Influence of different fixation methods on the fracture force of osteoporotic human lumbar vertebral bodies in the generation of vertebral compression fractures.

BACKGROUND: The use of fresh-frozen (FF) specimens represents the gold standard for biomechanical investigations. Since FF specimens are often difficult to obtain, chemical-fixed specimens (formalin (FA), Thiel (TH)) are also used. OBJECTIVE: Since fixation methods can alter the mechanical properties of bone tissue, the purpose of this study was to examine their influence on the fracture force of lumbar vertebral bodies (VB). METHODS: First the VB were subdivided into three focus groups: FF, TH, and FA. After removing the soft tissue and the processus transverses of all VB, the endplates were planned with a thin layer of epoxy resin, in order to apply a constant strain to the surface and sub-surface. The VB were subjected to axial compression tests in order to determine fracture force. Lastly a standardized compression fracture was generated. RESULTS: The mean values of the fracture force of the focus groups were 4529.5 N (FF), 3211.3N (TH) and 2947.9N (FA). Consequently a significant difference between the FF and the other two groups could be demonstrated (p< 0.05). CONCLUSION: The preliminary tests showed that the fraction force of fresh-frozen VB were significantly higher than TH/FA-fixed VB. Therefore, these fixation methods could potentially have an influence on the biomechanical properties of VB. This leads to the assumption that if load-to-failure tests are performed, it is probably recommended to use fresh-frozen specimens.

Fracture generation in human vertebrae under compression loading: The influence of pedicle preservation and bone mineral density on in vitro fracture behavior.

BACKGROUND: Fractured vertebral bodies are a common and wide spread health issue. OBJECTIVE: The purpose of this study was to develop a standardized method to experimentally generate compression fractures in vertebral bodies. The influence of the pedicles has been investigated with regards to the fracture behavior. The correlation between bone mineral density (BMD), the cause of fractures and the fracture behavior was investigated. METHODS: Twenty-one fresh frozen human lumbar spines were examined for bone mineral density (BMD) by means of quantitative computed tomography (qCT). All soft tissue was removed, vertebrae were carefully separated from each other and the exposed cranial and caudal endplates were covered with a thin layer of resin to generate a plane and homogeneous surface. A total of 80 vertebral bodies were tested until fracture. RESULTS: A good positive correlation was found between BMD, fracture compression force and stiffness of the vertebral body. No significant differences were found between the fractures generated in vertebral bodies with and without pedicles, respectively. CONCLUSIONS: Our model represents a consolidation of already existing testing devices. The comparative measurement of the BMD and the fracture behavior shows validity. In contrast to other authors, the force was applied to the whole vertebral body. Furthermore the upper and lower plates were not parallelized and therefore the natural anatomic shape was imitated. Fracture behavior was not altered by removing the pedicles.

Modification of PMMA vertebroplasty cement for reduced stiffness by addition of normal saline: a material properties evaluation.

PURPOSE: Vertebral augmentation is an established treatment for patients with pathological vertebral compression fractures. These procedures typically employ a PMMA-based bone cement, which possesses a high compressive stiffness. Because of the increased risk of subsequent fractures after vertebral augmentations, there is a desire for reducing this stiffness. The goal of our study was to examine the influence of adding isotonic saline on the biomechanical properties of PMMA vertebroplasty cement. METHODS: A PMMA-based vertebroplasty cement was prepared according to the manufacturer's recommendations after which isotonic saline was mixed into the cement at 10, 20, and 30% (volume:volume). Testing bodies were cast, and compression and bending tests were performed. Fracture surfaces were studied using SEM. Measurements of injectability, setting temperature, and radioopacity were also performed. RESULTS: The addition of saline solution (of up to vol-30%) led to a pronounced reduction in the compression modulus of the cement from 3409 ± 312 to 1131 ± 127 MPa. In parallel, maximal compression strength was reduced from 86 ± 4 to 33 ± 3 MPa and bending strength from 40 ± 4 to 24 ± 3 MPa. The differences regarding injectability, setting temperature, and radioopacity were small and probably of no clinical relevance. CONCLUSIONS: The compressive stiffness of PMMA-based vertebroplasty cement can be reduced to almost a third by the addition of saline. The probable explanation is an increase in microporosity. Future simulator experiments will show whether the achieved reduction in stiffness is large enough to reduce the rate of subsequent vertebral fractures.

PMMA third-body wear after unicondylar knee arthroplasty decuples the UHMWPE wear particle generation in vitro.

INTRODUCTION: Overlooked polymethylmethacrylate after unicondylar knee arthroplasty can be a potential problem, since this might influence the generated wear particle size and morphology. The aim of this study was the analysis of polyethylene wear in a knee wear simulator for changes in size, morphology, and particle number after the addition of third-bodies. MATERIAL AND METHODS: Fixed bearing unicondylar knee prostheses (UKA) were tested in a knee simulator for 5.0 million cycles. Following bone particles were added for 1.5 million cycles, followed by 1.5 million cycles with PMMA particles. A particle analysis by scanning electron microscopy of the lubricant after the cycles was performed. Size and morphology of the generated wear were characterized. Further, the number of particles per 1 million cycles was calculated for each group. RESULTS: The particles of all groups were similar in size and shape. The number of particles in the PMMA group showed 10-fold higher values than in the bone and control group (PMMA: 10.251 × 10(12); bone: 1.145 × 10(12); control: 1.804 × 10(12)). CONCLUSION: The addition of bone or PMMA particles in terms of a third-body wear results in no change of particle size and morphology. PMMA third-bodies generated tenfold elevated particle numbers. This could favor an early aseptic loosening.

Increase of tibial slope reduces backside wear in medial mobile bearing unicompartmental knee arthroplasty.

BACKGROUND: Unicondylar knee arthroplasty is a good alternative for patients in monocompartmental osteoarthritis. The revision rate in unicondylar knee arthroplasty is higher than in total knee arthroplasty. The influence of the tibial slope on wear in unicondylar knee arthroplasty has not been investigated so far. METHODS: The influence of the tibial slope on wear was investigated in mobile bearing unicondylar knee prosthesis. This was positioned with four different tibial slopes (-4°, 0°, 4°, 8°) in a knee wear simulator simulating the human gait in a plane according to ISO 14243-2:2002(E). After this a kinematic analysis was performed and the inlays were observed under reflected-light-microscopy. FINDINGS: Wear was significantly reduced with an increasing tibial slope (0°: 3.46 mg/millioncycles, SD: 0.59, 4° slope: 1.52 mg/millioncycles, SD: 0.06, 8° slope group: 0.99 mg/millioncycles, SD: 0.42). An anterior slope of -4° also reduced wear (2.08 mg/millioncycles, SD: 0.37). Kinematic analysis revealed a reduced translation between the inlay and the tibia with an increasing tibial slope. The backside of the inlays of the 4° and 8° slope group showed less wear pattern when observed under reflected-light microscopy. INTERPRETATION: Increasing the tibial slope led to a reduced translation between the inlay and the prosthesis in the analysed mobile-bearing unicondylar knee arthroplasty and with this to a reduced backside wear. A tibial slope between 4 and 8° can be recommended in mobile UKA to reduce wear, however, the influence on the ligaments has to be considered and needs to be investigated in further studies.