Catalytic advancements in carbonaceous materials for bio-energy generation in microbial fuel cells: a review.

Microbial fuel cells (MFCs) are a sustainable alternative for wastewater treatment and clean energy generation. The efficiency of the technology is dependent on the cathodic oxygen reduction reaction, where the sluggish reaction kinetics hampers its propensity. Carbonaceous materials with high electrical conductivity have been widely explored for oxygen reduction reaction (ORR) catalysts. Here, incorporating transition metal (TM) and heteroatom into carbon could further enhance the ORR activity and power generation in MFCs. Nitrogen (N)-doped carbons have also been a popular research hotspot due to abundant active sites formed, resulting in superior conductivity, stability, and catalytic activity over carbons. This review summarizes the progress in the carbon-based materials (primary focus on the cathode) for ORR and their utilization in MFCs. Furthermore, we discussed the conceptualization of MFCs and carbonaceous materials to instigate the ORR kinetics and power generation in MFC. Furthermore, prospects of carbon-based materials for actual application in bio-energy generation have been discussed. Carbonaceous catalysts and biomass-derived carbons exhibit good potential to replace precious Pt catalysts for ORR. M-N-C catalysts were found to be the most suitable catalysts. Electrocatalysts with MNx sites are able to achieve excellent activity and high-power output by taking advantage of the active site exposure and rapid mass transfer rate. Moreover, the use of biomass-derived carbons/self-doped carbons could further reduce the overall cost of catalysts. It is anticipated that the research gaps and future perspectives discussed will show new avenues to develop excellent electrocatalysts for better performance and transformation of technology to industrial applications.

Magnesium Cobaltite Embedded in Corncob-Derived Nitrogen-Doped Carbon as a Cathode Catalyst for Power Generation in Microbial Fuel Cells.

Microbial fuel cells (MFCs) have drawn attention among renewable energy devices. High capital cost, poor durability, and slow oxygen reduction reaction (ORR) kinetics are the major hindrances in the commercialization of the technology. In this work, an environmentally benign approach is followed to synthesize a composite of magnesium cobaltite (MgCo2O4) embedded in nitrogen-doped carbon to optimize the performance of MFCs. Detailed characterization confirms the formation of MgCo2O4 nanostructures in the catalyst treated at 700 °C. Electrochemical studies suggest the superior performance of the MgCo2O4/NC-700 catalyst with a peak reduction current of -0.068 mA and a charge transfer resistance (Rct) of 40.5 Ω. The performance of the air cathodes is evaluated using activated sludge as inoculum in the anode chamber in single-chamber MFCs. The power output of MFC with MgCo2O4/NC-700 as an air cathode reaching 873.81 mW m-2 is 59.56 and 216.05% higher than those of MFCs with Pt/C (547.65 mW m-2) and Co/NC-700 (276.48 mW m-2) cathodes, respectively. These findings suggest that substituting magnesium in transition metal-nitrogen-carbon composites could help realize long-term application as air cathodes for power generation and wastewater treatment in MFCs.

Co-Doped Zeolite-GO Nanocomposite as a High-Performance ORR Catalyst for Sustainable Bioelectricity Generation in Air-Cathode Single-Chambered Microbial Fuel Cells.

High-performance cobalt (Co) nanoparticles supported on a zeolite-graphene oxide (1:2) matrix (catalyst Z2) are synthesized through a facile reduction method. In multipoint Brunauer-Emmett-Teller (MBET) surface area analysis, catalyst Z2 demonstrates a higher surface area compared with other synthesized catalysts, indicating the presence of a larger number of catalytic active sites, and supports outstanding ORR performance due to an improved electron-transfer rate and a higher number of redox-active sites. Furthermore, it is observed that catalyst Z2 is an excellent electrocatalytic material due to its low charge-transfer resistance and higher oxygen reduction reaction (ORR) activity. Herein, the electrocatalytic investigation suggests that catalyst Z2 at a potential of 483 mV and a reduction current of -0.382 mA displays a higher electrocatalytic performance and higher stability toward ORR compared with other synthesized catalysts and even the standard Pt/C catalyst. Also, when catalyst Z2 is applied as an air-cathode ORR electrocatalyst for a single-chambered microbial fuel cell (SC-MFC), the SC-MFC coated with catalyst Z2 generates the maximum power density of 416.78 mW/m2, which is 306% higher than that of SC-MFC coated with Pt/C (102.67 mW/m2). In fact, the longer stability and electronic conductivity have contributed to an outstanding ORR activity of the nanocomposite due to its porous surface morphology and the presence of the functional groups in the zeolite-GO support matrix. In brief, Co (cobalt) nanoparticles doped on a zeolite-GO (1:2) support matrix are promising cathode electrocatalysts in the practical application of MFCs and other related devices.

