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1.
Metabolites ; 14(7)2024 Jun 30.
Artículo en Inglés | MEDLINE | ID: mdl-39057694

RESUMEN

We conducted this single-center, retrospective, cohort study to examine whether insulin resistance (IR) and high-sensitivity C-reactive protein (hsCRP) have a relationship with metabolic abnormalities in patients with type 2 diabetes mellitus (T2DM). In a total of 3758 patients (n = 3758) with T2DM, we analyzed medical records and thereby evaluated their baseline characteristics such as age, sex, duration of T2DM, systolic blood pressure (SBP), diastolic blood pressure (DBP), waist circumference, body mass index (BMI), visceral fat thickness (VFT), fasting plasma insulin levels, C-peptide levels, glycated hemoglobin (HbA1c), fasting plasma glucose (FPG), postprandial plasma glucose (PPG), homeostatic model assessment of insulin resistance (HOMA-IR), homeostatic model assessment of ß-cell function (HOMA-ß), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), albuminuria, intima-media thickness (IMT) and hsCRP. The patients were stratified according to the tertile of the K index of the insulin tolerance test (KITT) or hsCRP. Thus, they were divided into the lowest (≥2.37), middle (1.54-2.36) and highest tertile (0-1.53) of KITT and the lowest (0.00-0.49), middle (0.50-1.21) and highest tertile (≥1.22) of hsCRP. Moreover, associations of KITT and hsCRP with metabolic abnormalities, such as steatotic liver disease (SLD), metabolic syndrome (MetS), albuminuria, diabetic retinopathy and carotid atherosclerosis, were also analyzed. There was a significant positive correlation between the prevalence of SLD, MetS, albuminuria and diabetic retinopathy and KITT (p < 0.001). Moreover, there was a significant positive association between the prevalence of SLD, MetS and albuminuria and hsCRP (p < 0.001). In conclusion, our results indicate that clinicians should consider the relationships of IR and hsCRP with metabolic abnormalities in the management of patients with T2DM. However, further large-scale, prospective, multi-center studies are warranted to confirm our results.

2.
J Clin Med ; 13(11)2024 May 31.
Artículo en Inglés | MEDLINE | ID: mdl-38892973

RESUMEN

Background: Fexuprazan (Fexuclue®; Daewoong Pharmaceutical Co., Ltd., Seoul, Korea) is a novel potassium-competitive acid blocker (P-CAB). This multi-center, randomized, double-blind, active-controlled, parallel-group, therapeutic confirmatory, phase III study was conducted to assess its efficacy and safety compared with esomeprazole (Nexium®; AstraZeneca, Gothenburg, Mölndal, Sweden) in Korean patients with erosive esophagitis (EE). Methods: This study evaluated patients diagnosed with EE at a total of 25 institutions in Korea between 13 December 2018 and 7 August 2019. After voluntarily submitting a written informed consent form, the patients were evaluated using a screening test and then randomized to either of the two treatment arms. The proportion of the patients who achieved the complete recovery of mucosal breaks at 4 and 8 weeks, the proportion of those who achieved the complete recovery of heartburn at 3 and 7 days and 8 weeks, and changes in the GERD-Health-Related Quality of Life Questionnaire (GERD-HRQL) scores at 4 and 8 weeks from baseline served as efficacy outcome measures. The incidence of treatment-emergent adverse events (TEAEs) and adverse drug reactions (ADRs) and the serum gastrin levels served as safety outcome measures. Results: The study population comprised a total of 231 patients (n = 231) with EE, including 152 men (65.80%) and 79 women (34.20%); their mean age was 54.37 ± 12.66 years old. There were no significant differences in the efficacy and safety outcome measures between the two treatment arms (p > 0.05). Conclusions: It can be concluded that the efficacy and safety of Fexuclue® are not inferior to those of esomeprazole in Korean patients with EE.

