HPCDNet: Hybrid position coding and dual-frquency domain transform network for low-light image enhancement.

Low-light image enhancement (LLIE) improves lighting to obtain natural normal-light images from images captured under poor illumination. However, existing LLIE methods do not effectively utilize positional and frequency domain image information. To address this limitation, we proposed an end-to-end low-light image enhancement network called HPCDNet. HPCDNet uniquely integrates a hybrid positional coding technique into the self-attention mechanism by appending hybrid positional codes to the query and key, which better retains spatial positional information in the image. The hybrid positional coding can adaptively emphasize important local structures to improve modeling of spatial dependencies within low-light images. Meanwhile, frequency domain image information lost under low-light is recovered via discrete wavelet and cosine transforms. The resulting two frequency domain feature types are weighted and merged using a dual-attention module. More effective use of frequency domain information enhances the network's ability to recreate details, improving visual quality of enhanced low-light images. Experiments demonstrated that our approach can heighten visibility, contrast and color properties of low-light images while better preserving details and textures than previous techniques.

Comparison of anti-ischemic stroke effect of different extraction parts from Gastrodia elata / 中国药房

OBJECTIVE To compare anti-ischemic stroke （IS） effect of different extraction parts from Gastrodia elata， and to provide reference for screening the anti-IS effective parts of G. elata. METHODS G. elata was extracted and separated by ethanol reflux extraction and ethyl acetate extraction. The rat model of diffuse cerebral thrombosis was induced by internal carotid artery injection of arachidonic acid （AA）； the anti-IS effect of G. elata powder， ethanol extract of G. elata， residue of ethanol extract of G. elata， ethyl acetate extract of G. elata， residue of ethyl acetate extract of G. elata， gastrodin and aspirin （positive control drug） were investigated with the content of Evans blue （EB） in the ischemic brain tissue as index. RESULTS Compared with model group， aspirin， ethanol extract of G. elata and ethyl acetate extract of G. elata could significantly decrease the content of EB in the ischemic brain tissue of model rats （P＜0.05）. G. elata powder had the tendency to reduce the content of EB in the ischemic brain tissue of model rats， without statistical significance （P＞0.05）. The residue of ethanol extract of G. elata， residue of ethyl acetate extract of G. elata and gastrodin had little effect on the content of EB in the ischemic brain tissue of model rats. CONCLUSIONS Both ethanol extract of G. elata and ethyl acetate extract of G. elata have anti-IS effects， which are stronger than that of G. elata powder.

A rare mutant of OFD1 gene responsible for Joubert syndrome with significant phenotype variation.

Joubert syndrome (JBTS), a rare genetic disorder resulted from primary cilium defects or basal-body dysfunction, is characterized by agenesis of cerebellar vermis and abnormal brain stem. Both genotypes and phenotypes of JBTS are highly heterogeneous. The identification of pathogenic gene variation is essential for making a definite diagnosis on JBTS. Here, we found that hypoplasia of cerebellar vermis occurred in three male members in a Chinese family. Then, we performed whole exome sequencing to identify a novel missense mutation c.599T > C (p. L200P) in the OFD1 gene which is the candidate gene of X-linked JBTS (JBST10). The following analysis showed that the variant was absent in the 1000 Genomes, ExAC and the 200 female controls; the position 200 Leucine residue was highly conserved across species; the missense variant was predicted to be deleterious using PolyPhen-2, PROVEAN, SIFT and Mutation Taster. The OFD1 expression was heavily lower in the proband and an induced male fetus compared with a healthy male with a wild-type OFD1 gene. The in vitro expression analysis of transiently transfecting c.599T or c.599C plasmids into HEK-293T cells confirmed that the missense mutation caused OFD1 reduction at the protein level. And further the mutated OFD1 decreased the level of Gli1 protein, a read-out of Sonic hedgehog (SHH) signaling essential for development of central neural system. A known pathogenic variant c.515T > C (p. L172P) showed the similar results. All of these observations suggested that the missense mutation causes the loss function of OFD1, resulting in SHH signaling impairs and brain development abnormality. In addition, the three patients have Dandy-Walker malformation, macrogyria and tetralogy of Fallot, respectively, the latter two of which are firstly found in JBTS10 patients. In conclusion, our findings expand the context of genotype and phenotype in the JBTS10 patients.