Copper nanoparticles embedded in polyaniline derived nitrogen-doped carbon as electrocatalyst for bio-energy generation in microbial fuel cells.

Microbial fuel cells (SC-MFCs) have emerged as green energy devices to resolve the growing energy and environmental crisis. However, the technology's application depends on the sluggish oxygen reduction reaction (ORR) kinetics. Among the electrocatalysts explored, transition metal-nitrogen-carbon composites exhibit satisfactory ORR activity. Herein, we investigate the performance of copper-nitrogen-carbon (Cu/NC) electrocatalysts for ORR, highlighting the effect of temperature, role of nitrogen functionalities, and Cu-Nx sites in catalyst performance. Cu/NC-700 demonstrated satisfactory ORR activity with an onset potential of 0.7 V (vs. RHE) and a limiting current density of 3.4 mA cm-2. Cu/NC-700 modified MFC exhibited a maximum power density of 489.2 mW m-2, higher than NC-700 (107.3 mW m-2). These observations could result from synergistic interaction between copper and nitrogen atoms, high density of Cu-Nx sites, and high pyridinic-N content. Moreover, the catalyst exhibited superior stability, implying its use in long-term operations. The electrocatalytic performance of the catalyst suggests that copper-doped carbon catalysts could be potential metal-nitrogen-carbon material for scaled-up MFC applications.

AgNPs Ornamented Modified Bacterial Cellulose Based Self-Healable L-B-L Assembly via a Schiff Base Reaction: A Potential Wound Healing Patch.

A layer-by-layer (L-B-L) bacterial cellulose (BC)-based transdermal patch has been prepared via a Schiff base reaction. The L-B-L assembly consisting of covalently cross-linked ethylene diamine-modified carboxymethylated BC isolated from the Glucanoacetobacter xylinus (MTCC7795) bacterial strain and aldehyde-modified pectin formed via a Schiff base reaction. The presence of the imine bond assists the self-healing process after being scratched in the presence of a pH 7.4 buffer solution monitored via optical microscopy, atomic force microscopy, and tensile strength analyses. The formation of the L-B-L assembly was confirmed using field-emission scanning electron microscopy (FESEM) analysis. Simultaneously, water swelling and deswelling studies were carried out to test its water retention efficiency. The presence of silver nanoparticles (AgNPs) has been confirmed by ultraviolet-visible spectroscopy and FESEM analyses. The antimicrobial activity of the AgNPs-incorporated transdermal patch has been examined over Staphylococcus aureus and Escherichia coli using the zone of inhibition method. Additionally, the cell viability assay was performed using the fluorescent dyes 4',6-diamidino-2-phenylindole and propidium iodide. The AgNPs in the L-B-L assembly showed antimicrobial property against both types of bacteria. The cytotoxicity and wound healing property of the patch system have been studied over NIH 3T3 fibroblast and A549 epithelial cell lines. The L-B-L film also influenced the wound healing process of these two cell lines.

Performance evaluation of poly(aniline-co-pyrrole) wrapped titanium dioxide nanocomposite as an air-cathode catalyst material for microbial fuel cell.

