[The outlook for the creation of a bank of cryopreserved cord blood for clinical use]. / Perspektiva sozdaniia banka kriokonservirovannoi kordovoi krovi dlia klinicheskogo primeneniia.

Appraisal of morphological and functional features of cord blood cells involving identification of precursor cells marked with specific monoclonal antibodies makes it possible to regard cord blood as biologically valuable hemopoietic tissue with a high repopulation potential. Studies aimed at developing optimal conditions of cryopreservation allow the stocks of biologically valuable tissue to be built up.

[Preclinical trials of polyplatillen, the antitumor activity of a complex platinum compound with DNA on a leukemia P388 model in a HEp-2 cell culture (1)]. / Doklinicheskie ispytaniia poliplatillena, protivoopukholevaia aktivnost' kompleksnogo soedineniia platiny s DNK na modeli leikemii P-388 v kul'ture kletok HEp-2 (soobshchenie 1).

The authors studied the antitumour activity of a new complex platinum compound (DDP-DNA) in mice with leukemia P-388 and in cell culture. DDP-DNA was introduced intraperitoneally 24 hours after inoculation of 2 x 10(6) tumour cells. It was established that by its antitumour activity DDP-DNA was not inferior then DDP. The antiproliferative activity of nontoxic doses of the preparation in cell culture has been shown.

[Study of tissue coagulation factors of native and cryopreserved bone marrow]. / Izuchenie tkanevykh faktorov svertyvaniia nativnogo i konservirovannogo kostnogo mozga.

Status of thromboplastin, antiheparin and antithrombin characteristics of the native and cryopreserved bone marrow was studied experimentally on 12 rabbits. It is found that both the native and cryopreserved bone marrow possesses a pronounced and dilution resistant thromboplastin activity, whereas antithrombin and heparin activities were statistically unauthentic. In that connection the bone marrow injected into blood circulation may have different effects depending on the initial state of the recipient blood coagulation. The fact should be taken into account in clinics.

[Cryopreservation of a suspension of fetal liver cells for clinical use]. / Kriokonservirovanie suspenzii kletok fetal'noi pecheni dlia klinicheskogo primeneniia.

The authors evaluate a method of procuring and preserving fetal liver cells in a medium containing low-molecular polyvynilpyrrolidon, glucose, lactose, sodium phosphate and sodiumhydrocitrate not requiring washing off. The biomaterial is stored at a temperature of -196 degrees C. The efficiency of this method of cryopreservation of fetal liver cell suspensions was confirmed by high morphological intactness, functional activity and proliferative capacity. Patients with hypoplastic anemia subjected to transfusion of cryopreserved fetal liver cells showed a normalization of bone marrow hemopoiesis, remissions.

[Relation of atherogenesis and T-immunodeficiency]. / Vzaimosvia'z aterogeneza i T-immunodefitsita.

Experiments on rabbits have shown that state of T-immunodeficiency induced by persisting virus infection promotes atherogenesis. Correction of age immunodeficiency by transplantation of the autologous bone marrow taken in young age considerably retards the atherosclerosis development. Inhibition of atherogenesis is also achieved by introduction (to animals) of natural thymic vilosene preparation which compensates a decrease in the functional activity of the thymus gland occurring under conditions of experimental hyperlipidemia.