An atypical Bacillus anthracis infection in a bull-A potential occupational health hazard.

Bacillus anthracis infecting cattle is usually identified based on the typical symptom: sudden death. Bacillus anthracis causing atypical symptoms may remain undiagnosed and represent a potential occupational health hazard for, that is veterinarians and producers, butchers and tanners. In the year 2004, one case of sudden death in a dairy farm in southern Finland was diagnosed as bovine anthrax. Four years later 2008, an atypical case of anthrax was diagnosed in the same holding. The bull was taken to the Production Animal Hospital of the Faculty of Veterinary Medicine, University of Helsinki because of fever, loss of appetite and a symmetrically swollen scrotal sac. Penicillin treatment cured the fever but not the swollen scrotum. Before the intended therapeutic castration, a punctuate consisting of 10 ml fluid collected into a syringe from the scrotal sac was cultivated on blood agar at 37°C. After 24 hr, an almost pure culture of a completely non-hemolytic Bacillus cereus-like bacteria was obtained. The strain was identified as B. anthracis using Ba-specific primers by the Finnish Food Safety Authority (RUOKAVIRASTO). After the diagnosis, the bull was euthanized and destroyed, the personnel were treated with prophylactic antibiotics and the clinic was disinfected. In this particular case, treatment with water, Virkon S and lime seemed to be effective to eliminate endospores and vegetative cells since no relapses of anthrax have occurred in 10 years. This case is the last reported anthrax case in Finland.

Particle size characterization and the indoor-to-outdoor relationship of atmospheric aerosols in Helsinki.

OBJECTIVES: The influence of traffic and meteorological conditions on aerosol characteristics outdoors, the relationship between indoor and outdoor aerosol particles, and the pollutant transport indoors by means of a mechanical ventilation system were studied. METHODS: Indoor and outdoor concentrations of fine-particle numbers were measured during the summer (15 May--30 June 2000) in one office located in the basement of a building in Helsinki, Finland. The total number concentration was measured with a condensation particle counter, and the particle number size distribution (7-600 nm) was measured with a differential mobility particle sizer. The size distribution (0.3-25 microm) of the indoor particle numbers was periodically measured with a laser particle counter. RESULTS: Meteorological conditions, especially wind direction, had the greatest effect on the total number concentration and the size distribution of aerosol particles outdoors. The outdoor number concentration of ultrafine particles (diameter <100 nm) was strongly dependent on traffic density. The temporal variations in the indoor number concentration of ultrafine and fine particles (7-600 nm) closely followed the corresponding temporal variations outdoors. The building ventilation system was the main means of transporting aerosols indoors. The mean penetration factor was 0.41 (SD 0.11) for the nucleation mode (7-25 nm), 0.74 (SD 0.09) for the Aitken mode (25-100 nm), and 0.87 (SD 0.06) for the accumulation mode (100-600 nm). CONCLUSIONS: The ultrafine particles were bimodal with a nucleation mode (particle diameter <25 nm) and an Aitken mode (25 nm