Preparation of limonin monoclonal antibody and establishment of a sensitive icELISA for analyzing limonin in citrus and herbal samples.

Limonin is an intensely bitter and highly oxidized tetracyclic triterpenoid secondary metabolite, which is abundant in the Rutaceae and Meliaceae, especially in Citrus. In order to detect limonin content in complex substrates such as citrus and traditional Chinese medicine, monoclonal antibodies specifically recognizing limonin were prepared and an indirect competitive enzyme-linked immunosorbent assay (icELISA) was established. The median inhibition concentration (IC50) was 5.40 ng/mL and the linear range was 1.25-23.84 ng/mL. The average recoveries from citrus peel and pulp samples were 95.9%-118.8% and 77.5%-113.1%, respectively. Moreover, the contents of limonin in 6 citrus samples and 4 herbal samples were analyzed by icELISA and UPLC-MS, and the results of the two methods were consistent. This validation is sufficient to demonstrate that the developed immunoassay is applicable for the detection of limonin in citrus and herbal samples and has the advantage of high efficiency, sensitivity, and convenience.

The Metabolism of Reactive Oxygen Species and Their Effects on Lipid Biosynthesis of Microalgae.

Microalgae have outstanding abilities to transform carbon dioxide (CO2) into useful lipids, which makes them extremely promising as renewable sources for manufacturing beneficial compounds. However, during this process, reactive oxygen species (ROS) can be inevitably formed via electron transfers in basal metabolisms. While the excessive accumulation of ROS can have negative effects, it has been supported that proper accumulation of ROS is essential to these organisms. Recent studies have shown that ROS increases are closely related to total lipid in microalgae under stress conditions. However, the exact mechanism behind this phenomenon remains largely unknown. Therefore, this paper aims to introduce the production and elimination of ROS in microalgae. The roles of ROS in three different signaling pathways for lipid biosynthesis are then reviewed: receptor proteins and phosphatases, as well as redox-sensitive transcription factors. Moreover, the strategies and applications of ROS-induced lipid biosynthesis in microalgae are summarized. Finally, future perspectives in this emerging field are also mentioned, appealing to more researchers to further explore the relative mechanisms. This may contribute to improving lipid accumulation in microalgae.

Measuring small-scale plasma irregularities in the high-latitude E- and F-regions simultaneously.

The ionosphere, Earth's space environment, exhibits widespread turbulent structuring, or plasma irregularities, visualized by the auroral displays seen in Earth's polar regions. Such plasma irregularities have been studied for decades, but plasma turbulence remains an elusive phenomenon. We combine scale-dependent measurements from a ground-based radar with satellite observations to characterize small-scale irregularities simultaneously in the bottomside and topside ionosphere and perform a statistical analysis on an aggregate from both instruments over time. We demonstrate the clear mapping of information vertically along the ionospheric altitude column, for field-perpendicular wavelengths down to 1.5 km. Our results paint a picture of the northern hemisphere high-latitude ionosphere as a turbulent system that is in a constant state of growth and decay; energy is being constantly injected and dissipated as the system is continuously attempting an accelerated return to equilibrium. We connect the widespread irregularity dissipation to Pedersen conductance in the E-region, and discuss the similarities between irregularities found in the polar cap and in the auroral region in that context. We find that the effects of a conducting E-region on certain turbulent properties (small-scale spectral index) is near ubiquitous in the dataset, and so we suggest that the electrodynamics of a conducting E-region must be considered when discussing plasma turbulence at high latitudes. This intimate relationship opens up the possibility that E-region conductivity is associated with the generation of F-region irregularities, though further studies are needed to assess that possibility.

Preparation of spirodiclofen monoclonal antibody and establishment of indirect competitive enzyme-linked immunosorbent assay.