A simple, inexpensive in situ oxidative polymerization of aniline and pyrrole using ammonium persulfate (APS) as oxidant and hydrochloric acid (HCl) as dopant has been used to synthesize a hybrid (PAni-Co-PPy)@TiO2 nanocomposite with titanium oxide (TiO2) nanoparticles (NPs) wrapped into (PAni-Co-PPy) copolymer. The synthesized nanocomposite has been shown with higher oxygen reduction reactions (ORR) as an excellent cathode material for higher performance in the complex of (PAni-Co-PPy)+/TiO2(O-). The charge transport phenomenon between TiO2 and (PAni-Co-PPy)+ were found adequate with subsequent delocalization of electron/s at PAni and PPy. The self-doping nature of TiO2 (O-) played a vital role in oxygen adsorption and desorption process. With higher electrical conductivity and surface area, these were tested in microbial fuel cells (MFCs) for ORRs at cathode. This yielded a relatively higher current and power density output as compared to PAni@TiO2, PPy@TiO2, and commercially available Pt/C cathode catalysts in MFC system. In overall, the prepared (PAni-Co-PPy)@TiO2 nano-hybrid cathode delivered ~2.03 fold higher power density as compared to Pt/C catalyst, i.e. ~987.36 ± 49 mW/m2 against ~481.02 ± 24 mW/m2. The properties of electro-catalysts established an improved synergetic effect between TiO2 NPs and (PAni-Co-PPy). In effect, the enhanced surface area and electrochemical properties of the prepared (PAni-Co-PPy)@TiO2 nano-hybrid system is depicted here as an effective cathode catalyst in MFCs for improved performance.

Hydrothermal synthesis of gelatin quantum dots for high-performance biological imaging applications.

In the present study, we are reporting a one-pot synthesis of gelatin quantum dots (GeQDs) by the hydrothermal process. The synthesized GeQDs were characterized by fourier transform infrared spectroscopy, nuclear magnetic resonance, ultraviolet-visible and photoluminescence spectroscopic techniques, and also by using high-resolution transmission electron microscopy. The GeQDs showed a high level of photoluminescence quantum yield (PLQY) with significantly higher stability for up to 6 months and presented similar fluorescent intensity as the initial PLQY without any precipitation and aggregation at ambient condition. The cell imaging ability of synthesized GeQDs was examined using cells belonging to diverse clinical backgrounds like bacterial cells including Escherichia coli and Staphylococcus aureus, yeast cells including Candida albicans, C. krusei, C. parapsilosis, and C. tropicalis, mycelial fungi including Aspergillus flavus and A. fumigatus cells, cancer cell lines A549, HEK293 and L929. The results demonstrated that the GeQDs illuminates the cells and can be utilized as potential cell labeling non-toxic biomarkers. In conclusion, it can be said that the gelatin stabilized QDs are a promising candidate for stable and long-term fluorescent imaging of different types of cells.

A review on the synthesis of graft copolymers of chitosan and their potential applications.

Chitosan is an antimicrobial, biodegradable and biocompatible natural polymer, commercially derived from the partial deacetylation of chitin. Currently modified chitosan has occupied a major part of scientific research. Modified chitosan has excellent biotic characteristics like biodegradation, antibacterial, immunological, metal-binding and metal adsorption capacity and wound-healing ability. Chitosan is an excellent candidate for drug delivery, food packaging and wastewater treatment and is also used as a supporting object for cell culture, gene delivery and tissue engineering. Modification of pure chitosan via grafting improves the native properties of chitosan. Chitosan grafted copolymers exhibit high significance and are extensively used in numerous fields. In this review, modifications of chitosan through several graft copolymerization techniques such as free radical, radiation, and enzymatic were reported and the properties of grafted chitosan were discussed. This review also discussed the applications of grafted chitosan in the fields of drug delivery, food packaging, antimicrobial, and metal adsorption as well as dye removal.

Sulfonated graphene oxide and titanium dioxide coated with nanostructured polyaniline nanocomposites as an efficient cathode catalyst in microbial fuel cells.