Application of the plantar horn shaped fasciocutaneous perforator flaps for the reconstruction of the defects on heel and fore plantar / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the application of the plantar horn shaped fasciocutaneous perforator flaps for the reconstruction of the defects on heel and fore plantar.</p><p><b>METHODS</b>The proximal end of the plantar fasciocutaneous perforator flaps were at the edge of defects. The deep fascia was cut off at the both sides of flaps. The plantar fascia should be included into the flaps. Then the flaps were rotated to cover the defects, containing two or three perforators. If necessary, the original supplying vessels could be cut off to facilitate the advancement of flaps.</p><p><b>RESULTS</b>From Jan. 2010 to Oct. 2012, 7 cases with plantar defects were treated with the horn shaped perforator flaps. The defects size ranged from 1.5 cm x 2.0 cm to 4.0 cm x5. 0 cm with the size of the flaps ranging from 7 cm x 3 cm to 13 cm x 7 cm. All the flaps survived completely with primary healing both on donor and recipient sites. The flap color, texture, function and appearance were satisfactory during the follow-up period of 3-24 months.</p><p><b>CONCLUSIONS</b>The plantar horn shaped perforator flap can reconstruct the defects at plantar weight-bearing area with the similar thick skin tissue, which facilitates the functional reconstruction. No skin graft is necessary for the defect at donor site.</p>

[Efficacy of compound xuanju capsule on type-III prostatitis-related sexual dysfunction].

OBJECTIVE: To investigate the value of Compound Xuanju Capsule in the treatment of type-III prostatitis-related sexual dysfunction. METHODS: We randomly divided 90 type-III prostatitis patients with sexual dysfunction diagnosed by NIH clinical criteria into an experiment group and a control group to be treated with Compound Xuanju Capsule and antibiotics, respectively. We analyzed the therapeutic results based on the scores on chronic prostatitis symptom index (CPSI), prostatitis-related sexual function index (PSFI ) and self-rating anxiety scale (SAS), and compared them between the two groups and with the baseline data. RESULTS: The degree of prostatitis-related sexual dysfunction was not correlated with that of prostatitis symptoms. Prostatitis symptoms and sexual function were significantly improved in the experiment group than in the control (P < 0.05), and the SAS score was markedly lower in the former than in the latter (P < 0.05). CONCLUSION: Compound Xuanju Capsule can not only alleviate the symptoms of type-III prostatitis, but also improve its related sexual dysfunction and anxiety.

Application of 1% lauromacrogol in the treatment of facial refractory hemangioma and vascular malformations / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the clinical effect of 1% lauromacrogol for the treatment of facial refractory hemangioma and vascular malformation.</p><p><b>METHODS</b>From Sept 2009 to Nov 2011, 55 patients (20 male, 35 female, 1 month to 30 years) with different types of facial hemangiorwa and vascular malformation about 1.0 cm x (0. 5-5.0) cm x 10.0 cm in size, underwent 1% lauromacrogol intratumor injection therapy. Generally, the injection dose, concentration, frequency were determined by the age of the patients, the volume and depth of the lesion. The dose was limited to 10 mg every time. The injection interval is 14 weeks.</p><p><b>RESULTS</b>After followed up for 3-16 months, 41 cases were cured, 9 cases were greatly improved, and 5 were partially improved. Skin necrosis happened in only 2 cases.</p><p><b>CONCLUSIONS</b>Lauromacrogol is safe, simple and effective as a sderosing agent for the treatment of facial refractory hemangioma and vascular malformation. It provides a new and alternative way for the treatment of facial refractory hemangioma and vascular malformation.</p>

Efficacy of compound xuanju capsule on type-III prostatitis-related sexual dysfunction / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the value of Compound Xuanju Capsule in the treatment of type-III prostatitis-related sexual dysfunction.</p><p><b>METHODS</b>We randomly divided 90 type-III prostatitis patients with sexual dysfunction diagnosed by NIH clinical criteria into an experiment group and a control group to be treated with Compound Xuanju Capsule and antibiotics, respectively. We analyzed the therapeutic results based on the scores on chronic prostatitis symptom index (CPSI), prostatitis-related sexual function index (PSFI ) and self-rating anxiety scale (SAS), and compared them between the two groups and with the baseline data.</p><p><b>RESULTS</b>The degree of prostatitis-related sexual dysfunction was not correlated with that of prostatitis symptoms. Prostatitis symptoms and sexual function were significantly improved in the experiment group than in the control (P < 0.05), and the SAS score was markedly lower in the former than in the latter (P < 0.05).</p><p><b>CONCLUSION</b>Compound Xuanju Capsule can not only alleviate the symptoms of type-III prostatitis, but also improve its related sexual dysfunction and anxiety.</p>

