamics of the population size (gene copy abundances) and activity (transcript copy abundances) of five functional genes involved in soil N cycling (nifH, bacterial amoA, nirK, nirS, and nosZ) in a field experiment with two C4 grass species (switchgrass (Panicum virgatum) and big bluestem (Andropogon gerardii)) under three N fertilization rates (0, 67, and 202 kg N ha-1). Diazotroph (nifH) abundance and activity were not affected by N fertilization rate nor grass species. However, moderate and high N fertilization promoted population size and activity of ammonia oxidizing bacteria (AOB, quantified via amoA genes and transcripts) and nitrification potential. Moderate N fertilization increased abundances of nitrite-reducing bacterial genes (nirK and nirS) under switchgrass but decreased these genes under big bluestem. The activity of nitrous oxide reducing bacteria (nosZ transcripts) was also promoted by moderate N fertilization. In general, high N fertilization had a negative effect on N-cycling populations compared to moderate N addition. Compared to big bluestem, the soils planted with switchgrass had a greater population size of AOB and nitrite reducers. The significant interaction effects of sampling season, grass species, and N fertilization rate on N-cycling microbial community at genetic-level rather than transcriptional-level suggested the activity of N-cycling microbial communities may be driven by more complex environmental factors in native C4 grass systems, such as climatic and edaphic factors.
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Pradera , Urea , Poaceae , Nitritos , Bacterias/genética , Suelo , Nitrógeno/farmacología , FertilizaciónRESUMEN
Woodchip bioreactor (WBR) is one of the green infrastructures in the agriculture system to reduce nitrate from agricultural drainage and stormwater. A lot of abiotic factors have been reported that affect nitrate removal lacking a comprehensive understanding. In this study, we studied eight important abiotic factors, including media age, hydraulic retention time (HRT), influent nitrate concentration (Cin), temperature, dissolved organic carbon (DOC), dissolved oxygen (DO), pH, and effective porosity (ρe) of WBR-filling materials. Based on a database that included 10,179 sets of data from 63 peer-reviewed articles, the nitrate removal rate (NRR) and nitrate removal efficiency (NRE) corresponding to the eight abiotic factors by different categories were comprehensively reported. According to this database, this study found the optimal range of abiotic factors for NRR and NRE in WBR were different. Regarding NRR, the optimal range of media age, HRT, Cin, temperature, effluent DOC, DO, pH, and ρe were in the first year, 0-5 h, 10-20 mg L-1, 20-25 °C, 0-5 mg L-1, 0-0.5 mg L-1, 7-8, and 0.6-0.7, respectively. For NRE, the optimal range of media age, HRT, Cin, temperature, effluent DOC, DO, pH, and ρe were in the first year, 500-3000 h, 0-10 mg L-1, 10-15 °C, >50 mg L-1, 0-0.5 mg L-1, 4-5, and 0.4-0.5, respectively. Moreover, the principal component analysis (PCA) indicated the field studies' principal components were different from laboratory studies. Furthermore, the structural equation modeling (SEM) also revealed the causal relationship of the eight abiotic factors on NRR and NRE is totally different. Lessons from this study can be incorporated into DNBR designs, especially improving nitrate removal rates by optimizing different abiotic factors. It also provides insights regarding the contributions of different abiotic factors for NRR and NRE independently and comprehensively.
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Desnitrificación , Nitratos , Reactores Biológicos , Nitratos/análisis , Óxidos de Nitrógeno , OxígenoRESUMEN
Interactions between species above- and belowground are among the top factors that govern ecosystem functioning including soil organic carbon (SOC) storage. In agroecosystems, understanding how crop diversification affects soil biodiversity and SOC storage at the local scale remains a key challenge for addressing soil degradation and biodiversity loss that plague these systems. Yet, outcomes of crop diversification for soil microbial diversity and SOC storage, which are key indicators of soil health, are not always positive but rather they are highly idiosyncratic to agroecosystems. Using five case studies, we highlight the importance of selecting ideal crop functional types (as opposed to focusing on plant diversity) when considering diversification options for maximizing SOC accumulation. Some crop functional types and crop diversification approaches are better suited for enhancing SOC at particular sites, though SOC responses to crop diversification can vary annually and with duration of crop cover. We also highlight how SOC responses to crop diversification are more easily interpretable through changes in microbial community composition (as opposed to microbial diversity). We then develop suggestions for future crop diversification experiment standardization including (1) optimizing sampling effort and sequencing depth for soil microbial communities and (2) understanding the mechanisms guiding responses of SOC functional pools with varying stability to crop diversification. We expect that these suggestions will move knowledge forward about biodiversity and ecosystem functioning in agroecosystems, and ultimately be of use to producers for optimizing soil health in their croplands.
