Emerging functions and therapeutic targets of IL-38 in central nervous system diseases.

Interleukin (IL)-38 is a newly discovered cytokine of the IL-1 family, which binds various receptors (i.e., IL-36R, IL-1 receptor accessory protein-like 1, and IL-1R1) in the central nervous system (CNS). The hallmark physiological function of IL-38 is competitive binding to IL-36R, as does the IL-36R antagonist. Emerging research has shown that IL-38 is abnormally expressed in the serum and brain tissue of patients with ischemic stroke (IS) and autism spectrum disorder (ASD), suggesting that IL-38 may play an important role in neurological diseases. Important advances include that IL-38 alleviates neuromyelitis optica disorder (NMOD) by inhibiting Th17 expression, improves IS by protecting against atherosclerosis via regulating immune cells and inflammation, and reduces IL-1ß and CXCL8 release through inhibiting human microglial activity post-ASD. In contrast, IL-38 mRNA is markedly increased and is mainly expressed in phagocytes in spinal cord injury (SCI). IL-38 ablation attenuated SCI by reducing immune cell infiltration. However, the effect and underlying mechanism of IL-38 in CNS diseases remain inadequately characterized. In this review, we summarize the biological characteristics, pathophysiological role, and potential mechanisms of IL-38 in CNS diseases (e.g., NMOD, Alzheimer's disease, ASD, IS, TBI, and SCI), aiming to explore the therapeutic potential of IL-38 in the prevention and treatment of CNS diseases.

Exploring NK-Cell molecules that impact the immune response and microenvironment in head and neck squamous cell carcinoma.

NK cells play a role in various cancers, but their role in head and neck squamous cell carcinoma (HNSCC) still needs to be explored. All public data are obtained from the Cancer Genome Atlas Program (TCGA) database. All analysis was performed using specific packages in R software. In our study, we quantified the immune microenvironment of HNSCC through multiple algorithms. Next, we identified NK cell-associated genes by quantifying NK cells, including SSNA1, TRIR, PAXX, DPP7, WDR34, EZR, PHLDA1 and ELOVL1. Then, we explored the single-cell expression pattern of these genes in the HNSCC microenvironment. Univariate Cox regression analysis indicated that the EZR, PHLDA1 and ELOVL1 were related to the prognosis of HNSCC patients. Following this, we selected EZR for further analysis. Our results showed that the patients with high EZR expression might have a poor prognosis and worse clinical features. Biological enrichment analysis showed that EZR is associated with many oncogenic pathways and a higher tumour stemness index. Meanwhile, we found that EZR can remodel the immune microenvironment of HNSCC. Moreover, we noticed that EZR could affect the immunotherapy and specific drug sensitivity, making it an underlying clinical target. In summary, our results can improve the understanding of NK cell in HNSCC. Meanwhile, we identified EZR as the underlying clinical target of HNSCC.

An integrated approach of machine learning and Bayesian spatial Poisson model for large-scale real-time traffic conflict prediction.

The use of traffic conflicts in road safety evaluation is gaining considerable popularity as it plays a vital role in developing a proactive safety management strategy and allowing for real-time safety analysis. This study proposes an integrated approach that combines a machine learning (ML) algorithm and a Bayesian spatial Poisson (BSP) model to conduct large-scale real-time traffic conflict prediction by considering traffic states as the explanatory variables. Traffic conflicts are measured by two indicators, the Time to Collision (TTC) and the Post-Encroachment Time (PET). Based on both TTC and PET, traffic conflict severity is classified into five categories. For each conflict severity category, a binary variable (conflict occurrence) and a count variable (conflict frequency) are developed, respectively. In addition to conflict variables, traffic state parameters are extracted from a large-scale high-resolution trajectory dataset. The traffic parameters include volume, density, speed, and the corresponding space-based and space-time-based measures within a 30-second interval. Eight ML-based classifiers are applied to predict conflict occurrence, and the best classifier is selected. A binary logistic regression is developed to explore the potential linkages between traffic states and conflict occurrence. As well, a resampling technique Borderline-SMOTE is used to mitigate the sparsity caused by the predefined short interval. The BSP model is utilized to predict the specific number of conflicts. Further, the BSP model can also explain the relationship between traffic states and conflict frequency, and thus the significant influencing traffic states are identified. The results show that random forest outperforms the other MLs in terms of conflict occurrence prediction accuracy. Further, the proposed integrated approach achieves a high performance of conflict frequency prediction with RMSE values of 0.1384 âˆ¼ 0.1699, MAPE values of 9.25% ∼ 36.99%, and MAE values of 0.0087 âˆ¼ 0.6398. The finding emphasizes the need for separately predicting the occurrence and frequency of conflicts with different severities.

