Spatial-temporal pattern and urban-rural gradient of comprehensive ecological security in urban agglomeration in South China from 2000 to 2020.

The ecological security (ES) of urban agglomeration and surrounding environmental system is related to the sustainable development of cities, which is a hot spot that we must pay attention to. In this study, four subsystems composed of natural base, landscape structure, ecosystem stability, and anthropogenic interference were used to evaluate the comprehensive ecological security (CES) of Guangdong-Hong Kong-Macao Greater Bay Area (GHMGBA) in 2000, 2010 and 2020. The results show that CES of the region was generally well. The central urban region of GHMGBA was unsafety with an area proportion of about 24.5%, the periphery was safety with an area proportion of about 43.5%, and the others are transitional zone. From 2000 to 2020, the CES change first slightly decreases and then relatively stable, and the transfer of different safety levels mainly occurs in the transitional zone. In 2010-2020 the transfer of different levels of CES is more frequent than in 2000-2010, indicating that the spatial-temporal pattern of CES fluctuated sharply during 2010-2020. The urban-rural gradient showed that with the increase of distance, CES fluctuations increase, but decreases at about 20-40km, 60-80km and 120-140km away from the city center, which may be sub-urban regions. The overall CES change range gradually decreases with increasing distance from urban centers. This study helps to understand the temporal and spatial distribution of ecological environment and urban-rural gradient in typical urban regions, and provides a reference for the collaborative planning of urban agglomeration.

The Role of Sfp1 in Candida albicans Cell Wall Maintenance.

The cell wall is the first interface for Candida albicans interaction with the surrounding environment and the host cells. Therefore, maintenance of cell wall integrity (CWI) is crucial for C. albicans survival and host-pathogen interaction. In response to environmental stresses, C. albicans undergoes cell wall remodeling controlled by multiple signaling pathways and transcription regulators. Here, we explored the role of the transcription factor Sfp1 in CWI. A deletion of the SFP1 gene not only caused changes in cell wall properties, cell wall composition and structure but also modulated expression of cell wall biosynthesis and remodeling genes. In addition, Cas5 is a known transcription regulator for C. albicans CWI and cell wall stress response. Interestingly, our results indicated that Sfp1 negatively controls the CAS5 gene expression by binding to its promoter element. Together, this study provides new insights into the regulation of C. albicans CWI and stress response.

Injection of Matrix Metalloproteinase-9 Leads to Ventricular Remodeling.

Objective: Previous studies have found that some ventricular remodeling is accompanied by increased matrix metalloproteinase-9 (MMP-9) in vivo, and MMP-9 inhibitors can reduce ventricular remodeling. However, there is still no direct evidence that MMP-9 causes ventricular remodeling. In this study, MMP-9 was injected into rats to observe whether MMP-9 caused ventricular remodeling, thereby providing direct evidence of MMP-9-induced ventricular remodeling. Methods: Forty-eight eight-week-old male Wistar rats were randomly divided, by weight, into control, low-, medium-, and high-dose MMP-9 groups and were administered normal saline or recombinant rat MMP-9 0.7, 1.4, or 2.1 ng/g, respectively, via intraperitoneal injection, twice per week. On the 28th day, six rats were randomly selected from each group (Stage I). The remaining rats continued receiving injections until the 56th day (Stage II). Echocardiography was performed to observe cardiac structure and function, and the left ventricular mass index (LVWI) was calculated. Myocardial pathological changes and the collagen volume fraction (CVF) were observed by HE and VG staining in myocardial tissue. MMP-9 levels in serum were tested using ELISA. Myocardial MMP-9 levels were measured using Western blots, and the myocardial expression levels of MMP-9 mRNA were assessed using RT-PCR. Results: During Stage I, serum MMP-9 and myocardial MMP-9 mRNA levels are increased; hypertrophic cardiomyocytes, disorderly arrangement of fibers, and endochylema dissolution are observed in the medium- and high-dose groups. The left ventricular weight index (LVWI) and myocardial MMP-9 increased, and the collagen volume fraction (CVF) reduced in the high-dose group. In Stage II, the left ventricular end-diastolic volume (LVEDV) and diameter (LVIDd) are higher, and CVF decreased in the medium- and high-dose groups. Myocardial pathological lesions intensified. Serum MMP-9 in the model groups and myocardial MMP-9 in the medium- and high-dose groups are increased. Conclusions: Injection of MMP-9 can lead to ventricular remodeling.

Spatial-temporal variations and pollution risks of mercury in water and sediments of urban lakes in Guangzhou City, South China.

