RESUMEN
BACKGROUND: Complete fertilization failure after ICSI is a rare event, and it may happen repeatedly even in cases of normal sperm parameters and good ovarian response. In these cycles, alternative ICSI techniques may prove useful. METHODS: Our modified ICSI (mICSI) is characterized by aspiration close to the opposite membrane (the region of the mitochondria with a high inner mitochondrial membrane potential) which is followed by central deposition of the sperm. The method was applied prospectively to ICSI cycles of patients with a history of complete fertilization failure in previous ICSI cycles. In parallel, mICSI was compared with conventional ICSI in terms of further preimplantation development and treatment outcome. RESULTS: In patients with previous ICSI failures using conventional ICSI (no 2Pn zygotes out of 70 oocytes that had been injected) application of mICSI led to adequate fertilization (53.6%) and pregnancy rates (33.3%) (P < 0.001; P < 0.01). In patients without previous failed fertilization, no improvement in the rates of fertilization, blastocyst formation, implantation or clinical pregnancy could be seen. CONCLUSIONS: Our data indicate that the present version of ICSI is a reliable alternative to conventional ICSI. However, although it overcomes oocyte-dependent activation failure, routine application does not improve the overall results.
Asunto(s)
Microinyecciones/métodos , Oocitos/fisiología , Inyecciones de Esperma Intracitoplasmáticas/métodos , Adulto , Femenino , Fertilización , Humanos , Infertilidad Masculina , Masculino , Mitocondrias/fisiología , Embarazo , Índice de Embarazo , Estudios Prospectivos , Cigoto/fisiologíaRESUMEN
BACKGROUND: In MII oocytes showing difficult oolemma breakage, ICSI can cause an increase in the degeneration rate. This may be overcome by laser-assisted ICSI using a 5-10 micro m opening in the zona pellucida for injection. However, such a small opening might impair the hatching process, especially if assisted hatching is applied in addition. In order to prevent this, the present study used routine injection through an area of zona pellucida in which laser zona thinning had been applied, providing for both a reduced mechanical stress to the oocyte and assisted hatching. METHODS: This prospective study involved 100 cycles with 1016 MII oocytes. Conventional ICSI (control group) was compared with a modified laser-assisted ICSI (study group) in sibling oocytes. In the latter group oocytes were injected through an extended area of zona thinning. RESULTS: Degeneration rate was significantly lower in the study group (P < 0.004). There were no differences in fertilization, or formation and quality of blastocysts. In the study group embryo quality on day 2 was significantly better (P = 0.004) and herniation of day 5 blastocysts was increased (P = 0.005). Rates of implantation and pregnancy were not affected. However, on day 3 laser-assisted ICSI proved beneficial (P = 0.038) in terms of clinical pregnancy rate. CONCLUSIONS: The new method combines a less invasive ICSI technique with assisted hatching. Our preliminary data indicate that in addition to an improved oocyte survival, this new approach increases the hatching rate in vitro, which may explain the increase in pregnancy rate, at least in day 3 transfers.
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Rayos Láser , Inyecciones de Esperma Intracitoplasmáticas/métodos , Zona Pelúcida/efectos de la radiación , Adulto , Blastocisto/fisiología , Supervivencia Celular/efectos de la radiación , Implantación del Embrión , Embrión de Mamíferos/fisiología , Femenino , Humanos , Oocitos/fisiología , Embarazo , Índice de Embarazo , Resultado del TratamientoRESUMEN
Recent advances in ultrasound technology have enabled the diagnosis of overall tissue vascularization by three-dimensional (3D) power Doppler. This case report describes 3D power Doppler characteristics of unilateral ovarian torsion 2 weeks after embryo transfer in a pregnant patient with bilateral hyperstimulated ovaries. Before laparoscopic treatment the twisted right ovary showed the following 3D power Doppler indices: mean grayness index, 15.66; vacularization index, 0.24; flow index, 21.99; vascularization flow index, 0.05. One hour after laparoscopic treatment 3D power Doppler indices of the untwisted ovary were as follows: mean grayness index, 25.61; vacularization index, 3.81; flow index, 42.800; vascularization flow index, 1.63. The resistance index of the ovarian vessels before and after laparoscopy showed no significant difference (5.1 vs. 5.2). The diagnosis of ovarian torsion can be better made with 3D power Doppler sonography than with two-dimensional Doppler sonography.
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Transferencia de Embrión , Enfermedades del Ovario/diagnóstico por imagen , Complicaciones del Embarazo/diagnóstico por imagen , Dolor Abdominal/etiología , Adulto , Femenino , Humanos , Imagenología Tridimensional/métodos , Enfermedades del Ovario/etiología , Síndrome de Hiperestimulación Ovárica/etiología , Embarazo , Complicaciones del Embarazo/etiología , Anomalía Torsional/diagnóstico por imagen , Ultrasonografía Doppler en Color , Ultrasonografía Prenatal/métodosRESUMEN
AIM: This prospective study was performed to evaluate the predictive value of endometrium thickness in patients undergoing IVF-ET. METHOD: Measurements of endometrium thickness as well as pattern assessments were performed in 936 cycles (722 patients) on the day of administering human chronic gonadotropin (HCG). RESULTS: The overall pregnancy rate was 31.1 % (291/936). The age of non pregnant patient was significantly higher (p < 0.029). There was a significant influence of oestradiol (p < 0.029), number of transferred embryos (p < 0.004), and embryo quality (p < 0.002) on the pregnancy rate. Overall, the mean (+/- SD) endometrium thickness was 11.16 mm (+/- 2.13). The mean (+/- SD) endometrium thickness of pregnant patients was 11.25 mm (+/- 2.19). The mean (+/- SD) endometrium thickness of non-pregnant patients was 11.12 mm (+/- 2.10). A stepwise logistic regression analysis showed no statistically significant correlation between endometrium thickness and pregnancy rate (p < 0.23). CONCLUSION: Sonographic measurement of endometrium thickness on the day of human chorionic treatment with gonadotropin is not useful in predicting IVF outcome.
