Bacterial mineralization of chromium-copper spinel with highly performance in electroplating effluent.

Cr (VI) contamination has posed severe challenges to water quality, food safety, and land resources. Microbial reduction of Cr(VI) to Cr(III) has drawn considerable attention due to its low cost and environmental friendliness. However, recent reports have shown that Cr(VI) generates highly migratable organo-Cr(III) rather than stable inorganic chromium minerals during the biological reduction process. In this work, it was reported for the first time that spinel structure CuCr2O4 was formed by Bacillus cereus in Cr biomineralization process. Different from known biomineralization models (biologically controlled mineralization and biologically induced mineralization), the chromium-copper minerals here appeared as specialized minerals with extracellular distribution. In view of this, a possible mechanism of biologically secretory mineralization was proposed. In addition, Bacillus cereus demonstrated a high conversion ability in the treatment of electroplating wastewater. The Cr(VI) removal percentage reached 99.7%, which satisfied the Chinese emission standard of pollutants for electroplating (GB 21,900-2008), indicating its application potential. Altogether, our work elucidated a bacterial chromium spinel mineralization pathway and evaluated the potential of this system for application in actual wastewater, opening a new avenue in the field of chromium pollution treatment and control.

Metformin-Induced Epigenetic Toxicity in Zebrafish: Experimental and Molecular Dynamics Simulation Studies.

The increased detection of many prescription drugs in aquatic environments has heightened concerns of their potential ecotoxicological effects. In this study, the effects of metformin (MEF) exposure on tissue accumulation, gene expression, and global DNA methylation (GDM) in zebrafish were investigated. The toxic mechanism of MEF exposure was simulated by molecular dynamics (MD) to reveal any conformational changes to DNA methyltransferase 1 (DNMT1). The results showed MEF accumulation in the gills, gut, and liver of zebrafish after 30 days of exposure, and the bioaccumulation capacity was in the order of gut > liver > gills. After a 30 day recovery period, MEF could still be detected in zebrafish tissues in groups exposed to MEF concentrations ≥ 10 µg/L. Moreover, the liver was the main site of GDM, and the restoration of GDM in the liver was slower than that in the gut and gills during the recovery period. Furthermore, MEF could induce the abnormal expression of CYP3A65, GSTM1, p53, and DNMT1 genes in the liver due to the formation of hydrogen bonds between MEF and the protein residues of those genes. The MD simulation allowed for the mechanistic determination of MEF-induced three-dimensional (3D) conformational changes and changes to the catalytic activity of DNMT1.