This study aims to investigate the spatial and temporal characteristics, pollution degrees, and potential ecological risks of mercury (Hg) in urban lake waters and sediments in Guangzhou, where is a typical area of Hg emission and population-economic-industrial concentration in South China. In different districts of this city, the water from 15 lakes were collected continuously from June 2020 to May 2021, and the sediments from 9 lakes were collected in 2015 and 2021. The seasonal changes of Hg concentration (Hg-C) in the water were found to be high in winter and low in summer. The spatial distribution of Hg-C in sediments showed that it was high in urban central areas and low in suburbs. The Nemero index and geological accumulation index showed that there were uncontaminated of Hg in the collected lake water, and above moderately contaminated in the lake sediments in urban center, respectively. The Hg pollution potential ecological risk index showed that there was low risk in the collected water, high and extremely high risk in the lake sediments in urban center, respectively. The principal component analysis (PCA) and correlation analysis (CA) of Hg and meteorological factors showed that precipitation, temperature, and vapor pressure had negative effects on the seasonal changes of Hg-C in water, and air pressure and wind direction had positive effects. The PCA and CA of Hg and other geochemical elements showed that anthropogenic emissions may be the main sources of Hg in sediments, which was also supported by the data of population density, road density, and motor vehicles per 1000 people. This study provided a reference for urban lake pollution treatment, resident health, and ecological environment protection.

Dynamic physical examination indicators of cardiovascular health: A single-center study in Shanghai, China.

Dynamic physical examination data can provide both cross-sectional and time-series characteristics of cardiovascular health. However, most physical examination databases containing health and disease information have not been fully utilized in China. Hence, this study aimed to analyze dynamic physical examination indicators for cardiovascular health to provide evidence for precise prevention and control of cardiovascular diseases in the primary prevention domain among healthy population with different demographic characteristics in Shanghai. Three-year continuous data were collected from the physical examination center of a hospital in Shanghai from 2018 to 2020, which included a total of 14,044 participants with an average age of 46.51±15.57 years. The cardiovascular status of overall healthy individuals may have a decreasing trend, which is manifested as a significant year-on-year decrease in high-density lipoprotein cholesterol; a significant year-on-year increase in total cholesterol, low-density lipoprotein cholesterol, and blood glucose levels; and a possible increasing trend of diastolic blood pressure, body mass index, and triglycerides. Healthy population with different sex and age groups have various sensitives to cardiovascular physical examination indicators. To conduct more accurate cardiovascular health management and health promotion for key populations in primary prevention, focusing on the dynamic trends of blood pressure, blood lipids, blood glucose, and body mass index in men and changes in total cholesterol in women over time is especially important. The age group of 50-69 years is key for better prevention and control of cardiovascular health.

A Comparison of Numerical and Actual Measurements of Large-Scale Rib-Structured Pallet Flatness Using Recycled Polypropylene in Injection Molding.

Many challenges are associated with the injection molding process for forming a rib-structured pallet (1100 mm × 1100 mm × 140 mm, length × width × height) because greater flowing resistance through the rib channels within the pallet can induce insufficient filling. Essentially, multi-gate filling involves a sequential valve gate system, which helps to spread the filling front with fewer weld lines. Based on the presetting of the sequential scheme of the valve gates, actual measurements of pallet flatness using the ATOS scan system were compared to numerical warpage measurements of a pallet derived by Moldex3D 2020. In this study, we propose a sequential scheme by actuating the valve gates to open once the flow front spreads towards them; then, actual warpage measurements of a pallet are compared with numerical measurements. The results show that the warpage of the top surface of the pallet is 5.144 mm in actual measurements and 5.729 mm in simulation. The results all indicated small warpage with respect to the pallet size. The simulation and actual measurements of flatness are in excellent agreement; the difference in top flatness between the simulated and actual pallet is 0.59 mm, while the bottom flatness difference is 0.035 mm. By adjusting the cooling water temperature, increasing the mold temperature, and decreasing the material temperature, overall flatness and warpage displacement can be reduced.

Investigation of the Warpage of a High-Density Polyethylene Pallet by Plastic Injection Compression Molding: Part I-Numerical Approach.

Many challenges are associated with the injection compression molding process for producing a half-pallet (1320 mm × 1110 mm × 75 mm, length × width × height), which is butt-welded to another one for enhancing its strength. This pooled high-density polyethylene (HDPE) pallet is able to endure the impacts of a heavy load and a low ambient temperature. Reducing the warpage of a half-pallet is, therefore, essential for reducing the residual internal stress within the welded portions. An advanced Moldex3D package helps to detail the temperature distribution and warpage of a half-pallet. The pre-setting molding parameters from a mass-production factory produce half-pallets with worse flatness. In this investigation on using appropriate cooling water temperatures within the core and cavity plates of the mold, the numerical results show that the warpage of the top surface of the half-pallet was 11.549 mm, low warpage with respect to this large-scale pallet. Furthermore, the compression speed of 50-60 mm/s may have produced a low flatness of the half-pallet in this study.