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Native C4 grasses have become the preferred species for native perennial pastures and bioenergy production due to their high productivity under low soil nitrogen (N) status. One reason for their low N requirement is that C4 grasses may benefit from soil diazotrophs and promote biological N fixation. Our objective was to evaluate the impact of N fertilization rates (0, 67, and 202 kg N ha-1) and grass species (switchgrass [Panicum virgatum] and big bluestem [Andropogon gerardii]) on the abundance, activity, diversity, and community composition of soil diazotrophs over three agricultural seasons (grass green-up, initial harvest, and second harvest) in a field experiment in East Tennessee, United States. Nitrogen fertilization rate had a stronger influence on diazotroph population size and activity (determined by nifH gene and transcript abundances) and community composition (determined by nifH gene amplicon sequencing) than agricultural season or grass species. Excessive fertilization (202 kg N ha-1) resulted in fewer nifH transcripts compared to moderate fertilization (67 kg N ha-1) and decreased both richness and evenness of diazotrophic community, reflecting an inhibitory effect of high N application rates on soil diazotrophic community. Overall, cluster I and cluster III diazotrophs were dominant in this native C4 grass system. Diazotroph population size and activity were directly related to soil water content (SWC) based on structural equation modeling. Soil pH, SWC, and C and N availability were related to the variability of diazotrophic community composition. Our results revealed relationships between soil diazotrophic community and associated soil properties, adding to our understanding of the response of soil diazotrophs to N fertilization and grass species in native C4 grass systems.
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The combination of extended dry periods and high intensity rainfall, common in the southeastern US, leads to greater variability in soil moisture and consequently increases uncertainty to microbial processes pertinent to soil carbon (C) mineralization. However, field-based findings on soil moisture sensitivity to soil C cycling are very limited. Therefore, a field experiment was conducted in 2018 and 2019 on a soybean (Glycine max L.) cropland in the southeastern US with three soil moisture treatments: drought (simulated using rainout-shelter from June to October in each year), rainfed (natural precipitation), and irrigated (irrigation and precipitation). Soil respiration was measured weekly from May to November in both years. Soil samples were collected multiple times each year from 0-5, 5-15, and 15-30 cm depths to determine microbial biomass C (MBC), extractable organic C (EOC), hydrolytic enzyme activities, and fungal abundance. The cumulative respiration under drought compared to other treatments was lower by 32% to 33% in 2018 and 38% to 45% in 2019. Increased MBC, EOC, and fungal abundance were observed under drought than other treatments. Specific enzyme activity indicated fewer metabolically active microbes under drought treatment compared to rainfed and irrigated treatments. Also, maintenance of enzyme pool was observed under drought condition. These results provide critical insights on microbial metabolism in response to soil moisture variation and how that influences different pools of soil C under field conditions.