3.
J Vet Sci ; 25(3): e24, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38834504

RESUMEN

IMPORTANCE: In veterinary forensic science, accurately determining the postmortem interval (PMI) is crucial for identifying the causes of animal deaths. Autolysis, a significant postmortem process, influences PMI estimation, but its relationship with humidity is not well understood. OBJECTIVE: This study aimed to improve the accuracy of PMI estimates in veterinary forensic cases by looking into how different humidity levels affect autolysis in different organs of rats. METHODS: The study involved 38 male rats, examining histopathological changes in their heart, liver, and pancreas. These organs were subjected to controlled humidity levels (20%, 55%, and 80%) at a constant 22°C. Tissue samples were collected at several intervals (0 h, 12 h, 24 h, 3 days, and 8 days) for comprehensive analysis. RESULTS: Distinct autolytic characteristics in animal organs emerged under varying humidity conditions. The low-humidity environment rapidly activated autolysis more than the high-humidity environment. In addition, it was found that lower humidity caused nuclear pyknosis, cytoplasmic disintegration, and myofiber interruption. The liver, in particular, showed portal triad aggregation and hepatocyte individuation. The pancreas experienced cell fragmentation and an enlarged intracellular space. High humidity also caused the loss of striations in cardiac tissues, and the liver showed vacuolation. Under these conditions, the pancreas changed eosinophilic secretory granules. CONCLUSIONS AND RELEVANCE: The study successfully established a clear connection between the autolytic process in PMIs and relative humidity. These findings are significant for developing a more accurate and predictable method for PMI estimation in the field of veterinary forensic science.


Asunto(s)
Humedad , Hígado , Páncreas , Cambios Post Mortem , Animales , Masculino , Ratas , Hígado/patología , Páncreas/patología , Miocardio/patología , Ratas Sprague-Dawley , Autólisis
4.
J Vet Sci ; 24(1): e3, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-36560835

RESUMEN

BACKGROUND: Zinc (Zn) is an essential cofactor for physiological homeostasis in the body. Zn oxide (ZnO), an inorganic compound that supplies Zn, exists in various sizes, and its bioavailability may vary depending on the size in vivo. However, comparative studies on the nutritional effects of micro-sized ZnO (M-ZnO) and nano-sized ZnO (N-ZnO) supplementation on Zn deficiency (ZnD) animal models have not been reported. OBJECTIVES: This study investigated the nutritional bioavailability of N-ZnO and M-ZnO particles in dietary-induced ZnD mice. METHODS: Animals were divided into six experimental groups: normal group, ZnD control group, and four ZnO treatment groups (Nano-Low, Nano-High, Micro-Low, and Micro-High). After ZnD induction, N-ZnO or M-ZnO was administered orally every day for 4 weeks. RESULTS: ZnD-associated clinical signs almost disappeared 7 days after N-ZnO or M-ZnO administration. Serum Zn concentrations were higher in the Nano-High group than in the ZnD and M-ZnO groups on day 7 of ZnO treatment. In the liver and testis, Nano-Low and Nano-High groups showed significantly higher Zn concentrations than the other groups after 14-day treatment. ZnO supplementation increased Mt-1 mRNA expression in the liver and testis and Mt-2 mRNA expression in the liver. Based on hematoxylin-and-eosin staining results, N-ZnO supplementation alleviated histological damage induced by ZnD in the testis and liver. CONCLUSIONS: This study suggested that N-ZnO can be utilized faster than M-ZnO for nutritional restoration at the early stage of ZnD condition and presented Mt-1 as an indicator of Zn status in the serum, liver, and testis.


Asunto(s)
Óxido de Zinc , Animales , Masculino , Ratones , ARN Mensajero , Zinc/uso terapéutico , Óxido de Zinc/farmacología
5.
J Vet Sci ; 23(5): e74, 2022 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-36174978