The application of the horn shaped fasciocutaneous flap in lower leg / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore the application of the horn shaped fasciocutaneous flap for the middle or small defect of low leg.</p><p><b>METHODS</b>Between April 2008 and November 2010,12 cases were covered the defects with horn flap in the leg, in which 4 cases were male, and 8 cases were female, such as squamous cell carcinoma, basal cell carcinoma, angioneoplasm, trauma and so on. Surgical repair of tissue defect size of about 3 cm x 3 cm-5 cm x 5 cm.</p><p><b>RESULTS</b>Twelve cases were treated. Vascular deficiency happened at the distal end of one flap. All the other 11 flaps survived. The appearance and functional results were satisfactory with following up for 6 to 24 months.</p><p><b>CONCLUSIONS</b>The horn shaped fasciocutaneous flap is simple, reliable, easy to perform, has a low complication rate, is cosmetically acceptable</p>

[Sequential preparation of microvlllous and basal membranes from human placenta].

OBJECTIVE: To improve the technology of isolating paired fractions of the maternal-facing membranes (MVM) and fetal-facing plasma membranes (BM) from a term placenta. METHODS: The component of buffer was improved based on Illsley method. The time of Mg2+ -aggregated basal membranes was extended. MVM were obtained from the supernatant of low speed centrifugation while BM were further purified on a sucrose step gradient. RESULTS: Yield for MVM and BM prepared by the method were (0.55 +/- 10.10) mg/g and (0.54 +/- 0.02) mg/g wet weight of placenta. They were enriched 16.87-fold and 11.19-fold as determined by the membrane marker enzymes, alkaline phosphatase (MVM) and adenylate cyclase (BM). CONCLUSION: The modified Illsley method can easily produce both MVM and BM of satisfied quantity from human placenta. It could be applied as a cell molecular model of maternal-fetal exchange interface.

Application of modified peroneal tendofascial compound flap with partial tendon of peroneal long muscle in lower leg refractory defects with Achilles tendon rupture / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the feasibility of using modified peroneal tendofascial compound flap with partial tendon of peroneal long muscle in lower leg refractory defect with Achilles tendon rupture.</p><p><b>METHODS</b>Based on the application experience of peroneal tendofascial compound flap for lower leg refractory defects, partial tendon of peroneal long muscle was included in the flap to repair the Achilles tendon rupture.</p><p><b>RESULTS</b>6 cases were treated. The maximum size of modified compound flap reached 5 cm x 5 cm. The maximal included tendon in the flap was 5 cm in length. All the flaps survived completely. Partial epidermis necrosis happened in one case between the skin graft at the donor site and the edge of the flap pedicle. The patients were followed up for 5 months to 2 years with satisfactory cosmetic and functional results.</p><p><b>CONCLUSIONS</b>The modified compound flap has reliable blood supply and can be easily transposed, leaving less morbidity at the donor site. It is very suitable for lower leg refractory defect with the Achilles tendon rupture.</p>

Synthesis and antiviral activities of cyanoacrylate derivatives containing an alpha-aminophosphonate moiety.

Target compounds 8 were obtained by the reaction of alkyl 2-cyano-3,3-dimethylthioacrylate or cyarylamide (7a-7e) and alpha-aminobenzylphosphonate (5a-5e) under reflux condition using ethanol as solvent. Their structures were clearly verified by spectroscopic data (IR and 1H, 13C, and 31P NMR) and elemental analysis. These compounds were shown to be antivirally active in the bioassay. It was found that title compounds 8d and 8e had the same inactivation effect against tobacco mosaic virus (EC 50 = 55.5 and 55.3 microg/mL) as the commercial product ningnanmycin (EC 50 = 50.9 microg/mL). To the best of our knowledge, this is the first report on the synthesis and antiviral activity of cyanoacrylate derivatives containing an alpha-aminophosphonate moiety.

