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1.
ACS Appl Mater Interfaces ; 16(20): 26547-26556, 2024 May 22.
Article En | MEDLINE | ID: mdl-38727094

Commercial nickel foam (NF), which is composed of numerous interconnected ligaments and hundred-micron pores, is widely acknowledged as a current collector/electrode material for catalysis, sensing, and energy storage applications. However, the commonly used NF often does not work satisfactorily due to its smooth surface and hollow structure of the ligaments. Herein, a gas-phase-induced engineering, two-step gaseous oxidation-reduction (GOR) is presented to directly transform the thin-walled hollow ligament of NF into a three-dimensional (3D) nanoporous prism structure, resulting in the fabrication of a unique hierarchical porous nickel foam (HPNF). This 3D nanoporous architecture is achieved by utilizing the spontaneous reconstruction of nickel atoms during volume expansion and contraction in the GOR process. The process avoids the involution of acid-base corrosion and sacrificial components, which are facile, environmentally friendly, and suitable for large-scale fabrication. Furthermore, MnO2 is electrochemically deposited on the HPNF to form a supercapacitor electrode (HPNF/MnO2). Because of the fully open structure for ion transport, superhydrophilic properties, and the increased contact area between MnO2 and the current collector, the HPNF/MnO2 electrode exhibits a high specific capacitance of 997.5 F g-1 at 3 A g-1 and remarkable cycling stability with 99.6% capacitance retention after 20000 cycles in 0.1 M Na2SO4 electrolyte, outperforming most MnO2-based supercapacitor electrodes.

2.
Langmuir ; 39(32): 11294-11303, 2023 Aug 15.
Article En | MEDLINE | ID: mdl-37534406

As a promising photocatalyst material, g-C3N4 has great application potential in energy production and environmental improvement. In this work, surface-modified g-C3N4 nanosheets with excellent stability and high photocatalytic activity were successfully synthesized by physical steam activation. The charge transfer rate of carbon nitride was improved due to the synergistic effect of nitrogen defect and oxygen doping caused by steam activation. Meanwhile, the specific surface area and pore volume of the optimized sample reached 124.3 m2 g-1 and 0.42 cm3 g-1, respectively, which increased the exposed reaction sites of reactants, enhancing the photocatalytic activity of g-C3N4. In addition, this novel g-C3N4 displayed a great H2 evolution rate of 5889.39 µmol h-1 g-1 with a methylene blue degradation rate up to 6.52 × 10-3 min-1, which was 3.7 and 2.1 times of original g-C3N4, respectively. This study provided a simple and economical method to develop a highly efficient g-C3N4 photocatalyst for solar energy conversion.

3.
Nutrients ; 14(4)2022 Feb 21.
Article En | MEDLINE | ID: mdl-35215553

BACKGROUND: Chronic inflammation is a pathophysiological cause of sarcopenia in Crohn's disease (CD) patients. However, the potential impact of diet-related inflammation on sarcopenia has not yet been adequately investigated. We examined the associations between Dietary Inflammatory Index (DII) and sarcopenia in CD patients. METHODS: A total of 140 CD patients from Ruijin Hospital in Shanghai were included in this cross-sectional study. DII scores were calculated from the dietary data collected using a validated food frequency questionnaire (FFQ). Sarcopenia was determined according to the Asian Working Group for Sarcopenia. Multivariable logistic regression analyses were performed to determine the association between DII and sarcopenia. RESULTS: The mean DII score was 0.81 ± 2.13, ranging from -3.24 to 4.89. The overall prevalence of sarcopenia was 26.4%. The higher DII score significantly increased the risk of sarcopenia in CD patients (ORQuartile4vs1: 9.59, 95% CI: 1.69, 54.42, ptrend = 0.031) in the multivariable model after adjusting for more potential confounders. Moreover, CD patients with a lower DII had a significantly higher appendicular skeletal muscle mass index (ASMI, ORQuartile4vs1: 5.48, 95% CI: 1.51, 19.87, ptrend = 0.018) after adjusting for age, gender, BMI, smoking status and drinking status model. Yet, there were no significant differences between DII and ASMI after adjusting for more potential confounders. Additionally, no significant association was observed between DII and handgrip strength in the multivariable-adjusted models. CONCLUSIONS: Pro-inflammatory diet was associated with increased risk of sarcopenia in CD patients. CD patients should have a proper intake of energy and protein. These patients could also benefit from supplementation with enteral nutrition due to its anti-inflammatory potential.