Effect of adipose tissue-derived stem cells therapy on clinical response in patients with primary Sjogren's syndrome.

The purpose of this trial was to clinically assess the effect and safety of Adipose Tissue-derived Stem Cells (ADSCs) treatment on primary Sjogren's Syndrome (pSS). In this 6-month randomized, triple-blind, placebo-controlled clinical trial, pSS patients were randomly assigned to two groups. After demographic characteristics and clinical examination were achieved, local injection of ADSCs into bilateral glands was performed with patients in ADSCs group (n = 35) and placebo solution was used for another group (n = 39) at three time points. Patients were followed up at 1-, 3- and 6-month. At each visit, studies of clinical and laboratory outcomes, as well as subjective symptoms, were conducted. A total of 74 subjects who met the including criteria were allocated in two groups and eventually 64 subjects (86.5%) completed the treatments and the follow-up assessments. Secretion of salivary and lachrymal glands were significantly improved in 3-month (P < 0.05). A great improvement of European League Against Rheumatism Sjögren's Syndrome Disease Activity Index (ESSDAI) was found after ADSCs treatment with intergroup comparison from baseline to follow-up (P < 0.05). There is also a significant difference of European Alliance of Associations for Rheumatology SS Patient Reported Index (ESSPRI) between the two groups in the follow-up (P < 0.05). A significant abatement of IgG, IgM, C3, C4 and ESR between two groups was observed in part of follow-up time points (P < 0.05). The ADSCs therapy can provide relief of oral and eye's dryness in our trial in a short time and has potential improvement of subjective and systemic syndromes of pSS.

Extended characterization of IL-33/ST2 as a predictor for wound age determination in skin wound tissue samples of humans and mice.

Interleukin (IL)-33, an important inflammatory cytokine, is highly expressed in skin wound tissue and serum of humans and mice, and plays an essential role in the process of skin wound healing (SWH) dependent on the IL-33/suppression of tumorigenicity 2 (ST2) pathway. However, whether IL-33 and ST2 themselves, as well as their interaction, can be applied for skin wound age determination in forensic practice remains incompletely characterized. Human skin samples with injured intervals of a few minutes to 24 hours (hs) and mouse skin samples with injured intervals of 1 h to 14 days (ds) were collected. Herein, the results demonstrated that IL-33 and ST2 are increased in the human skin wounds, and that in mice skin wounds, there is an increase over time, with IL-33 expression peaking at 24 hs and 10 ds, and ST2 expression peaking at 12 hs and 7 ds. Notably, the relative quantity of IL-33 and ST2 proteins < 0.35 suggested a wound age of 3 hs; their relative quantity > 1.0 suggested a wound age of 24 hs post-mouse skin wounds. In addition, immunofluorescent staining results showed that IL-33 and ST2 were consistently expressed in the cytoplasm of F4/80-positive macrophages and CD31-positive vascular endothelial cells with or without skin wounds, whereas nuclear localization of IL-33 was absent in α-SMA-positive myofibroblasts with skin wounds. Interestingly, IL-33 administration facilitated the wound area closure by increasing the proliferation of cytokeratin (K) 14 -positive keratinocytes and vimentin-positive fibroblasts. In contrast, treating with its antagonist (i.e., anti-IL-33) or receptor antagonist (e.g., anti-ST2) exacerbated the aforementioned pathological changes. Moreover, treatment with IL-33 combined with anti-IL-33 or anti-ST2 reversed the effect of IL-33 on facilitating skin wound closure, suggesting that IL-33 administration facilitated skin wound closure through the IL-33/ST2 signaling pathway. Collectively, these findings indicate that the detection of IL-33/ST2 might be a reliable biomarker for the determination of skin wound age in forensic practice.

