A novel 2-(2-aminophenyl) imidazo [1,5-a] pyridine-based fluorescent probe for rapid detection of phosgene.

Phosgene is a highly concealed and highly toxic gas that seriously threatens human health and public security. Therefore, the detection of phosgene is of great significance to world security. Herein, a new type of fluorescent probe based on 2-(2-aminophenyl) imidazo [1,5-a] pyridine is reported for the rapid detection of phosgene. The probe itself only emits a faint green fluorescence, while phosgene allows it to produce a strong blue fluorescence. During the recognition process, phosgene interacts simultaneously with both amino site and imidazole moiety in the probe molecule, resulting in a four-ring-fused rigid structure with high fluorescence quantum yield. The probe not only has the characteristics of high efficiency, high sensitivity (detection limit 2.68 nM), and high selectivity, but also has remarkable spectral changes. Finally, a portable test strip is used to detect phosgene in the gas phase, and the fluorescent color change of the test strip can be easily observed. The most exciting thing is that the portable test strip with the probe PMPY-NH2 can produce a strong fluorescence response to 1 ppm of phosgene, which is far lower than the level of phosgene that seriously threatens to human health.

A facile dual-function fluorescent probe for detection of phosgene and nitrite and its applications in portable chemosensor analysis and food analysis.

Due to the potential threats of phosgene and nitrite to public health and safety, in this work, we first proposed the application of a facile dual-function fluorescent probe 2-(1H-Benzimidazol-2-yl)Aniline (BMA) for the detection of phosgene and nitrite in different solvent environments. BMA had fast response (1 min), high selectivity and sensitivity (the limit of detection was 1.27 nM) to phosgene in CH3CN solution (containing 10% DMSO), which manifested as a ratiometric fluorescent mode from 416 nm to 480 nm. The response of BMA to nitrite in HCl solution (pH = 1, containing 10% CH3CN) was also highly selective and sensitive (the limit of detection was 60.63 nM), which shown as a turn-off fluorescent mode at 485 nm. In addition, two portable chemosensors (BMA-loaded TLC plates and test strips) had also been successfully manufactured for the detection of phosgene in the gas phase and nitrite in solution, which displayed good responses. Most importantly, BMA had also been successfully used for detection of nitrite in food samples, and a good recovery (88.5%-107.2%) was obtained by adding standard sodium nitrite.

Sensitive and selective detection of phosgene with a bis-(1H-benzimidazol-2-yl)-based turn-on fluorescent probe in the solution and gas phase.

As one of the chemical weapons in World War I, phosgene (COCl2) is an extremely toxic gas. Due to wide applications in industrial production, phosgene can easily leak inadvertently and poses a serious threat to the environment and human health and safety. In this study, we report for the first time a new fluorescent probe (bis-(1H-benzimidazol-2-yl)-methanone) for the rapid detection of phosgene. The probe is based on the easily prepared bis-(1H-benzimidazol-2-yl), which can quickly combine with phosgene to obtain a six-membered cyclic carbamylation product showing fluorescence turn-on. The combination of phosgene caused the maximum absorption of the probe shifting from 361 nm to 373 nm, which showed a color change from colorless to yellow under visible light. Meanwhile, a strong fluorescence emission peak appeared at 428 nm, which showed change from no fluorescence to blue-violet fluorescence under a UV lamp (365 nm). The response of the probe towards phosgene is fast (within 30 s), highly selective and sensitive (the detection limit in solution being 3.3 nM). Furthermore, the portable test strips manufactured using the probe can conveniently perform visual and fluorescence detection of phosgene in the gas phase.

A FRET-based ratiometric fluorescent probe for sulfide detection in actual samples and imaging in Daphnia magna.

Sulfide plays an important role in many important life processes, and abnormal levels of sulfide that may cause diseases. Sulfide is also essential in industrial production and food processing, and it is well concerned for environmental issues and food safety. In order to study the physiological and pathological effects of sulfide and the impact on the environment, it is still urgent to develop a convenient and effective sulfide detection technology. Here, we developed a ratiometric fluorescent probe 7-Nitro-1,2,3-benzoxoxadiazole-Acridoneacetylpiperazine (NBD-AAP) which is based on the fluorescence resonance energy transfer (FRET) between acridone and NBD fluorophores. The NBD-AAP probe could produce a ratiometric response to micromolar sulfide in buffer (pH = 7.4), exhibiting a significant enhancement in fluorescent emission ratio (F427/F552) and obvious visual phenomenon (orange to pink under visible light and yellow to blue under UV light). At the same time, this NBD-AAP probe also has excellent properties including high selectivity and low detection limit (0.19 µM). In addition, this probe has been successfully used for detecting the sulfide in actual samples (monosodium glutamate, beer, environmental water) and imaging in Daphnia magna. These results indicate that NBD-AAP has broad application prospects in sulfide detection and in vivo imaging studies.

Determination of multiple phytohormones in fruits by high-performance liquid chromatography with fluorescence detection using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling.

Plant hormone determination in food matrices has attracted more and more attention because of their potential risks to human health. However, analytical methods for the analysis of multiple plant hormones remain poorly investigated. In the present study, a convenient, selective, and ultrasensitive high-performance liquid chromatography method for the simultaneous determination of multiple classes of plant hormones has been developed successfully using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling. Eight plant hormones in fruits including jasmonic acid, 12-oxo-phytodienoic acid, indole-3-acetic acid, 3-indolybutyric acid, 3-indolepropionic acid, gibberellin A3 , 1-naphthylacetic acid, and 2-naphthaleneacetic acid were analyzed by this method. The conditions employed for dispersive liquid-liquid microextraction were optimized systematically. The linearity for all plant hormones was found to be >0.9993 (R(2) values). This method offered low detection limits of 0.19-0.44 ng/mL (at a signal-to-noise ratio of 3), and method accuracies were in the range of 92.32-103.10%. The proposed method was applied to determine plant hormones in five kinds of food samples, and this method can achieve a short analysis time, low threshold levels of detection, and a high specificity for the analysis of targeted plant hormones present at trace level concentrations in complex matrices.