[The effect of vitamin D deficiency on the content of some soluble signaling molecules in the oral fluid in individuals with dental caries]. / Vliyanie nedostatka vitamina D v organizme na soderzhanie nekotorykh rastvorimykh signal'nykh molekul v rotovoi zhidkosti pri kariese zubov.

OBJECTIVE: The aim of the sthudy is to sthudy the level of soluble Immune Checkpoint Molecules (B7.2, CTLA-4, Tim-3, Lag-3, PD-1) in the oral fluid during dental caries with the background of a lack and/or deficiency of 25-hydroxy-vitamin D in body. MATERIALS AND METHODS: During the research 3 groups of people were formed, each one of them included 17 people aged from 20 to 24 years. The first group included students with high-intensity caries (above 9 DMFt index) and 25-hydroxy-vitamin D levels in blood serum >30 ng/ml, the second included students with high caries intensity and 25-hydroxy-vitamin D levels <30 ng/ml. The control group consisted of students with an average DMFt index of 1.5 (from 0 to 3) and a level of 25(OH)D in the blood more than 30 ng/ml. To determine the content of B7.2 (CD86), CTLA-4, Tim-3, Lag-3, PD-1, the Human Vascular Inflammation Panel 1 multiplex analysis kit from Biolegend (USA) was used. RESULTS: The results of the research showed that during dental caries with a normal level of 25-hydroxy-vitamin D there are no significant changes in the content of Immune Checkpoint Molecules. With the background of deficiency and lack of 25-hydroxy-vitamin D there is a decrease in the amount of B7.2, LAG-3, Tim-3 and PD-1. These changes are being aggravated with an increase of the caries intensity. CONCLUSION: Vitamin D deficiency leads to a decrease in mucosal immunity of the oral cavity, the multiplication of pathogenic microorganisms, which in turn, releasing various metabolites, including cytokine-like substances, aggravate the pathological process and intensify carious lesions.

Features of the content of matrix metalloproteinases ММР-9 and ММР-2 in mixed saliva of young individuals with dental caries against the background of different level of 25(OH) vitamin D in the body.

The content ofММР-9 and ММР-2 in oral fluid of 105 individuals between the ages of 19 and 23 has been researched.Of these, 42 people are individuals with dental caries and normal level of the active form of vitamin Din serum (25(OH)D >30ng/mL) and 42 people - with 25(OH)D <30 ng/mL level.The control group was composed of 21 individuals with low DMFt index (1,5) and a normal level of 25(OH)D in blood. It has been established that the level of ММР-9 in mixed salivaincreases against the background of dental caries,while the content of ММР-9 and ММР-2 increasessignificantlyamidthe lack and deficiency of25(OH)Din the body. Inverse correlations between the 25(OH)D level in serum and the value ofmatrix metalloproteinasesin saliva have been revealed: noticeable - with the amount of MMP-9 and moderate- with the concentration of MMP-2.

[Long-chain fatty acids and short-chain fatty acids in exhaled breath condensate of patients with chronic obstructive pulmonary disease]. / Osobennosti spektra korotkotsepochechnykh i vysshikh zhirnykh kislot v ékspirate u bol'nykh khronicheskoi obstruktivnoi bolezn'iu legkikh.

In present study we performed gas-liquid chromatographic analysis of exhaled breath condensate to measure volatile fatty acids (C2 - acetic, C3 - propionic, C4 - butanoic, isoC4 - isobutyric, C5 - valerianic, C6 - caproic, C7 - heptanoic) and fatty acid with a long aliphatic chain (C14:0 - myristic, C15:0 - pentadecanoic, C16:0 - palmitic, C16:1 - palmitooleic, C17:0 - heptadecanoic, C17:1 - heptadecenoic, C18:0 - stearic, C18:1 - oleic, C18:2 - linolenic, C18:3ω3 - α-linolenic, C20:4ω6 - arachidonic) in patients suffering from moderate chronic obstructive pulmonary disease (2nd stage, GOLD). We revealed the increase of the total amount of short chain fatty acids (C2, C3, C4, C5) and polyunsaturated (C18:2, C20:4ω6) fatty acids, meanwhile the level of saturated fatty acids (C16:0, C17:0, C18:0) decreased.

[Dynamics of immune and biochemical features of oral fluid in persons with caries receiving vitamin D]. / Dinamika nekotorykh immunnykh i biokhimicheskikh pokazatelei rotovoi zhidkosti u lits s kariesom na fone priema preparata vitamina D.

OBJECTIVE: The aim of the study was to evaluate the content of the vitamin D metabolite (25(OH)D3) in the blood serum, the level of some indicators of the immune and peroxidic status in the oral fluid in people with carious processes of different intensities while taking the native solution of vitamin D (international non-proprietary name Cholecalciferol). MATERIAL AND METHODS: The study included 47 people aged 20 to 22 with caries and low levels of 25(OH)D3 in blood serum. The control group consisted of 13 people with DMFT index of 0.00 [0.00; 0.00] and a normal serum content of 25(OH)D3. The amount of antimicrobial peptide cathelicidin LL-37, secretory immunoglobulin A, lipopolysaccharide binding protein (LBP) was determined in the oral fluid using ELISA reagent kits, and the content of intermediate lipid peroxidation products such as malondialdehyde (TBA-active products) and the level of total antioxidant activity were spectrophotometrically evaluated. RESULTS: Reduced serum concentrations of 25(OH)D3 were recorded predominantly in individuals with a DMFT index of more than 9.0. In patients with caries, at a concentration of 25(OH)D3 in blood serum below 30 ng/ml, a decrease in the values of secretory immunoglobulin A, lipopolysaccharide-binding protein, cathelicidin, and the level of total antioxidant activity was observed, accompanied by an increase in the number of intermediate products of lyoperoxidation in the oral fluid. The course intake of the native AquaDtrim vitamin D solution (Cholecalciferol) led to normalization of the values of certain immune and biochemical parameters (cathelicidin LL-37, secretory immunoglobulin A, LBP of the oral fluid) and restoration of balance in the system «lipid peroxidation - antioxidants¼. CONCLUSION: The results indicate the advisability of assessing the level of 25(OH)D3 in the body for the diagnosis of pathological processes in the oral cavity and the use of vitamin D preparations in the prevention and complex therapy of carious process.