RESUMEN

BACKGROUND: Previous studies have presented evidence to support the significant association between red meat intake and colon cancer, suggesting that heme iron plays a key role in colon carcinogenesis. Epigallocatechin-3-gallate (EGCG), the major constituent of green tea, exhibits anti-oxidative and anti-cancer effects. However, the effect of EGCG on red meat-associated colon carcinogenesis is not well understood. OBJECTIVES: We aimed to investigate the regulatory effects of hemin and EGCG on colon carcinogenesis and the underlying mechanism of action. METHODS: Hemin and EGCG were treated in Caco2 cells to perform the water-soluble tetrazolium salt-1 assay, lactate dehydrogenase release assay, reactive oxygen species (ROS) detection assay, real-time quantitative polymerase chain reaction and western blot. We investigated the regulatory effects of hemin and EGCG on an azoxymethane (AOM) and dextran sodium sulfate (DSS)-induced colon carcinogenesis mouse model. RESULTS: In Caco2 cells, hemin increased cell proliferation and the expression of cell cycle regulatory proteins, and ROS levels. EGCG suppressed hemin-induced cell proliferation and cell cycle regulatory protein expression as well as mitochondrial ROS accumulation. Hemin increased nuclear factor erythroid-2-related factor 2 (Nrf2) expression, but decreased Keap1 expression. EGCG enhanced hemin-induced Nrf2 and antioxidant gene expression. Nrf2 inhibitor reversed EGCG reduced cell proliferation and cell cycle regulatory protein expression. In AOM/DSS mice, hemin treatment induced hyperplastic changes in colon tissues, inhibited by EGCG supplementation. EGCG reduced the hemin-induced numbers of total aberrant crypts and malondialdehyde concentration in the AOM/DSS model. CONCLUSIONS: We demonstrated that EGCG reduced hemin-induced proliferation and colon carcinogenesis through Nrf2-inhibited mitochondrial ROS accumulation.


Asunto(s)
Factor 2 Relacionado con NF-E2 , Enfermedades de los Roedores , Animales , Antioxidantes , Azoximetano , Células CACO-2 , Carcinogénesis , Catequina/análogos & derivados , Proteínas de Ciclo Celular , Colon , Dextranos , Hemina/farmacología , Humanos , Hierro , Proteína 1 Asociada A ECH Tipo Kelch , Lactato Deshidrogenasas , Malondialdehído , Ratones , Especies Reactivas de Oxígeno , , Sales de Tetrazolio
6.
FEBS Open Bio ; 12(1): 71-81, 2022 01.
Artículo en Inglés | MEDLINE | ID: mdl-34431239

RESUMEN

Expanding on previous demonstrations of the therapeutic effects of adeno-associated virus (AAV) carrying small-hairpin RNA (shRNA) in downregulating the mechanistic target of rapamycin (mTOR) in in vivo retinal vascular disorders, vascular endothelial growth factor (VEGF)-stimulated endothelial cells were treated with AAV2-shmTOR to examine the role of mTOR inhibition in retinal angiogenesis. AAV2-shmTOR exposure significantly reduced mTOR expression in human umbilical vein endothelial cells (HUVECs) and decreased downstream signaling cascades of mTOR complex 1 (mTORC1) and mTORC2 under VEGF treatment. Moreover, the angiogenic potential of VEGF was significantly inhibited by AAV2-shmTOR, which preserved endothelial integrity by maintaining tight junctions between HUVECs. These data thus support previous in vivo studies and provide evidence that AAV2-shmTOR induces therapeutic effects by inhibiting the neovascularization of endothelial cells.


Asunto(s)
Dependovirus , Factor A de Crecimiento Endotelial Vascular , Dependovirus/genética , Dependovirus/metabolismo , Células Endoteliales de la Vena Umbilical Humana , Humanos , ARN Interferente Pequeño/farmacología , Serina-Treonina Quinasas TOR/metabolismo , Factor A de Crecimiento Endotelial Vascular/metabolismo
7.
Front Vet Sci ; 8: 650866, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34026890