In this study, sulfonated graphene oxide (SGO) was synthesized as potential conducting matrix to improve the properties of catalyst for single chamber microbial fuel cells (SC-MFCs). Here, TiO2 and Polyaniline (PAni) nanoparticles were anchored over SGO and the resulting SGO-TiO2-PAni nanocomposites were used as a potential cathode catalyst in MFCs. We have also examined the performance of SGO-TiO2-PAni compared to GO-TiO2-PAni and TiO2-PAni catalyst. The structural and morphological analyses were examined using a variety of characterization techniques. TiO2 nanoparticles bridged PAni and SGO through hydrogen bonding/electrostatic interaction and improved the thermal stability of SGO-TiO2-PAni catalyst. The electrochemical characterizations of these nanocatalysts suggest that the SGO-TiO2-PAni showed higher reduction current value (-0.46 mA), enhanced stability, and lower internal resistance (46.2 Ω) in comparison to GO-TiO2-PAni and TiO2-PAni towards oxygen reduction reactions (ORR). Consequently, MFC using SGO-TiO2-PAni demonstrated a maximum power density of 904.18 mWm-2 than that of GO-TiO2-PAni (734.12 mWm-2), TiO2-PAni (561.5 mWm-2) and Pt/C (483.5 mWm-2). The enhanced catalytic activity of SGO-TiO2-PAni catalyst was ascribed to the high electronic conductivity and long-term permanence of the nanocomposite. These superior electrochemical results suggested that the SGO-TiO2-PAni catalyst could be applied as a potential alternative to the commercial Pt/C cathode catalyst for the application of MFCs.

TiO2 doped chitosan/poly (vinyl alcohol) nanocomposite film with enhanced mechanical properties for application in bone tissue regeneration.

Here, TiO2 nanoparticles have been doped into the polymer film-construct of Chitosan/poly (vinyl alcohol)/Nano-hydroxyapatite (CPHT I - III) to enhance the mechanical and biological properties of the film so as to mimic the human bone extracellular matrix for application in human bone regeneration. The synthesized films are highly porous in nature along with the presence of macrovoids. Significantly enhanced mechanical properties were obtained upon the addition of TiO2 in comparison to previous literature. Increasing content of n-HAP-TiO2 increased the elasticity, tensile strength of the films and the antibacterial efficacy against both Gram-Positive and Gram-Negative Bacteria. The pH of CPHT I-III films in saline remained in the low alkalinity range of (7.48-7.53) on day 14. CPHT I-III films were compatible with the human erythrocytes as their hemolysis was well below the limit of acute hemolysis. The in-vitro studies revealed the highly cytocompatible nature of CPHT III (15% n-HAP-TiO2) for osteoblast-like MG - 63 cell attachment and proliferation. The study has revealed that CPHT III has the potential to be used for bone tissue regeneration, our future studies will be focused on the in-vivo investigations to establish its use in clinical settings.

TiO2 doped chitosan/hydroxyapatite/halloysite nanotube membranes with enhanced mechanical properties and osteoblast-like cell response for application in bone tissue engineering.

The current therapeutic strategies for healing bone defects commonly suffer from the occurrence of bacterial contamination on the graft, resulting in nonunion in the segmental bone defects and the requirement for secondary surgery to remove or sterilize the primary graft. A membrane with enhanced anti-bacterial efficacy, mechanical strength and osteoconductivity would represent an improvement in the therapeutic strategy for guided bone regeneration. The present study aims to optimize the content of halloysite nanotubes (HNTs) and TiO2 in the polymer matrix of chitosan (CTS) with a constant amount of nano-hydroxyapatite (5%) with the objective of mimicking the mechanical and biological microenvironment of the natural bone extracellular matrix with enhanced anti-bacterial efficacy. HNTs are a low-cost alternative to MWNCTs for enhancing the mechanical properties and anti-bacterial efficacy of the composite. From the first stage of the study, it was concluded that the membranes possessed enhanced mechanical properties and optimum biological properties at 7.5% (w/w) loading of HNTs in the composite. In the second stage of this investigation, we studied the effect of the addition of TiO2 nanoparticles (NPs) and TiO2 nanotubes (NTs) in small amounts to the CTS/n-HAP/HNT nanocomposite at 7.5% HNT loading, with an aim to augment the anti-bacterial efficacy and osteoconductivity of this mechanically strong membrane. The study revealed a significant enhancement in the anti-bacterial efficacy, osteoblast-like MG-63 cell proliferation and ALP expression with the addition of TiO2 NTs. The CHH-TiT membrane successfully inhibited the S. aureus and E. coli growth within 16 hours and simultaneously assisted the enhanced proliferation of osteoblast-like cells on its surface. The study supports the potential exploitation of CHH-TiT (7.5% HNT & 0.2% TiO2 NT) membranes as a template for guided bone tissue regeneration.