Application of modified reversed neurovascular flaps nourished by cutaneous nerve-superficial vein in lower extremity / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the application and therapeutic effect of modified neurovascular flaps nourished by sural nerve and small saphenous venous, or saphenous nerve and great saphenous venous.</p><p><b>METHODS</b>According to the anatomy and recipient area, we made some modifications for the two neurovascular flaps. 1) The small and great saphenous venous were ligated or anastomosed with the venous in recipient area; 2) The flap was delayed for 8 - 14 days if the donor area was above the middle and upper one third of leg; 3) The sural nerve and its branch were anastomosed to the nerve in recipient site to restore the sense of flap; 4) the lower turning point could be designed just 3 cm above the point of internal and external malleolus; 5) Partial gastrocnemius or soleus muscle could be included in the flap if needed; 6) Reversed cross leg flap was considered if the adjacent flap in the affected side could not be used. Sometimes, we adopted more than one modification in one patients.</p><p><b>RESULTS</b>From Sep, 1994 to Apr, 2007, 63 cases were treated, including 51 cases of sural and 12 cases of saphenous neurovascular flaps. Partial necrosis (about 1 - 2 cm width) happened in the distal end of flaps in 2 cases which healed after dress changing. The patients were followed up for 3 months to 2 years with satisfied functional and cosmetic results.</p><p><b>CONCLUSIONS</b>These two modified flaps have reliable blood supply and survival rate. The operation is easily performed and very practical. The flaps, or combined with other methods can repair about 70% of the refractory defects in legs.</p>

Anatomic study of peroneal tendofascial flap combined with adipofascial flap for the repair of heel tissue defects / 中华整形外科杂志

<p><b>OBJECTIVE</b>To study the anatomy of peroneal tendofascial flap combined with adipofascial flap for the repair of heel tissue defects.</p><p><b>METHODS</b>The lower extremities of five cadavers (10 sides) were perfused with red latex, the blood supply of peroneal tendofascial flap and vicinity adipofascial flap were observed. The diameter, course, branches and location of the blood vessels were measured. Eight fresh cadavers (16 sides) were perfused with lead oxide-gelatine mixture. The covering fascia tissues of the lower extremities was obtained and photographed by X-ray. The vascular anastomosis and association of nutrient vessel of peroneal tendofascial flap and vicinity adipofascial flap were observed. Two adult lower extremities specimens (4 sides) were used to construct vessel diagrams for observation of the course, distribution and anastomosis of the vessels. Eight cases were treated successfully with theses flaps.</p><p><b>RESULTS</b>The blood supply of the combined fascial flap is multi-originated. For the area within 4 cm below and above the lateral malleolus cusp, the blood supply includes 2-5 branches from heel lateral artery with an average diameter of (0.5 +/- 0.2) mm, 1-2 branches from posterior lateral malleolus artery with an average diameter of (0.6 +/- 0.2) mm and 2-3 branches from the descending part of perforating branches of peroneal artery with an average diameter of (0.5 +/- 0.2) mm. The blood supply of area 4 cm above lateral malleolus cusp is 1-3 branches from intermuscular septum perforating branches of peroneal artery with an average diameter of (1.0 +/- 0.2) mm. These above branches are anastomosed each other and also send off many smaller branches to form vascular net around tendon. The fascial flaps and free skin grafts in eight patients were completely survived. All patients were followed up for 3-24 months, the donor and recipient sites were healed very well. The functional and cosmetic results were satisfactory.</p><p><b>CONCLUSIONS</b>Peroneal tendofascial flap combined with adipofascial flap, with proximal pedicle or reverse distal pedicle, can be used to repair the defect at the lower leg and refractory small- and medium-sized defects at the heel.</p>

Heel deep tissue defects were repaired by perineal tendofascial flap conjoined with adipofascial flap / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore a new method and estimate its outcome to repair deep heel tissue injuries with local tissue flap.</p><p><b>METHODS</b>At lower cnemis, a fat fascial flap combined with perineal tendofascial flap were designed and elevated between the lateral malleolars blank, the largest area is 6 cm x 13 cm, which were transplanted to cover naked calcaneal tendon and calcaneal bone, full-thickness free skin graft or middle-thickness free skin graft were grafted on flap.</p><p><b>RESULTS</b>6 patients suffered from heel tissue defects had been treated from January 2004 to November 2005. The smallest area was 3.5 cm x 4.0 cm and the largest was 4 cm x 6 cm. All fascial flaps and free skin grafts were successfully survived after operations. All patients were followed up 3-23 months, the operation areas were healed, the functions and configurations were satisfied.</p><p><b>CONCLUSIONS</b>Perineal tendofascial flap conjoined with adipofasical flap and free skin graft are used to repair heel deep tissue defects, which is a handy, dependable way for repairing of the small tissue defects of the lower third of leg and heel.</p>

