The influencing factors of individual interest in physical education based on decision tree model: A cross-sectional study.

To identify the key influencing factors and analyze the internal relationship among the factors of individual interest in PE, we conducted a cross-sectional survey of a large sample of Chinese young students based on the decision tree model. A total of 3,640 young students (M age = 14.16; 7-18 years; SD = 2.66, 47% boys) were investigated by using six questionnaires, including individual interest in physical PE, self-efficacy, achievement goals, expectancy value in PE, PE knowledge and skills and PE learning environment. Results showed there were a total of seven variables entered into the decision tree model, which was 3 layers high, including 38 nodes. The root node was expectancy value which was divided by sports knowledge and skills and self-efficacy. The third layer included mastery-approach goal, family sports environment, performance-avoidance goal and gender. The results depict that expectancy value of PE was the most important influencing factors of adolescent students' individual interest in PE in this study, and the other important factors were sports knowledge and skills, self-efficacy, mastery-approach goal, family sports environment, performance-avoidance goal, and gender, respectively. The implications for PE are: (1) Improve the status of the PE curriculum and enhance students' recognition of the value of PE; (2) Strengthen the teaching of knowledge and skills to avoid low-level repetitive teaching; (3) Enhance success experience and foster sports self-efficacy; and (4) Establish reasonable sports goals to foster individual interest in sports learning.

Identification of Crucial Genes and Key Functions in Type 2 Diabetic Hearts by Bioinformatic Analysis.

Type 2 diabetes (T2D) patients with SARS-CoV-2 infection hospitalized develop an acute cardiovascular syndrome. It is urgent to elucidate underlying mechanisms associated with the acute cardiac injury in T2D hearts. We performed bioinformatic analysis on the expression profiles of public datasets to identify the pathogenic and prognostic genes in T2D hearts. Cardiac RNA-sequencing datasets from db/db or BKS mice (GSE161931) were updated to NCBI-Gene Expression Omnibus (NCBI-GEO), and used for the transcriptomics analyses with public datasets from NCBI-GEO of autopsy heart specimens with COVID-19 (5/6 with T2D, GSE150316), or dead healthy persons (GSE133054). Differentially expressed genes (DEGs) and overlapping homologous DEGs among the three datasets were identified using DESeq2. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses were conducted for event enrichment through clusterProfile. The protein-protein interaction (PPI) network of DEGs was established and visualized by Cytoscape. The transcriptions and functions of crucial genes were further validated in db/db hearts. In total, 542 up-regulated and 485 down-regulated DEGs in mice, and 811 up-regulated and 1399 down-regulated DEGs in human were identified, respectively. There were 74 overlapping homologous DEGs among all datasets. Mitochondria inner membrane and serine-type endopeptidase activity were further identified as the top-10 GO events for overlapping DEGs. Cardiac CAPNS1 (calpain small subunit 1) was the unique crucial gene shared by both enriched events. Its transcriptional level significantly increased in T2D mice, but surprisingly decreased in T2D patients with SARS-CoV-2 infection. PPI network was constructed with 30 interactions in overlapping DEGs, including CAPNS1. The substrates Junctophilin2 (Jp2), Tnni3, and Mybpc3 in cardiac calpain/CAPNS1 pathway showed less transcriptional change, although Capns1 increased in transcription in db/db mice. Instead, cytoplasmic JP2 significantly reduced and its hydrolyzed product JP2NT exhibited nuclear translocation in myocardium. This study suggests CAPNS1 is a crucial gene in T2D hearts. Its transcriptional upregulation leads to calpain/CAPNS1-associated JP2 hydrolysis and JP2NT nuclear translocation. Therefore, attenuated cardiac CAPNS1 transcription in T2D patients with SARS-CoV-2 infection highlights a novel target in adverse prognostics and comprehensive therapy. CAPNS1 can also be explored for the molecular signaling involving the onset, progression and prognostic in T2D patients with SARS-CoV-2 infection.

Evaluation of the correlation between infrared thermal imaging-magnetic resonance imaging-pathology of microwave ablation of lesions in rabbit lung tumors.

PURPOSE: This study aimed to evaluate the correlation between infrared thermal imaging-magnetic resonance imaging (MRI)-pathology of microwave ablation (MWA) of lesions in rabbit lung tumors. MATERIALS AND METHODS: MR-guided MWA was performed in nine VX2 tumor-bearing rabbits. Infrared thermal imaging, postoperative MRI, and pathological presentation were obtained and analyzed. The differences between the infrared thermal imaging-MRI-pathology of MWA were compared. RESULTS: The center of the ablated lesion exhibited a high signal on T1-Vibe, and an isointense envelope was observed; the center of the ablated lesion exhibited a low signal on fat-suppressed turbo spin-echo T2-weighted imaging (TSE-T2WI-FS) and bands of high signal surrounding it compared with before MWA. No statistically significant difference existed between the maximum diameter of the central low-signal area of the ablation zone on TSE-T2WI-FS after MWA, the high-signal area of the ablation zone on T1-Vibe after MWA, and the maximum diameter of the pathological coagulation necrosis area, as well as between the maximum diameter of the isointense signal area peripheral to the ablation zone on T1-Vibe after MWA, the high-signal area peripheral to the ablation zone on TSE-T2WI-FS, the maximum diameter at the 41°C isothermal zone on infrared thermal imaging, and the maximum diameter of the pathological thermal injury zone. CONCLUSIONS: MWA of malignant lung tumors had specific MRI characteristics that were comparable with postoperative pathology. Infrared thermal imaging combined with MRI can be used to evaluate the extent of thermal damage to lung VX2 tumors.

[The role of matrix metalloproteinases-9 and tissue inhibitor of metalloproteinaes-1 in chronic obstructive pulmonary disease].

OBJECTIVE: To evaluate the role of matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metalloproteinases-1 (TIMP-1) in the pathogenesis of chronic obstructive pulmonary disease (COPD). METHODS: Five milliliters of peripheral venous blood were extracted form 36 patients with COPD, subdivided into stable group (CS group, n = 12), exacerbation group (CE group, n = 12), and exacerbation group with glucocorticosteroids treatment (CE + G group, n = 12), and 12 normal controls. The peripheral blood mononuclear cells (PBMCs) were isolated and the mRNA expressions of MMP-9 and TIMP-1 therein in each group were measured by RT-PCR quantitative analysis. The MMP-2 and MMP-9 levels in the plasma were studied by gelatin zymography. The relationship between MMP and pulmonary function was evaluated. RESULTS: (1) The MMP-9 mRNA expression and MMP-9: TIMP-1 ratios in the samples obtained from patients with COPD were higher in comparison with those from the control group (all P < 0.05). (2) The MMP-9 levels in plasma were higher in the COPD groups than in the control group (P < 0.05). (3) In the COPD groups, the MMP-9 mRNA expression, MMP-9: TIMP-1 ratio, and MMP-9 level in plasma were negatively correlated with FEV(1.0)% of prediction (r = -0.789, P < 0.05; r = -0.53, P < 0.05; and r = -0.652, P < 0.05), and positively correlated with RV/TLC% (r = 0.375, P < 0.05; r = 0.44, P < 0.05; and r = 0.529, P < 0.05). CONCLUSION: MMP-9 and the imbalanced ratio of MMP-9: TIMP-1 may not only play a role in the pathogenesis of COPD, but also relate to FEV(1.0)% of prediction and RV/TLC%.