RESUMEN
The initial phase of peroxisomal fission requires the peroxisomal membrane protein Peroxin 11 (Pex11p), which remodels the membrane, resulting in organelle elongation. Here, we identify an additional function for Pex11p, demonstrating that Pex11p also plays a crucial role in the final step of peroxisomal fission: dynamin-like protein (DLP)-mediated membrane scission. First, we demonstrate that yeast Pex11p is necessary for the function of the GTPase Dynamin-related 1 (Dnm1p) in vivo. In addition, our data indicate that Pex11p physically interacts with Dnm1p and that inhibiting this interaction compromises peroxisomal fission. Finally, we demonstrate that Pex11p functions as a GTPase activating protein (GAP) for Dnm1p in vitro. Similar observations were made for mammalian Pex11ß and the corresponding DLP Drp1, indicating that DLP activation by Pex11p is conserved. Our work identifies a previously unknown requirement for a GAP in DLP function.
Asunto(s)
GTP Fosfohidrolasas/metabolismo , Membranas Intracelulares/fisiología , Proteínas de la Membrana/metabolismo , Proteínas Mitocondriales/metabolismo , Peroxisomas/fisiología , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/fisiología , Animales , Células COS , Chlorocebus aethiops , Microscopía Electrónica , Microscopía Fluorescente , Modelos Biológicos , Peroxinas , Pichia , Saccharomyces cerevisiae/metabolismoRESUMEN
We demonstrate that the peroxin Pex3 is not required for the formation of peroxisomal membrane structures in yeast pex3 mutant cells. Notably, pex3 mutant cells already contain reticular and vesicular structures that harbor key proteins of the peroxisomal receptor docking complex-Pex13 and Pex14-as well as the matrix proteins Pex8 and alcohol oxidase. Other peroxisomal membrane proteins in these cells are unstable and transiently localized to the cytosol (Pex10, Pmp47) or endoplasmic reticulum (Pex11). These reticular and vesicular structures are more abundant in cells of a pex3 atg1 double deletion strain, as the absence of Pex3 may render them susceptible to autophagic degradation, which is blocked in this double mutant. Contrary to earlier suggestions, peroxisomes are not formed de novo from the endoplasmic reticulum when the PEX3 gene is reintroduced in pex3 cells. Instead, we find that reintroduced Pex3 sorts to the preperoxisomal structures in pex3 cells, after which these structures mature into normal peroxisomes.
Asunto(s)
Proteínas Fúngicas/fisiología , Proteínas de la Membrana/fisiología , Peroxisomas/metabolismo , Pichia/ultraestructura , Autofagia , Proteínas Fúngicas/análisis , Proteínas Fúngicas/genética , Eliminación de Gen , Proteínas Fluorescentes Verdes/análisis , Membranas Intracelulares/metabolismo , Membranas Intracelulares/ultraestructura , Proteínas de la Membrana/análisis , Proteínas de la Membrana/genética , Proteínas de Transporte de Membrana/análisis , Proteínas de Transporte de Membrana/genética , Proteínas de Transporte de Membrana/metabolismo , Microscopía Electrónica de Transmisión , Peroxisomas/ultraestructura , Pichia/genética , Pichia/metabolismo , Ubiquitina-Proteína Ligasas/genéticaRESUMEN
We show that the synthesis of the N-terminal 50 amino acids of Pex3p (Pex3p(1-50)) in Hansenula polymorpha pex3 cells is associated with the formation of vesicular membrane structures. Biochemical and ultrastructural findings suggest that the nuclear membrane is the donor membrane compartment of these vesicles. These structures also contain Pex14p and can develop into functional peroxisomes after subsequent reintroduction of the full-length Pex3p protein. We discuss the significance of this finding in relation to peroxisome reintroduction, e.g. in case peroxisomes are lost due to failure in inheritance.
Asunto(s)
Transportadoras de Casetes de Unión a ATP , Proteínas Fúngicas/fisiología , Proteínas de la Membrana/fisiología , Peroxisomas/metabolismo , Pichia/metabolismo , Proteínas de Saccharomyces cerevisiae , Secuencia de Bases , Cartilla de ADN , Microscopía Fluorescente , Membrana Nuclear/metabolismo , Peroxinas , Pichia/ultraestructuraRESUMEN
Alcohol oxidase (AO) is a peroxisomal enzyme that catalyses the first step in methanol metabolism in yeast. Monomeric, inactive AO protein is synthesised in the cytosol and subsequently imported into peroxisomes, where the enzymatically active, homo-octameric form is found. The mechanisms involved in AO octamer assembly are largely unclear. Here we describe the isolation of Hansenula polymorpha mutants specifically affected in AO assembly. These mutants are unable to grow on methanol and display reduced AO activities. Based on their phenotypes, three major classes of mutants were isolated. Three additional mutants were isolated that each displayed a unique phenotype. Complementation analysis revealed that the isolated AO assembly mutants belonged to 10 complementation groups.