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Tissue Cell ; 39(3): 161-9, 2007 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-17412380

RESUMEN

We examined the participation of MAPK and PKA in the Golgi complex disassembly caused by light-activated Calphostin C in HT-29 cells. When these cells were incubated with Calphostin C, fragmentation and dispersal of the Golgi complex was observed as assessed by immunofluorescence microscopy. Electron microscopy analysis showed that clusters of vesicles and large tubule-vesicular membrane structures, resembling the Golgi remnants present in mitotic cells, substituted the Golgi stacks. In addition, Calphostin C treatment caused inhibition of the endocytic route. We confirmed that the Golgi disassembly was not due to PKC inhibition, and suggested, based on the use of specific inhibitors, that other kinases are involved. It was shown that pretreatment with PD98059 and H-89, both inhibitors of MAPK and PKA, respectively, prior to incubation with Calphostin C, caused blockade of the Golgi disassembly, as well as the inhibition of the endocytic pathway caused by this drug. This finding supports the existence of a novel mechanism by which MAPK and PKA may regulate the Golgi breakdown caused by Calphostin C in HT-29 cells.


Asunto(s)
Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Aparato de Golgi/metabolismo , Aparato de Golgi/efectos de la radiación , Luz , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Naftalenos/farmacología , Naftalenos/efectos de la radiación , Endocitosis/efectos de los fármacos , Endocitosis/efectos de la radiación , Flavonoides/farmacología , Técnica del Anticuerpo Fluorescente , Aparato de Golgi/efectos de los fármacos , Aparato de Golgi/ultraestructura , Células HT29 , Peroxidasa de Rábano Silvestre/metabolismo , Humanos , Isoquinolinas/farmacología , Naftalenos/química , Estaurosporina/farmacología , Sulfonamidas/farmacología
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