Curcumin entrapped gelatin/ionically modified bacterial cellulose based self-healable hydrogel film: An eco-friendly sustainable synthesis method of wound healing patch.

In this context, we have prepared a biocompatible gelatin based polyelectrolyte hydrogel patch that has an inherent ability to self-heal in the presence of physiological pH (pH = 7.4). The gelatin-based hydrogel patch consists of the ionically modified self-assembled bacterial cellulose (iBC), extracted from Glucanoacetobacter xylinus (MTCC7795) bacterial strain. Presence of the iBC provides a sturdy cage to the gelatin matrix and also participates into the self-healing activity via formation of the ionic interlocking system in the presence of buffer solution having a pH of 7.4 after being damaged. The self-healing activity of the patch has been monitored through tensile strength measurement and AFM depth profilometry analyses. Loading of the curcumin in the hydrogel patch system incorporates the wound healing activity, examined over the NIH 3 T3 fibroblast cell line. The patch is also able to show antimicrobial activity which has been assessed via FESEM analysis and live-dead assay using propidium iodide (PI) and 4',6-diamidino-2-phenylindole (DAPI) as a fluorescent indicator. This self-healable, ionically interlocked, mechanically robust, bio-derived smart hydrogel patch system can pave a new direction in the transdermal drug delivery system.

Polyhydroxybutyrate-co-hydroxyvalerate copolymer modified graphite oxide based 3D scaffold for tissue engineering application.

In this study, we have fabricated the PHBV functionalized graphite oxide using freeze drying technique, followed by 'in situ' pay loading of Fe3O4 nanoparticles onto the hydrophobic plate of the composite basal plane; thereby, mechanically and thermally stable, bio-imaging Fe3O4/GO-g-PHBV composites have been developed. The synthesis of Fe3O4/GO-g-PHBV composite was confirmed by field emission SEM and TEM analyses, X-ray diffraction and Fourier transform infrared spectroscopy. The wrapping of PHBV copolymer into the graphene layers was investigated by atomic force microscopy and Raman spectral analyses which provided the shifting of the 2D band with low signal intensity in the range of 2600-3000 cm-1. The bactericidal activities of the Fe3O4/GO-g-PHBV composite films were found to exhibit more efficiency against Gram-negative bacteria strains compared to Gram-positive strains. In vibrating sample magnetometer (VSM) analysis, the zero value of coercivity revealed the super-paramagnetic nature of the Fe3O4/GO-g-PHBV composites. The Phantom agar magnetic resonance imaging analysis revealed the efficiency of Fe3O4 nanoparticles as a negative contrast (T2 contrast) along with higher relaxivity value. The significant fibroblast cell (NIH 3T3) adhesion and proliferation (85%) on the Fe3O4/GO-g-PHBV composite surface indicated the physiological and biocompatible stability of that composite along with the presence of large π conjugated aromatic domain.

Organically modified clay supported chitosan/hydroxyapatite-zinc oxide nanocomposites with enhanced mechanical and biological properties for the application in bone tissue engineering.

The objective of this study is to design biomimetic organically modified montmorillonite clay (OMMT) supported chitosan/hydroxyapatite-zinc oxide (CTS/HAP-ZnO) nanocomposites (ZnCMH I-III) with improved mechanical and biological properties compared to previously reported CTS/OMMT/HAP composite. Fourier transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy and transmission electron microscopy were used to analyze the composition and surface morphology of the prepared nanocomposites. Strong antibacterial properties against both Gram-positive and Gram-negative bacterial strains were established for ZnCMH I-III. pH and blood compatibility study revealed that ZnCMH I-III should be nontoxic to the human body. Cytocompatibility of these nanocomposites with human osteoblastic MG-63 cells was also established. Experimental findings suggest that addition of 5wt% of OMMT into CTS/HAP-ZnO (ZnCMH I) gives the best mechanical strength and water absorption capacity. Addition of 0.1wt% of ZnO nanoparticles into CTS-OMMT-HAP significantly enhanced the tensile strengths of ZnCMH I-III compared to previously reported CTS-OMMT-HAP composite. In absence of OMMT, control sample (ZnCH) also showed reduced tensile strength, antibacterial effect and cytocompatibility with osteoblastic cell compared to ZnCMH I. Considering all of the above-mentioned studies, it can be proposed that ZnCMH I nanocomposite has a great potential to be applied in bone tissue engineering.

