RESUMEN
Here, we present the complete genome sequences of two Zika virus (ZIKV) strains, EcEs062_16 and EcEs089_16, isolated from the sera of febrile patients in Esmeraldas City, in the northern coastal province of Esmeraldas, Ecuador, in April 2016. These are the first complete ZIKV genomes to be reported from Ecuador.
RESUMEN
Following a suspected case of hantavirus in a patientsuffering from acute kidney injury, rodents fromthe patient's property in Yorkshire and the Humber,United Kingdom (UK) were screened for hantaviruses.Hantavirus RNA was detected via RT-PCR in two Rattusnorvegicus. Complete sequencing and phylogeneticanalysis established the virus as a Seoul hantavirus,which we have provisionally designated as strainHumber. This is the first hantavirus isolated from wildrodents in the UK and confirms the presence of a pathogenicSeoul virus in Europe.
Asunto(s)
Lesión Renal Aguda/diagnóstico , Anticuerpos Antivirales/sangre , Infecciones por Hantavirus/epidemiología , ARN Viral/análisis , Virus Seoul/aislamiento & purificación , Lesión Renal Aguda/virología , Animales , Enfermedades Transmisibles Emergentes/virología , Reservorios de Enfermedades , Infecciones por Hantavirus/diagnóstico , Infecciones por Hantavirus/virología , Humanos , Masculino , Datos de Secuencia Molecular , Filogenia , ARN Viral/genética , Ratas , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Enfermedades de los Roedores , Virus Seoul/genética , Análisis de Secuencia de ADN , Reino Unido/epidemiologíaRESUMEN
The largest documented outbreak of Chikungunya virus (CHIKV) disease occurred in the Indian Ocean islands and India during 2004-2007. The magnitude of this outbreak led to speculation that a new variant of the virus had emerged that was either more virulent or more easily transmitted by mosquito vectors. To study this assertion, it is important to know the origin of the virus and how the particular strain circulating during the outbreak is related to other known strains. This study genetically characterized isolates of CHIKV obtained from Mombasa and Lamu Island, Kenya, during 2004, as well as strains from the 2005 outbreak recorded in Comoros. The results of these analyses demonstrated that the virus responsible for the epidemic that spread through the Indian Ocean originated in coastal Kenya during 2004 and that the closest known ancestors are members of the Central/East African clade. Genetic elements that may be responsible for the scope of the outbreak were also identified.