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Combined application of biochar and nitrogen (N) fertilizer has the potential to reduce N losses from soil. However, the effectiveness of biochar amendment on N management can vary with biochar types with different physical and chemical properties. This study aimed to assess the effect of two types of hardwood biochar with different ash contents and cation exchange capacity (CEC) on soil N mineralization and nitrous oxide (N2O) production when applied alone and in combination with N fertilizer. Soil samples collected from a temperate pasture system were amended with two types of biochar (B1 and B2), urea, and urea plus biochar, and incubated for 60 days along with soil control (without biochar or urea addition). Soil nitrate N, ammonium N, ammonia-oxidizing bacteria amoA gene transcripts, and N2O production were measured during the experiment. Compared to control, addition of B1 (higher CEC and lower ash content) alone decreased nitrate N concentration by 21% to 45% during the incubation period while the addition of B2 (lower CEC and higher ash content) alone increased the nitrate N concentration during the first 10 days. Biochar B1 also reduced the abundance of amoA transcripts by 71% after 60 days. Compared to B1 + urea, B2 + urea resulted in a significantly greater initial increase in soil ammonium and nitrate N concentrations. However, B2 + urea had a significantly lower 60-day cumulative N2O emission compared to B1 + urea. Overall, when applied with urea, the biochar with higher CEC reduced ammonification and nitrification rates, while biochar with higher ash content reduced N N2O production. Our study demonstrated that biochar has the potential to enhance N retention in soil and reduce N2O emission when it is applied with urea, but the specific effects of the added biochar depend on its physical and chemical properties.
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Carbón Orgánico , Fertilizantes/análisis , Nitrógeno/análisis , Suelo/química , AgriculturaRESUMEN
BACKGROUND: Fertilizer addition can contribute to nitrogen (N) losses from soil by affecting microbial populations responsible for nitrification. However, the effects of N fertilization on ammonia oxidizing bacteria under C4 perennial grasses in nutrient-poor grasslands are not well studied. METHODS: In this study, a field experiment was used to assess the effects of N fertilization rate (0, 67, and 202 kg N ha-1) and grass species (switchgrass (Panicum virgatum) and big bluestem (Andropogon gerardii)) on ammonia-oxidizing bacterial (AOB) communities in C4 grassland soils using quantitative PCR, quantitative reverse transcription-PCR, and high-throughput amplicon sequencing of amoA genes. RESULTS: Nitrosospira were dominant AOB in the C4 grassland soil throughout the growing season. N fertilization rate had a stronger influence on AOB community composition than C4 grass species. Elevated N fertilizer application increased the abundance, activity, and alpha-diversity of AOB communities as well as nitrification potential, nitrous oxide (N2O) emission and soil acidity. The abundance and species richness of AOB were higher under switchgrass compared to big bluestem. Soil pH, nitrate, nitrification potential, and N2O emission were significantly related to the variability in AOB community structures (p < 0.05).
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Crop residue retention and minimum tillage (including no-tillage, NT, and reduced tillage, RT) are common conservation tillage practices that have been extensively applied for improving soil health and reducing the negative environmental impact caused by intensive farming. However, the effects of minimum tillage, coupled with crop residue retention (including no-tillage plus residue retention, NTR, and reduced tillage plus residue retention, RTR), on soil organic carbon (SOC) stock have not been systematically analyzed. Using a dataset consisting of 1928 pairs of data points from 243 studies, we conducted a global meta-analysis to evaluate the effects of crop residue retention and minimum tillage on SOC stock in the 0-30 cm soil and how these effects varied with soil (soil sampling depth and texture), environmental (climate) and crop management conditions (cropping intensity), as well as treatment duration. We found that regardless of the climatic condition, crop management, or residue retention, minimum tillage alone increased the overall mean SOC stock. Specifically, NT and RT increased SOC stock by 11 and 6%, respectively, in comparison to conventional tillage (CT). Compared with CT, NTR and RTR increased SOC stock by 13 and 12%, respectively. The above effects were greater in the topsoil (62% of data points from the 0-15 cm depth) than in the subsoil (38% of data points from the 15-30 cm depth). Moreover, residue retention enhanced the resistance of SOC turnover to agricultural and environmental factors; mean annual temperature (coefficient = 0.15), soil pH (0.14), and experimental duration (0.08) were critical for increasing SOC stock with minimum tillage alone, while the response ratio of SOC stock under coupled residue retention and minimum tillage was insensitive to changes in those factors. Additionally, double cropping generally increased SOC stock cross all conservation tillage practices compared to multiple cropping. Therefore, we conclude that minimum tillage coupled with residue retention in a double-cropping system is the most promising management system for increasing SOC stocks in the 0-30 cm soil in croplands Our finding can inform sustainable soil management practices aimed at increasing resistance of SOC in croplands to climate change and soils degradation induced by intensive agriculture.