RESUMEN

Feline parvovirus (FPV) is a small, non-enveloped, single-stranded DNA virus that infects cats. We recently isolated a feline parvovirus Fe-P2 strain from a dead stray cat in Iksan, 2017. Its partial genomic sequence (4,643 bases) was obtained, and phylogenetic analysis based on the VP2 nucleotide sequence showed that the FPV Fe-P2 strain was closely related to the FPV isolate Gigucheon in cat, 2017 (MN400978). In addition, we performed a serum neutralization (SN) test with the FPV isolates in various mammalian sera. These were from raccoon dog, water deer, Eurasian otter, Korean hare, leopard cat, and Asian badger, which were kindly provided by Chungnam Wild Animal Rescue Center. Notably, serological evidence of its infection was found in Asian badger, Meles leucurus (2/2) and leopard cat, Prionailurus bengalensis (5/8) through SN tests, whereas there was no evidence in raccoon dog, water deer, Eurasian otter, and Korean hare based on the collected sera in this study. These findings might provide partial evidence for the possible circulation of FPV or its related viruses among wild leopard cat and Asian badger in Korea. There should be additional study to confirm this through direct detection of FPVs in the related animal samples.

8.
Cells Dev ; 165: 203663, 2021 03.
Artículo en Inglés | MEDLINE | ID: mdl-33993984

RESUMEN

Asb2, ankyrin repeat, and SOCS box protein 2 form an E3 ubiquitin ligase complex. Asb2 ubiquitin ligase activity drives the degradation of filamins, which have essential functions in humans. The placenta is a temporary organ that forms during pregnancy, and normal placentation is important for survival and growth of the fetus. Recent studies have shown that approximately 25-30% of knockout (KO) mice have non-viable offspring, and 68% of knockout lines exhibit placental dysmorphologies. There are very few studies on Asb2, with insufficient research on its role in placental development. Therefore, we generated Asb2 knockout mice and undertook to investigate Asb2 expression during organogenesis, and to identify its role in early embryonic and placental development. The external morphology of KO embryos revealed abnormal phenotypes including growth retardation, pericardial effusion, pale color, and especially heart beat defect from E 9.5. Furthermore, Asb2 expression was observed in the heart from E 9.5, indicating that it is specifically expressed during early heart formation, resulting in embryonic lethality. Histological analysis of E 10.5 KO heart showed malformations such as failure of chamber formation, reduction in trabeculated myocardium length, absence of mesenchymal cells, and destruction of myocardium wall. Moreover, the histological results of Asb2-deficient placenta showed abnormal phenotypes including small labyrinth and reduced vascular complexity, indicating that failure to establish mature circulatory pattern affects the embryonic development and results in early mortality. Collectively, our results demonstrate that Asb2 knockout mice have placental defects, that subsequently result in failure to form a normal cardiac septum, and thereby result in embryo mortality in utero at around E 9.5.


Asunto(s)
Pérdida del Embrión/patología , Cardiopatías Congénitas/patología , Placenta/patología , Proteínas Supresoras de la Señalización de Citocinas/deficiencia , Alelos , Animales , Cruzamientos Genéticos , Pérdida del Embrión/genética , Embrión de Mamíferos/patología , Desarrollo Embrionario/genética , Femenino , Regulación del Desarrollo de la Expresión Génica , Marcación de Gen , Cardiopatías Congénitas/genética , Masculino , Ratones Noqueados , Fenotipo , Embarazo , Proteínas Supresoras de la Señalización de Citocinas/metabolismo
9.
Biomed Res Int ; 2020: 7957045, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32596374

RESUMEN

Identification of a new agent from natural products for the protection of embryonic anomalies is potentially valuable. To investigate the protective effect exerted by lycopene against nicotine-induced malformations, mouse embryos in embryonic day 8.5 with yolk sac placentas were cocultured with 1 mM nicotine and/or lycopene (1 × 10-6, 1 × 10-5 µM) for 48 h. The morphological defects and apoptotic cell deaths in the embryo and yolk sac placenta of the nicotine group were significantly increased. Exposure to nicotine resulted in reduced superoxide dismutase (SOD) activity and cytoplasmic SOD and cytoplasmic glutathione peroxidase mRNA levels, but increased lipid peroxidation level in embryos. Moreover, treatment with nicotine resulted in aggravated expressions of the mRNA or protein level of antiapoptotic (BCL2-associated X protein, B-cell lymphoma-extralarge, and caspase 3), anti-inflammatory (nuclear factor kappa-light-chain-enhancer of activated B cells and tumor necrosis factor-alpha), and vasculogenic (vascular endothelial growth factor-alpha, insulin-like growth factor-1, alpha smooth muscle actin, transforming growth factor-beta 1, and hypoxia inducible factor-1 alpha) factors in the embryo and yolk sac placenta. However, all the parameters were significantly improved by treatment with lycopene, as compared to the nicotine group. These findings indicate the potential of lycopene as a protective agent against embryonic anomalies and yolk sac vasculogenic and placenta-forming defects induced by nicotine through modulations of oxidative, apoptotic, vasculogenic, and inflammatory activities.


