[Retracted] MicroRNA­19b promotes the migration and invasion of ovarian cancer cells by inhibiting the PTEN/AKT signaling pathway.

[This retracts the article DOI: 10.3892/ol.2018.8695.].

Modifications on the coastal atmospheric sulfur and cloud condensation nuclei along the Eastern China seas by shipping fuel transition.

Marine fuel combustion from shipping releases SO2 and forms sulfate particles, which may alter low cloud characteristics. A series of strategies were implemented to control the sulfur content of ship fuel oil from 2018 to 2020, offering insights into the effects of the ship fuel oil transition on sulfur-related pollutants and the consequent cloud condensation nuclei (CCN) in the atmosphere. Compared to 2018 in the southeast China waters, shipping SO2 emission decreased by 78 % in 2020, resulting in a 76 % reduction in ship-related total sulfur concentration, and a decrease of 54 % in CCN number concentration under supersaturation 0.2 % (CCN0.2) contributed by shipping. The response of CCN0.2 to ship-related sulfate modification is more pronounced in relatively clean environments than polluted environments, highlighting the uneven changes in coastal CCN along the Eastern China Sea induced by the ship fuel policies. CCN can trigger the formation of cloud droplets, 2020 fuel regulation may have and will reduce the cooling radiative forcing effect with strong spatial heterogeneity. The study provides insights into the variations in coastal atmospheric sulfur-related pollutants and CCN in uneven response to changes in ship fuel oil, prompting the need for further comprehensive assessments of the climate effects resulting from potential shifts in ship fuel use in the future.

Metagenomics reveals the influence of small microplastics on microbial communities in coastal sediments.

The ecological impact of microplastics (MPs) in coastal environments has been widely studied. However, the influence of small microplastics in the actual environment is often overlooked due to measurement challenges. In this study, Hangzhou Bay (HZB), China, was selected as our study area. High-throughput metagenomic sequencing and micro-Raman spectrometry were employed to analyze the microbial communities and microplastics of coastal sediment samples, respectively. We aimed to explore the ecological impact of MPs with small sizes (≤ 100 µm) in real coastal sediment environments. Our results revealed that as microplastic size decreased, the environmental behavior of MPs underwent alterations. In the coastal sediments, no significant correlations were observed between the detected MPs and the whole microbial communities, but small MPs posed potential hazards to eukaryotic communities. Moreover, these small MPs were more prone to microbial degradation and significantly affected carbon metabolism in the habitat. This study is the first to reveal the comprehensive impact of small MPs on microbial communities in a real coastal sediment environment.

Foxo1 Drives the TGFß1-Dependent Dichotomy of Th17 Cell Fates.

T-helper 17 (Th17) cells play a dual role in immunological responses, serving as essential components in tissue homeostasis and host defense against microbial pathogens while also contributing to pro-inflammatory conditions and autoimmunity. While Transforming Growth Factor-beta 1 (TGFß1) is pivotal for the differentiation of non-pathogenic Th17 cells, the role of TGFß3 and Activin in steering Th17 cells toward a pathogenic phenotype has been acknowledged. However, the molecular mechanisms governing this dichotomy remain elusive. In this study, we demonstrate that the transcription factor Foxo1 is upregulated in a TGFß1 dose-dependent manner, serving as a critical regulator that specifically modulates the fate of pathogenic Th17 cells. Analyses in both uveitis patients and an Experimental Autoimmune Uveitis (EAU) mouse model reveal a strong correlation between disease severity and diminished Foxo1 expression levels. Ectopic expression of Foxo1 selectively attenuates IL-17A production under pathogenic Th17-inducing conditions. Moreover, enhanced Foxo1 expression, triggered by TGFß1 signaling, is implicated in fatty acid metabolism pathways that favor non-pathogenic Th17 differentiation. Our drug screening identifies several FDA-approved compounds can upregulate Foxo1. Collectively, our findings offer evidence that Foxo1 serves as a molecular switch to specifically control pathogenic versus non-pathogenic Th17 differentiation in a TGFß1-dependent manner. Suggest that targeting Foxo1 could be a promising therapeutic strategy for autoimmune diseases.