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Genetic engineering has been used to decrease the lignin content and to change the lignin composition of switchgrass (Panicum virgatum L.) to decrease cell wall recalcitrance to enable more efficient cellulosic biofuel production. Previous greenhouse and field studies showed that downregulation of the gene encoding switchgrass caffeic acid O-methyltransferase (COMT) and overexpression of the switchgrass PvMYB4 (MYB4) gene effectively improved ethanol yield. To understand potential environmental impacts of cultivating these transgenic bioenergy crops in the field, we quantified the effects of field cultivation of transgenic switchgrass on soil organic carbon (SOC) dynamics. Total and active SOC as well as soil respiration were measured in soils grown with two COMT-downregulated transgenic lines (COMT2 and COMT3), three MYB4-overexpressed transgenic lines (L1, L6, and L8), and their corresponding non-transgenic controls. No differences in total SOC, dissolved organic carbon (DOC), and permanganate oxidizable carbon (POXC) were detected between transgenic and non-transgenic treatments for both COMT (10.4-11.1 g kg-1 for SOC, 60.0-64.8 mg kg-1 for DOC, and 299-384 mg kg-1 for POXC) and MYB4 lines (6.89-8.21 g kg-1 for SOC, 56.0-61.1 mg kg-1 for DOC, and 177-199 mg kg-1 for POXC). Soil CO2-carbon (CO2-C) production from the COMT2 transgenic line was not significantly different from its non-transgenic control. In contrast, the COMT3 transgenic line had greater soil CO2-C production than its non-transgenic control (210 vs. 165 µg g-1) after 72 days of laboratory incubation. Combining the improvement in ethanol yield and biomass production reported in previous studies with negligible change in SOC and soil respiration, COMT2 could be a better biofuel feedstock than COMT3 for environmental conservation and cost-effective biofuel production. On the other hand, MYB4 transgenic line L8 produced more biomass and total ethanol per hectare while it released more CO2-C than the control (253 vs. 207 µg g-1). Long-term in situ monitoring of transgenic switchgrass systems using a suite of soil and environmental variables is needed to determine the sustainability of growing genetically modified bioenergy crops.
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Excessive crop residue returned to the soil hinders farm operations, but residue removal can affect soil quality. In contrast, cover cropping can return additional residue to the soil and improve soils and environmental quality compared with no cover cropping. Residue and cover crop impacts on soil surface greenhouses gas (GHG) emissions are undetermined and site specific. Thus, the present study was conducted to investigate the impacts of corn ( L.) residue management and cover cropping on GHG fluxes. The fluxes were measured from 2013 to 2015 using static chamber under corn and soybean [ (L.) Merr.] rotation initiated in 2000 at Brookings, SD. Treatments included two residue management levels (residue returned [RR] and residue not returned [RNR]) and two cover cropping (cover crops [CC] and no cover crops [NCC]). Results showed that RR under corn and soybean phases significantly reduced cumulative CO fluxes (2681.3 kg ha in corn and 2419.8 kg ha in soybeans) compared with RNR (3331.0 kg ha in corn and 2755.0 kg ha in soybeans) in 2013. The RR emitted significantly less cumulative NO fluxes than RNR from both the phases in 2013 and 2014, but not in 2015. The CC treatment had significantly lower cumulative NO fluxes than the NCC for corn and soybean phases in 2013 and 2014. We conclude that crop residue retention and cover cropping can mitigate the GHG emissions compared with residue removal and no cover cropping.