Asunto(s)
Embrión de Mamíferos/efectos de los fármacos , Licopeno/farmacología , Nicotina/toxicidad , Sustancias Protectoras/farmacología , Saco Vitelino/efectos de los fármacos , Animales , Apoptosis/efectos de los fármacos , Embrión de Mamíferos/patología , Femenino , Feto/efectos de los fármacos , Feto/patología , Inflamación/metabolismo , Ratones , Neovascularización Fisiológica/efectos de los fármacos , Placenta/efectos de los fármacos , Embarazo , Saco Vitelino/irrigación sanguínea , Saco Vitelino/patología
10.
Reprod Toxicol ; 91: 92-100, 2020 01.
Artículo en Inglés | MEDLINE | ID: mdl-31756439

RESUMEN

Pregnant women drink caffeinated beverages using bisphenol A (BPA)-coated cans without knowing the potential risks. In this study, mouse embryos (embryonic day 8.5) surrounded by yolk sac placenta were cultured with caffeine (30, 60, and 120 µg/ml) and/or BPA (35 µg/ml) for 48 h. In response to a single administration of BPA or caffeine dose, embryonic development was similar to normal control embryos. However, the combined exposure to caffeine and BPA dose-dependently increased embryonic anomalies, and thinner ventricular wall and trabeculae disorders of heart were observed. The mRNA levels of various anti-oxidative, apoptotic, and hypoxic genes were significantly altered in the treated embryos. Furthermore, abnormal vasculogenesis, reduced vasculogenic growth factor expressions, and apoptotic cell death were detected in yolk sac placentas. These findings indicate that the combined exposure to caffeine and BPA induces embryonic anomalies and injuries of the yolk sac placentas through oxidative stress, apoptosis, hypoxia, and vasculogenic defects.


Asunto(s)
Compuestos de Bencidrilo/toxicidad , Cafeína/toxicidad , Embrión de Mamíferos/efectos de los fármacos , Fenoles/toxicidad , Animales , Apoptosis/efectos de los fármacos , Desarrollo Embrionario/efectos de los fármacos , Femenino , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Hipoxia/inducido químicamente , Masculino , Ratones , Ratones Endogámicos ICR , Estrés Oxidativo/efectos de los fármacos , Placenta , Embarazo , Ratas Sprague-Dawley , Saco Vitelino
11.
Lab Anim Res ; 34(3): 132, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-30310410

RESUMEN

[This corrects the article on p. 37 in vol. 34, PMID: 29628975.].

12.
Lab Anim Res ; 34(1): 30-36, 2018 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-29628974

RESUMEN

This study investigated the anti-cancer potential of a near-infrared fluorescence (NIRF) molecule conjugated with Cetuximab (Cetuximab-NIRF) in six-week-old female BALB/c athymic (nu+/nu+) nude mice. A431 cells were cultured and injected into the animals to induce solid tumors. Paclitaxel (30 mg/kg body weight (BW)), Cetuximab (1 mg/kg BW), and Cetuximab-NIRF (0.25, 0.5 and 1.0 mg/kg BW) were intraperitoneally injected twice a week into the A431 cell xenografts of the nude mice. Changes in BW, tumor volume and weight, fat and lean mass, and diameter of the peri-tumoral blood vessel were determined after two weeks. Tumor volumes and weights were significantly decreased in the Cetuximab-NIRF (1 mg/kg BW) group compared with the control group (P<0.001). Lean mass and total body water content were also conspicuously reduced in the Cetuximab-NIRF (1 mg/kg BW) group compared with the vehicle control group. Peri-tumoral blood vessel diameters were very thin in the Cetuximab-NIRF groups compared with those of the paclitaxel group. These results indicate that the conjugation of Cetuximab with NIRF does not affect the anti-cancer potential of Cetuximab and NIRF can be used for molecular imaging in cancer treatments.