Spirodiclofen, a spirocyclic tetronic acid derivative, has excellent acaricidal effect and is used worldwide to control the majority of important mite species. For monitoring its residue in food and environmental samples, two haptens containing different spacer arms were synthesized, a monoclonal antibody (mAb 5A4) against spirodiclofen was prepared, and a heterologous indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established. The 50% inhibition concentration (IC50) of ic-ELISA was 25.46 ng/mL, and the working range was 5.59-133.85 ng/mL. The ic-ELISA showed no cross-reactivity with structural analogs of spirodiclofen and other commonly-used acaricides. The average recoveries from Shiranui citrus samples and Yangtze River water were 85.62%-97.74% and 85.95%-99.30%, respectively. In the analysis of 12 citrus samples, the results of the ic-ELISA were quite similar to those of ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Hence, the new immunosorbent assay provides a substitute method for the qualitative and quantitative of spirodiclofen in food and environmental samples.

Three-channel metasurface based on simultaneous and independent control of near and far field under a single line light source.

Metasurface based on independent and simultaneous control of near field and far field has significant potential for use in multichannel optics platform devices. However, the previous studies cannot satisfy independent and simultaneous control of near field and far field under a single line source, which made a significant challenge to multichannel optical platforms working in a compact environment. To manipulate effectively and freely the amplitude and phase of transmission under line source, Marius' law and Propagation phase was introduced on all-dielectric encoding metasurfaces meta-atoms. The Marius' law and Propagation phase can control the size and rotation angle of meta-atoms to encode grayscale amplitude images and holographic phase images. Finite-difference time-domain simulation results reveal that dual channel metasurface under a single line source achieves the same display effect as the dual channel metasurface under multiple light sources, which proves the feasibility of our studies. Moreover, under different angles of the line source, we encode the near-field binary image by using the degeneracy rotation angle of meta-atoms. Finally, a three-channel metasurface was obtained without affecting the display of the previous two-channel metasurface. As a result, the independent control amplitude, phase, and polarization of the incident light wave were achieved. The proposed metasurface could be applied in creating a multi-channel metasurface optical platform in a compact environment, which has application potential in image displays, optical storage, optical anti-counterfeiting, and information encryption technology.

Interferometric Study of Ionospheric Plasma Irregularities in Regions of Phase Scintillations and HF Backscatter.

We investigate the nature of small-scale irregularities observed in the cusp by the Twin Rockets to Investigate Cusp Electrodynamics-2 (TRICE-2) in regions of enhanced phase scintillations and high-frequency coherent radar backscatter. We take advantage of the fact that the irregularities were detected by spatially separated probes, and present an interferometric analysis of both the observed electron density and electric field fluctuations. We provide evidence that fluctuations spanning a few decameters to about a meter have low phase velocity in the plasma reference frame and are nondispersive, confirming that decameter-scale irregularities follow the E × B velocity. Furthermore, we show that these "spatial" structures are intermittent and prominent outside of regions with strongest precipitation. The observations are then discussed in the context of possible mechanisms for irregularity creation.

Ionospheric Plasma IRregularities - IPIR - Data Product Based on Data From the Swarm Satellites.

Ionospheric plasma irregularities can be successfully studied with the Swarm satellites. Parameters derived from the in-situ plasma measurements and from the topside ionosphere total electron content provide a comprehensive dataset for characterizing plasma structuring along the orbits of the Swarm satellites. The Ionospheric Plasma IRregularities (IPIR) data product summarizes these parameters and allows for systematic studies of ionospheric irregularities. IPIR has already been used in investigations of structuring and variability of ionospheric plasma. This report provides a detailed description of algorithms behind the IPIR data product and demonstrates its use for ionospheric studies.

Human UFSP1 translated from an upstream near-cognate initiation codon functions as an active UFM1-specific protease.