Prevalence of incidental thyroid abnormalities in patients with degenerative cervical spondylosis: a retrospective cross-sectional magnetic resonance imaging study.

Background: Incidental thyroid abnormalities found on magnetic resonance imaging (MRI) of the neck are not uncommon. This study aimed to investigate the prevalence of incidental thyroid abnormalities in the cervical spine MRI of the degenerative cervical spondylosis (DCS) population indicated for surgery and to identify patients who require additional workup based on the recommendations of the American College of Radiology (ACR). Methods: All consecutive patients with DCS and indications for cervical spine surgery from October 2014 to May 2019 in the Affiliated Hospital of Xuzhou Medical University were reviewed. All MRI scans of the cervical spine routinely include the thyroid. Cervical spine MRI scans were retrospectively evaluated for the prevalence, size, morphologic characteristics, and location of incidental thyroid abnormalities. Results: A total of 1,313 patients were included in the analysis, 98 (7.5%) of whom were found to have incidental thyroid abnormalities. The most frequent thyroid abnormality was thyroid nodules (5.3%), followed by goiters (1.4%). Other thyroid abnormalities included Hashimoto thyroiditis (0.4%) and thyroid cancer (0.5%). There was a statistically significant difference in age and sex between patients with DCS with and without incidental thyroid abnormalities (P=0.018 and P=0.007). Stratified by age, the results showed that the highest incidence of incidental thyroid abnormalities was found in patients aged 71 to 80 years (12.4%). Eighteen patients (1.4%) needed further ultrasound (US) and relevant workups. Conclusions: Incidental thyroid abnormalities are common in cervical MRI, with a prevalence of 7.5% identified in patients with DCS. Incidental thyroid abnormalities are large or have suspicious imaging features, and further evaluation with a dedicated thyroid US examination should be completed before cervical spine surgery is undertaken.

Experimental Research and Numerical Simulation of Laser Welding of 303Cu/440C-Nb Stainless-Steel Thin-Walled Natural-Gas Injector for Vehicles.

This paper presents the results of research on laser lap welding technology of heterogeneous materials and a laser post-heat treatment method to enhance welding performance. The purpose of this study is to reveal the welding principle of austenitic/martensitic dissimilar stainless-steel materials (3030Cu/440C-Nb) and to further obtain welded joints with good mechanical and sealing properties. A natural-gas injector valve is taken as the study case where its valve pipe (303Cu) and valve seat (440C-Nb) are welded. Experiments and numerical simulations were conducted where the welded joints' temperature and stress fields, microstructure, element distribution, and microhardness were studied. The results showed that the residual equivalent stresses and uneven fusion zone tend to concentrate at the joint of two materials within the welded joint. The hardness of the 303Cu side (181.8 HV) is less than the 440C-Nb side (266 HV) in the center of the welded joint. The laser post-heat treatment can reduce the residual equivalent stress in the welded joint and improve the mechanical and sealing properties. The results of the press-off force test and the helium leakage test showed that the press-off force increased from 9640 N to 10,046 N and the helium leakage rate decreased from 3.34 × 10-4 to 3.96 × 10-6.

Regulating Interlayer Confinement FeOCl for Accelerating Polymerization of Pollutants to Reduce Carbon Emission in Water Purification.

The spatial structure regulation of catalysts could optimize the reaction pathway and enhance the mass transfer kinetics, which might realize the efficient and low-consumption removal of pollutants in Fenton-like technology. In this study, N,N-dimethylformamide (DMF) intercalation was used to adjust the interlayer spacing of FeOCl from 7.90 to 11.84 Å by a simple and rapid intercalation method, thereby enhancing the mass transfer kinetics and altering the catalytic pathway. The removal rate of BPA in the DMF-FeOCl/PS system increased by 8.78 times, showing good resistance to complex water environments (such as pH, humic acid, and anions), especially in 5 g/L high-salt wastewater. The direct electron transfer processes between Fe(IV) and pollutants mediated by interlayer Fe sites generate phenoxy radicals, and the polymerization processes occur, achieving efficient removal of pollutants and low CO2 emissions. This study provides new insight into the efficient and low-carbon treatment of high-salt wastewater.