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Agricultura/métodos , Productos Agrícolas/crecimiento & desarrollo , Monitoreo del Ambiente , Gases de Efecto Invernadero/análisis , Dióxido de Carbono/análisis , Nitrógeno/análisis , Óxido Nitroso/análisis , Suelo , Glycine max , Zea maysRESUMEN
Reoxidation and mobilization of previously reduced and immobilized uranium by dissolved-phase oxidants poses a significant challenge for remediating uranium-contaminated groundwater. Preferential oxidation of reduced sulfur-bearing species, as opposed to reduced uranium-bearing species, has been demonstrated to limit the mobility of uranium at the laboratory scale yet field-scale investigations are lacking. In this study, the mobility of uranium in the presence of nitrate oxidant was investigated in a shallow groundwater system after establishing conditions conducive to uranium reduction and the formation of reduced sulfur-bearing species. A series of three injections of groundwater (200 L) containing U(VI) (5 µM) and amended with ethanol (40 mM) and sulfate (20 mM) were conducted in ten test wells in order to stimulate microbial-mediated reduction of uranium and the formation of reduced sulfur-bearing species. Simultaneous push-pull tests were then conducted in triplicate well clusters to investigate the mobility of U(VI) under three conditions: 1) high nitrate (120 mM), 2) high nitrate (120 mM) with ethanol (30 mM), and 3) low nitrate (2 mM) with ethanol (30 mM). Dilution-adjusted breakthrough curves of ethanol, nitrate, nitrite, sulfate, and U(VI) suggested that nitrate reduction was predominantly coupled to the oxidation of reduced-sulfur bearing species, as opposed to the reoxidation of U(IV), under all three conditions for the duration of the 36-day tests. The amount of sulfate, but not U(VI), recovered during the push-pull tests was substantially more than injected, relative to bromide tracer, under all three conditions and further suggested that reduced sulfur-bearing species were preferentially oxidized under nitrate-reducing conditions. However, some reoxidation of U(IV) was observed under nitrate-reducing conditions and in the absence of detectable nitrate and/or nitrite. This suggested that reduced sulfur-bearing species may not be fully effective at limiting the mobility of uranium in the presence of dissolved and/or solid-phase oxidants. The results of this field study confirmed those of previous laboratory studies which suggested that reoxidation of uranium under nitrate-reducing conditions can be substantially limited by preferential oxidation of reduced sulfur-bearing species.
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Nitratos/química , Uranio/análisis , Uranio/química , Contaminantes Químicos del Agua/análisis , Restauración y Remediación Ambiental , Etanol/química , Oxidación-Reducción , Sulfatos/química , Compuestos de Azufre/química , Tennessee , Contaminantes Químicos del Agua/químicaRESUMEN
Climate feedbacks from soils can result from environmental change followed by response of plant and microbial communities, and/or associated changes in nutrient cycling. Explicit consideration of microbial life-history traits and functions may be necessary to predict climate feedbacks owing to changes in the physiology and community composition of microbes and their associated effect on carbon cycling. Here we developed the microbial enzyme-mediated decomposition (MEND) model by incorporating microbial dormancy and the ability to track multiple isotopes of carbon. We tested two versions of MEND, that is, MEND with dormancy (MEND) and MEND without dormancy (MEND_wod), against long-term (270 days) carbon decomposition data from laboratory incubations of four soils with isotopically labeled substrates. MEND_wod adequately fitted multiple observations (total C-CO2 and (14)C-CO2 respiration, and dissolved organic carbon), but at the cost of significantly underestimating the total microbial biomass. MEND improved estimates of microbial biomass by 20-71% over MEND_wod. We also quantified uncertainties in parameters and model simulations using the Critical Objective Function Index method, which is based on a global stochastic optimization algorithm, as well as model complexity and observational data availability. Together our model extrapolations of the incubation study show that long-term soil incubations with experimental data for multiple carbon pools are conducive to estimate both decomposition and microbial parameters. These efforts should provide essential support to future field- and global-scale simulations, and enable more confident predictions of feedbacks between environmental change and carbon cycling.
