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1.
Nature ; 613(7945): 712-720, 2023 01.
Artículo en Inglés | MEDLINE | ID: mdl-36653451

RESUMEN

Ribosomes are produced in large quantities during oogenesis and are stored in the egg. However, the egg and early embryo are translationally repressed1-4. Here, using mass spectrometry and cryo-electron microscopy analyses of ribosomes isolated from zebrafish (Danio rerio) and Xenopus laevis eggs and embryos, we provide molecular evidence that ribosomes transition from a dormant state to an active state during the first hours of embryogenesis. Dormant ribosomes are associated with four conserved factors that form two modules, consisting of Habp4-eEF2 and death associated protein 1b (Dap1b) or Dap in complex with eIF5a. Both modules occupy functionally important sites and act together to stabilize ribosomes and repress translation. Dap1b (also known as Dapl1 in mammals) is a newly discovered translational inhibitor that stably inserts into the polypeptide exit tunnel. Addition of recombinant zebrafish Dap1b protein is sufficient to block translation and reconstitute the dormant egg ribosome state in a mammalian translation extract in vitro. Thus, a developmentally programmed, conserved ribosome state has a key role in ribosome storage and translational repression in the egg.


Asunto(s)
Secuencia Conservada , Evolución Molecular , Óvulo , Biosíntesis de Proteínas , Ribosomas , Proteínas de Xenopus , Proteínas de Pez Cebra , Animales , Microscopía por Crioelectrón/métodos , Péptidos/metabolismo , Ribosomas/metabolismo , Pez Cebra/embriología , Pez Cebra/metabolismo , Espectrometría de Masas , Xenopus laevis/embriología , Óvulo/metabolismo , Estructuras Embrionarias , Desarrollo Embrionario , Femenino , Factor 5A Eucariótico de Iniciación de Traducción
2.
Dev Growth Differ ; 64(5): 243-253, 2022 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-35581155

RESUMEN

Investigating cell lineage requires genetic tools that label cells in a temporal and tissue-specific manner. The bacteriophage-derived Cre-ERT2 /loxP system has been developed as a genetic tool for lineage tracing in many organisms. We recently reported a stable transgenic Xenopus line with a Cre-ERT2 /loxP system driven by the mouse Prrx1 (mPrrx1) enhancer to trace limb fibroblasts during the regeneration process (Prrx1:CreER line). Here we describe the detailed technological development and characterization of such line. Transgenic lines carrying a CAG promoter-driven Cre-ERT2 /loxP system showed conditional labeling of muscle, epidermal, and interstitial cells in both the tadpole tail and the froglet leg upon 4-hydroxytamoxifen (4OHT) treatment. We further improved the labeling efficiency in the Prrx1:CreER lines from 12.0% to 32.9% using the optimized 4OHT treatment regime. Careful histological examination showed that Prrx1:CreER lines also sparsely labeled cells in the brain, spinal cord, head dermis, and fibroblasts in the tail. This work provides the first demonstration of conditional, tissue-specific cell labeling with the Cre-ERT2 /loxP system in stable transgenic Xenopus lines.


Asunto(s)
Integrasas , Animales , Animales Modificados Genéticamente , Integrasas/genética , Integrasas/metabolismo , Ratones , Ratones Transgénicos , Regiones Promotoras Genéticas , Xenopus laevis/genética , Xenopus laevis/metabolismo
3.
Dev Cell ; 56(10): 1541-1551.e6, 2021 05 17.
Artículo en Inglés | MEDLINE | ID: mdl-34004152

RESUMEN

Limb regeneration, while observed lifelong in salamanders, is restricted in post-metamorphic Xenopus laevis frogs. Whether this loss is due to systemic factors or an intrinsic incapability of cells to form competent stem cells has been unclear. Here, we use genetic fate mapping to establish that connective tissue (CT) cells form the post-metamorphic frog blastema, as in the case of axolotls. Using heterochronic transplantation into the limb bud and single-cell transcriptomic profiling, we show that axolotl CT cells dedifferentiate and integrate to form lineages, including cartilage. In contrast, frog blastema CT cells do not fully re-express the limb bud progenitor program, even when transplanted into the limb bud. Correspondingly, transplanted cells contribute to extraskeletal CT, but not to the developing cartilage. Furthermore, using single-cell RNA-seq analysis we find that embryonic and adult frog cartilage differentiation programs are molecularly distinct. This work defines intrinsic restrictions in CT dedifferentiation as a limitation in adult regeneration.


Asunto(s)
Diferenciación Celular , Fibroblastos/citología , Regeneración/fisiología , Ambystoma mexicanum , Animales , Tipificación del Cuerpo , Cartílago/citología , Reprogramación Celular , Células del Tejido Conectivo/citología , Dermis/citología , Embrión no Mamífero/citología , Larva , Xenopus laevis/embriología
4.
J Neurogenet ; 33(2): 52-63, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30939963

RESUMEN

Several large or mid-scale collections of Drosophila enhancer traps have been recently created to allow for genetic swapping of GAL4 coding sequences to versatile transcription activators or suppressors such as LexA, QF, split-GAL4 (GAL4-AD and GAL4-DBD), GAL80 and QS. Yet a systematic analysis of the feasibility and reproducibility of these tools is lacking. Here we focused on InSITE GAL4 drivers that specifically label different subpopulations of olfactory neurons, particularly local interneurons (LNs), and genetically swapped the GAL4 domain for LexA, GAL80 or QF at the same locus. We found that the major utility-limiting factor for these genetic swaps is that many do not fully reproduce the original GAL4 expression patterns. Different donors exhibit distinct efficacies for reproducing original GAL4 expression patterns. The successfully swapped lines reported here will serve as valuable reagents and expand the genetic toolkits of Drosophila olfactory circuit research.