Revealing the synergistic mechanism of the generation, migration and nearby utilization of reactive oxygen species in FeOCl-MOF yolk-shell reactors.

Fenton-like reactions is attractive for environmental pollutant control, but there is an urgent need to improve the utilisation of hydroxyl radicals (·OH) in practical applications. Here, for the first time, FeOCl is encapsulated within a Metal Organic Framework (MOF) (Materials of Institut Lavoisier-101 (MIL-101(Fe))) as a yolk-shell reactor (FeOCl-MOF) by in situ growth. The interaction between FeOCl and the MOF not only increases the electron density of FeOCl, but also shifts down the d-band centre. The increase of electron density could promote the efficient conversion of H2O2 to ·OH catalysed by FeOCl. And the shift of the d-band centre to the lower energy level facilitates the desorption of ·OH. Experimental and theoretical calculations showed that the high catalytic performance was attributed to the unique yolk-shell structure that concentrates the catalytic and adsorption sites in a confinement space, as well as the improved electron density and d-band centre for efficient generation, rapid desorption and utilized nearby of ·OH. Which is utilized nearby by the organic pollutants adsorbed by the surface MOF, thus greatly improving the effective conversion of H2O2 and the ·OH utilisation (from 25.5% (Fe2+/H2O2) to 77.1% (FeOCl-MOF/H2O2)). In addition, a catalytic reactor was constructed to achieve continuous efficient treatment of organic pollutants. This work provides a Fenton-like microreactor for efficient generation, rapid desorption, and nearby utilization of ·OH to improve future technologies for deep water purification in complex environments.

Ultrafast short-range catalytic pathway modified peroxymonosulfate activation over CuO with surface oxygen defects for tetracycline hydrochloride degradation.

The presence of antibiotics in water bodies seriously threatens the ecosystem and human health. Advanced oxidation processes (AOPs) based on peroxymonosulfate (PMS), an effective method to remove antibiotics, have a bottleneck problem that the low oxidant utilization is attributed to the hindered electron transfer between metal oxides and peroxides. Here, CuO with rich oxygen vacancies (OVs), MSCuO-300, was synthesized to efficiently degrade tetracycline hydrochloride (TTCH) (k = 0.095 min-1). The dominant role of direct adsorption and activation of OVs and its regulated Cu-O, rather than surface hydroxyl adsorption, mediated a short-range catalytic pathway. The shortened catalytic pathway between active sites and PMS accelerated the charge transfer at the interface, which promoted PMS activation. Compared with CuxO-500 and Commercial CuO, the activation rate of PMS was increased by 11.97, and 12.64 times, respectively. OVs contributed to the production of 1O2 and O2•-, the main active species. In addition, MSCuO-300/PMS showed excellent adaptability to real water parameters, such as pH (3-11), anions, and continuous reactor maintained for 168 h. This study provides a successful case for the purification of antibiotic-containing wastewater in the design of efficient catalysts by oxygen defect strategies.

Nanoconfinement-enhanced Fenton-like polymerization via hollow hetero-shell carbon for reducing carbon emissions in organic wastewater purification.

Lower reaction speed and excessive oxidant inputs impede the removal of contaminants from water via the advanced oxidation processes based on peroxymonosulfate. Herein, we report a new confined catalysis paradigm via the hollow hetero-shell structured CN@C (H-CN@C), which permits effective decontamination through polymerization with faster reaction rates and lower oxidant dosage. The confined space structures regulated the CN and CO and electron density of the inner shell, which increased the electron transfer rate and mass transfer rate. As a result, CN in H-CN@C-10 reacted with peroxymonosulfate in preference to CO to generate singlet oxygen, improving the second-order reaction kinetics by 503 times. The identification of oxidation products implied that bisphenol AF could effectively remove by polymerization, which could reduce carbon dioxide emissions. These favorable properties make the nanoconfined catalytic polymerization of contaminants a remarkably promising nanocatalytic water purification technology.

Should blastocyst transfer be performed in patients with 1-3 embryos available on day 3?

