Accurate and rapid mercury susceptibility detection in aquatic samples using fluorescent probe integrated rhodamine with pyridyl isothiocyanate.

Mercury, one of the various harmful metals, is particularly significant in affecting aquatic organisms, currently gaining more attentions and sparking discussions. In response to the limitations of traditional detections, fluorescent probes have emerged as a promising solution with some advantages, such as weaker background interference, shorter processing time, higher accuracy. Thus, a novel fluorescent probe, FS-Hg-1, has been developed for assessing mercury ion (Hg2+) concentrations in aquatic products. This probe displays specific recognition of mercury ions in fluorescence spectra. Notably, FS-Hg-1 exhibits a distinct color change to pink when combined with Hg2+ (with a 948-fold increase in absorption at 568 nm) and a substantial fluorescence change towards Hg2+ (361-fold increase, excitation at 562 nm, emission at 594 nm) in N, N-dimethylformamide. The probe boasts a detection limit of 0.14 µM and rapid reaction with Hg2+ within 10 s, showing an excellent linear correlation with [Hg2+] in the range of 0 to 10 µM. Through thorough analysis using FS-Hg-1, the results align with those from the standard method (P > 0.05), with spiked recovery rates ranging from 108.4% to 113.2%. With its precise recognition, low detection limit, and remarkable sensitivity, this fluorescent assay proves effective in mercury concentration determination in aquatic samples without interference. The potential of FS-Hg-1 is promising for speedy detection of residual Hg2+ and holds significance in ensuring food safety.

Practical NIR Assay Derived from Cyanine to Evaluate Intracellular H2S in Living Cell Imaging.

To monitor the biological function of H2S in real time, this investigation demonstrated the design and synthesis of a novel fluorescent probe integrated with cyanine and 2,4-dinitrophenol for the qualitative and quantitative detection of H2S. An NIR sensitive sensor (FS-HS-1) was provided with a straightforward process. Spectroscopy experiments elucidated that FS-HS-1 could selectively detect H2S in a PBS solution (containing 40% acetonitrile) with a 111-fold fluorescence enhancement at 715 nm (ex. 605 nm). The response towards NaHS occurred in less than 2 min, and the detection limit was confirmed to be as low as 4.47 ± 0.11 nmol/L. Furthermore, the probe is capable of monitoring changes in exogenous H2S concentrations within living cells with confocal and 2P imaging.

Scarring Skin: Mechanisms and Therapies.

Skin injury always results in fibrotic, non-functional scars in adults. Although multiple factors are well-known contributors to scar formation, the precise underlying mechanisms remain elusive. This review aims to elucidate the intricacies of the wound healing process, summarize the known factors driving skin cells in wounds toward a scarring fate, and particularly to discuss the impact of fibroblast heterogeneity on scar formation. To the end, we explore potential therapeutic interventions used in the treatment of scarring wounds.

Changes in gut microbiota and short-chain fatty acids are involved in the process of canine obesity after neutering.

