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Klin Lab Diagn ; 63(3): 183-186, 2018.
Artículo en Ruso | MEDLINE | ID: mdl-30673200

RESUMEN

Alternative reading frame encoding a single protein known as protein F or core + 1 / ARFP is located in the core region of the hepatitis C virus (HCV) genome. The presence of antibodies to the F protein of HCV in the serum of patients with chronic hepatitis C indicates the expression of this protein in vivo. In this study, to determine antibodies to the F protein of HCV in serum samples the methodology of the enzyme immunoassay (ELISA) was developed using the synthetic peptide F10 corresponding to the antigenic determinant of the F protein of the HCV subtype 1b. The immunogenicity and immunochemical specificity of synthetic F10 peptide has been demonstrated in laboratory animals (mice).


Asunto(s)
Hepatitis C Crónica , Animales , Anticuerpos , Ensayo de Inmunoadsorción Enzimática , Hepacivirus , Anticuerpos contra la Hepatitis C , Humanos , Ratones , Péptidos , Proteínas del Núcleo Viral
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