[Clinical and some biochemical features of rhinosinusitis of different etiology]. / Klinicheskie i nekotorye biokhimicheskie osobennosti rinosinusitov razlichnoi étiologii.

Identified pathogens, and studied the features of the clinical picture, the level of antioxidant activity, the number of TBA-active products, cytokines, chaperones proteins and antibodies against them in the serum of patients with fungal, viral, bacterial rhinosinusitis (RS) were studied. Most RS of viral nature are caused by rhinoviruses, in turn, the causative agent of bacterial RS, most often acts as a polyflora (S. epidermidis, S. aureus, Pseudomonas aeruginosa). In the group of fungal RS prevailed A. fumigatus. It is noted that a more severe clinical picture in patients bacterial RS; it was manifested by a prolonged course, more pronounced nasal congestion, smell impairment and frequent relapses of the disease. High levels of LPO and a decrease in serum antioxidant activity were established for all studied infectious RS with a maximum imbalance in the LPO-AOP system in fungal forms; shift of the cytokine profile towards pro-inflammatory factors (IL-1b, IL-2, IL-8, IL-6) was registered in bacterial and viral RS and towards the values of anti-inflammatory IL-4 - in fungal RS. The increased amount of chaperone proteins was seen in bacterial RS; high concentration of antibodies against them was noted in fungal and viral RS.

[Determination of some indicators of immunity and lipoperoxidation in the oral fluid in persons with a low vitamin D level.]

Oral fluid is a unique biological environment, containing a wide range of substances, coming from local and systemic sources, which makes it possible to use it as an object for assessing pathological changes in the body both at the local and systemic levels. In comparison with the traditional method of blood analysis, the advantage of evaluating the parameters of the oral fluid is the non-invasive of this method of obtaining material. All patients underwent oral fluid sampling using special plastic containers with a swab, which facilitate the selection of material, eliminating the penetration of mucin into a clean test sample, which helps to obtain more accurate analysis results. The amount of secretory IgA, lipopolysaccharide-binding protein (LBP), TBA-active products, the level of total antioxidant activity in the oral fluid in individuals with a low level of 25(OH)D before and after taking the native solution of vitamin D "Aqua Trim" were determined. The concentrations of secretory immunoglobulin A, lipopolysaccharide, binding protein and the level of total antioxidant activity are reduced in the oral fluid of people with vitamin D deficiency, but the number of intermediate products of lyoperoxidation increases. The course intake of the native solution of vitamin D (International Nonproprietary Name - Colecalciferol) normalizes the functioning of the immunity of the oral cavity and restores the balance of the "lipid peroxidation-antioxidants" system.

[The prognostic significance of particular immunological indicators of lachrymal fluid in patients using soft contact lenses].

The study was carried out to identify concentrations of vessel epithelium growth factor (VEGF), souble forms of VEGF receptor type I and II, chemo-toxic protein I and metalloproteinase-9 in lachrymal fluid. The immune enzyme analysis was applied. He sampling included patients with myopia without complications while using silicone hydro-helium and hydro-helium contact lenses and patients with hypoxic complications of contact correction. It is established that application of hydro-helium and silicone hydro-helium contact lenses more than one year is followed by increasing of concentration of detected substances in lachrymal fluid. Under hypoxic complications of contact correction the levels of substances are significantly higher than in cases without complications. This occurrence makes it possible to substantiate possibility of application of immunologic indicators as diagnostic markers.

[The pathogenic aspects of fat acids metabolism with short chain and production of cytokines in target affected areas of skin under psoriasis].

The article deals with the results of analysis of specters of short-chained fat acids and cytokines in affected skin of patients with psoriasis. The study revealed the significant decrease of short-chained fat acids level, the shift of cytokine profile in the direction of anti-inflammatory factors (interleukins 1L-1beta, IL-8, tumor necrosis factor TNF-alpha, interferon IFN-alpha) and mytogenetic factors (EGF) on the background of stable values of anti-inflammatory cytokine IL-4. The direct pathogenically significant correlation relationships are established between the IL-4 level and the amount of most analyzed short-chained fat acids. The negative correlation relationships were established between content of C2 and IL-1beta.

[Variability of quantitative changes in short-chain fatty acids in serum and epidermis in psoriasis].

Gas liquid chromatography was used to study the spectrum of short-chain fatty acids (SCFA) in the serum and epidermal corneal layers of abnormal and apparently healthy skin areas in patients with psoriatic disease on an exacerbation and at a progressive stage. Significantly lower SCFA levels were detected in both psoriatic and intact areas, with derangements being mostly manifested in the papulae. There were heterodirectional serum volatile fatty acid spectrum changes that were characterized by a rise in the concentrations of isobutyrate, valeriate, and capronate and by a decrease in the proportion of acetate and propionate.