Nucleic acid-responsive smart systems for controlled cargo delivery.

Stimulus-responsive delivery systems allow controlled, highly regulated, and efficient delivery of various cargos while minimizing side effects. Owing to the unique properties of nucleic acids, including the ability to adopt complex structures by base pairing, their easy synthesis, high specificity, shape memory, and configurability, they have been employed in autonomous molecular motors, logic circuits, reconfigurable nanoplatforms, and catalytic amplifiers. Moreover, the development of nucleic acid (NA)-responsive intelligent delivery vehicles is a rapidly growing field. These vehicles have attracted much attention in recent years due to their programmable, controllable, and reversible properties. In this work, we review several types of NA-responsive controlled delivery vehicles based on locks and keys, including DNA/RNA-responsive, aptamer-responsive, and CRISPR-responsive, and summarize their advantages and limitations.

Multilayered mucoadhesive hydrogel films based on Ocimum basilicum seed mucilage/thiolated alginate/dopamine-modified hyaluronic acid and PDA coating for sublingual administration of nystatin.

The present study establishes an experimental design for the preparation of new bi and tri-layer mucoadhesive sublingual films based on basil (Ocimum basilicum L.) seed mucilage (OBM) as novel plant-polysaccharide for oromucosal administration of nystatin (Nys). The films formulation consists of a drug reservoir-mucoadhesive layer cross-linked via CaCl2, with protective mucoadhesive layers based on thiolated alginate (TA) and polydopamine (PDA). OBM served as a new mucoadhesive polysaccharide in second layers, where the dopamine-modified-hyaluronic acid (DHA) improved the mucoadhesive strength and swelling rate properties. The drug-loaded formulations of trilayer film with PDA coating, and bilayer film with DHA/OBM (1:1) in the second layer, showed the desired mucoadhesion properties (about 69 and 75.3% respectively). The obtained results revealed that the bilayer film containing DHA had a superior swelling degree in the range of 15-19 (g/g). While the PDA coating sample showed the highest resistance to water uptake and erosion. The bilayer film (DHA/OBM with 1:1 ratio) provided a maximum drug release of 86% after 4 h. The selected formulations indicated good mechanical properties with no cytotoxicity.