The thoracodorsal artery musculocutaneous perforator flap: anatomic study and clinical significance / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the feasibility of clinical application of the thoracodorsal artery musculocutaneous perforator flap (TAMPF).</p><p><b>METHODS</b>(1) The morphosis and blood supply of TAP flap on 15 formalized adult cadavers(30 sides) were examined by microsurgery anatomy. (2) An imitative operation of the TAMP flap and latissimus dorsi flap on 1 formalized adult cadavers (2 sides) was conducted.</p><p><b>RESULTS</b>(1) A total of 102 musculocutaneous perforators larger than 0.5 mm were found in 16 specimens(32 sides). 56 perforators (55%) were originated from the medial branch and 46 (45%) originated from the lateral branch. The biggest perforator is (0.82 +/- 0.11) mm (0.68 - 1.08 mm). There was an average of 1.9 perforators (range, 1 - 3 perforators) of the medial branch and an average of 1.8 perforators (range, 1 - 3 perforators) of the lateral branch. Additionally, there were 24 perforators samller than 0.5 mm, and 76 perforators originated from intercostal artery and lumbar artery. (2) Musculocutaneous perforators over 0.5 mm were found only in proximity of the medial and lateral branches within a distance of 8.5 cm (6.4 cm - 9.2 cm) distal to the neurovascular hilus.</p><p><b>CONCLUSIONS</b>With the characteristics of constant position, large caliber, long pedicle, the thoracodorsal artery musculocutaneous perforator was suitable to be musclocutaneous perforator flaps and "fan-shaped" flaps.</p>

Application of minimal access in spinal surgery / 上海第二医科大学学报

Minimal access spinal surgery(MASS),a new minimally invasive technique,has been developed on the basis of classic minimally invasive spinal surgery(MISS).With some special equipments,this procedure can reduce approach-related morbidity and collateral damage to surrounding tissues,and can be performed under direct visualization of the spine.The MASS combines the merits of MISS and traditional open procedures,and comparable results can be obtained while decreasing approach-related complications and learning curve.Now,the application of MASS has become more frequent in clinical practice due to its favourable characteristics in curative effect,postoperative recovery and economic efficacy.

Nasal reconstruction with the modified expanded forehead skin flap / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the effect of the modified expanded forehead skin flap in nasal reconstruction.</p><p><b>METHODS</b>According to flap design, the frontal arteries that were not selected as the pedicle were ligated in order to enhance expansion and delay effects. Besides the middle forehead skin flap for nasal reconstruction, the expanded transversal forehead flap was employed with its donor site sutured directly, resulting in inconspicuous scar. This method was used for 11 cases of nasal reconstruction.</p><p><b>RESULTS</b>All the flaps survived. Postoperative follow up for 6 months to 8 years and 4 months showed satisfactory results with good appearance and function.</p><p><b>CONCLUSIONS</b>The method of modulating the blood supply to the flap and selecting the upper area of the forehead for the flap is an effective modification for nasal reconstruction.</p>

An improved trilinear decomposition algorithm based on a Lagrange operator.

An improved trilinear decomposition algorithm based on a Lagrange operator (LO) is developed in this paper, which introduces a Lagrange operator and penalty terms in the loss function to improve the performance of the algorithm. Compared to the traditional parallel factor (PARAFAC) algorithm, the algorithm not only may converge much faster, but also overcome the sensibility to estimate the number of components. A set of simulated and measured excitation/emission fluorescence data were treated by both the proposed and traditional PARAFAC algorithm to compare their efficiencies. The analytical results obtained with real chemical system containing aspirin and its metabolic products show that the trilinear decomposition methodology is a promising tool to obtain spectral and composition information from mixtures without chemical separation.

Preauricular lateral maxillocervical fasciocutaneous flap / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the design and blood supply type of the reverse narrow pedicle lateral maxillocervical fasciocutaneous flap.</p><p><b>METHODS</b>The reverse narrow pedicle lateral maxillocervical fasciocutaneous flap with its pedicle locate in front of auricle was designed to repair the defect caused by removing the malignant tumor in aged patients. The largest size is about 10 cm x 6 cm, the width of pedicle ranged from 2.5-3.0 cm, 2-4 cm in length.</p><p><b>RESULTS</b>The flaps were used in 4 patients, age from 57 to 83 years old and the flap was designed as lateral maxillocervical fasciocutaneous flap. The flap was survived well in all patients, donor site can be closed without strain, no tumor relapsed.</p><p><b>CONCLUSION</b>The flap should be included to be a reverse axial flap and it should be designed from jaw edge to chin. The pedicle couldn't be too narrow, in this way, donor site can be closed directly, and less surgical operation time needed. The patients can accept synthesize treatment early, for example actinotheraphy. It is an ideal choice for repair the defect caused by removing the malignant tumor in aged patients.</p>