Asunto(s)
Encéfalo/metabolismo , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/radioterapia , Riñón/metabolismo , Terapia por Luz de Baja Intensidad/métodos , Lisofosfatidilcolinas/metabolismo , Animales , Encéfalo/efectos de la radiación , Relación Dosis-Respuesta en la Radiación , Riñón/efectos de la radiación , Masculino , Especificidad de Órganos , Dosificación Radioterapéutica , RatasAsunto(s)
Epilepsia/metabolismo , Peroxidación de Lípido/fisiología , Pentilenotetrazol , Convulsiones/metabolismo , Animales , Sangre/metabolismo , Encéfalo/metabolismo , Epilepsia/inducido químicamente , Radicales Libres , Inyecciones Intramusculares , Hígado/metabolismo , Masculino , Neuronas/metabolismo , Ratas , Convulsiones/inducido químicamente , Vitamina E/farmacologíaAsunto(s)
Química Encefálica , Membrana Celular/química , Membrana Dobles de Lípidos/química , Hígado/química , Membranas Artificiales , Músculo Esquelético/química , Miocardio/química , Animales , Carpas , Conductividad Eléctrica , Impedancia Eléctrica , Femenino , Masculino , Especificidad de Órganos , Rana ridibunda , Ratas , Especificidad de la Especie , VertebradosAsunto(s)
Encéfalo/enzimología , Hígado/enzimología , Músculos/enzimología , Miocardio/enzimología , Superóxido Dismutasa/análisis , Vertebrados/metabolismo , Anfibios/metabolismo , Animales , Peces/metabolismo , Mamíferos/metabolismo , Reptiles/metabolismo , Superóxido Dismutasa/metabolismo , Distribución TisularAsunto(s)
Eritrocitos/fisiología , Halotano/toxicidad , Hemólisis/fisiología , Peroxidación de Lípido , Tiosulfatos/farmacología , Animales , Membrana Eritrocítica/efectos de los fármacos , Membrana Eritrocítica/fisiología , Eritrocitos/efectos de los fármacos , Hemólisis/efectos de los fármacos , Masculino , Ratas , Vitamina E/sangreAsunto(s)
Acetilcolina/farmacología , Encéfalo/metabolismo , Glicoesfingolípidos/metabolismo , Membranas Artificiales , Fosfolípidos/metabolismo , Animales , Bovinos , Glicoesfingolípidos/química , Técnicas In Vitro , Transporte Iónico/efectos de los fármacos , Membrana Dobles de Lípidos/química , Membrana Dobles de Lípidos/metabolismo , Modelos Biológicos , Permeabilidad , Fosfolípidos/química , TermodinámicaRESUMEN
The properties of DNA structure and the phospholipid content of Salmonella derby cells were studied with respect to their plasmid content and radiosensitivity. The role of R-plasmid in determining the qualitative and quantitative compositions of S. derby phospholipids was revealed. The radiosensitivity of plasmid-carrying S. derby mutants was shown to be most likely determined by the structure of DNA, its GC content, and the level of methylation. We suggest that the phospholipid molecules and their interaction with DNA play a key role in formation of the radio-resistance of plasmid-free S. derby cells.
Asunto(s)
ADN Bacteriano/química , Fosfolípidos/análisis , Plásmidos/química , Salmonella/química , Salmonella/genética , Metilación de ADN , ADN Bacteriano/aislamiento & purificación , Desnaturalización de Ácido Nucleico , Plásmidos/aislamiento & purificación , Factores R/química , Tolerancia a Radiación , Salmonella/efectos de la radiación , Especificidad de la Especie , TermodinámicaRESUMEN
The interaction between bovine adrenal medullary dopamine-beta-monooxygenase and liposomes from chromaffin granule membrane lipids as a function of pH, lipid and salt concentration was studied by ultracentrifugation. Efficient adsorption of dopamine-beta-monooxygenase to liposomes occurs in the pH range 5.0-6.5 and at low ionic strength. The adsorption was not detected in the case of apoenzyme. The membrane dopamine-beta-monooxygenase forms a complex with liposomes more effective than soluble does. The data obtained lead to certain conclusions about the specificity of complex between the enzyme and liposomes.
Asunto(s)
Médula Suprarrenal/metabolismo , Gránulos Cromafines/metabolismo , Dopamina beta-Hidroxilasa/metabolismo , Lípidos de la Membrana/metabolismo , Médula Suprarrenal/enzimología , Adsorción , Animales , Bovinos , Gránulos Cromafines/enzimología , Cinética , Liposomas , Concentración Osmolar , Unión ProteicaRESUMEN
The interaction of the membrane-bound and soluble forms of dopamine-beta-monooxygenase with a variety of lipid analogues of the membrane of chromaffin granules were studied. The use of two independent methods to determine the kinetics of product formation and of oxidation of the electron donor substrates (two substrates, dopamine and tyramine, and two electron donors, ascorbate and ferrocyanide), showed that the enzyme is activated in the presence of either phosphatidylcholine or lysophosphatidylcholine, but is inhibited by phosphatidylserine, phosphatidylethanolamine, and phosphatidic acid. The magnitude of the effects observed was the same for both forms of the enzyme. The parameters Vmax and Km for the substrates and for the electron donors were determined in the presence of each of the lipids tested. The data lead to certain conclusions about the mechanism of action of the lipids upon the enzyme and about the possible physiological role of the effects observed.
Asunto(s)
Dopamina beta-Hidroxilasa/metabolismo , Lípidos/farmacología , Animales , Catálisis/efectos de los fármacos , Bovinos , Gránulos Cromafines/enzimología , Dopamina beta-Hidroxilasa/antagonistas & inhibidores , Dopamina beta-Hidroxilasa/efectos de los fármacos , Activación Enzimática/efectos de los fármacos , Cinética , Proteínas de la Membrana/efectos de los fármacos , Proteínas de la Membrana/metabolismo , Oxidación-ReducciónRESUMEN
In brain and liver tissue of rats, treated repeatedly with alcohol, alterations in content of total phospholipids and polyphosphoinositols were reproducibly different. In the tissues studied content of polyphosphoinositols were distinctly decreased. Independent function of phosphoinositols in tissue metabolism as an auxiliary energy system in addition to the universal adenylate system is considered. These concepts are in agreement with observations on development of rough morphological alterations (fatty degeneration) in liver tissue. The fatty degeneration developed in the liver tissue in response to deficiency of oxidative potential in the organ.
