1.
Biotechniques
; 66(2): 93-95, 2019 02.
Artículo
en Inglés
| MEDLINE
| ID: mdl-30744406
RESUMEN
The potential of genomic DNA is realized when new modalities are invented that manipulate large DNAs with minimal breakage or loss of sample. Here, we describe a polydimethylsiloxane-polycarbonate membrane device to remove small molecules from a sample while retaining large DNAs. Dialysis rates dramatically change as DNA size in kb (M) increases and DNA dimensions become comparable to pore size, and chain characteristics go from rod-like to Gaussian. Consequently, we describe empirical rates of dialysis, R, as a function of M as falling into two regimes: DNAs ≤ 1 kb show R(M) â¼e -t/τM (t = time, τM = time constant), while DNAs ≥1.65 kb slowly passage with R(M) â¼M -1.68; such partitioning potentiates single-molecule imaging.