13.
Lab Anim Res ; 34(1): 37-43, 2018 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-29628975

RESUMEN

This study was performed to investigate the effect of a concentrate of fermented wild ginseng root culture (HLJG0701) on memory improvement in the scopolamine (SPL)-induced memory-deficient mouse model. Eight-week-old male ICR mice were used to evaluate the protective effect of HLJG0701 against the SPL-induced memory loss animal model. The Morris water maze test, which measures hippocampus-dependent learning ability, and the Y-maze test, a short-term memory assessment test, were performed and related markers were analyzed. HLJG0701-treated groups displayed significantly reduced acetylcholinesterase activity and increased acetylcholine level compared with the SPL-administered group (SPL-G) (P<0.05). In the Y-maze test, the spontaneous alternation in al HLJG0711-treated groups was significantly increased compared with that in SPL-G (P<0.05). In the Morris water maze test, the escape latency and time spent in the target quadrant in all HLJG0701-treated groups were significantly decreased and increased, respectively, compared with those in SPL-G (P<0.05). In addition, the brain-derived neurotrophic factor level in groups treated with HLJG0701 300 and 600 mg/kg body weight was significantly increased compared with that in SPL-G (P<0.05). These results suggest that the HLJG0701 may protect against memory loss by inhibiting acetylcholinesterase activity and preventing acetylcholine deficiency.

14.
Lab Anim Res ; 34(4): 329, 2018 12.
Artículo en Inglés | MEDLINE | ID: mdl-30671122

RESUMEN

[This retracts the article on p. 37 in vol. 34, PMID: 29628975.].

15.
J Med Imaging Radiat Oncol ; 61(4): 476-480, 2017 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-28105788

RESUMEN

INTRODUCTION: The aims of this study were to investigate the diagnostic performance of computed tomography colonography (CTC) performed in a rural secondary hospital, and to describe the local pattern of CTC service provision. METHOD: A single site, retrospective observational analysis was conducted for all patients undergoing CTC during the 12-month period from 1st of January to 31st of December 2014 with comparison to available colonoscopy. RESULTS: There were 639 CTCs performed during the 12-months period. The average time from referral to performance of CTC scan was 21.3 days. The diagnostic yield of CTC for CRC was 5.8%; and for large polyps ≥10 mm was 8.0%. The sensitivity and specificity of CTC for detecting CRC were 97.1% and 88.2% respectively. The most predictive symptoms for finding colorectal lesions were rectal bleeding and anaemia. The referral rate from CTC to colonoscopy was 16.9%. 63 patients (9.9%) had follow up recommendations made in their reports due to extracolonic findings. CONCLUSION: Computed tomography colonography performed in a rural secondary hospital provided sufficient sensitivity to detect large polyps or CRC. The specificity for CRC was lower than reported figures in the literature. Technical issue of CTC performance due to poor insufflation techniques was identified as a main contributing factor reducing CTC accuracy. CTCs were performed with acceptable waiting time and showed high overall diagnostic yield for colorectal neoplasm in a rural hospital.


Asunto(s)
Colonografía Tomográfica Computarizada , Neoplasias Colorrectales/diagnóstico por imagen , Anciano , Colonoscopía , Femenino , Hospitales Rurales , Humanos , Masculino , Valor Predictivo de las Pruebas , Estudios Retrospectivos , Sensibilidad y Especificidad
16.
Biomed Res Int ; 2016: 2572073, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-28050551