Crohn Disease , Sarcopenia , China/epidemiology , Crohn Disease/complications , Crohn Disease/epidemiology , Cross-Sectional Studies , Diet/adverse effects , Hand Strength , Humans , Inflammation/epidemiology , Risk Factors , Sarcopenia/epidemiology , Sarcopenia/etiology
4.
Theranostics ; 9(8): 2268-2281, 2019.
Article En | MEDLINE | ID: mdl-31149043

Investigation of targeting inhibitors of Aß aggregation, heme-Aß peroxidase-like activity and efficient detectors of Aß aggregation, are of therapeutic value and diagnostics significance for the treatment of Alzheimer's disease (AD). Due to the complex pathogenesis of AD, theranostics treatment with multiple functions are necessary. Herein we constructed the NIR absorption property of gold nanorods (GNRs) loaded with single chain variable fragment (scFv) 12B4 and thermophilic acylpeptide hydrolase (APH) ST0779 as a smart theranostic complex (GNRs-APH-scFv, GAS), which possesses both rapid detection of Aß aggregates and NIR photothermal treatment that effectively disassembles Aß aggregates and inhibits Aß-mediated toxicity. Methods: We screened targeting anti-Aß scFv 12B4 and thermophilic acylpeptide hydrolase as amyloid-degrading enzyme, synthesized GAS gold nanorods complex. The GAS was evalued by Aß inhibition and disaggregation assays, Aß detection assays, Aß mediated toxicity assays in vitro. In vivo, delaying Aß-induced paralysis in AD model of Caenorhabditis elegans was also tested by GAS. Results: In vitro, GAS has a synergistic effect to inhibit and disassociate Aß aggregates, in addition to decrease heme-Aß peroxidase-like activity. In cultured cells, treatment with GAS reduces Aß-induced cytotoxicity, while also delaying Aß-mediated paralysis in CL4176 C.elegans model of AD. Furthermore, the photothermal effect of the GAS upon NIR laser irradiation not only helps disassociate the Aß aggregates but also boosts APH activity to clear Aß. The GAS, as a targeting detector and inhibitor, allows real-time detection of Aß aggregates. Conclusion: These results firstly highlight the combination of scFv, APH and nanoparticles to be theranostic AD drugs. Taken together, our strategy provides a new thought into the design of smart compounds for use as efficiently therapeutic and preventive agents against AD. Moreover, our design provides broad prospects of biomedical strategy for further theranostics application in those diseases caused by abnormal protein.


Alzheimer Disease/therapy , Hyperthermia, Induced/methods , Nanoconjugates/chemistry , Peptide Hydrolases/metabolism , Phototherapy/methods , Single-Chain Antibodies/chemistry , Theranostic Nanomedicine/methods , Alzheimer Disease/diagnostic imaging , Amyloid beta-Peptides/metabolism , Animals , Caenorhabditis elegans , Cell Line, Tumor , Enzyme Stability , Gold/chemistry , Humans , Infrared Rays , Nanotubes/chemistry , Peptide Hydrolases/chemistry , Peptide Hydrolases/radiation effects , Proteolysis , Smart Materials/chemistry
5.
J Agric Food Chem ; 66(12): 3161-3168, 2018 Mar 28.
Article En | MEDLINE | ID: mdl-29526104

The objective of this study was to investigate whether valine (Val) supplementation in a reduced protein (RP) diet regulates growth performance associated with the changes in plasma amino acids (AAs) profile, metabolism, endocrine, and neural system in piglets. Piglets or piglets with a catheter in the precaval vein were randomly assigned to two treatments, including two RP diets with standardized ileal digestible (SID) Val:Lysine (Lys) ratio of 0.45 and 0.65, respectively. The results indicated that piglets in the higher Val:Lys ratio treatment had higher average daily feed intake (ADFI) ( P < 0.001), average daily gain (ADG) ( P = 0.001), feed conversion ratio (FCR) ( P = 0.004), lower plasma urea nitrogen ( P = 0.032), expression of gastric cholecystokinin (CCK), and hypothalamic pro-opiomelanocortin (POMC). Plasma AAs profiles including postprandial plasma essential AAs (EAAs) profile and in serum, muscle, and liver involved in metabolism of AAs and fatty acids were significantly different between two treatments. In conclusion, Val influenced growth performance associated with metabolism of AAs and fatty acids and both endocrine and neural system in piglets.