Neutering is a significant risk factor for obesity in dogs. Changes in gut microbiota and its metabolites have been identified as a key player during obesity progression. However, the mechanisms that promote neuter-associated weight gain are not well understood. Therefore, in this study, sixteen clinically healthy Beagle dogs (6 male and 10 female, mean age = 8.22 ±â€…0.25 mo old) were neutered. Body weight (BW) and body condition score (BCS) were recorded at 1 d before neutering, 3, 6, 10, 16, and 21 mo after neutering. Dogs were grouped based on their BCS as ideal weight group (IW, n = 4, mean BW = 13.22 ±â€…1.30 kg, mean BCS = 5.00 ±â€…0.41) and obese group (OB, n = 12, mean BW = 18.57 ±â€…1.08 kg, mean BCS = 7.92 ±â€…0.82) at 21 mo after neutering. Serum lipid profile, glucose, and hormones and fecal microbiota and short-chain fatty acids (SCFAs) were measured. Our results showed that OB dogs had greater (P < 0.0001) BW (18.57 vs. 13.22 kg), BCS (7.92 vs. 5.00), and average daily gain (12.27 vs. 5.69 g/d) than IW dogs at 21 mo after neutering, and the obesity rate was up to 60%. In addition, significant increases (P < 0.05) in serum triglyceride (TG, 1.10 vs. 0.56 mmol/L) and high-density lipoprotein cholesterol (HDL-C, 6.96 vs. 5.40 mmol/L) levels and a significant decrease (P < 0.05) in serum adiponectin (APN, 54.06 vs. 58.39 µg/L) level were observed in OB dogs; serum total cholesterol (4.83 vs. 3.75 mmol/L) (P = 0.075) and leptin (LEP, 2.82 vs. 2.53 µg/L) (P = 0.065) levels tended to be greater in OB dogs; there was a trend towards a lower (P = 0.092) APN/LEP (19.32 vs. 21.81) in OB dogs. Results of fecal microbial alpha-diversity showed that Observed_species and Chao1 indices tended to be lower (P = 0.069) in OB dogs. The STAMP and LEfSe analyses revealed that OB dogs had a greater (P < 0.05 and LDA > 2) reduction in relative abundances of Bacteroides, Prevotella_9, and Megamonas than IW dogs. In addition, OB dogs also had greater (P < 0.05) reduction in fecal acetate, propionate, and butyrate concentrations than IW dogs. Moreover, clear negative correlations (|r| > 0.5 and P < 0.05) were found between SCFAs-producing bacteria and BW, TG, and HDL-C. The functional predictions of microbial communities based on PICRUSt2 analysis revealed that lipid metabolism and endocrine system were significantly disturbed in obese dogs after neutering. Thus, intervention with SCFAs-producing bacteria might represent a new target for the prevention or treatment of canine obesity after neutering. Moreover, weight control before neutering may also contribute to the prevention of canine obesity after neutering.

Neutering contributes to canine obesity risk. In this study, obesity rate of 60% at 21 mo after neutering was observed. Obese dogs had greater serum triglyceride, total cholesterol, high-density lipoprotein cholesterol, and leptin levels and lower adiponectin level than ideal weight dogs. In addition, fecal microbiota analysis found a decreasing microbial diversity in obese dogs, and decreasing SCFAs-producing bacteria Megamonas, Bacteroides, and Prevotella_9 in obese dogs resulted in lower production of fecal acetate, propionate, and butyrate. Importantly, strong negative correlations between SCFAs-producing bacteria and body weight, TG, and HDL-C revealed that SCFAs-producing bacteria are involved in the process of canine obesity after neutering. Thus, intervention with SCFAs-producing bacteria may be a target for the prevention or treatment of canine obesity after neutering. Moreover, weight control before neutering may also contribute to the prevention of canine obesity after neutering.

Wet-food diet promotes the recovery from surgery of castration and control of body weight in adult young cats.

