RESUMEN
Folding of the chromosomal fibre in interphase nuclei is an important element in the regulation of gene expression. For instance, physical contacts between promoters and enhancers are a key element in cell-type-specific transcription. We know remarkably little about the principles that control chromosome folding. Here we explore the view that intrachromosomal interactions, forming a complex pattern of loops, are a key element in chromosome folding. CTCF and cohesin are two abundant looping proteins of interphase chromosomes of higher eukaryotes. To investigate the role of looping in large-scale (supra Mb) folding of human chromosomes, we knocked down the gene that codes for CTCF and the one coding for Rad21, an essential subunit of cohesin. We measured the effect on chromosome folding using systematic 3D fluorescent in situ hybridization (FISH). Results show that chromatin becomes more compact after reducing the concentration of these two looping proteins. The molecular basis for this counter-intuitive behaviour is explored by polymer modelling usingy the Dynamic Loop model (Bohn M, Heermann DW (2010) Diffusion-driven looping provides a consistent framework for chromatin organization. PLoS ONE 5: e12218.). We show that compaction can be explained by selectively decreasing the number of short-range loops, leaving long-range looping unchanged. In support of this model prediction it has recently been shown by others that CTCF and cohesin indeed are responsible primarily for short-range looping. Our results suggest that the local and the overall changes in of chromosome structure are controlled by a delicate balance between short-range and long-range loops, allowing easy switching between, for instance, open and more compact chromatin states.
Asunto(s)
Cromatina/química , Cromatina/metabolismo , Modelos Moleculares , Proteínas Nucleares/química , Proteínas Nucleares/metabolismo , Fosfoproteínas/química , Fosfoproteínas/metabolismo , Proteínas Represoras/química , Proteínas Represoras/metabolismo , Apoptosis , Factor de Unión a CCCTC , Proteínas de Ciclo Celular , Células Cultivadas , Cromatina/genética , Proteínas Cromosómicas no Histona , Biología Computacional , Simulación por Computador , Proteínas de Unión al ADN , Técnicas de Silenciamiento del Gen , Humanos , Proteínas Nucleares/genética , Fosfoproteínas/genética , Polímeros , Proteínas Represoras/genética , CohesinasRESUMEN
To understand how interphase chromatin is organized in eukaryotic cell nuclei, it is essential to understand what kind of interactions influence the nuclear architecture and to what extent. Using a mesoscale model that incorporates chromatin-chromatin interactions as well as binding of chromatin to the nuclear envelope, we can show that chromatin loops and envelope bonds are major players in genome organization because they largely affect the entropy of the chromatin fibres. The model allows us to consistently reproduce multiple characteristic chromatin parameters in agreement with experimental data. We focus on the question of how an inversion of the nuclear architecture, in the course of which the highly active euchromatin changes its preferential position from the nuclear center to the periphery, can be achieved. We find that the transition between the common and inverted organization is driven by the strength of the envelope interaction and the nuclear chromatin density.
Asunto(s)
Eucromatina/metabolismo , Genoma Humano/fisiología , Modelos Biológicos , Membrana Nuclear/metabolismo , Animales , HumanosRESUMEN
Homologous recombination (HR) has a major impact in bacterial evolution. Most of the knowledge about the mechanisms and control of HR in bacteria has been obtained in fast growing bacteria. However, in their natural environment bacteria frequently meet adverse conditions which restrict the growth of cells. We have constructed a test system to investigate HR between a plasmid and a chromosome in carbon-starved populations of the soil bacterium Pseudomonas putida restoring the expression of phenol monooxygenase gene pheA. Our results show that prolonged starvation of P. putida in the presence of phenol stimulates HR. The emergence of recombinants on selective plates containing phenol as an only carbon source for the growth of recombinants is facilitated by reactive oxygen species and suppressed by DNA mismatch repair enzymes. Importantly, the chromosomal location of the HR target influences the frequency and dynamics of HR events. In silico analysis of binding sites of nucleoid-associated proteins (NAPs) revealed that chromosomal DNA regions which flank the test system in bacteria exhibiting a lower HR frequency are enriched in binding sites for a subset of NAPs compared to those which express a higher frequency of HR. We hypothesize that the binding of these proteins imposes differences in local structural organization of the genome that could affect the accessibility of the chromosomal DNA to HR processes and thereby the frequency of HR.
Asunto(s)
Carbono/metabolismo , Cromosomas Bacterianos , Recombinación Homóloga , Pseudomonas putida/genética , Reparación de la Incompatibilidad de ADN , Enzimas Reparadoras del ADN/genética , Estrés Oxidativo , Fenol/metabolismo , Especies Reactivas de Oxígeno/metabolismoRESUMEN
How do chromosomes fold and how does this depend on the organism and type of cell? This question has been addressed by a model and a methodology that uses only very basic constituents to capture the relevant features of folding. Key is the dynamic formation of loops within the chromosome. With this and entropy we show that the model is capable to describe the folding of human chromosomes in inter- and metaphase as well as for the Escherichia coli circular chromosomes.
Asunto(s)
Cromatina/genética , Cromosomas Humanos/genética , Modelos Genéticos , Algoritmos , Cromatina/ultraestructura , Cromosomas Bacterianos/genética , Escherichia coli/genética , Humanos , Modelos Moleculares , Conformación de Ácido Nucleico , TermodinámicaRESUMEN
Multiple studies suggest that chromatin looping might play a crucial role in organizing eukaryotic genomes. To investigate the interplay between the conformation of interphase chromatin and its transcriptional activity, we include information from gene expression profiles into a polymer model for chromatin that incorporates genomic loops. By relating loop formation to transcriptional activity, we are able to generate chromosome conformations whose structural and topological properties are consistent with experimental data. The model particularly allows to reproduce the conformational variations that are known to occur between highly and lowly expressed chromatin regions. As previously observed in experiments, lowly expressed regions of the simulated polymers are much more compact. Due to the changes in loop formation, the distributions of chromatin loops are also expression-dependent and exhibit a steeper decay in highly active regions. As a results of entropic interaction between differently looped parts of the chromosome, we observe topological alterations leading to a preferential positioning of highly transcribed loci closer to the surface of the chromosome territory. Considering the diffusional behavior of the chromatin fibre, the simulations furthermore show that the higher the expression level of specific parts of the chromatin fibre is, the more dynamic they are. The results exhibit that variations of loop formation along the chromatin fibre, and the entropic changes that come along with it, do not only influence the structural parameters on the local scale, but also effect the global chromosome conformation and topology.