Amino Acids/blood , Animal Feed/analysis , Diet, Protein-Restricted/veterinary , Endocrine System/metabolism , Nervous System/metabolism , Swine/growth & development , Valine/metabolism , Animal Nutritional Physiological Phenomena , Animals , Cholecystokinin/metabolism , Dietary Supplements/analysis , Digestion , Endocrine System/drug effects , Female , Ileum/metabolism , Male , Nervous System/drug effects , Pro-Opiomelanocortin/metabolism , Swine/blood , Valine/administration & dosage
6.
J Agric Food Chem ; 64(17): 3371-9, 2016 May 04.
Article En | MEDLINE | ID: mdl-27083206

Nutritional induction of endogenous antimicrobial peptide expression is considered a promising approach to inhibit the outgrowth and infection of pathogenic microbes in mammals. The present study investigated possible regulation of porcine epithelial ß-defensins in response to branched-chain amino acids (BCAA) in vivo and in vitro. BCAA treatment increased relative mRNA expression of jejunal and ileal ß-defensins in weaned piglets. In IPEC-J2 cells, isoleucine, leucine, and valine could stimulate ß-defensin expression, possibly associated with stimulation of ERK1/2 phosphorylation. Inhibition of Sirt1 and ERK completely blocked the activation of ERK and 90RSK protein by isoleucine, simultaneously decreasing defensin expression. BCAA stimulate expression of porcine intestinal epithelial ß-defensins with isoleucine the most, potent possibly through activation of the Sirt1/ERK/90RSK signaling pathway. The ß-defensins regulation of lipopolysaccharide was related with an ERK-independent pathway. BCAA modulation of endogenous defensin might be a promising approach to enhance disease resistance and intestinal health in young animals and children.


Amino Acids, Branched-Chain/chemistry , Amino Acids, Branched-Chain/pharmacology , Intestines/drug effects , Lipopolysaccharides/chemistry , MAP Kinase Signaling System , Sirtuin 1/metabolism , beta-Defensins/metabolism , Animals , Intestinal Mucosa/metabolism , Male , Swine
7.
Anim Sci J ; 87(1): 76-83, 2016 Jan.
Article En | MEDLINE | ID: mdl-26122403

A total of 90 gilts were used to investigate the effects of various standard ileal digestible (SID) total sulfur amino acid (TSAA) to lysine (Lys) ratios on the performance and carcass characteristics of late finishing gilts receiving low crude protein (CP) diets supplemented with crystalline amino acids (CAA). Graded levels of crystalline methionine (Met) (0, 0.3, 0.5, 0.8 or 1.1 g/kg) were added to the basal diet to produce diets providing SID TSAA to Lys ratios of 0.48, 0.53, 0.58, 0.63 or 0.68. At the termination of the experiment, 30 gilts (one pig per pen) with an average body weight (BW) of 120 kg were killed to evaluate carcass traits. Increasing the SID TSAA to Lys ratio increased average daily gain (ADG) (linear and quadratic effect, P < 0.05), improved feed conversion ratio (FCR) (linear and quadratic effect, P < 0.05) and decreased serum urea nitrogen (SUN) concentration (linear and quadratic effect, P < 0.05) of finishing gilts. No effects were obtained for carcass traits. The optimum SID TSAA to Lys ratios to maximize ADG as well as to minimize FCR and SUN levels were 0.57, 0.58 and 0.53 using a linear-break point model and 0.64, 0.62 and 0.61 using a quadratic model.


Amino Acids, Sulfur , Animal Feed , Animal Nutritional Physiological Phenomena , Diet, Protein-Restricted , Dietary Proteins/administration & dosage , Lysine , Methionine/administration & dosage , Swine/physiology , Weight Gain , Animal Feed/economics , Animals , Blood Urea Nitrogen , Crystallization , Female
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