Chryseobacterium indolegenes infection in a patient with chronic obstructive pulmonary disease.

Chryseobacterium indolegenes is a rare pathogen that causes a variety of infections in inviduals who are mostly hospitalized with severe underlying diseases. Here we present a case of C. indolegenes in a 69-year-old male with chronic obstructive pulonary disease (COPD) who was admitted to the chest disease outpatient clinic with symptoms like cough, fever and sputum production and followed up on a suspicion of pneumonia. Despite the fact that our patient did not have any history of hospitalization for at least one year, pneumonia cause was due to C. indolegenes. Clinicians should pay attention to the rare pathogens such as C. indologenes while managing COPD patients without prior hospitalization history.

Investigation of intestinal parasites in dialysis patients.

OBJECTIVE: To search for the opportunistic and other pathogenic intestinal parasites in dialysis patients, and to compare the methods used for diagnosis. METHODS: This is a randomized study, which recruited participants from the dialysis patients. The study was carried out in the Department of Microbiology, Research Hospital, School of Medicine in Kocaeli University, Kocaeli, Turkey between June 2012 and March 2013. One hundred and forty-two patients were diagnosed with an end-stage renal failure, which underwent dialysis, and 150 healthy volunteers were enrolled in the study. Native-lugol, formol ethyl acetate sedimentation method, trichrome, modified trichrome, acid fast, and Calcofluor staining methods were applied to the stool samples. For the diagnosis of Cryptosporidium spp., Giardia intestinalis (G. intestinalis), and Entamoeba histolytica (E. histolytica), commercially available ELISA kits were used, which detect antigen in the stool. RESULTS: Parasites were found in 62 of the dialysis patients (43.7%) and 19 of the control group (12.7%). The most encountered parasitic agents in the dialysis patients were Blastocystis spp. (23.9%), G. intestinalis (8.5%), E. histolytica (2.1%), Microsporidia spp. (2.1%), and Cryptosporidium spp. (2.1%). The parasite detection rate of the formol ethyl acetate sedimentation method was found to be higher than native-lugol (p<0.05). CONCLUSION: To protect the dialysis patients with diarrhea from parasitic infections, it is important to carry out interval stool examinations with trichrome, modified trichrome, acid fast, and Calcofluor staining methods, and the ELISA method, which detects antigen in the stool.