Candida albicans Sfp1 Is Involved in the Cell Wall and Endoplasmic Reticulum Stress Responses Induced by Human Antimicrobial Peptide LL-37.

Candida albicans is a commensal fungus of humans but can cause infections, particularly in immunocompromised individuals, ranging from superficial to life-threatening systemic infections. The cell wall is the outermost layer of C. albicans that interacts with the host environment. Moreover, antimicrobial peptides (AMPs) are important components in innate immunity and play crucial roles in host defense. Our previous studies showed that the human AMP LL-37 binds to the cell wall of C. albicans, alters the cell wall integrity (CWI) and affects cell adhesion of this pathogen. In this study, we aimed to further investigate the molecular mechanisms underlying the C. albicans response to LL-37. We found that LL-37 causes cell wall stress, activates unfolded protein response (UPR) signaling related to the endoplasmic reticulum (ER), induces ER-derived reactive oxygen species and affects protein secretion. Interestingly, the deletion of the SFP1 gene encoding a transcription factor reduced C. albicans susceptibility to LL-37, which is cell wall-associated. Moreover, in the presence of LL-37, deletion of SFP1 attenuated the UPR pathway, upregulated oxidative stress responsive (OSR) genes and affected bovine serum albumin (BSA) degradation by secreted proteases. Therefore, these findings suggested that Sfp1 positively regulates cell wall integrity and ER homeostasis upon treatment with LL-37 and shed light on pathogen-host interactions.

Ox-LDL Aggravates the Oxidative Stress and Inflammatory Responses of THP-1 Macrophages by Reducing the Inhibition Effect of miR-491-5p on MMP-9.

Background: Oxidized low-density lipoprotein (ox-LDL) can induce oxidative stress and inflammatory responses in macrophages to facilitate the genesis and development of atherosclerosis. However, the intermediate links remain unclear. MiR-491-5P can inhibit matrix metalloproteinase 9 (MMP-9); however, it remains unclear whether ox-LDL enhances MMP-9 expression and aggravates the oxidative stress and inflammatory responses under the mediating effect of miR-491-5P. Method: THP-1 macrophages were divided into 10 groups: blank (control), model (ox-LDL), miR-491-5P high-expression (miR-491-5P mimic), miR-491-5P control (mimic-NC), MMP-9 high-expression (MMP-9-plasmid), MMP-9 control (plasmid-NC), miR-491-5P+plasmid-NC, miR-491-5P+ MMP-9-plasmid, MMP-9 gene silencing (MMP-9-siRNA), and gene silencing control (siRNA-NC). The cells were transfected for 48 h and then treated with 50 µg/mL of ox-LDL for 24 h. MMP-9 mRNA and miR-491-5P expression levels in the cells were detected using reverse transcription-quantitative polymerase chain reaction, and the MMP-9 levels were detected with western blotting. The levels of oxidative stress factors (malondialdehyde [MDA]), reactive oxygen species (ROS), and antioxidant factors (superoxide dismutase [SOD]), and the expression levels of inflammatory factors (tumor necrosis factor [TNF-α] and interleukin-1ß and-6 [IL-1ß and IL-6]) in the supernatant were detected with enzyme-linked immunosorbent assay. Results: MDA, ROS, TNF-α, IL-1ß, IL-6, and MMP-9 levels were increased, SOD activity was reduced, and miR-491-5P expression was downregulated in the ox-LDL group compared to the control group. In the miR-491-5P mimic group, the MDA, ROS, TNF-α, IL-1ß, IL-6, MMP-9 mRNA and protein levels were downregulated, and SOD activity was enhanced compared to the ox-LDL group. MMP-9-plasmid elevated the MDA, ROS, TNF-α, IL-1ß, IL-6, MMP-9 mRNA and protein levels, and downregulated SOD activity and miR-491-5P expression. Following transfection with MMP-9-siRNA, the MMP-9-plasmid outcomes were nullified, and the resulting trends were similar to the miR-491-5p simulation group. Oxidative stress and inflammatory responses were higher in the miR-491-5P mimic+MMP-9-plasmid co-transfection group than in the miR-491-5P mimic group. Conclusion: Ox-LDL aggravates the oxidative stress and inflammatory responses of THP-1 macrophages by reducing the inhibition effect of miR-491-5p on MMP-9.