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Carbono/química , Ecosistema , Modelos Biológicos , Microbiología del Suelo , Suelo/química , Humanos , Reproducibilidad de los ResultadosRESUMEN
The complexity of the mineral-organic carbon interface may influence the extent of stabilization of organic carbon compounds in soils, which is important for global climate futures. The nanoscale structure of a model interface was examined here by depositing films of organic carbon compounds of contrasting chemical character, hydrophilic glucose and amphiphilic stearic acid, onto a soil mineral analogue (Al2O3). Neutron reflectometry, a technique which provides depth-sensitive insight into the organization of the thin films, indicates that glucose molecules reside in a layer between Al2O3 and stearic acid, a result that was verified by water contact angle measurements. Molecular dynamics simulations reveal the thermodynamic driving force behind glucose partitioning on the mineral interface: The entropic penalty of confining the less mobile glucose on the mineral surface is lower than for stearic acid. The fundamental information obtained here helps rationalize how complex arrangements of organic carbon on soil mineral surfaces may arise.
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Óxido de Aluminio/química , Carbono/química , Suelo/química , Glucosa/química , Interacciones Hidrofóbicas e Hidrofílicas , Simulación de Dinámica Molecular , Ácidos Esteáricos/químicaRESUMEN
Enzyme dynamics are being incorporated into soil carbon cycling models and accurate representation of enzyme kinetics is an important step in predicting belowground nutrient dynamics. A scarce number of studies have measured activation energy (Ea) in soils and fewer studies have measured Ea in arctic and tropical soils, or in subsurface soils. We determined the Ea for four typical lignocellulose degrading enzymes in the A and B horizons of seven soils covering six different soil orders. We also elucidated which soil properties predicted any measurable differences in Ea. ß-glucosidase, cellobiohydrolase, phenol oxidase and peroxidase activities were measured at five temperatures, 4, 21, 30, 40, and 60°C. Ea was calculated using the Arrhenius equation. ß-glucosidase and cellobiohydrolase Ea values for both A and B horizons in this study were similar to previously reported values, however we could not make a direct comparison for B horizon soils because of the lack of data. There was no consistent relationship between hydrolase enzyme Ea and the environmental variables we measured. Phenol oxidase was the only enzyme that had a consistent positive relationship between Ea and pH in both horizons. The Ea in the arctic and subarctic zones for peroxidase was lower than the hydrolases and phenol oxidase values, indicating peroxidase may be a rate limited enzyme in environments under warming conditions. By including these six soil types we have increased the number of soil oxidative enzyme Ea values reported in the literature by 50%. This study is a step towards better quantifying enzyme kinetics in different climate zones.
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Enzimas/química , Microbiología del Suelo , Suelo/química , Ambiente , Hidrólisis , Oxidación-ReducciónRESUMEN
BACKGROUND: Physico-chemical sorption onto soil minerals is one of the major processes of dissolved organic carbon (OC) stabilization in deeper soils. The interaction of DOC on soil solids is related to the reactivity of soil minerals, the chemistry of sorbate functional groups, and the stability of sorbate to microbial degradation. This study was conducted to examine the sorption of diverse OC compounds (D-glucose, L-alanine, oxalic acid, salicylic acid, and sinapyl alcohol) on temperate climate soil orders (Mollisols, Ultisols and Alfisols). METHODOLOGY: Equilibrium batch experiments were conducted using 0-100 mg C L(-1) at a solid-solution ratio of 1â¶60 for 48 hrs on natural soils and on soils sterilized by γ-irradiation. The maximum sorption capacity, Q(max) and binding coefficient, k were calculated by fitting to the Langmuir model. RESULTS: Ultisols appeared to sorb more glucose, alanine, and salicylic acid than did Alfisols or Mollisols and the isotherms followed a non-linear pattern (higher k). Sterile experiments revealed that glucose and alanine were both readily degraded and/or incorporated into microbial biomass because the observed Q(max) under sterile conditions decreased by 22-46% for glucose and 17-77% for alanine as compared to non-sterile conditions. Mollisols, in contrast, more readily reacted with oxalic acid (Q(max) of 886 mg kg(-1)) and sinapyl alcohol (Q(max) of 2031 mg kg(-1)), and no degradation was observed. The reactivity of Alfisols to DOC was intermediate to that of Ultisols and Mollisols, and degradation followed similar patterns as for Ultisols. CONCLUSION: This study demonstrated that three common temperate soil orders experienced differential sorption and degradation of simple OC compounds, indicating that sorbate chemistry plays a significant role in the sorptive stabilization of DOC.