RESUMEN
A novel insulator string leakage current-monitoring sensor based on a fiber Bragg grating (FBG), cantilever beam, and spiral coil is demonstrated. The spiral coil is used to convert the leakage current into the force exerted on the cantilever structure, which is obtained by measuring the strain of the FBG. Experimental results show that the linear regression correlation coefficient is 0.99964. In addition, although the leakage current measurement is not sufficiently accurate, the 50-Hz component measurement is accurate. The sensor is suitable for use as an insulator string flashover warning and has promising prospects for future grid security protection applications.
RESUMEN
A Gram-stain-negative strain, 23-3-2T, was isolated from a nodule of Thermopsis lanceolate grown in Northwest China. Phylogenetic analysis of 16S rRNA gene sequence showed that the strain was closely related to Mesorhizobium camelthorni CCNWXJ 40-4T and M. alhagi CCNWXJ 12-2T having 98.0 and 97.9% similarities, respectively. Phylogenetic analysis based on the protein-coding genes atpD and glnA showed lower similarity with the same closely related species (94.5 and 89.9%, respectively), which suggest that 23-3-2T strain represents a distinctly delineated genospecies of the genus Mesorhizobium. The 23-3-2T strain grew at 20-37 °C temperature (optimum 28 °C) and 5.0-9.0 pH range (optimum pH 7.0). The cells contained Q-10 as the sole respiratory quinone and 18:1ω7c (24.56%) as the major cellular fatty acid. The DNA relatedness between the strain 23-3-2T and the two reference strains was 39-44%. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain 23-3-2T represents a novel species of the genus Mesorhizobium, for which the name Mesorhizobium zhangyense sp. nov. is proposed. The type strain is 23-3-2T (= CGMCC 1.15528T = NBRC 112337T). The respective DPD Taxon Number is TA00147.
Asunto(s)
Fabaceae/microbiología , Mesorhizobium/genética , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/análisis , Ácidos Grasos/química , Mesorhizobium/química , Mesorhizobium/aislamiento & purificación , Tipificación Molecular , Hibridación de Ácido Nucleico , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Ubiquinona/análogos & derivados , Ubiquinona/análisis , Ubiquinona/químicaRESUMEN
Corynebacterium glutamicum uses 4-hydroxybenzoic acid (4HBA) as sole carbon source for growth. Previous studies showed that 4HBA was taken up into cells via PcaK, and the aromatic ring was cleaved via protocatechuate 3,4-dioxygenase. In this study, the gene pobA ( Cg ) (ncgl1032) involved in the conversion of 4HBA into 3,4-dihydroxybenzoate (protocatechuate) was identified, and the gene product PobA (Cg) was characterized as a 4HBA 3-hydroxylase, which is a homodimer of PobA(Cg). The pobA (Cg) is physically associated with pcaK and formed a putative operon, but the two genes were located distantly to the pca cluster, which encode other enzymes for 4HBA/protocatechuate degradation. This new 4HBA 3-hydroxylase is unique in that it prefers NADPH to NADH as a cosubstrate, although its sequence is similar to other 4HBA 3-hydroxylases that prefer NADH as a cosubstrate. Sited-directed mutagenesis on putative NADPH-binding sites, D38 and T42, further improved its affinity to NADPH as well as its catalytic efficiency.