RESEARCH QUESTION: In patients with 1-3 embryos available on day 3, does blastocyst transfer reduce the chances of a clinical pregnancy by cancelling transfer cycles compared with cleavage transfer? DESIGN: This retrospective observational study included 423 IVF cycles performed from 1 January 2019 to 31 December 2020 at the Center for Reproduction and Fertility of the Second Affiliated Hospital of Kunming Medical University. Cleavage transfer was performed in 267 cycles and blastocyst transfer was performed in 156 cycles. The primary outcome was the ongoing pregnancy rate, and the secondary outcomes were clinical pregnancy rate and embryo cessation rate. Univariate analysis was performed to compare outcomes. A logistic regression analysis was performed to explore the association between transfer stage and ongoing pregnancy rate. RESULTS: No differences were observed in the ongoing pregnancy rate (25.84% versus 26.92%; odds ratio [OR] 0.95; 95% confidence interval [CI] 0.61-1.50; P = 0.82) and embryo cessation rate (83.48% versus 85.75%; OR 1.19; 95% CI 0.82-1.75; P = 0.40) between the two groups. Logistic regression analysis showed no association between transfer stage and ongoing pregnancy rate (OR 1.06; 95% CI 0.64-1.73). CONCLUSIONS: Blastocyst transfer does not reduce the chances of a clinical pregnancy. These results support the proposal of blastocyst transfer in patients with 1-3 embryos available on day 3.

Fe-O-Zr in MOF for effective photo-Fenton Bisphenol A degradation: Boosting mechanism of electronic transmission.

To enhance the efficiency of photogenerated electron transport in the photo-Fenton reaction, we report a Fe-doped UiO-66 containing Fe-O-Zr bonds for the photo-Fenton reaction system. The modulation changes the energy bandgap from 3.89 eV to 2.02 eV, and its absorption edge is red-shifted from the UV region to the visible range. Simultaneously, Fe-O-Zr reduces the redox internal resistance, enhances the photocurrent and catalytic process, and suppresses the compounding of photogenerated electrons and holes. These promote the valence cycling of Fe(III)/Fe(II) in the photo-Fenton reaction. Compared with UiO-66, the hydroxyl radical generation efficiency of this reaction system was increased by 5.8 times (UiO-66: 0.0009 mM/min, FeUiO-1: 0.0053 mM/min). The degradation efficiency of BPA was increased by 100.8 times (UiO-66: 0.0012 min-1, FeUiO-1: 0.121 min-1), and the removal rate of TOC also reached 69.55%. The removal rate of BPA was maintained at more than 85% through 5 cycles. The reaction system was able to maintain a removal rate more than 97% at pH:3-9. In the presence of anions, such as Cl-, SO42-, NO32- (10 mM), the degradation rates of BPA were still above 94%. The catalytic efficiency was 2.02 times higher under natural light than relative to dark conditions. It was demonstrated by EPR and inhibition experiments that the main active species in the reaction were hydroxyl radicals and vacancies. The HOMO energy level and LUMO energy level of the intermediates were analyzed, and the possible degradation pathways of the active species were speculated. Evaluation of the biological toxicity of intermediates demonstrated that the system can effectively detoxify BPA. This investigation provides a reference method to enhance the efficiency of the photo-Fenton reaction of MOFs.

A Heating-Assisted Direct Ink Writing Method for Preparation of PDMS Cellular Structure with High Manufacturing Fidelity.

In response to the fact that most of the current research on silicone 3D printing suffers from structure collapse and dimensional mismatch, this paper proposes a heating-assisted direct writing printing method for commercial silicone rubber materials for preparing silicone foam with enhanced fidelity. In the experimental processes, the effects of substrate temperature, printing pressure, and printing speed on the filament width were investigated using a controlled variable method. The results showed the following: (1) the diameter of silicone rubber filaments was positively correlated with the printing pressure and substrate temperature, but negatively correlated with the printing speed; (2) the filament collapse of the large filament spaced foams was significantly improved by the addition of the thermal field, which, in turn, improved the mechanical properties and manufacturing stability of the silicon foams. The heating-assisted direct writing process in this paper can facilitate the development of the field of microelectronics and the direct printing of biomaterials.

Tailored d-Band Facilitating in Fe Gradient Doping CuO Boosts Peroxymonosulfate Activation for High Efficiency Generation and Release of Singlet Oxygen.