Identification and Parametrization of Key Factors Affecting Levoglucosan Emission During Solid Fuel Burning.

Levoglucosan (LG) is a pyrolysis product of cellulose and hemicellulose at low combustion temperatures. However, LG release cannot be determined only by considering the contents of cellulose and hemicellulose exclusively due to the complexity of combustion processes and the physical-chemical properties of the fuel. This study detected the emission factors (EFs) of LG from 22 different solid fuel samples (including coal and biomass) by considering 18 different fuel properties and five combustion parameters. The average LGEFs during solid fuel burning varied in a range of 0.03-136 mg kg-1, with a magnitude difference of 1-4 orders. While the variations in cellulose (59.5-368 mg g-1) and hemicellulose (73.5-165 mg g-1) contents of fuel samples were only one- to 6-fold. A short combustion duration (<150 min) and a medium combustion temperature (200-400 °C) influenced by volatile and ash contents are crucial for the generation and accumulation of LG. A random forest coupled with the Akaike information criterion stepwise regression model successfully explained 96% of the total LG emission variation using three variables (ash content, cellulose content, and modified combustion efficiency). The ash content promoted coke formation and LG chain cracking by increasing the pyrolysis temperature and is considered the most important factor. The alkali metal in ash can reduce the energy barrier of intramolecular ring contraction reactions and inhibit the dehydration reactions, which led to additional heat being utilized by the competitive pathways of LG formation. This study provided a method to address the parametrization and release mechanisms of combustion source emissions.

Characteristics of atmospheric black carbon and its wet scavenging in Nanning, South China.

Based on in-situ measurement of black carbon (BC) and carbon monoxide (CO), the characteristics of BC emissions and wet scavenging were comprehensively investigated in Nanning, South China. The average annual BC concentration was 1.02 ± 0.53 µg m-3 with higher pollution levels during winter. In winter, a higher net BC/CO (ΔBC/ΔCO) ratio of 3.3 ± 0.3 ng m-3 ppb-1 along with an increased absorption Ångström exponent (AAE) and BC mass from biomass burning (BCbb), indicated a significant contribution of biomass burning to BC emissions. However, emissions from the traffic sector consistently exerted a dominant influence throughout the year. Cluster analysis of backward trajectories identified three types of air masses with distinct origins. Cluster #1 originated from Guangxi province and its vicinity, intermittently influencing the sampling site throughout the year with varying effects between winter and summer. This air mass brought in clean sea breeze in summer whereas transported a higher proportion of BCbb to the site during wintertime due to local open biomass burning. Cluster #3 primarily arrived in autumn and winter (October-December) from polluted central China, resulting in substantially high BC mass at the site. Cluster #2 coincided with the period (January-March) when extensive surface open biomass burning events occurred in Southeast Asia (SEA) regions. These BC aerosols in cluster#2 initially rose to higher altitudes above SEA before being regionally transported, but were significantly scavenged by clouds and precipitation during vertical uplift. The remaining BC exhibited a notably lower BC loss rate on relative humidity (RH) of -0.01 ng m-3 ppb-1 %-1 compared to cluster #1 (-0.03) and cluster #3 (-0.06), corresponding to an average BC transport efficiency of 0.85, 0.73, and 0.53, respectively. Nonetheless, air masses in cluster #2 could still transport considerably high BC mass to Nanning due to dry conditions and less wet scavenging along trajectory pathways. These findings provide valuable insights for policymakers and government officials in regulating and mitigating BC pollution in South China.

Aging effects on residential biomass burning emissions under quasi-real atmospheric conditions.