RESUMEN

Scrotal hyperthermia leads to oxidative stress and apoptosis in spermatogenic cells, which subsequently causes male infertility. In this study, we examined the effects of ß-carotene and/or curcumin on heat-stress- (HS-) induced testicular injuries in mice. ICR male mice (8 weeks old) were consecutively treated with ß-carotene (10 mg/kg) and/or curcumin (20 mg/kg) orally once a day for 14 days and then subjected to single exposure with scrotal HS at 43°C for 15 min on day 7. HS induced a significant reduction in testicular weight, appearance of multinucleated giant cells, and desquamation of germ cells in destructive seminiferous tubules, as well as degenerative Leydig cells. Moreover, HS reduced the superoxide dismutase (SOD) activity and mRNA levels of mitochondrial SOD, phospholipid hydroperoxide glutathione peroxidase, B-cell lymphoma-extra-large, and 3ß-hydroxysteroid dehydrogenase, with increases in lipid peroxidation levels and mRNA levels of BCL2-associated X protein and caspase-3 relative to those of the control group. However, these changes were significantly recovered by combined treatment with ß-carotene and curcumin after HS. These findings indicate that the combined treatment with ß-carotene and curcumin might be a valuable protective agent to ameliorate hyperthermic spermatogenic disorders via its potent antioxidative, antiapoptotic, and androgen synthetic effects.


Asunto(s)
Carotenoides/farmacología , Curcumina/farmacología , Hipertermia Inducida , Sustancias Protectoras/farmacología , Espermatogénesis/efectos de los fármacos , 3-Hidroxiesteroide Deshidrogenasas/metabolismo , Animales , Antioxidantes/metabolismo , Apoptosis/efectos de los fármacos , Apoptosis/genética , Regulación de la Expresión Génica/efectos de los fármacos , Respuesta al Choque Térmico/efectos de los fármacos , Peroxidación de Lípido/efectos de los fármacos , Masculino , Ratones Endogámicos ICR , Tamaño de los Órganos/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , ARN Mensajero/genética , ARN Mensajero/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Superóxido Dismutasa/metabolismo , Testículo/efectos de los fármacos , Testículo/enzimología , Testículo/patología
17.
Reprod Toxicol ; 57: 73-80, 2015 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-26050606

RESUMEN

Severe oxidative stress by cryptorchidism leads to infertility. To assess the functional significance of phospholipid hydroperoxidase glutathione peroxidase (PHGPx) under cryptorchidism, PHGPx expression was spatiotemporally analyzed in testes and epididymis excised at 1, 4, 7, 14, 21, and 28 days after experimental bilateral cryptorchidism in adult mice. In testes, while apoptosis-related caspase 3 and Bcl-xL mRNAs were significantly changed after 14 days, 3 beta-hydroxysteroid dehydrogenase mRNA was greatly reduced immediately after cryptorchidism. Under cryptorchidism, PHGPx was significantly decreased in both organs after 21 days, while its mRNA was greatly reduced in testes after 14 days and in epididymis after 4 days. However, PHGPx was upregulated in degenerative spermatids, multinucleated giant cells, and Leydig cells in testes and desquamous spermatids in epididymis until 21 days, but was weakly detected in the spermatids at 28 days. These findings suggest that PHGPx is necessary for maintenance of male fertility under cryptorchidism in testes.


Asunto(s)
Criptorquidismo/metabolismo , Epidídimo/metabolismo , Fertilidad/fisiología , Glutatión Peroxidasa/metabolismo , Testículo/metabolismo , 3-Hidroxiesteroide Deshidrogenasas/genética , Animales , Caspasa 3/genética , Criptorquidismo/patología , Epidídimo/patología , Masculino , Ratones Endogámicos ICR , Fosfolípido Hidroperóxido Glutatión Peroxidasa , ARN Mensajero/metabolismo , Testículo/patología , Proteína bcl-X/genética , Glutatión Peroxidasa GPX1
18.
J Prosthet Dent ; 113(4): 329-35, 2015 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25681353

