Role of extracted phytochemicals from Rosa sterilis S. D. Shi in DSS-induced colitis mice: potential amelioration of UC.

Rosa sterilis S. D. Shi is a new variety of R. roxburghii Tratt and its fruits are rich in bioactive components, but its effects and mechanisms against intestinal inflammation are currently unknown. In this study, the main components of the ethanol extract of R. sterilis S. D. Shi fruits (RSE) were identified and its anti-inflammatory efficacy in DSS-induced mice was evaluated. A total of nine compounds were identified, including 1-O-E-cinnamoyl-(6-arabinosylglucose), ellagic acid-O-rhamnoside, (epi) catechin, niga-ichigoside F1, etc. The results demonstrated that RSE ameliorated DSS-induced inflammation in mouse colon tissues by increasing mucin expression, reducing the production of TNF-α, IL-1ß, and IL-6, inhibiting the mRNA expression of COX-2 and iNOS, regulating the composition of gut microbiota through suppressing Escherichia-Shigella while increasing Akkermansia muciniphila, and promoting the production of SCFAs, especially acetic acid. Briefly, RSE showed outstanding potential for anti-inflammatory activity and is expected to be a promising dietary supplement for healthy individuals to prevent or relieve colitis and colitis-related diseases, which provided a new direction for functional food development.

A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods: Structural and in vitro fermentation characterizations.

In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time. The results indicated that extraction methods exhibited significant effects on the structure of RSPs, thus resulting in different probiotic effects. HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA. EM-RSP was rich in RG-I and its size of average side chain were the largest. Moreover, HW-RSP and AK-RSP exhibited the smallest (57.55 kDa) and largest (922.20 kDa) molecular weights, respectively. All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota. Furthermore, the function prediction results showed that EM-RSP had the most special metabolic pathways. Collectively, our findings provide new insights into the relationship between the structure and probiotic function of RSPs, and offer theoretical basis for the development of functional products of Rosa sterilis S.D.Shi.

Beneficial effects of high pressure processing on the interaction between RG-I pectin and cyanidin-3-glucoside.

In the present study, the effects of high-pressure processing (HPP) on the binding capacity, structural properties, antioxidant capacity and stability of rhamngalacturonan I (RG-I) pectin and cyanidin-3-glucoside (C3G) were investigated. HPP was found to have a positive effect on the binding between the two molecules, and the binding rate was increased by 32.8% after treatment (500 MPa/15 min). The increase in the binding rate is mainly due to the influence of high pressure on noncovalent effects such as hydrogen bonding and hydrophobicity. The results indicate that high pressure can be used to maintain the antioxidant capacity of C3G and improve the stability of C3G. The C3G retention rate is increased by 34.0% and 38.3% after heat treatment and simulated digestion, respectively. The results indicate that HPP improved stability of the C3G-RG-I pectin complex through interaction between C3G and RG-I pectin.