Emissions from biomass burning (BB) vastly contribute to the atmospheric trace gases and particles, which affect air quality and human health. After emission, the chemical evolution changes the mass and composition of organic aerosol (OA) in the diluted and aged plume. In this study, we used a quasi-real atmospheric smog chamber system to conduct aging experiments and investigated the multiphase oxidation of primary organic aerosol (POA) and the formation of secondary organic aerosols (SOA) in residential biomass burning plumes. We found that the emissions in the gas and particle phases were interlinked during the plume evolution. During photochemical aging, more oxidized OA was produced, and SOA formation increased by a factor of 2 due to functionalization reactions of gaseous precursors such as furans, phenols, and carbonyls. On the other hand, dark aging resulted in a lower OA mass enhancement by a factor of 1.2, with weaker oxidation from gaseous reactions. Dark aging experiments resulted in the generation of substantial quantities of nitrogen-containing organic compounds in both gas and particulate phases, while photochemical aging led to a notable increase in the concentration of gaseous carboxylic acids. Our observations show that the properties of SOA are influenced by exposure to sunlight radiation and oxidants such as OH or NO3 radicals. These results reflect the aging process of BB plumes in real-world atmospheric conditions and highlight the importance of considering various aging mechanisms.

Agricultural management strategies for balancing yield increase, carbon sequestration, and emission reduction after straw return for three major grain crops in China: A meta-analysis.

Straw return can improve crop yield as well as soil organic carbon (SOC) but may raise the possibility of N2O and CH4 emissions. However, few studies have compared the effects of straw return on the yield, SOC, and N2O emissions of various crops. Which management strategies are the best for balancing yield, SOC, and emission reduction for various crops needs to be clarified. A meta-analysis containing 2269 datasets collected from 369 studies was conducted to investigate the influence of agricultural management strategies on yield increase, soil carbon sequestration, and emission reduction in various crops after the straw return. Analytical results indicated that, on average, straw return increased the yield of rice, wheat, and maize by 5.04%, 8.09%, and 8.71%, respectively. Straw return increased maize N2O emissions by 14.69% but did not significantly affect wheat N2O emissions. Interestingly, straw return reduced the rice N2O emissions by 11.43% but increased the CH4 emissions by 72.01%. The recommended nitrogen application amounts for balancing yield, SOC, and emission reduction varied among the three crops, while the recommended straw return amounts were more than 9000 kg/ha. The optimal tillage and straw return strategies for rice, wheat, and maize were plow tillage combined with incorporation, rotary tillage combined with incorporation, and no-tillage combined with mulching, respectively. A straw return duration of 5-10 years for rice and maize and ≤5 years for wheat was recommended. These findings provide optimal agricultural management strategies after straw return to balance the crop yield, SOC, and emission reduction for China's three major grain crops.

Source characterization of volatile organic compounds in urban Beijing and its links to secondary organic aerosol formation.

Volatile organic compounds (VOCs) are precursors for ozone and secondary organic aerosol (SOA) formation, thereby playing a vital role in atmospheric chemistry and urban air quality. To characterize the relationship between VOCs and SOA, organics both in gas and particulate phases were concurrently measured in urban Beijing. The VOCs and organic aerosol (OA) were apportioned into factors with different oxidation levels by applying the factorization analysis on their detailed mass spectra. Six factors of VOCs were identified, including four primary VOCs (PVOC) factors and two secondary VOCs (SVOC) factors. The PVOC factors dominated the total VOCs when the air mass originated in the cleaner northern areas, while SVOC factors dominated for polluted southern air masses. The normalized concentrations of PVOC and primary OA factors showed consistent diurnal variations regardless of air mass directions, owing to the relatively stable local emissions during the experimental period. This contrasted with the secondary factors due to more complex transformation processes. The traffic-related VOCs and solid fuel combustion VOCs negatively correlated with SOA, implying that they may have contributed to the SOA formation through photooxidation. The VOCs in lower oxidation levels were found to have poor correlations with the less oxidized SOA, whereas they correlated strongly to the more oxidized SOA. This implied that the less oxidized SOA may be in a transition state, where its production and loss rates were balanced. These served as products of VOCs oxidation and reactants of more oxidized SOA formation, playing important roles on the VOC to SOA transformation. The identified VOC emission sources and their photochemical production of SOA should be considered in air quality policy planning.

