[Effects of seed grading on population regularity degree and yield of summer maize]. / 种子分级对夏玉米群体整齐度和产量的影响.

Grading seeds based on grain size is an effective measure to improve population regularity degree and increase the yield of summer maize. Taking Denghai 605 as the experimental material, we set up a field experiment with treatments based on grain size: large seeds (L), medium-round seeds (MR), medium-flat seeds (MF), medium-round and medium-flat mixed seeds (MRF), and small seeds (S), with no-grading seeds as control (CK). We investigated seedling emergence rate, population regularity degree (including height, ear height and stem diameter), seedling sturdiness index, photosynthetic characteristics, dry matter accumulation and distribution characteristics, and yield. The results showed that the emergence rate followed an order of L>MR>MRF>MF>CK>S, with that of L treatment differed little from MR, MF and MRF treatments, but being significantly higher than S and CK treatments. Plant height and stem diameter population regularity degree of MRF treatment before seven-leaf stage was not different from those of L, MR, MF and S treatments, but significantly higher than those of CK. At the tasseling stage, all treatments had higher population regularity degree of plant height than other stages. Ear height population regularity degree of L, MR, MF, MRF, and S increased by 11.1%, 10.3%, 9.5%, 7.1%, and 6.4% compared with CK, respectively. The seedling sturdiness index of MRF treatment increased by 36.7% compared with S treatment, but was not significantly different from L treatment. The leaf area index of the L and MRF treatments was significantly higher than that of CK, and both had higher population photosynthetic properties. The population dry matter accumulation showed a pattern as L>MR>MRF>MF>CK>S. There was no significant difference among L, MR, and MRF treatments, but that in L being obviously higher than MF, CK, and S treatments. After seed grading, the number of harvested ears of the L and MRF treatments increased significantly, and the yield were shown as L>MR>MRF>MF>CK>S. There was no difference between the yield of MRF, MR and MF treatments. In conclusion, the performance of L treatment was improved but the number was small. Considering the grading cost and yield, the MRF treatment can save the seed amounts of sowing, realize mechanized sowing and precision sowing.

Structural identification of 4-benzyl-voglibose hydrochloride monohydrate using NMR and single-crystal X-ray diffraction methods.

The chemical structure studies on an important related substance of voglibose have been carried out using NMR spectroscopy and single crystal X-ray crystallography. For the structure identification study, hydrochloride monohydrate of this compound was isolated and purified. Its molecular structure was characterized and analyzed using 1D (1H NMR, 13C NMR, and DEPT) and 2D (1H,1H-COSY, HSQC, HMBC, TOCSY, and ROESY) NMR spectra. The signal crystal structure was detected by X-ray crystallography. All results indicated this related substance of voglibose was C17H27NO7·HCl·H2O, 5,6-dideoxy-4-benzyl-5-{[2-hydroxy-1-(hydroxymethyl)ethy]amino}-1-C-(hydroxymethyl)-D-epi-inositol hydrochloride monohydrate.

Effects of calcium and magnesium on acute and chronic neurotoxicity caused by oxaliplatin: A meta-analysis.

The primary toxicity of oxaliplatin is neurotoxicity. Calcium and magnesium (Ca/Mg) are reported to be beneficial in protecting against this adverse effect. However, the results obtained from clinical trials are not definitive. The aim of this study was to evaluate whether Ca/Mg alleviates the neurotoxicity of oxaliplatin by performing a meta-analysis of the literature involving available randomized controlled trials. Systematic searches for trials were undertaken from the Cochrane Library, MEDLINE, CENTRAL, Embase, CBMdisc and CNKI databases without language limitations. The primary outcome was severe chronic neurotoxicity and the secondary outcome was acute neurotoxicity. Four randomized double-blind trials met the search criteria. The odds ratio (OR) comparing Ca/Mg treatment with placebo was 0.44 (0.23-0.85, P=0.01) for severe chronic neurotoxicity of oxaliplatin (grade ≥2) and 0.41 (0.11-1.49, P=0.18) for acute neurotoxicity. In conclusion, Ca/Mg treatment does not reduce the incidence of acute neurotoxicity of oxaliplatin, but does reduce the incidence of severe chronic neurotoxicity (grade ≥2). No differences were observed in the outcomes of chemotherapy. Thus, Ca/Mg treatment is recommended for use as an adjunct with oxaliplatin.