Alginate coated chitosan core-shell nanoparticles for efficient oral delivery of naringenin in diabetic animals-An in vitro and in vivo approach.

The chemical synthesis of this study targets for development of a bio-safe polymeric nano-vehicle for improvising the solubility of the flavanone naringenin in antidiabetic animal study. Nanoparticles were prepared from two cost-effective carbohydrate biopolymers - chitosan and alginate for successful encapsulation of naringenin. Dual crosslinked nanoparticles were synthesized by using Na2SO4 and CaCl2 as crosslinkers. The nanoparticles were characterized by DLS, FTIR, XRD and SEM. The prepared nano-formulations exhibited significant naringenin entrapment of >90% and pH-responsive slow and sustained release of the flavonoid. In-vivo studies revealed significant hypoglycemic effect after oral delivery of the nanoparticles to streptozotocin-induced diabetic rats. Histopathology and several blood parameters indicated that oral administrations of nanoparticles were free from toxicity. Other studies also suggested that polymeric formulations were quite effective for oral delivery of the flavonoid as a therapeutic agent in the treatment of dyslipidemia, hyperglycemia and haemoglobin iron-mediated oxidative stress in type 1 diabetic model.

Development of bone-like zirconium oxide nanoceramic modified chitosan based porous nanocomposites for biomedical application.

Here, zirconium oxide nanoparticles (ZrO2 NPs) were incorporated for the first time in organic-inorganic hybrid composites containing chitosan, poly(ethylene glycol) and nano-hydroxypatite (CS-PEG-HA) to develop bone-like nanocomposites for bone tissue engineering application. These nanocomposites were characterized by FT-IR, XRD, TEM combined with SAED. SEM images and porosity measurements revealed highly porous structure having pore size of less than 1µm to 10µm. Enhanced water absorption capacity and mechanical strengths were obtained compared to previously reported CS-PEG-HA composite after addition of 0.1-0.3wt% of ZrO2 NPs into these nanocomposites. The mechanical strengths and porosities were similar to that of human spongy bone. Strong antimicrobial effects against gram-negative and gram-positive bacterial strains were also observed. Along with getting low alkalinity pH (7.4) values, similar to the pH of human plasma, hemocompatibility and cytocompatibility with osteoblastic MG-63 cells were also established for these nanocomposites. Addition of 15wt% HA-ZrO2 (having 0.3wt% ZrO2 NPs) into CS-PEG (55:30wt%) composite resulted in greatest mechanical strength, porosity, antimicrobial property and cytocompatibility along with suitable water absorption capacity and compatibility with human pH and blood. Thus, this nanocomposite could serve as a potential candidate to be used for bone tissue engineering.

Multifunctional zirconium oxide doped chitosan based hybrid nanocomposites as bone tissue engineering materials.

This paper reports the development of multifunctional zirconium oxide (ZrO2) doped nancomposites having chitosan (CTS), organically modified montmorillonite (OMMT) and nano-hydroxyapatite (HAP). Formation of these nanocomposites was confirmed by various characterization techniques such as Fourier transform infrared spectroscopy and powder X-ray diffraction. Scanning electron microscopy images revealed uniform distribution of OMMT and nano-HAP-ZrO2 into CTS matrix. Powder XRD study and TEM study revealed that OMMT has partially exfoliated into the polymer matrix. Enhanced mechanical properties in comparison to the reported literature were obtained after the addition of ZrO2 nanoparticle into the nanocomposites. In rheological measurements, CMZH I-III exhibited greater storage modulus (G') than loss modulus (G″). TGA results showed that these nanocomposites are thermally more stable compare to pure CTS film. Strong antibacterial zone of inhibition and the lowest minimum inhibition concentration (MIC) value of these nanocomposites against bacterial strains proved that these materials have the ability to prevent bacterial infection in orthopedic implants. Compatibility of these nanocomposites with pH and blood of human body was established. It was observed from the swelling study that the swelling percentage was increased with decreasing the hydrophobic OMMT content. Human osteoblastic MG-63 cell proliferations were observed on the nanocomposites and cytocompatibility of these nanocomposites was also established. Moreover, addition of 5wt% OMMT and 5wt% nano-HAP-ZrO2 into 90wt% CTS matrix provides maximum tensile strength, storage modulus, aqueous swelling and cytocompatibility along with strong antibacterial effect, pH and erythrocyte compatibility.