Ubiquitin-fold modifier 1 (UFM1) is a recently identified ubiquitin-like posttranslational modification with important biological functions. However, the regulatory mechanisms governing UFM1 modification of target proteins (UFMylation) and the cellular processes controlled by UFMylation remain largely unknown. It has been previously shown that a UFM1-specific protease (UFSP2) mediates the maturation of the UFM1 precursor and drives the de-UFMylation reaction. Furthermore, it has long been thought that UFSP1, an ortholog of UFSP2, is inactive in many organisms, including human, because it lacks an apparent protease domain when translated from the canonical start codon (445AUG). Here, we demonstrate using the combination of site-directed mutagenesis, CRISPR/Cas9-mediated genome editing, and mass spectrometry approaches that translation of human UFSP1 initiates from an upstream near-cognate codon, 217CUG, via eukaryotic translation initiation factor eIF2A-mediated translational initiation rather than from the annotated 445AUG, revealing the presence of a catalytic protease domain containing a Cys active site. Moreover, we show that both UFSP1 and UFSP2 mediate maturation of UFM1 and de-UFMylation of target proteins. This study demonstrates that human UFSP1 functions as an active UFM1-specific protease, thus contributing to our understanding of the UFMylation/de-UFMylation process.

Hydroxyethyl cellulose sensitized SMDMS structure with optical fiber relative humidity and temperature simultaneous measurement sensor.

We have manufactured an intensity modulated optical fiber SMDMS sensor with hydroxyethyl cellulose (HEC) hydrogel coating for simultaneous measurement of RH and temperature. The SMDMS sensor was manufactured by splicing single-mode fiber (SMF), multi-mode fiber (MMF), dispersion compensation fiber (DCF), MMF, and SMF in sequence to form a structure of SMF + MMF + DCF + MMF + SMF (SMDMS). The cladding of MMFs and DCF were corroded by hydrofluoric acid (HF) and coated with HEC hydrogel to excite a strong evanescent field and increase the sensitivity of the SMDMS sensor. The adsorption of water molecules by HEC will cause a change in the effective refractive index of cladding mode, which will eventually change the intensity of the transmission spectrum. The experimental results indicate that the sensitivities are 0.507 dB/%RH and 0.345 dB/°C in the RH range of 30%-80% and temperature range of 10°C-50°C, respectively. At last, a dual-parameter measurement matrix is constructed based on the experimental results to achieve the simultaneous measurement of RH and temperature. The SMDMS sensor has the advantages of high sensitivity and good robustness, and has potential application prospects in daily life and other fields.

Optimized protocol to detect protein UFMylation in cells and in vitro via immunoblotting.

Ubiquitin-fold modifier 1 (UFM1) system is a recently identified ubiquitin-like modification with essential biological functions. Similar to ubiquitination, the covalent conjugation of UFM1 (UFMylation) to target proteins involves a three-step enzymatic cascade catalyzed sequentially by UFM1-activating enzyme 5 (UBA5, E1), UFM1-conjugating enzyme 1 (UFC1, E2), and UFM1-specific ligase 1 (UFL1, E3). Here, we provide an optimized protocol adapted to previously reported methods for detecting the UFMylation of target protein in human cells and in vitro assays, respectively, with high reliability and reproducibility. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).

Single core-offset Mach-Zehnder interferometer coated with PVA for simultaneous measurement of relative humidity and temperature.

A single core-offset Mach-Zehnder interferometer (MZI) coated with polyvinyl alcohol (PVA) for simultaneous measurement of relative humidity (RH) and temperature is proposed in this paper. The sensing structure is fabricated by splicing dispersion compensating fiber (DCF) and no-core fiber (NCF) and splicing two single-mode fibers (SMF) at both ends, where the core-offset is located at the splicing of SMF and DCF. A part of the cladding of DCF is etched to excite the high-order cladding mode (LP10), and PVA is coated on the etched area. The refractive index of PVA varies due to the adsorption of water molecules. Therefore, when the ambient relative humidity and temperature change, the change of MZI phase difference causes the wavelength of the resonant dip to shift. The experimental results indicate that the proposed sensor has a sensitivity of 0.256 nm/RH% for RH range of 30%-95%, and a sensitivity of 0.153 nm/℃ for temperature range of 20℃-80℃, respectively. The simultaneous measurement of RH and temperature can be achieved by demodulating the sensitivity coefficient matrix. The proposed sensor has the characteristics of good repeatability, high sensitivity, and good stability, which make it potentially applications for the detection of RH and temperature measurement.