[Investigating the Pollutants of Marine Shipping Emissions Along the East China Sea by Combining in-situ Measurements and Automatic Identification System].

Marine shipping emissions have important impacts on air quality and climate. This type of anthropogenic emission remains largely unclear due to complex vessel types and activities. A coastal site near the Ningbo-Zhoushan port along the East China Sea was selected for this study, representing one of the hotspot regions globally with the most intensive shipping activities, in combination with vessels for both domestic and international transportation. Long-term temporal variations in key gaseous and particulate pollutants were obtained at the site using in-situ measurements, and the vessel speed associated with each classified vessel type was obtained according to the automatic identification system (AIS). In combination of backward trajectories, we were able to identify the periods predominated by the surrounding vessel emissions (in warm seasons, dominated by vessels in full operation or idle mode) or influenced by continental outflow (in cold season). We found that emissions of sulphur dioxide (SO2), nitrogen oxides (NOx), and black carbon (BC) aerosol were highly correlated with high-speed vessels, whereas carbon monoxide (CO) was likely related to lower operation speed. The total particulate matter (PM) was not directly linked to vessel activities. The enhancement factor in operation mode compared to that in idle mode was approximately 1-4 for most pollutants. This direct ambient observation of the emissions from a range of mixed vessel types may provide a basis for evaluating the shipping emission inventory.

Evaluation of the CAMS reanalysis for atmospheric black carbon and carbon monoxide over the north China plain.

Black carbon (BC) and carbon monoxide (CO) at different model levels from the Copernicus Atmosphere Monitoring Service (CAMS) reanalysis were comprehensively evaluated against observations performed simultaneously on both surface and mountain sites in winter and summer in the North China Plain for the first time. CAMS could capture the seasonal difference in BC and CO emission on both sites but showed significant and persistent biases. Biases were high on the surface site and low on the mountain site for both seasons, implying the uncertainties in emission inventories used in the CAMS reanalysis which may have more influence near source. Biases were reduced and the correlation coefficient of CAMS BC with observed BC increased when two datasets were compared on a daily basis, which suggests daily or longer time averaged CAMS BC could be more suitable for trend analysis. Although CAMS could generally reproduce the distinct diurnal variation of BC and CO on both sites, the inaccurate representation of the daily evolution of planetary boundary layer (PBL) in model may bring more uncertainties to the concentration biases on surface from midnight to early morning. BC hydrophilic ratio from CAMS displayed large biases compared to observations with no seasonal difference on both sites, which was probably resulted from the initial emission state of BC hygroscopicity for all source types in model. Uncertainties in the removal processes and the simplified aging processes in model could further induce uncertainty in modelling BC hydrophilic ratio in the CAMS. These results could not only be referenced for the improvement on CAMS reanalysis but also facilitate model or trend analysis of BC and CO pollution by utilizing the CAMS reanalysis product from both short- and long-term perspectives, which will be beneficial to both the mitigation and policy-making on primary emissions in China.

Spatial patterns and driving factor analysis of recommended nitrogen application rate for the trade-off between economy and environment for maize in China.

Appropriate nitrogen (N) application increases crop yield, while its unreasonable application results in environmental problem. Determining the appropriate N application rate is the key to sustainable development. Here, the denitrification-decomposition (DNDC) model was used to analyze the effects of N fertilizer on maize yields, economic benefits, nitrate leaching, and nitrous oxide emissions in China. The N application rate for the trade-off between economy and environment at the county scale was further determined. The geodetector model was used to identify the main driving factors and their interactions of the recommended N rate in each agricultural zone. The results showed that the recommended N rate was generally high in the northwest but low in the south, consistent with the spatial patterns of yield potential. However, clay soils with clay ratios greater than 34% in southern China and sandy soils with bulk densities greater than 1.5 g cm-3 on the Huanghuaihai Plain experienced high N levels and low yields, and thus soils need to be improved. Potential grain yield was the main driving factor in most zones, yet its effects gradually weakened from north to south. The influence of soil characteristics increased from north to south. It was found that the current average N application rate of farmers in China was 249 kg N/ha, and 86.55% of counties had excessive N applications. Compared to the regional optimal N rate at a regional scale, a differentiated N application strategy at the county scale determined in this study increased maize yield and economic benefit by 10.51% and 10.85%, respectively, and reduced N2O emissions and NO3- leaching by 28.72% and 33.60%, respectively. The current research provides a scientific basis for China to formulate a win-win N management strategy for economy and environment and provides a method reference for other countries.

