Posttranslational modifications of E2F family members in the physiological state and in cancer: Roles, mechanisms and therapeutic targets.

The E2F transcription factor family, whose members are encoded by the E2F1-E2F8 genes, plays pivotal roles in the cell cycle, apoptosis, metabolism, stemness, metastasis, aging, angiogenesis, tumor promotion or suppression, and other biological processes. The activity of E2Fs is regulated at multiple levels, with posttranslational modifications being an important regulatory mechanism. There are numerous types of posttranslational modifications, among which phosphorylation, acetylation, methylation, ubiquitination, SUMOylation, neddylation, and poly(ADP-ribosyl)ation are the most commonly studied in the context of the E2F family. Posttranslational modifications of E2F family proteins regulate their biological activity, stability, localization, and interactions with other biomolecules, affecting cell proliferation, apoptosis, DNA damage, etc., and thereby playing roles in physiological and pathological processes. Notably, these modifications do not always act alone but rather form an interactive regulatory network. Currently, several drugs targeting posttranslational modifications are being studied or clinically applied, in which the proteolysis-targeting chimera and molecular glue can target E2Fs. This review aims to summarize the roles and regulatory mechanisms of different PTMs of E2F family members in the physiological state and in cancer and to briefly discuss their clinical significance and potential therapeutic use.

Buried interface molecular hybrid for inverted perovskite solar cells.

Perovskite solar cells with an inverted architecture provide a key pathway for commercializing this emerging photovoltaic technology because of the better power conversion efficiency and operational stability compared with the normal device structure. Specifically, power conversion efficiencies of the inverted perovskite solar cells have exceeded 25% owing to the development of improved self-assembled molecules1-5 and passivation strategies6-8. However, poor wettability and agglomeration of self-assembled molecules9-12 cause interfacial losses, impeding further improvement in the power conversion efficiency and stability. Here we report a molecular hybrid at the buried interface in inverted perovskite solar cells that co-assembled the popular self-assembled molecule [4-(3,6-dimethyl-9H-carbazol-9-yl)butyl]phosphonic acid (Me-4PACz) with the multiple aromatic carboxylic acid 4,4',4″-nitrilotribenzoic acid (NA) to improve the heterojunction interface. The molecular hybrid of Me-4PACz with NA could substantially improve the interfacial characteristics. The resulting inverted perovskite solar cells demonstrated a record certified steady-state efficiency of 26.54%. Crucially, this strategy aligns seamlessly with large-scale manufacturing, achieving one of the highest certified power conversion efficiencies for inverted mini-modules at 22.74% (aperture area 11.1 cm2). Our device also maintained 96.1% of its initial power conversion efficiency after more than 2,400 h of 1-sun operation in ambient air.

In-Doped ZnO Electron Transport Layer for High-Efficiency Ultrathin Flexible Organic Solar Cells.

Sol-gel processed zinc oxide (ZnO) is one of the most widely used electron transport layers (ETLs) in inverted organic solar cells (OSCs). The high annealing temperature (≈200 °C) required for sintering to ensure a high electron mobility however results in severe damage to flexible substrates. Thus, flexible organic solar cells based on sol-gel processed ZnO exhibit significantly lower efficiency than rigid devices. In this paper, an indium-doping approach is developed to improve the optoelectronic properties of ZnO layers and reduce the required annealing temperature. Inverted OSCs based on In-doped ZnO (IZO) exhibit a higher efficiency than those based on ZnO for a range of different active layer systems. For the PM6:L8-BO system, the efficiency increases from 17.0% for the pristine ZnO-based device to 17.8% for the IZO-based device. The IZO-based device with an active layer of PM6:L8-BO:BTP-eC9 exhibits an even higher efficiency of up to 18.1%. In addition, a 1.2-micrometer-thick inverted ultrathin flexible organic solar cell is fabricated based on the IZO ETL that achieves an efficiency of 17.0% with a power-per-weight ratio of 40.4 W g-1, which is one of the highest efficiency for ultrathin (less than 10 micrometers) flexible organic solar cells.

Nitrogen-Blowing Assisted Strategy for Fabricating Large-Area Organic Solar Modules with an Efficiency of 15.6.

Organic solar cells (OSCs) are one of the most promising photovoltaic technologies due to their affordability and adaptability. However, upscaling is a critical issue that hinders the commercialization of OSCs. A significant challenge is the lack of cost-effective and facile techniques to modulate the morphology of the active layers. The slow solvent evaporation leads to an unfavorable phase separation, thus resulting in a low power conversion efficiency (PCE) of organic solar modules. Here, a nitrogen-blowing assisted method is developed to fabricate a large-area organic solar module (active area = 12 cm2) utilizing high-boiling-point solvents, achieving a PCE of 15.6%. The device fabricated with a high-boiling-point solvent produces a more uniform and smoother large-area film, and the assistance of nitrogen-blowing accelerates solvent evaporation, resulting in an optimized morphology with proper phase separation and finer aggregates. Moreover, the device fabricated by the nitrogen-blowing assisted method exhibits improved exciton dissociation, balanced carrier mobility, and reduced charge recombination. This work proposes a universal and cost-effective technique for the fabrication of high-efficiency organic solar modules.

Ecotoxicity and resistance genes induction changing of antibiotic tetracycline degradation products dominated by differential free radicals.

Studying the ecological risks of antibiotics and their degradation products is of great importance to water environment security and advanced oxidation processes (AOPs) development. This work studied the changes and internal influencing mechanisms of ecotoxicity and the capacity for inducing antibiotic resistance genes (ARGs) shown by the tetracycline (TC) degradation products generated in AOPs with differential free radicals. Under the action of superoxide radicals and singlet oxygen in the ozone system, and sulfate and hydroxyl radicals in the thermally activated potassium persulfate system, TC exhibited differential degradation pathways and resulted in the differential growth inhibition trends on the determined strains. Microcosm experiments combined with metagenomics were also performed to analyze the remarkable changes in the TC resistance genes tetA (60), tetT, and otr(B) induced by the degradation products and ARG hosts in the natural water environment. Microcosm experiments exhibited that the microbial community in actual water have changed significantly with the addition of TC and degradation intermediates. Furthermore, the richness of genes related to oxidative stress was investigated to discuss the effect on reactive oxygen species production and SOS response caused by TC and its intermediates.

Release of Zinc and Polycyclic Aromatic Hydrocarbons From Tire Crumb Rubber and Toxicity of Leachate to Daphnia magna: Effects of Tire Source and Photoaging.

Tire crumb rubber (TCR) has been widely used in artificial turf fields, however, the potential environmental risk of TCR and the effect of sunlight exposure are scarcely studied. Here, we evaluated leachability of Zn and polycyclic aromatic hydrocarbons (PAHs) in four types of TCRs and acute toxicity of leachates to Daphnia magna. The results showed that all types of TCRs tested released Zn (0.20-1.3 µg/g) and PAHs (9.4-17 µg/g) but only two were lethal to D. magna (mortality 73%). Notably, ultraviolet (UV) irradiation induced TCR to generate acidic leachate (pH ~ 4.8), which contained 24- and 1.2-fold higher concentrations of Zn and PAHs and therefore was more toxic to D. magna than that in the absence of UV treatment. These findings demonstrate source-dependent toxicity of TCR and highlight the need to consider the effect of photoaging when evaluating the environmental risks of TCR.