[Comparative transcriptomics analysis of Citrus medica var. sarcodactylis at different developmental stages].

In order to study the transcriptional differences of Citrus medica var. sarcodactylis at different developmental stages, we explored the genes regulating the biosynthesis of the effective components. In this study, Illumina Hiseq 4 000 high-throughput sequencing technology was used to sequence the transcriptome of C. medica var. sarcodactylis at different developmental stages, 121 235 unigenes were obtained with an average length of 2 434 bp, 3 379 different genes were obtained using DESeq screening, which mainly connected to biological processes such as signal transmission, biological regulation, and metabolic processes, and enriched in metabolic pathways such as starch, sucrose metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis. Further dynamic comparison of biosynthesis related genes of active ingredients: the expression levels of PAL, CHI, CYP75B1, ZDS, 4CL and FLS gradually increased as the fruit turned from green to yellow; the expressions of COMT, F3H and CYP73A increased at first and then decreased; CCR, HCT and HRP were down-regulated whereas up-regulated. This study provides references for further excavation of key genes in the biosynthesis of active components, as well as biopathway analysis of active components for C. medica var. sarcodactylis.

[Morphological and histological studies of Herpetospermum pedunculosum seeds and other substitutes].

OBJECTIVE: To provide new evidences for the identification of Herpetospermum pedunculosum seeds and other substitutes. METHOD: Microscopic examination of the cross sections and powers of the seeds of H. pedunculosum, Momordica charantia, Thladiantha setispina and M. cohinchinensis were made to characterize the drug materials. RESULT AND CONCLUSION: The external and internal structure of T. setispina and M. charantia were studied for the first time. Two types of different branched starry sclereids was found in M. cohinchinensis. Comparative studies on external and internal structure were carried out on seeds of the 4 species. As a result, they could be distinguished by external characteristics such as shape, size and color of their seeds, and by internal structure such as size and type of sclereid, epidermis and sub-epidermis cell of seed coat.