Connecting the Light Absorption of Atmospheric Organic Aerosols with Oxidation State and Polarity.

The light-absorbing organic aerosol (OA) constitutes an important fraction of absorbing components, counteracting major cooling effect of aerosols to climate. The mechanisms in linking the complex and changeable chemistry of OA with its absorbing properties remain to be elucidated. Here, by using solvent extraction, ambient OA from an urban environment was fractionated according to polarity, which was further nebulized and online characterized with compositions and absorbing properties. Water extracted high-polar compounds with a significantly higher oxygen to carbon ratio (O/C) than methanol extracts. A transition O/C of about 0.6 was found, below and above which the enhancement and reduction of OA absorptivity were observed with increasing O/C, occurring on the less polar and high polar compounds, respectively. In particular, the co-increase of nitrogen and oxygen elements suggests the important role of nitrogen-containing functional groups in enhancing the absorptivity of the less polar compounds (e.g., forming nitrogen-containing aromatics), while further oxidation (O/C > 0.6) on high-polar compounds likely led to fragmentation and bleaching chromophores. The results here may reconcile the previous observations about darkening or whitening chromophores of brown carbon, and the parametrization of O/C has the potential to link the changing chemistry of OA with its polarity and absorbing properties.

Effects of black carbon aerosol on air quality and vertical meteorological factors in early summer in Beijing.

Black carbon (BC) aerosols have effects on the atmospheric thermal vertical structure due to its radiation absorption characteristics, hereby influencing the boundary layer characteristics and pollutant diffusion. This study focuses on the BC effects under different atmospheric conditions on air quality and vertical meteorological conditions. Four days flight observation combined with surface wind profiler radar data were used to investigate the vertical profiles of BC and wind speed over Beijing urban area in early summer. The vertical profiles of BC concentration and wind speed in the boundary layer had a negative correlation, both having abrupt changes near the boundary layer height under stagnant weather conditions. The chemical transport model showed the increase of BC under stagnant conditions could cause aggravation of the stability of the boundary layer, thereby increasing the accumulation of pollutants. In particular, BC leads to the changes in the temperature profile, which will modify relative humidity and indirectly lead to the changes in the vertical profile of aerosol optical properties. However, if the early accumulation of BC was absent under more turbulent conditions, the effects of BC on air quality and meteorological conditions were limited.

Assessing the influence of environmental conditions on secondary organic aerosol formation from a typical biomass burning compound.

The atmospheric chemistry in complex air pollution remains poorly understood. In order to probe how environmental conditions can impact the secondary organic aerosol (SOA) formation from biomass burning emissions, we investigated the photooxidation of 2,5-dimethylfuran (DMF) under different environmental conditions in a smog chamber. It was found that SO2 could promote the formation of SOA and increase the amounts of inorganic salts produced during the photooxidation. The formation rate of SOA and the corresponding SOA mass concentration increased gradually with the increasing DMF/OH ratio. The addition of (NH4)2SO4 seed aerosol accelerated the SOA formation rate and significantly shortened the time for the reaction to reach equilibrium. Additionally, a relatively high illumination intensity promoted the formation of OH radicals and, correspondingly, enhanced the photooxidation of DMF. However, the enhancement of light intensity accelerated the aging of SOA, which led to a gradual decrease of the SOA mass concentration. This work shows that by having varying influence on atmospheric chemical reactions, the same environmental factor can affect SOA formation in different ways. The present study is helpful for us to better understand atmospheric complex pollution.