RESUMEN

STATEMENT OF PROBLEM: A recently introduced presintered cobalt-chromium (Co-Cr) alloy for metal ceramic restorations can be efficiently processed with computer-aided design/computer-aided manufacturing (CAD/CAM) techniques. However, little or no reliable study data are available regarding the bonding ability of porcelain to milled Co-Cr alloys. PURPOSE: The purpose of this study was to evaluate the shear bond strength of veneering porcelain to the presintered Co-Cr alloy and to a conventional castable alloy. MATERIAL AND METHODS: Ninety-six cylindrical cores (6.8 mm in diameter, 9 mm in height) were made of millable alloy (Ceramill Sintron) and castable alloy (4-all) by means of CAD/CAM or casting, 48 cores for each alloy. Four types of veneering porcelain were fired or pressed to the cores; these specimens had dimensions of 4×4×3 mm. After firing, the specimens were put in resin molds, fixed in a universal testing machine, and subjected to a shear force test. Loading was applied to each specimen through the attached crosshead at a constant drive speed of 0.5 mm/min until fracture occurred. Shear bond strengths (MPa) were calculated by dividing the maximum failure force over the cross-sectional area of each specimen. Failure patterns of the specimens were also investigated and characterized as adhesive, cohesive, or mixed. One-way ANOVA and the Duncan post hoc test were used to analyze statistically significant differences between groups (α=.05). RESULTS: The means of the shear bond strengths of (millable) Ceramill Sintron were similar to or higher than those of (castable) 4-all cores. The shear bond strength was significantly lower for Press-To-Metal veneer than for the other fired veneers in the test (P<.001). The pattern of failure in most specimens was mixed, except for Press-To-Metal veneer, where cohesive failure occurred. CONCLUSIONS: The bonding ability of the traditional castable alloy was similar to that of the presintered millable Co-Cr alloy.


Asunto(s)
Aleaciones de Cromo/química , Diseño Asistido por Computadora , Recubrimiento Dental Adhesivo , Revestimiento para Colado Dental/química , Porcelana Dental/química , Adhesividad , Óxido de Aluminio/química , Grabado Dental/métodos , Análisis del Estrés Dental/instrumentación , Coronas con Frente Estético , Calor , Humanos , Ensayo de Materiales , Aleaciones de Cerámica y Metal/química , Microscopía Electrónica de Rastreo , Resistencia al Corte , Propiedades de Superficie
19.
J Surg Case Rep ; 2015(1)2015 Jan 18.
Artículo en Inglés | MEDLINE | ID: mdl-25600131

RESUMEN

Jejunal diverticula are rare and the condition remains mostly asymptomatic. However, they can present with vague chronic abdominal symptoms and, in some cases, acute life-threatening complications, such as gastrointestinal (GI) bleeding, bowel obstruction and perforation. We present a case of an adult male who presented with life-threatening GI bleeding secondary to jejunal diverticular disease. Whilst there are undoubtedly more common causes of GI bleeding, this case demonstrates that jejunal diverticular disease should remain on the differential diagnosis and investigations to confirm the diagnosis should be considered. However, despite investigations, the diagnosis may remain elusive and in patients with on-going bleeding, laparotomy and surgical resection is currently the treatment of choice.

20.
J Geriatr Cardiol ; 11(1): 83-9, 2014 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-24748887

RESUMEN

Several studies have shown that coronary artery bypass graft surgery (CABG) is superior to percutaneous coronary intervention (PCI) in patients with diabetes and multi-vessel disease. Whether this advantage of CABG over PCI is confined to diabetics who require insulin is unknown. We review the published literature comparing CABG with PCI in diabetics including 8 cohorts and 4,786 patients. There was a lower rate for all-cause mortality (Relative risk (RR): 0.78, 95% confidence interval (CI): 0.62-0.99), and for major adverse cardiac and cerebrovascular events (MACCE, RR: 0.59, 95% CI: 0.47-0.75) for CABG compared to PCI. Composite outcome of mortality, myocardial infarction and stoke was similar between CABG and PCI (RR: 0.87, 95% CI: 0.54-1.42). Visual inspection of the forest plots showed that in most analyses, the point estimates of the RR are similar between the insulin requiring group and non-insulin requiring group. On meta-regression, there was no interaction between status of insulin requirement and revascularization strategies (P > 0.05 for all). The presented data on the still unpublished analysis of the FREEDOM trial showed similar results. Thus, in the current era, CABG is superior to PCI with lower mortality and MACCE rates, but the state of insulin requirement had no effect on the outcomes from the two revascularization strategies.

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