Biochar leachate reduces primary nitrogen assimilation by inhibiting nitrogen fixation and microbial nitrate assimilation.

Biochar contains biotoxic aromatic compounds, and their influence on nitrogen-fixing cyanobacteria, the critical nitrogen fixer in paddy soil, has never been tested. Here, the physiological, metabolomic, and transcriptomic analyses of Nostoc sp. PCC7120 in response to biochar leachate were performed. The results suggested that biochar leachate inhibited the efficiency of photosynthesis, nitrogen fixation, and nitrate assimilation activities of nitrogen-fixing cyanobacteria. Biochar leachate containing aromatic compounds and odd- and long-chain saturated fatty acids impaired the membrane structure and antenna pigments, damaged the D1 protein of the oxygen evolution complex, and eventually decreased the electron transfer chain activity of photosystem II. Moreover, the nitrogen fixation and nitrate assimilation abilities of nitrogen-fixing cyanobacteria were inhibited by a decrease in photosynthetic productivity. A decrease in iron absorption was another factor limiting nitrogen fixation efficiency. Our study highlights that biochar with relatively high contents of dissolved organic matter poses a risk to primary nitrogen assimilation reduction and ecosystem nitrogen loss. Further evidence of the potential negative effects of biochar leachates on the fixation and assimilation capacity of nitrogen by soil microbes is needed to evaluate the impact of biochar on soil multifunctionality prior to large-scale application.

Mechanisms of total phosphorus removal and reduction of ß-lactam antibiotic resistance genes by exogenous fungal combination activated sludge.

This study utilized Trichoderma and activated sludge to construct combined activated sludge (TAS). The metagenomic approach was employed to examine the shifts in microbial community structure and function of TAS under amoxicillin stress and investigate the mechanism underlying the reduction of ß-lactam antibiotic resistance genes (ß-ARGs). The findings demonstrated that the elevated aundance of glpa, glpd, ugpq, glpq, and glpb were primarily responsible for the reduction in total phosphorus (TP) removal by TAS. The increased abundance of Proteobacteria and Verrucomicrobia led to enhanced expression of ugpb, phnd, and phne, thereby improving the TP removal of TAS. Furthermore, antibiotic inactivation has gradually become the primary antibiotic resistance mechanism in TAS. Specifically, an increase in the abundance of OXA-309 in TAS will decrease the probability of amoxicillin accumulation in TAS. A decrease in ß-ARGs diversity confirmed this. This study presents a novel approach to reducing antibiotic and ARG accumulation in sludge.

Biochar amendment reduces biological nitrogen fixation and nitrogen use efficiency in cadmium-contaminated paddy fields.

Cadmium (Cd) contamination poses a considerable threat to human health through grain enrichment and limits biological nitrogen fixation (BNF) in paddy fields. Biochar has shown great potential for agricultural soil remediation because it inactivates Cd, but uncertainties remain as to how biochar amendments affect BNF and grain N use efficiency in paddies. To elucidate these issues, we investigated the effects of biochar amendment on the structure and function of diazotrophic bacterial communities in different rice growth stages in Cd-contaminated paddy fields, and evaluated the contribution of BNF to grain N use efficiency under biochar amendment. The results showed that biochar amendment significantly increased the abundance of diazotrophic bacteria in the tillering and jointing stages. Furthermore, the community structure of soil diazotrophic bacteria markedly changed with biochar amendment, with a significant reduction in the abundances of Euryarchaeota, Desulfobacterales (Proteobacteria), and Sphingomonadales (Bacteroidetes) in the tillering stage. Changes in the soil carbon/nitrogen (C/N) ratio was the main factor driving diazotrophic microbial community characteristics caused by the release of available C from biochar at the tillering stage, rather than the Cd. Moreover, biochar amendment increased the efficiency of BNF (especially for autotrophic N2 fixation) in the vegetative phase of rice growth. Notably, biochar amendment significantly decreased BNF efficiency during the filling stage and reduced grain N use efficiency. The limited available nutrients in biochar and the toxicity of polycyclic aromatics and phenols in biochar-derived dissolved organic matter were responsible for the varied impacts of biochar on BNF in different rice growth stages. For the first time, we report that biochar amendment in paddy soils reduces Cd toxicity but also inhibits BNF and thereby decreases N use efficiency. Therefore, before applying biochar to inactivate Cd in paddy fields, there should be a trade-off between agricultural production and ecological safety to achieve sustainable agriculture.

