RESUMEN
BACKGROUND: CD8+ T cells and natural killer (NK) cells are involved in the immune response against some pathogens. For this purpose, we investigated the in situ paracoccidioidomycosis (PCM) immune response addressing the participation of NK cells, CD8+ T cells, perforin and granzyme B expression. METHODS: Sixty biopsies of PCM skin and mucosa were classified according to the presence of compact granulomas (G1), poorly organized granulomas (G2) and both kinds in the same lesion (G3). CD8+ T cells, NK cells, perforin and granzyme B were showed by immunohistochemistry. RESULTS: CD8+ T cells were increased over NK cells in cutaneous G1 and G2 lesions. There was no difference regarding such cells in G3 lesions, although they were abundant in such lesions. In mucosa, CD8+ T cells were increased in number over NK cells in all groups. Granzyme B in skin increased in G2 and G3. The number of granzyme did not differ in mucosal lesions in the three groups. CONCLUSIONS: CD8+ T cells and NK cells play a role in PCM cutaneous and mucosal lesions. The predominance of CD8+ T cells over NK cells may represent an effective response against the fungi. Moreover, the high number of granzyme B expressing cells corroborates this possibility.
Asunto(s)
Citotoxicidad Inmunológica/inmunología , Membrana Mucosa/inmunología , Paracoccidioidomicosis/inmunología , Piel/inmunología , Linfocitos T CD8-positivos/inmunología , Linfocitos T CD8-positivos/metabolismo , Linfocitos T CD8-positivos/patología , Recuento de Células , Granzimas/metabolismo , Humanos , Inmunohistoquímica , Células Asesinas Naturales/inmunología , Células Asesinas Naturales/metabolismo , Células Asesinas Naturales/patología , Membrana Mucosa/metabolismo , Membrana Mucosa/microbiología , Membrana Mucosa/patología , Paracoccidioidomicosis/metabolismo , Paracoccidioidomicosis/patología , Perforina/metabolismo , Piel/metabolismo , Piel/microbiología , Piel/patología , Estadísticas no ParamétricasRESUMEN
In the disseminated form of histoplasmosis, isolation and further identification of Histoplasma capsulatum can be performed by several methods, namely, bone marrow aspiration, blood culture, and liver biopsy. Lymph node disease usually is diagnosed by excisional biopsy. Although fungal stains can identify this fungus, detection of specific antigens by immunohistochemistry shows a higher specificity and sensitivity. This approach can use the cell block method when the material is not sent to fungal cultures or fresh staining.
Asunto(s)
Biopsia con Aguja Fina , Histoplasmosis/diagnóstico , Linfadenitis/microbiología , Antígenos Fúngicos/análisis , Histoplasma/inmunología , Histoplasmosis/microbiología , Histoplasmosis/patología , Humanos , Técnicas para Inmunoenzimas , Masculino , Persona de Mediana Edad , CuelloRESUMEN
El aislamiento y la posterior identificación de Histoplasma capsulatum en lahistoplasmosis diseminada puede llevarse a cabo por diversos métodos,como la aspiración de médula ósea, el hemocultivo o la biopsia de hígado.La linfadenopatía es habitualmente diagnosticada por extirpación del ganglioafectado. Aunque la tinción del hongo puede llevar a su identificación,la detección de antígenos específicos mediante procedimientos deinmunohistoquímica muestra una mayor sensibilidad y especificidad.Este método permite la fijación de las células cuando el material no va a serprocesado para cultivo micológico o tinción en fresco(AU)
In the disseminated form of histoplasmosis, isolation and further identificationof Histoplasma capsulatum can be performed by several methods, namely,bone marrow aspiration, blood culture, and liver biopsy. Lymph node diseaseusually is diagnosed by excisional biopsy. Although fungal stains can identifythis fungus, detection of specific antigens by immunohistochemistry shows ahigher specificity and sensitivity. This approach can use the cell block methodwhen the material is not sent to fungal cultures or fresh staining(AU)