Asunto(s)
Animales Modificados Genéticamente/genética , Proteínas de Drosophila/genética , Técnicas Genéticas , Factores de Transcripción/genética , Animales , Drosophila , Femenino , Masculino
5.
Nat Commun ; 9(1): 4729, 2018 11 06.
Artículo en Inglés | MEDLINE | ID: mdl-30401872

RESUMEN

The original version of this Article contained errors in Figs. 4 and 6. In Fig. 4, panel a, text labels UAS-FLP and LexAop2>stop>myr::smGdP-HA were shifted upwards during typesetting of the figure, and in Fig. 6, panel h, the number 15 was incorrectly placed on the heat map scale. These have now been corrected in both the PDF and HTML versions of the Article.

6.
Nat Commun ; 9(1): 2232, 2018 06 08.
Artículo en Inglés | MEDLINE | ID: mdl-29884811

RESUMEN

Drosophila olfactory local interneurons (LNs) in the antennal lobe are highly diverse and variable. How and when distinct types of LNs emerge, differentiate, and integrate into the olfactory circuit is unknown. Through systematic developmental analyses, we found that LNs are recruited to the adult olfactory circuit in three groups. Group 1 LNs are residual larval LNs. Group 2 are adult-specific LNs that emerge before cognate sensory and projection neurons establish synaptic specificity, and Group 3 LNs emerge after synaptic specificity is established. Group 1 larval LNs are selectively reintegrated into the adult circuit through pruning and re-extension of processes to distinct regions of the antennal lobe, while others die during metamorphosis. Precise temporal control of this pruning and cell death shapes the global organization of the adult antennal lobe. Our findings provide a road map to understand how LNs develop and contribute to constructing the olfactory circuit.


Asunto(s)
Drosophila melanogaster/metabolismo , Interneuronas/metabolismo , Vías Olfatorias/metabolismo , Neuronas Receptoras Olfatorias/metabolismo , Animales , Animales Modificados Genéticamente , Antenas de Artrópodos/citología , Antenas de Artrópodos/crecimiento & desarrollo , Antenas de Artrópodos/metabolismo , Drosophila melanogaster/genética , Drosophila melanogaster/crecimiento & desarrollo , Interneuronas/clasificación , Larva/crecimiento & desarrollo , Larva/metabolismo , Microscopía Confocal , Modelos Neurológicos , Morfogénesis , Red Nerviosa/citología , Red Nerviosa/crecimiento & desarrollo , Red Nerviosa/metabolismo , Vías Olfatorias/citología , Vías Olfatorias/crecimiento & desarrollo , Neuronas Receptoras Olfatorias/clasificación , Transmisión Sináptica , Factores de Tiempo
7.
Int J Biol Sci ; 8(5): 761-77, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22701344

RESUMEN

The tristetraprolin (TTP) family comprises zinc finger-containing AU-rich element (ARE)-binding proteins consisting of three major members: TTP, ZFP36L1, and ZFP36L2. The present study generated specific antibodies against each TTP member to evaluate its expression during differentiation of 3T3-L1 preadipocytes. In contrast to the inducible expression of TTP, results indicated constitutive expression of ZFP36L1 and ZFP36L2 in 3T3-L1 preadipocytes and their phosphorylation in response to differentiation signals. Physical RNA pull-down and functional luciferase assays revealed that ZFP36L1 and ZFP36L2 bound to the 3' untranslated region (UTR) of MAPK phosphatase-1 (MKP-1) mRNA and downregulated Mkp-1 3'UTR-mediated luciferase activity. Mkp-1 is an immediate early gene for which the mRNA is transiently expressed in response to differentiation signals. The half-life of Mkp-1 mRNA was longer at 30 min of induction than at 1 h and 2 h of induction. Knockdown of TTP or ZFP36L2 increased the Mkp-1 mRNA half-life at 1 h of induction. Knockdown of ZFP36L1, but not ZFP36L2, increased Mkp-1 mRNA basal levels via mRNA stabilization and downregulated ERK activation. Differentiation induced phosphorylation of ZFP36L1 through ERK and AKT signals. Phosphorylated ZFP36L1 then interacted with 14-3-3, which might decrease its mRNA destabilizing activity. Inhibition of adipogenesis also occurred in ZFP36L1 and TTP knockdown cells. The findings indicate that the differential expression of TTP family members regulates immediate early gene expression and modulates adipogenesis.


Asunto(s)
Tristetraprolina/metabolismo , Proteínas 14-3-3/metabolismo , Células 3T3-L1 , Animales , Factor 1 de Respuesta al Butirato , Diferenciación Celular/genética , Diferenciación Celular/fisiología , Línea Celular , Fosfatasa 1 de Especificidad Dual/genética , Fosfatasa 1 de Especificidad Dual/metabolismo , Humanos , Immunoblotting , Ratones , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Fosforilación , Unión Proteica , Proteínas de Unión al ARN/genética , Proteínas de Unión al ARN/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Tristetraprolina/genética
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