RESUMEN
The monoclonal antibodies to Puumala, Dobrava, Hantaan, and Seoul hantaviruses were obtained using mice. The viruses were known to cause HFRS, and two variants of ELISA were designed. First, Hanta-PUU variant, was constructed using monoclonal antibodies to Puumala virus envelope glycoprotein (G(N):G(C)) for detecting only Puumala virus antigen. The second, Hanta-N variant, was constructed using monoclonal antibodies to Dobrava and Puumala nucleocapsid proteins for detecting four above mentioned hantaviruses. Both Hanta-PUU and Hanta-N assays were reliable in detecting specific hantavirus antigens and the immunogenecity of hantavirus vaccines.
Asunto(s)
Anticuerpos Monoclonales de Origen Murino/química , Antígenos Virales/inmunología , Fiebre Hemorrágica con Síndrome Renal/inmunología , Orthohantavirus/inmunología , Proteínas del Envoltorio Viral/inmunología , Vacunas Virales/inmunología , Animales , Anticuerpos Monoclonales de Origen Murino/inmunología , Chlorocebus aethiops , Ensayo de Inmunoadsorción Enzimática/métodos , Humanos , Ratones , Ratones Endogámicos BALB C , Células VeroRESUMEN
The findings suggest that there are natural foci of hantavirus infection in the Tambov Region. There is evidence that Dobrava/Belgrade hantavirus (DOB-Aa) was a leading etiological agent in the outbreak of the disease in the winter of 2006-2007. Epidemiological analysis showed that the outbreak of hemorrhagic fever with renal syndrome (HFRS) afflicted the region during November to April, by reaching its peak in January (52.2%). Among the patients with HFRS, rural dwellers were 91%. People were infected with the virus mainly by taking care of domestic animals (97.2%). The reservoir of the virus and the source of its human infection in the outbreak was a field mouse, its western subspecies Apodemus agrarius agrarius, which was absolutely dominated among all the virus carriers.
Asunto(s)
Anticuerpos Antivirales/sangre , Arvicolinae/virología , Brotes de Enfermedades , Fiebre Hemorrágica con Síndrome Renal , Orthohantavirus/aislamiento & purificación , Adolescente , Adulto , Animales , Niño , Preescolar , Reservorios de Enfermedades , Femenino , Orthohantavirus/clasificación , Orthohantavirus/inmunología , Fiebre Hemorrágica con Síndrome Renal/diagnóstico , Fiebre Hemorrágica con Síndrome Renal/epidemiología , Fiebre Hemorrágica con Síndrome Renal/virología , Humanos , Masculino , Ratones , Persona de Mediana Edad , Federación de Rusia/epidemiología , Zoonosis/transmisión , Zoonosis/virologíaRESUMEN
Adult white rats were exposed daily during 30 days to 2-hour heating (40--41 degrees)--adaptation, and then were transferred to ordinary temperature conditions at 25--27 degrees--desadaptation. The initial period of adaptation revealed a decrease of the liver glycogen content and a relatively constant level of glycemia. A considerable activation of the carbohydrate digestion enzyme systems followed this lowering of energy resources. Metabolic regulatory mechanisms seem to take part in this activation which plays an adaptive role in maintenance of constant glycemia level under these stressful conditions.
Asunto(s)
Adaptación Fisiológica , Glicósido Hidrolasas/metabolismo , Calor , Mucosa Intestinal/enzimología , Glucógeno Hepático/análisis , Amilasas/metabolismo , Animales , Glucemia/análisis , Ratas , Sacarasa/metabolismo , alfa-Amilasas/metabolismo , alfa-Glucosidasas/metabolismoAsunto(s)
Calor , Mucosa Intestinal/enzimología , Adaptación Fisiológica , Animales , Femenino , Masculino , RatasRESUMEN
In acute experiments, the amylolytic, invertase, glycyl-L-tyrosindipeptidase, monoglyceridlipase,and alkaline phosphatase activity of the mucosa homogenate of the rat small intestine was studied 4, 24, 48 and 72 hrs after a two-hour exposure to 36-37 and 40-41 degrees C. No decrease in the activity of any of the ferments was observed. Exposure to heat is concluded to lead to the changes of small intestine ferments spectrum. The character of these changes depends on the heat stress degree and its duration.
Asunto(s)
Calor , Mucosa Intestinal/enzimología , Intestino Delgado/enzimología , Fosfatasa Alcalina/metabolismo , Amilasas/metabolismo , Animales , Dipeptidasas/metabolismo , Femenino , Masculino , Monoacilglicerol Lipasas/metabolismo , Ratas , Sacarasa/metabolismo , Factores de TiempoRESUMEN
It was revealed in acute experiments that exposure to high temperature changed the invertase, dipeptidase, lipolytic and alkaline phosphatase activity of the small intestinal mucosa in rats. The direction of these changes and their degree differed in the thyroidectomized and sham-operated animals. It is suggested that the thyroid hormones took part in the reaction of the fermentative systems of the small intestine to the action of the thermal factor.