Inappropriate dietary management may lead to delayed recovery from castration surgery and significant weight gain in cats after castration. Wet canned food often exhibits more advantageous characteristics than dry food (e.g., higher palatability and digestibility, and lower energy density). This study compared the effects of canned and dry food on surgical recovery and weight management in cats after castration. Eighteen healthy cats (weighed 4.33 ± 1.04 kg and aged 18-months old) were allocated to one of the two dietary treatments (N = 9/group), dry (CON) and canned food (CAN) balanced for sex and initial BW. Cats were fed ad libitum for 7 weeks, including one week before surgery (week 0) and 6 weeks after surgery (week 1-6). Daily dry matter intake (DMI), and weekly body weight (BW) and body condition score (BCS) was obtained. Feces were collected for measuring nutrient digestibility and concentrations of short-chain fatty acids (SCFA) and branched-chain fatty acids (BCFA). Physical pain and wound surface assessment were performed at week 1. Blood was also collected intermittently for measuring biochemical indices and untargeted metabolomics analysis. Results indicated that BW, BCS and daily DMI in CON group increased (P < 0.05) over time after castration, but were maintained relatively stable in CAN group. Cats in CAN group exhibited less pain-related behavior as reflected by lower score of comfort (P < 0.05) and vocalization (P < 0.10), improved wound surface assessment (P < 0.10), lower level of lipase (P < 0.10) and ratio of blood urea nitrogen/serum creatinine (BUN/SC; P < 0.05), and higher level of superoxide dismutase (SOD; P < 0.05) in week 1 than CON cats. Meanwhile, the CAN group had significantly higher concentration of immunoglobulin G (IgG) on days 5 and 7, and higher level of high-density lipoprotein cholesterol (HDL-C; P < 0.10) but lower triglyceride (TG; P < 0.05) than CON group on day 20 and 48. Fecal total and most individual SCFA increased significantly from week 1 to week 6 regardless of diet, but the increase of butyric acid over time only occurred in CON group (P < 0.05). Also, serum metabolomic analysis revealed differential metabolic pathways between the two groups. Overall, compared with the dry food, the canned food tested in our study promoted cat wound recovery by reducing pain and increasing immune and antioxidative capacity after sterilizing surgery, and helped to maintain healthy body condition in cats after castration.

Castration is a surgical operation common in pet cats and dogs, and weight gain is often observed a period after castration. Nutritional management can be important for animal health in both processes. Due to differences in manufacturing techniques and nutrient composition, wet canned food generally exhibits higher palatability and lower energy density than dry food. Till date, few studies have explored if compared to dry kibbles, canned diet can have advantages in promoting recovery from castration surgery and maintaining normal body condition after castration in cats. In our study, dry and canned diets were fed to cats experiencing castration surgery with a free-feeding method. During the one week after surgery, cats fed canned food exhibited less pain and discomfort, and improved inflammation and antioxidative capacity than cats fed dry food. During the four weeks after surgery, cats fed dry food showed significantly more weight gain and change of body condition, meanwhile their blood and fecal measures resembled more of those observed in overweight and/or obese individuals than cats fed canned food. Collectively, canned food with high palatability and low energy density promoted the recovery of cats from the castration surgery and reduced their weight gain after castration.

Effect of Dietary Methylsulfonylmethane Supplementation on Growth Performance, Hair Quality, Fecal Microbiota, and Metabolome in Ragdoll Kittens.

Methylsulfonylmethane (MSM) is a natural sulfur-containing organic substance that has many biological functions, such as antioxidant, anti-inflammatory, skin nourishing, and hair growth-promoting effects. This study was conducted to determine the effect of MSM supplementation on growth performance, antioxidant capacity, and hair quality in kittens. A total of 21 Ragdoll kittens were assigned to three diets by initial body weight and gender: basal diet supplemented with 0%, 0.2%, and 0.4% MSM (CON, LMSM, and HMSM groups) for 65 days. During the whole period, the food intake of kittens in the MSM-treated groups tended to be higher (P < 0.10) compared with the CON group, and the average daily gain (ADG) had no significant difference when compared to the kittens in the CON group (P > 0.05). Antioxidant capacity had no significant difference (P > 0.05) among the groups. The scale thickness of hair tended to be smaller in the LMSM group compared to the CON group (P < 0.10) and decreased significantly (P < 0.05) over time from d 0 to d 65 in the LMSM group, indicating the improvement of hair quality. Besides, supplementation with LMSM increased bacterial diversity. Kittens fed MSM had no significant differences in fecal genus at the end of the study. No significant differences in fecal short-chain fatty acids were observed among groups. Fecal metabolomics analysis further revealed that MSM hardly affected the metabolites. Overall, dietary supplementation with 0.2% MSM can improve the hair quality of kittens. Furthermore, 0.2∼0.4% of MSM had no detrimental effects on serum biochemistry, growth performance, gut microbiota, and metabolome, which supports the safety inclusion of MSM to a certain degree in feline diets. To the best of our knowledge, this is the first study to investigate the effects of MSM supplementation in cats.