RESUMEN
OBJECTIVE: Our study aims to evaluate the various correlations between demographic, biochemical, ultrasound, and ovarian stimulation parameters with the percentage of mature oocytes in conventional stimulation for IVF/ICSI cycles in order to develop a predictive model to improve our understanding of the oocyte maturation process. METHODS: This is a retrospective cohort study; patients underwent conventional antagonist ovarian stimulation protocols for fresh IVF/ICSI cycles. A total of 256 IVF/ICSI cycles were included. Age, antral follicle count (AFC), baseline serum follicle-stimulating hormone (FSH) levels, baseline serum luteinizing hormone (LH) levels, baseline serum estradiol (E2) levels, peak estradiol, P4 on hCG day, the body mass index (BMI), and stimulation length were measured. The variables were tested for correlations with the number of retrieved oocytes (#RO) and the number of mature oocytes (#MO). A backward stepwise regression was performed to identify the variables that correlated more strongly with percentage of mature oocytes (%MO). RESULTS: A predictive equation was obtained with the variables that were not excluded in the model. % MO = 72.700 - 0.910 (Age) + 0.979 (BMI) + 1.209 (Baseline serum LH) - 0.647 (Progesterone on human Chorionic Gonadotropin day). CONCLUSIONS: We concluded that age, the BMI, baseline serum LH, and progesterone level on hCG day may predict %MO. Prospective studies are required to validate this predictive equation.
Asunto(s)
Inducción de la Ovulación , Inyecciones de Esperma Intracitoplasmáticas , Gonadotropina Coriónica , Estradiol/sangre , Femenino , Fertilización In Vitro , Hormona Folículo Estimulante/sangre , Humanos , Hormona Luteinizante/sangre , Oocitos , Estudios RetrospectivosRESUMEN
INTRODUCTION: In this study, we determined normal levels of dysferlin expression in CD14+ monocytes by flow cytometry (FC) as a screening tool for dysferlinopathies. METHODS: Monocytes from 183 healthy individuals and 29 patients were immunolabeled, run on an FACScalibur flow cytometer, and analyzed by FlowJo software. RESULTS: The relative quantity of dysferlin was expressed as mean fluorescence intensity (MFI). Performance of this diagnostic test was assessed by calculating likelihood ratios at different MFI cut-off points, which allowed definition of 4 disease classification groups in a simplified algorithm. CONCLUSION: The MFI value may differentiate patients with dysferlinopathy from healthy individuals; it may be a useful marker for screening purposes. Muscle Nerve 54: 1064-1071, 2016.
Asunto(s)
Proteínas de la Membrana/metabolismo , Monocitos/metabolismo , Proteínas Musculares/metabolismo , Distrofia Muscular de Cinturas/diagnóstico , Distrofia Muscular de Cinturas/patología , Adulto , Algoritmos , Análisis Mutacional de ADN , Disferlina , Femenino , Citometría de Flujo , Humanos , Receptores de Lipopolisacáridos/metabolismo , Masculino , Tamizaje Masivo , Proteínas de la Membrana/genética , Persona de Mediana Edad , Proteínas Musculares/genética , Músculo Esquelético/metabolismo , Distrofia Muscular de Cinturas/genética , Mutación/genética , Estadísticas no Paramétricas , Adulto JovenRESUMEN
OBJECTIVE: To determine the prognosis factors in Mexican patients with Bell's palsy. DESIGN: We designed a prospective, longitudinal, descriptive, and observational analysis. Two hundred and fifty one patients diagnosed with Bell's palsy at the National Institute of Rehabilitation were included. We studied the sociodemographic characteristics, seasonal occurrence, sidedness, symptoms, and therapeutic options to determine the prognostic factors for their recovery. RESULTS: Thirty-nine percent of patients had a complete recovery and 41.5% had an incomplete recovery. Marital status, gender, etiology, symptoms, sidedness, House-Brackmann grade, and treatments did not represent significant prognostic factors for recovery. Age > 40 years (OR = 2.4, IC 95% 1.3-4.3, p = 0.002) and lack of physical therapy (OR = 6.4, IC 95% 1.4-29.6, p = 0.006) were significant prognostic factors for incomplete recovery. Familial palsy resulted to be a protective prognostic factor against an incomplete recovery (OR = 0.54, IC 95% 0.28-1.01, p = 0.039). This protection factor was only significant in female patients (OR = 0.41, p = 0.22) but not in male patients (OR = 1.0, p = 0.61). CONCLUSIONS: The proportion of cases with incomplete recovery was high. The age > 40 years and lack of physical therapy were the only significant prognostic factors for an incomplete recovery.
Asunto(s)
Parálisis de Bell/rehabilitación , Academias e Institutos/estadística & datos numéricos , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Parálisis de Bell/diagnóstico , Parálisis de Bell/epidemiología , Parálisis de Bell/genética , Parálisis de Bell/virología , Niño , Preescolar , Comorbilidad , Terapia por Estimulación Eléctrica/estadística & datos numéricos , Terapia por Ejercicio/estadística & datos numéricos , Femenino , Estudios de Seguimiento , Humanos , Masculino , Conceptos Meteorológicos , México/epidemiología , Persona de Mediana Edad , Modalidades de Fisioterapia/estadística & datos numéricos , Pronóstico , Estudios Prospectivos , Recuperación de la Función , Centros de Rehabilitación/estadística & datos numéricos , Factores de Riesgo , Factores Socioeconómicos , Resultado del Tratamiento , Adulto JovenRESUMEN
Con el propósito de estimar las verdaderas diferencias entre diversos autores respecto a los porcentajes de complicaciones patelares (con o sin prótesis patelar) en la artroplastia total de rodilla (ATR), se realizó un estudio de metanálisis midiendo los tamaños del efecto (ES), las tasas de reducción del riesgo y el número de pacientes a tratar (NNT). En general, se demuestra que los estudios con muestras pequeñas introducen errores aletorios elevados y, estrictamente, no deberían ser considerados para estimar los tamaños del efecto al comparar los grupos tratados (ATR con prótesis patelar) contra los controles (ATR sin prótesis patelar). Se concluye que, al hacer las comparaciones correctas, los porcentajes de complicaciones son significativamente menores en la ATR con prótesis patelar y que las diferencias en los porcentajes de complicaciones, reportadas a partir de estudios con casuística relativamente grande, entre estos autores, son producto de los diferentes grados de dominio de las técnicas quirúrgicas empleadas. Se finaliza recomendando el uso de los estudios de metanálisis en ortopedia