In the field of heterogeneous catalysis, limitations of the surface reaction process inevitably make improving the catalytic efficiency to remove pollutants in water a major challenge. Here, we report a unique structure of Fe surface-gradient-doped CuO that improves the overall catalytic processes of adsorption, electron transfer, and desorption. Interestingly, gradient doping leads to an imbalanced charge distribution in the crystal structure, thereby promoting the adsorption and electron transport efficiency of peroxymonosulfate (PMS). The orbital hybridization of Fe also improves the electronic activity. More importantly, the occupied d-orbital distribution is closer to the lower energy level, which improves the desorption of the reaction intermediate (1O2). As a result, the production and desorption of 1O2 have been improved, resulting in excellent BPA degradation ability (kinetic rate increased by 67.3 times). Two-dimensional infrared correlation spectroscopy is used to better understand the doping process and catalytic mechanism of Fe-CuO. Fe-O changes before Cu-O and is more active. The Fe-required active sites, active species intensity, and kinetic reaction rates show a good correlation. This research provides a scientific basis for expanding the purification of toxic organic pollutants in complex water environments by heterogeneous catalytic oxidation.

Autophagy inhibition facilitates wound closure partially dependent on the YAP/IL-33 signaling in a mouse model of skin wound healing.

Autophagy is a self-phagocytic and highly evolutionarily conserved intracellular lysosomal catabolic system, which plays a vital role in a variety of trauma models, including skin wound healing (SWH). However, the roles and potential mechanisms of autophagy in SWH are still controversial. We firstly investigated the role of autophagy in SWH-induced wound closure rate, inflammatory response, and histopathology, utilizing an inhibitor of autophagy 3-methyladenine (3-MA) and its agonist rapamycin (RAP). As expected, we found 3-MA treatment remarkably increased the wound closure rate, combated inflammation response, and mitigated histopathological changes, while RAP delivery aggravated SWH-induced pathological damage. To further exploit the underlying mechanism of autophagy regulating inflammation, the specific inhibitors of yes-associated protein (YAP), Verteporfin, and Anti-IL-33 were applied. Herein, treating with 3-MA markedly suppressed the expression of tumor necrosis factor-α (TNF-α), IL-1ß, and IL-6, promoted that of IL-10, IL-33, and ST2, while RAP administration reverted SWH-induced the up-regulation of these inflammatory cytokines mentioned above. Importantly, Verteporfin administration not only down-regulated the expression levels of YAP, TNF-α, and IL-6 but also up-regulated that of IL-33 and IL-10. Unexpectedly, 3-MA or RAP retreatment did not have any impact on the changes in IL-33 among these inflammatory indicators. Furthermore, elevated expression of IL-33 promoted wound closure and alleviated the pathological damage, whereas, its antagonist Anti-IL-33 treatment overtly reversed the above-mentioned effects of IL-33. Moreover, 3-MA in combination with anti-IL-33 treatment reversed the role of 3-MA alone in mitigated pathological changes, but they failed to revert the effect of anti-IL-33 alone on worsening pathological damage. In sum, emerging data support the novel contribution of the YAP/IL-33 pathway in autophagy inhibition against SWH-induced pathological damage, and highlight that the autophagy/YAP/IL-33 signal axis is expected to become a new therapeutic target for SWH.

Copper oxide/graphitic carbon nitride composite for bisphenol a degradation by boosted peroxymonosulfate activation: Mechanism of Cu-O covalency governs.

Surface structure can govern heterogeneous catalysis, resulting in its critical role in nonradical reactions. Here, we explored whether Cu-O covalency plays a critical role in controlling the inherent properties of copper oxide/graphitic carbon nitride (CuO-CN). Experiments and theoretical calculations show that, in contrast to the traditional concept of low-valent metal control activity, surface modification enlarges Cu-O covalency, and high-valent copper species at the surface easily bind peroxymonosulfate (PMS, (HSO5-)) anions. Therefore, optimized CuO-CN corresponds to a 14.8-fold higher kinetic reaction rate (0.10392 min-1) for PMS activation and pollutant degradation over those of unoptimized CuO-CN. Based on two-dimensional Fourier transform infrared correlation spectroscopy (2D-FT-IR-COS), Cu-O was determined to be the main active site. Cu-O is more active than other groups and acts before other groups. Benefiting from this electron transfer mechanism, CuO-CN shows good environmental tolerance (pH, anions, humic acid and actual water bodies such as tap water and groundwater). The established empirical kinetic model shows a strong linear correlation with the experimental kinetic reaction rate (> 0.94). CuO-CN/PMS can degrade organic pollutants efficiently for up to 30 days in a filter reactor. This work provides an understanding of the key role of the surface electronic structure in the nonradical activation of PMS and may provide support for improving the design of PMS catalysts.