[Therapeutic effect of indole-3-carbinol on pig serum-induced hepatic fibrosis in rats].

This study is to investigate the therapeutic effect and mechanism of indole-3-carbinol (I3C) on pig serum-induced liver fibrosis of rats. The liver fibrotic model of rats was induced by pig serum. After models were successfully established, rats in the treatment groups were administered with I3C through intraperitoneal injection or curcumin by intragastric administration, daily for 17 days. Hepatic hydroxyproline (Hyp) content was measured. The liver histology and immunohistochemistry with a-smooth muscle actin (alpha-SMA) were assayed. Hepatic stellate cells line, HSC-T6 was incubated with different concentrations of I3C (25, 50, and 100 micromol x L(-1)) for 24 h. The effect of I3C on cell apoptosis was identified by FITC-Annexin V/PI double labeled assay. And the mRNA expressions of Bax and Bcl-2 were measured by real time RT-PCR. The results showed that hepatic content of Hyp decreased by I3C treatment, as compared with the fibrotic model control. Histopathological changes, such as steatosis, necrosis, deposition of collagenous fiber reduced remarkably and the expression of alpha-SMA was significantly down-regulated in the I3C-treated groups (P < 0.01). Apoptosis analysis showed that I3C significantly increased HSC-T6 apoptosis rate and the expressional ratio of Bax to Bcl-2. The results indicated that I3C could effectively cure pig serum-induced liver fibrosis in vivo by inducing HSC apoptosis and promoting ECM degradation.

Indole-3-carbinol enhances the resolution of rat liver fibrosis and stimulates hepatic stellate cell apoptosis by blocking the inhibitor of κB kinase α/inhibitor of κB-α/nuclear factor-κB pathway.

Hepatic stellate cells (HSC) play a pivotal role in liver fibrosis, and the clearance of activated HSC by apoptosis is associated with the resolution of liver fibrosis. The development of strategies that promote this process in a selective way is therefore important. We evaluated the effects of indole-3-carbinol (I3C), a nutritional component derived from vegetables from the Brassica family, on liver fibrosis and HSC apoptosis. The in vivo therapeutic effects of I3C were monitored in three rat models of liver fibrosis induced by porcine serum, bile duct ligation, or multiple hepatotoxic factors, and its proapoptotic effect and molecular mechanism were studied in vitro in HSC-T6, a rat HSC line. The results showed that I3C treatment significantly reduced the number of activated HSC in the livers of rats with liver fibrosis. In histopathology, I3C reduced hepatocyte degeneration and necrosis, accelerated collagen degradation, and promoted the reversal of liver fibrosis. I3C prescribed to HSC-T6 resulted in morphologic alterations typical of apoptosis and DNA cleavage to a nucleosomal ladder. Moreover, I3C significantly increased the HSC-T6 apoptosis rate and the expression ratio of Bax to Bcl-2. High-throughput protein array analysis indicated that the tumor necrosis factor-α/nuclear factor-κB (NF-κB) signal pathway participated in I3C-induced HSC-T6 apoptosis. Western blot and electrophoretic mobility-shift assay confirmed that I3C inhibited the phosphorylation of inhibitor of κB kinase α and inhibitor of κB-α and NF-κB DNA binding activity. In conclusion, I3C could promote the reverse process of liver fibrosis in vivo and induce apoptosis of activated HSC in vitro, which indicates the use of I3C as a potential therapeutic agent in liver fibrosis treatment.

[Synthesis and anti-inflammatory analgesic activities of phenylfuroxan-coupled diclofenac].

AIM: To search for new derivatives of diclofenac (DC) having higher potency than the parent drug and lacking its undesirable effects. METHODS: Coupling DC with NO donor 3-hydroxymethyl-4-phenylfuroxan and its isomer through esterification, evaluating anti-inflammatory and analgesic activities, observing side effects in the rat gastrointestinal (GI) tract and assessing NO releasing ability both in vitro and in vivo. RESULTS: Fifteen new compounds including nine target ones (II1-9) were synthesized, and their structures were determined by IR, 1HNMR, MS and elemental analysis. Compounds II3 and II9 showed anti-inflammatory activity comparable to DC. Compound II2 showed stronger anti-inflammatory and analgesic activities and less GI side effect than DC, and released NO in vivo. CONCLUSION: Compound II2 is worthy to be intensively studied.