Development of biomimetic nanocomposites as bone extracellular matrix for human osteoblastic cells.

Here, we have developed biomimetic nanocomposites containing chitosan, poly(vinyl alcohol) and nano-hydroxyapatite-zinc oxide as bone extracellular matrix for human osteoblastic cells and characterized by Fourier transform infrared spectroscopy, powder X-ray diffraction. Scanning electron microscopy images revealed interconnected macroporous structures. Moreover, in this study, the problem related to fabricating a porous composite with good mechanical strength has been resolved by incorporating 5wt% of nano-hydroxyapatite-zinc oxide into chitosan-poly(vinyl alcohol) matrix; the present composite showed high tensile strength (20.25MPa) while maintaining appreciable porosity (65.25%). These values are similar to human cancellous bone. These nanocomposites also showed superior water uptake, antimicrobial and biodegradable properties than the previously reported results. Compatibility with human blood and pH was observed, indicating nontoxicity of these materials to the human body. Moreover, proliferation of osteoblastic MG-63 cells onto the nanocomposites was also observed without having any negative effect.

Interaction between oxidized polyaniline and oppositely charged amphiphilic assemblies in an aqueous/organic biphasic system.

Amphiphilic assemblies (AAs) are known to interact with polyelectrolytes in such a manner that the dynamic AAs retain their structural features, but the polyelectrolytes undergo conformational changes. This article reports that a charge bearing, rigid, water insoluble and oxidized conjugated polymer, polyaniline, can withstand such conformational changes and at the same time force the AAs to disassemble. An interfacial setup, comprising of an aqueous/organic interface, was utilized to study the disassembly process and the subsequent phase transfer phenomenon of the in situ synthesized polymer. It has further been demonstrated that the phase transfer occurred only when the concentration of the amphiphile was at and above its critical aggregation concentration. Moreover, fine dispersions of polyaniline were obtained initially in the aqueous phase and later in the organic phase. These fine dispersions suggest possibilities for better processing of this otherwise "difficult to process" polymer. The disassembly phenomenon was followed by changes in fluorescence emission and UV-vis absorption spectra of an entrapped probe molecule, recorded before and after the interaction. Changes in particle size upon disassembly were studied by dynamic light scattering. Dispersions of the polymer in the two phases were realized from transmission electron micrographs and UV-vis absorption spectra of the dispersed polymer.

Amphiphiles as hydrophobicity regulator: fine tuning the surface hydrophobicity of an electropolymerized film.

Smart clean surfaces exhibiting reversible superhydrophilic to superhydrophobic transitions have been widely fabricated. A myriad of complex fabricating procedures have evolved over the years. Here, we demonstrate an apparently simple approach, wherein we utilized the attractive interaction between an unfabricated electropolymerized film and negative charge bearing amphiphilic molecules to precisely control the hydrophobicity of the surface of the film. We also demonstrate the use of different hydrophobic molecules to plug the voids created by the uneven distribution of the attached amphiphiles' long hydrocarbon tails on the film surface. Water drop contact angle revealed that by the used technique, surface hydrophobicity can be tuned to any desired value. Moreover, the film surface exhibited a high adhesive force toward the water droplets, thus preventing them from rolling or dropping down. In addition, the tuning process can be completely reversed and repeated by altering the applied potential. The interaction between the amphiphiles and the film surface was confirmed by ATR-IR. Scanning electron micrographs revealed that the film surface underwent no morphological modifications upon interaction with the amphiphiles.