Monitoring of sirolimus in the whole blood samples from pediatric patients with lymphatic anomalies.

In recent years, off-label use of sirolimus (SIR) has been gaining attention in the clinical practice. However, since it is critical to achieve and maintain therapeutic blood levels of SIR during treatment, the regular monitoring of this drug in individual patients must be implemented, especially in off-label indications of this drug. In this article, a fast, simple, and reliable analytical method for determining SIR levels in whole blood samples is proposed. Sample preparation based on dispersive liquid-liquid microextraction (DLLME) followed by liquid chromatography-mass spectrometry (LC-MS/MS) was fully optimized toward the analysis of SIR and proposed as a fast, simple, and reliable analytical method for determining the pharmacokinetic profile of SIR in whole-blood samples. In addition, the practical applicability of the proposed DLLME-LC-MS/MS method was evaluated by analyzing the pharmacokinetic profile of SIR in whole blood samples obtained from two pediatric patients suffering from lymphatic anomalies, receiving this drug as off-label clinical indication. The proposed methodology can be successfully applied in routine clinical practice for the fast and precise assessment of SIR levels in biological samples, thus allowing SIR dosages to be adjusted in real time during pharmacotherapy. Moreover, the measured SIR levels in the patients indicate the need for monitoring between doses to ensure the optimal pharmacotherapy of patients.

The critical evaluation of the effects of imidazolium-based ionic liquids on the separation efficiency of selected biogenic amines and their metabolites during MEKC analysis.

Ionic liquids (ILs) such as imidazole can be used to prevent the sorption of analytes onto the quartz walls of the capillary. Coating the capillary wall with a cation layer increases its surface stability, consequently improving the repeatability of separation process. Currently, examining the effects of dynamic coatings on the capillary wall is an emerging trend in capillary electrophoresis (CE) research. This study uses micellar electrokinetic chromatography (MEKC) to evaluate how ILs in the background electrolyte (BGE) affect the separation efficiency of biogenic amines (BAs). Specifically, this research focuses on 12 ILs built from cations containing an imidazole ring with different alkyl substituents and anions, as well as one IL containing a pyridinium cation with tetrafluoroborate anion. All analyzed ILs, which were added to the BGE in concentrations ranging from 1 to 20 mM, were tested for their ability to improve the electrophoretic separation of selected BAs, namely: homovanillic acid (HVA), vanililmandelic acid (VMA), dihydroxyphenylglicol (DHPG), 3-metoxy-4-hydroxyphenyl glicol (MHPG), normetanephrine (NM), metanephrine (M), and dihydroxyphenylacetic acid (DOPAC). The results showed that the most effective ILs added to the BGE were those with a chloride anion (1-hexyl-3-methylimidazolium chloride [HMIM+Cl-] and 1-ethyl-3-methylimidazolium chloride [EMIM+Cl-]) and those with a tetrafluoroborate anion (1-hexyl-3-methylimidazolium tetrafluoroborate [HMIM + BF4-]). Improved separation efficiency was also obtained for the BGE containing 1-hexyl-3-methylimidazolium hexafluorophosphate [HMIM + PF6-]. On the other hand, ILs with trifluoromethanesulfonate [OTf-] or bis(trifluoromethylsulfonyl)imide [NTf2-] anions, even at low concentrations in the BGE, disturbed the flow of current through the capillary and worsened the separation process. Overall, this study provides a critical evaluation of the impact of different types and concentrations of ILs on the performance of the MEKC method during the analysis of selected BAs.

Consumer behaviour during COVID-19 pandemic.

BACKGROUND: COVID-19 pandemic poses a significant threat in area of public health. In the light of dynamic increase of infections European countries decided to implement special safety measures in order to stop spread of SARS-CoV-2 virus. Reduction of the activity of shopping galleries, grocery stores and gastronomy directly impacted the change of consumer behaviours. OBJECTIVE: The aim of this research was the analysis of consumer behaviour during COVID-19 pandemic regarding the use of gastronomical services, grocery shopping and undertaken safety measures. MATERIAL AND METHODS: The main instrument was original survey conducted on-line in November 2020. The form consisted of metrics and close-ended questions about consumer behaviour during COVID-19 pandemic. 1080 respondents took part in the study (females - 850; men - 230). Results were mapped out in Statistics 13.1 program and graphical representation was prepared in Microsoft Excel. RESULTS: More than half of respondents were working people (n=629; 58%). Vast majority of working respondents during the study were working stationary (n=304; 48%), while remotely only 33% (n=208). Among young respondents almost half of the group was unemployed (n=158; 45%), while the rest of the group was employed (n=195; 55%). Professional activities in the group of respondents that were still learning was mostly stationary/ physic (n=57; 29%). During the pandemic the stationary form of working switched into remote in every third of respondent (n=307; 28%). CONCLUSIONS: Global pandemic of COVID-19 forced people to change their current behaviours - both consumer and social. Consumers are aware of risks borne by SARS-CoV-2 infection, which may be concluded from undertaken safety measures.