Asunto(s)
Endometrio/anatomía & histología , Endometrio/diagnóstico por imagen , Pruebas de Embarazo/métodos , Adulto , Transferencia de Embrión , Femenino , Humanos , Edad Materna , Valor Predictivo de las Pruebas , Embarazo , Embarazo de Alto Riesgo , UltrasonografíaRESUMEN
BACKGROUND: The hormonal milieu during ovarian stimulation is known to affect oolemma behaviour as well as zona pellucida thickness and structure. This led us to investigate whether a special subgroup of patients with oocytes where penetration of the oolemma is difficult during ICSI may benefit from assisted hatching. METHODS: A total of 77 couples (mean age: 32.9 +/- 4.6 years; range: 22-38) had oocytes that could hardly be penetrated by the ICSI pipette. Nineteen patients underwent two ICSI cycles, giving a total number of 96 cycles, which were randomly split into either the study group (n = 52) or the non-hatching group (n = 44). Hatching was done using a non-contact 1.48 mm wavelength diode laser. Implantation and pregnancy rates were recorded. RESULTS: The pregnancy rate was 36.6% (19/52) in the study group and 13.6% (6/44) in the non-hatching group (P < 0.05). In addition, a higher number (P < 0.05) of embryos implanted in the study group (23/106; 21.7%) than in the non-hatching group (9/92; 9.8%). CONCLUSIONS: Once oolema penetration during ICSI has proven difficult, prospective hatching of embryos considered for transfer may increase their implantation behaviour.
Asunto(s)
Implantación del Embrión , Embrión de Mamíferos/fisiología , Terapia por Láser , Técnicas Reproductivas Asistidas , Inyecciones de Esperma Intracitoplasmáticas , Zona Pelúcida/efectos de la radiación , Adulto , Femenino , Humanos , Masculino , Embarazo , Índice de Embarazo , Estudios Prospectivos , Zona Pelúcida/fisiologíaRESUMEN
OBJECTIVE: To evaluate the reproducibility of transvaginal three-dimensional (3D) endometrial volume measurement in patients with postmenopausal bleeding and to compare the reproducibility of this technique to that of two-dimensional (2D) endometrial thickness measurement. METHODS: In a prospective, blinded study, transvaginal ultrasound examinations were performed in 51 consecutive patients with postmenopausal bleeding. Three-dimensional volume and 2D thickness measurements were made and intraobserver and interobserver reproducibility of each technique were assessed. RESULTS: The intraobserver correlation of 3D endometrial volume measurement of the first observer was 0.97 and that of the second observer was 0.99. Thus, mean intraobserver correlation was 0.98. The mean interobserver correlation was 0.95 (0.95 vs. 0.96). There was no significant difference in reproducibility at different volume cut-offs. The mean intra- and interobserver correlation of endometrium volume measurements for five patients with endometrial carcinoma did not differ significantly from that for patients without carcinoma (0.98, 0.98 vs. 0.98, 0.95). The intraobserver correlation of 2D endometrial thickness measurements from the first observer was 0.71 and that from second observer 0.87. Thus, mean intraobserver correlation of the endometrial thickness measurements was 0.79. The mean interobserver correlation was 0.76 (0.84 vs. 0.70). CONCLUSIONS: Endometrial volume and thickness measurements by 3D and 2D ultrasound, respectively, show good reproducibility but the reproducibility of 3D ultrasound is better.
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Endometrio/diagnóstico por imagen , Ultrasonografía Doppler/métodos , Hemorragia Uterina/diagnóstico por imagen , Método Doble Ciego , Endometrio/patología , Femenino , Humanos , Persona de Mediana Edad , Posmenopausia , Estudios Prospectivos , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
PURPOSE: To determine the influence of technical pitfalls and oocyte morphology on survival rate and cleavage behavior after ICSI. METHODS: A total of 2210 injection procedures was examined for morphological and technical deviations. Survival rate and cleavage behavior were evaluated. RESULTS: In 77.8% of all cases ICSI was unsuspicous. Out of 491 deviations from optimal injection deep penetration of the oocyte and abundant presence of cumulus cells showed significant correlation with degeneration rate (p < 0.001). Morphological anomalies associated with the periphery of the oocyte were rather related to degeneration than cytoplasmic anomalies (p < 0.001). Early embryonic development was not impaired by technical or morphological parameters. CONCLUSIONS: To conclude, these prospective data may be of prognostic value in regard of the number of embryos available for transfer and may help to improve treatment outcome.