Contamination of drinking water by neonicotinoid insecticides in China: Human exposure potential through drinking water consumption and percutaneous penetration.

Neonicotinoids (NEOs) are the most widely used pesticides and have posed a serious threat to human health. However, data on human exposure to NEOs are extremely scarce. To bridge this gap, human exposure potential of NEOs through drinking water consumption and percutaneous penetration was evaluated with the influences of 17 age groups, 4 seasons, 6 regions, and 2 genders. The results showed that drinking water in the present study had an upper middle level of NEO contamination. Anthropogenic activity and weather condition played important roles in the regional distribution of NEOs in tap water. For both children and adults, NEOs intake from drinking water exposure (NDE) and percutaneous exposure (NPE) in the south regions of China are significantly higher than those in the north regions, while the order of NDE and NPE by season is summer > spring = autumn > winter. Furthermore, human age and gender also have remarkable impacts on NDE and NPE. The age groups of children subjected to the highest NDE and NPE were 9 months - 2 years old and 9-12 years old, respectively. This study provides insights into the role of seasonal and regional influence, age and gender in the risk of drinking water and percutaneous exposure to NEOs.

Itgb3-integrin-deficient mice may not be a sufficient model for patients with Glanzmann thrombasthenia.

Itgb3­integrin­deficient (Itgb3­/­) mice have been reported as a Glanzmann thrombasthenia (GT) model and have been used for platelet research. However, it remains unclear whether this mouse model can fully simulate patients with GT or whether it has different characteristics from these patients. The present study aimed to answer this question. Itgb3­/­ mice were tested for platelet function, tail bleeding, whole­blood count, bone marrow hematopoiesis and organ enlargement. Itgb3­/­ platelets showed impaired functions, including fibrinogen binding, aggregation, adhesion or spreading. Itgb3­/­ mice demonstrated decreased platelet count and microcytic hypochromic anemia. Reduced iron staining of bone marrow and decreased plasma ferritin level confirmed the diagnosis of iron deficiency anemia. Evident splenomegaly was observed in Itgb3­/­ mice. Immunohistochemical analysis of spleen biopsy revealed normal expression of CD3 and CD19, but elevated expression of CD71, which suggested that the splenomegaly in Itgb3­/­ mice may be associated with extramedullary hematopoiesis. In conclusion, Itgb3­/­ mice exhibited some unique characteristics that differed from those of human patients with GT and thus cannot completely simulate patients with GT.

Study on the adsorption and desorption performance of magnetic resin for Congo red.

This study was to evaluate the adsorption capability of a magnetic resin (NDMP) to the removal of Congo red (CR) from aqueous solution. The adsorption kinetic and isotherm of NDMP were studied, as well as the desorption performance of NDMP. The results showed that the adsorption process of NDMP on CR was more suitable for Pseudo-second-order kinetic model. The whole adsorption process was affected by intraparticle diffusion and ion exchange. The adsorption isotherm of CR by NDMP was fitted better with Langmuir model. It showed that the adsorption of CR on NDMP resin was single layer adsorption. The maximum adsorption capacity (Qm) of CR at 308 K can reach 354.29 mg/g. In the desorption, 10% NaCl and NaOH eluents had better desorption rate for CR than other mass fraction. While NaCl(10%)-MeOH mixed eluent with volume ratio of 3:7 had the best regeneration performance. The desorption rate can reach 90% within 30 min. The adsorption performance of NDMP on CR didn't decrease after 13 times successive adsorption-desorption by NaCl(10%)-methanol eluent, indicating that NDMP can be efficiently regenerated. The excellent adsorption-desorption performance of NDMP on CR suggests that the magnetic resin has a great potential for treating CR dye wastewater.