Embryonic thermal manipulation of poultry birds: Lucrative and deleterious effects.

The major efforts to improve feed conversion, increase the body weight and breast muscle yield of broilers have been focused on feeding and management at the post hatch period. However, incubation temperature is the most significant factor for the egg hatching rate, chick quality, and post hatch performance. Therefore, incubation factors affecting the performance should be taken with necessary precautions. Incubation temperature not only affects the early development of the hatchlings but also has a lasting impact on the characteristics of the chicks, such as final body weight and meat quality traits. This article provides an overview about embryonic thermal manipulation (TM) of domestic fowls and review the lucrative and deleterious effects of embryonic TM on embryo development, muscle growth, thermotolerance acquisition, and immunity.

Embryonic thermal manipulation of Japanese quail: effects on embryonic development, hatchability, and post-hatch performance.

Embryonic thermal manipulation led to several modifications in molecular, physiological, and biochemical parameters which affect pre- and post-hatch growth performance. The current study aims to elucidate the onset and long-term effects of intermittent thermal manipulations (TM) during two-time windows, early/late, of embryogenesis in Japanese quail (Coturnix japonica) on embryonic development, hatchability, muscle histogenesis, and post-hatch growth performance. Four groups were created; quail eggs in the control group were incubated at 37.7 °C and relative humidity (RH) 55%. Three thermally treated groups were incubated intermittently at 41 °C and 65% RH intermittently (3 h/day): early embryogenesis group (EE) was thermally treated during embryonic days (ED) 6-8, late embryogenesis group (LE) was thermally treated during (ED12-ED14), and early and late embryogenesis group (EL) was thermally manipulated in both time windows. Relative embryo weights in EL and EE were significantly lighter than those in LE and Ctrl groups. The hatched chicks were reared under optimal managemental conditions (three replicates per treatment). Average daily feed intake was recorded, and feed conversion ratio (FCR) was calculated. Histological and quantitative analyses of muscle fibers were performed. The results revealed that TM led to significant hypertrophy of quail breast muscle in (EE). Intermittent short-term (3-6 h) thermal manipulation (39-40 °C) protocols during early embryogenesis (ED6-ED8) could be recommended to enhance muscle mass growth and breast muscle yield in the Japanese quail.

Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System.

The myostatin (MSTN) gene is important because of its role in regulation of skeletal muscle growth in all vertebrates. In this study, CRISPR/Cas9 was utilized to successfully target the channel catfish, Ictalurus punctatus, muscle suppressor gene MSTN. CRISPR/Cas9 induced high rates (88-100%) of mutagenesis in the target protein-encoding sites of MSTN. MSTN-edited fry had more muscle cells (p < 0.001) than controls, and the mean body weight of gene-edited fry increased by 29.7%. The nucleic acid alignment of the mutated sequences against the wild-type sequence revealed multiple insertions and deletions. These results demonstrate that CRISPR/Cas9 is a highly efficient tool for editing the channel catfish genome, and opens ways for facilitating channel catfish genetic enhancement and functional genomics. This approach may produce growth-enhanced channel catfish and increase productivity.