Application of a newly recorded diazotrophic cyanobacterium in acidified and Cd contaminated paddy soil: Promotes rice yield and decreases Cd accumulation.

Acidification caused by excessive fertilization and heavy metals contamination are two prominent problems of agricultural soils. Diazotrophic cyanobacteria play important role in nitrogen (N) input in agricultural ecosystem. However, the effects of diazotrophic cyanobacteria on the growth of rice and heavy metal uptake by rice grain in acidified and heavy metal contaminated paddy soil remain unknown. In this study, a newly recorded diazotrophic cyanobacterium Aliinostoc sp. YYLX235 was isolated from acidified paddy soil. The results of pot experiment and in situ field plot experiment demonstrated that Aliinostoc sp. YYLX235 could promote rice grain yield and decrease cadmium (Cd) accumulation in rice grain. Nitrogen input by N2-fixation and increase of bio-available phosphorus (P) by promotion of activity of soil phosphatase may be the main mechanisms for growth-promoting effects of Aliinostoc sp. YYLX235 on rice. Binding and immobilization of Cd through hydroxyl, carboxyl, and amino groups may be the reason for decrease of Cd accumulation in rice grain by Aliinostoc sp. YYLX235 inoculation. The results presented in this study suggest that diazotrophic cyanobacteria have great potential in safe cropping in acidified and Cd contaminated paddy soils.

Coupling ammonia nitrogen adsorption and regeneration unit with a high-load anoxic/aerobic process to achieve rapid and efficient pollutants removal for wastewater treatment.

In this study, an ammonium nitrogen (NH4+-N) adsorption and regeneration (AAR) was constructed by a zeolite-packed column and NaClO-NaCl regeneration unit, and coupled with an anoxic/aerobic (AO) system to achieve efficient removal of carbon, nitrogen and phosphorus under short hydraulic retention time (HRT) and sludge retention time (SRT). Compared to conventional anaerobic/anoxic/aerobic (AAO) process, the proposed AO-AAR process achieved more efficient and stable nitrogen removal with greatly shorter HRT (5.6 h) and SRT (8 d) at 10.4 °C, with NH4+-N and total nitrogen in the effluent below 1.5 and 8.0 mg/L, respectively. The AO-AAR also obtained efficient phosphorus removal (<0.5 mg/L) by dosing aluminum in aerobic tank. High load and short SRT deteriorated sludge settleability and dewaterability, but enhanced methane production by improving sludge biodegradability. Dosing aluminum made the AO operating module more stable with improved settleability and dewaterability, and further enhanced methane production. Short HRT and SRT also resulted in the thriving of filamentous bacteria (Thiothrix) and heterotrophic nitrifiers (Acinetobacter, Pseudomonas and Rhodobacter) in the AO module, which helped in enhancing denitrification potential and nitrification efficiency under low temperature. Long-term operation showed that exchange capacity and physicochemical properties of zeolite were unchanged under NaClO-NaCl regeneration by introducing the tail gas from aerobic tank into the used regenerant to remove Ca2+ and Mg2+ exchanged from effluent of the AO module. Techno-economic analysis showed that the AO-AAR process is attractive and sustainable for municipal wastewater treatment by significantly improving nitrogen removal, greatly reducing land occupancy, enhancing methane production and achieving efficient reduction of carbon dioxide emission.