Ammonium Chloride Associated Aerosol Liquid Water Enhances Haze in Delhi, India.

The interaction between water vapor and atmospheric aerosol leads to enhancement in aerosol water content, which facilitates haze development, but its concentrations, sources, and impacts remain largely unknown in polluted urban environments. Here, we show that the Indian capital, Delhi, which tops the list of polluted capital cities, also experiences the highest aerosol water yet reported worldwide. This high aerosol water promotes secondary formation of aerosols and worsens air pollution. We report that severe pollution events are commonly associated with high aerosol water which enhances light scattering and reduces visibility by 70%. Strong light scattering also suppresses the boundary layer height on winter mornings in Delhi, inhibiting dispersal of pollutants and further exacerbating morning pollution peaks. We provide evidence that ammonium chloride is the largest contributor to aerosol water in Delhi, making up 40% on average, and we highlight that regulation of chlorine-containing precursors should be considered in mitigation strategies.

Contrasting resistance of polycyclic aromatic hydrocarbons to atmospheric oxidation influenced by burning conditions.

The oxidation of polycyclic aromatic hydrocarbons (PAHs) determines their lifetime, toxicity and consequent environmental and climate impacts. The residential solid fuel burning composes of a substantial fraction of PAH emissions; however, their oxidation rate is yet to be explicitly understood, which is complicated by the contrasting emission factors under different combustion conditions and their subsequent evolution in the atmosphere. Here we used a plume evolution chamber using ambient oxidants to simulate the evolution of residential solid fuel burning emissions under real-world solar radiation, and then to investigate the oxidation process of the emitted PAHs. Contrasting oxidation rate of PAHs was found to be influenced by particles with or without presence of substantial amount of black carbon (BC). In the flaming burning phase, which contained 46% of BC mass fraction and 8% of organic aerosol (OA) internally mixed with BC, the larger PAHs (with 4-7 rings) was rapidly oxidized 12% for every hour of evolution under solar radiation; however, the larger PAHs from smoldering phase tended to maintain unmodified during the evolution, when 95% of OA was externally mixed with only minor fraction of BC (<5%). This may be ascribed to the complex morphology of BC, allowing more exposure for the internally-mixed OA to the oxidants; in contrast with those externally-mixed OA which was prone to be coated by condensed secondary substances. This raises an important consideration about the particle mixing state in influencing the oxidation of PAHs, particularly the coating on PAHs which may extend their lifetime and environmental impacts.

Prevalence of small-sized microplastics in coastal sediments detected by multipoint confocal micro-Raman spectrum scanning.

Microplastics have become global emerging issue and received widespread attention in recent years. Due to their chemical persistence, plastic particles can be broken into smaller items but accumulated for long time in the environment like sediment. However, limited by current detection technologies, the distribution and characteristics of small-sized microplastics in coastal sediment remain uncertain. In this study, we established a new method based on micro-Raman spectroscopy for detecting small-sized microplastics, namely multipoint confocal micro-Raman spectrum scanning (MCmRSS). The MCmRSS was first applied in detecting microplastics in the sediment samples collected from three bays of the East China Sea. The minimum size of microplastics was 4 µm and average microplastics concentration was 91 ± 55 items /g dry weight sediment, with fragment and polyethylene as the most common shape and polymer type, respectively. The spatial variation of microplastics was in accordance with the strength of coastal human activities and marine dynamics. In all the microplastic items, the small-sized ones (<10 µm) accounted for 67%; and the relationship between microplastic concentration and its size followed a power-exponential equation. Compared with previous studies, the number of microplastics in coastal sediments detected by the MCmRSS increased by 2 orders of magnitude, which was benefited from the advantages of multipoint scanning in the fixed identification areas and high resolution of micro-Raman spectrum. Our findings would summon the re-evaluation of the potential risks of small-sized microplastics in the coastal environment.