We develop and validate a method for the rapid determination and identification of 20 ß-lactamase antibiotics traces in goat's milk by combining the solid phase extraction technology with ultra-high performance liquid chromatography-tandem mass spectrometry. Goat milk samples were extracted with acetonitrile twice. The supernatant was then extracted and cleaned by solid-phase extraction using divinylbenzene and N-vinylpyrrolidone copolymer. The method was validated, with limits of quantification (LOQs) of 0.3 µg kg-1, specificities of 1/3 LOQ, linearities (R2) > 0.99, recoveries of 80-110%, repeatabilities <10.0%, and intermediate precisions <10.0%. The developed method was suitable for the routine analysis of ß-lactamase antibiotics residues in goat's milk and was used to test 76 goat milk samples produced in China.
Anti-Bacterial Agents , Goats , Milk , Solid Phase Extraction , Tandem Mass Spectrometry , beta-Lactamases , Animals , Solid Phase Extraction/methods , Milk/chemistry , Tandem Mass Spectrometry/methods , Anti-Bacterial Agents/analysis , Chromatography, High Pressure Liquid/methods , Limit of Detection , Reproducibility of Results , Drug Residues/analysis , Food Contamination/analysis , Liquid Chromatography-Mass Spectrometry
