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Spectrochim Acta A Mol Biomol Spectrosc
; 278: 121333, 2022 Oct 05.
Article
En
| MEDLINE
| ID: mdl-35537263
A probe with an isothiocyanate group was synthesized and evaluated for its H2S sensing ability. Upon addition of H2S, the probe exhibited ratiometric properties during absorption with a red-shift. The probe exhibited fluorescent off-on responses towards H2S via the ESIPT process, due to the conversion of isocyanate into amine. UV-vis, fluorescence, and 1H NMR spectroscopic analyses were performed to investigate the sensing mechanism. The probe has a large Stokes shift, short response time, and low detection limit. It can be used to estimate H2S levels within the range of 0-36 nM. The practical applicability of the probe was demonstrated using water samples and living cells.
Fluorescent Dyes , Hydrogen Sulfide , Fluorescent Dyes/chemistry , HeLa Cells , Humans , Hydrogen Sulfide/analysis , Isothiocyanates , Spectrometry, Fluorescence