Clinical efficacy of different therapeutic strategies in patients with spontaneous rupture of the esophagus: a multicenter retrospective cohort study.

BACKGROUND: The therapeutic strategy for patients with spontaneous rupture of the esophagus includes surgical repair, endoscopic therapy, supportive care, and others. However, no evidence exists to direct clinical decision-making regarding the choice of operative and nonoperative management. The aim of this study was to determine the clinical efficacy of different therapeutic strategies in both general and stratified patients. METHODS: This study retrospectively analyzed a consecutive cohort of 101 patients at nine tertiary referral hospital centers in China. Patients were divided into operative and nonoperative groups based on the initial treatment. Short-term outcomes, including 90-day mortality, length of hospital stay, and postoperative leakage were compared. Subgroup analysis was performed based on treatment timing and Pittsburgh perforation severity score (PSS). RESULTS: Of 101 patients, 60 (58.4%) underwent operative management. A significant difference of 90-day mortality between operative and nonoperative groups was observed (15.0% vs. 34.1%, P=0.031). Operative management tend to yield similar therapeutic benefits in timely (OR, 0.250; 95% CI, 0.05-1.14, P=0.073) and delayed (OR, 0.42; 95% CI, 0.12-1.47, P=0.175) treatment groups. Based on PSS stratification, operative management significantly decreased the risk of 90-day mortality (OR, 0.211; 95% CI, 0.064-0.701; P=0.011) for patients in low- and moderate-risk groups but may be detrimental for patients in high-risk group (OR, 1.333; 95% CI, 0.233-7.626; P=0.746). CONCLUSIONS: Operative management might be superior to nonoperative management for low- and moderate-risk patients with spontaneous rupture of the esophagus. However, for patients at high risks, operative management might not provide additional benefits compared with nonoperative management. Further research involving larger sample sizes is required for accurate patient stratification and conclusive evidence-based guideline.

Double-drying 3D lamellar-structured aerogel membrane for efficient oil-water separation and long-lasting antibacterial activity.

Conventional oil-water separation membranes are difficult to establish a trade-off between membrane flux and separation efficiency, and often result in serious secondary contamination due to their fouling issue and non-degradability. Herein, a double drying strategy was introduced through a combination of oven-drying and freeze-drying to create a super-wettable and eco-friendly oil-water separating aerogel membrane (TMAdf). Due to the regular nacre-like structures developed in the drying process and the pores formed by freeze-drying, TMAdf aerogel membrane finally develops regularly arranged porous structures. In addition, the aerogel membrane possesses excellent underwater superoleophobicity with a contact angle above 168° and antifouling properties. TMAdf aerogel membrane can effectively separate different kinds of oil-water mixtures and highly emulsified oil-water dispersions under gravity alone, achieving exceptionally high flux (3693 L·m-2·h-1) and efficiency (99 %), while being recyclable. The aerogel membrane also displays stability and universality, making it effective in removing oil droplets from water in corrosive environments such as acids, salts and alkalis. Furthermore, TMAdf aerogel membrane shows long-lasting antibacterial properties (photothermal sterilization up to 6 times) and biodegradability (completely degraded after 50 days in soil). This study presents new ideas and insights for the fabrication of multifunctional membranes for oil-water separation.

AhR agonists screening and identification in indoor dust based on non-target chemical analysis by GC-Q-TOFMS and biological effect evaluation referring to ToxCast/Tox21 database.

Numerous studies reported the concentration of agonists of aryl hydrocarbon receptor (AhR) in indoor dust by target chemical analysis or the biological effects of activating the AhR by indoor extracts, but the major AhR agonists identification in indoor dust were rarely researched. In the present study, the indoor dust samples were collected for 7-ethoxyresorufin O-deethylase (EROD) assay and both non-targeted and targeted chemical analysis for AhR agonists by gas chromatography quadrupole time-of-flight mass spectrometry and gas chromatography-mass spectrometry analysis. Coupled with non-targeted analysis and toxicity Forecaster (ToxCast)/Tox21 database, 104 ToxCast chemicals were screened to be able to induce EROD response. The combination of targeted chemical analyses and biological effects evaluation indicated that PAHs, dibutyl phthalate (DBP) and Cypermethrin might be the important AhR-agonists in different indoor dust and mainly contributed in 1.84%-97.56 % (median: 26.62%) of total observed biological effects through comparing toxic equivalency quotient derived from chemical analysis with biological equivalences derived from bioassay. DBP and cypermethrin seldom reported in the analysis of AhR agonists should raise great concern. In addition, the present results in experiment of synthetic solution of 4 selected AhR-agonists pointed out that some unidentified AhR agonists existed in indoor dust.

ATGL promotes colorectal cancer growth by regulating autophagy process and SIRT1 expression.

CRC is a common malignant tumor in the gastrointestinal tract, and its incidence has increased significantly in recent years. Several studies revealed that lipid metabolism reprogramming contributed to tumorigenicity and malignancy by interfering with energy production, membrane formation, and signal transduction in cancers. ATGL is a kind of hydroxy fatty acid ester of fatty acid synthase, and its role in tumor remains controversial. We compared levels of adipose triglyceride lipase (ATGL) in human CRC specimens to adjacent specimens. To validate the effect of ATGL on the proliferation ability of CRC, CCK8 assay and clone formation assay were performed. To evaluate whether autophagy process takes part in the effect of ATGL on CRC proliferation, the value of LC3-II/LC3-I was detected by western blot and we blocked the SIRT1 to detect value of LC3-II/LC3-I and p62 via western blot. In the end, we detected the value of SIRT1 in CRC specimens. We found that ATGL showed high expression in CRC and positively correlated with clinical stage, indicating poor prognosis of CRC. Moreover, ATGL significantly promoted tumor cell proliferation in vitro. Mechanistically, ATGL promoted CRC cells proliferation by blocking mTOR signaling pathway and activating autophagy process. Further, ATGL regulated autophagy process through triggering SIRT1 expression. Our results reveal that ATGL promotes colorectal cancer growth by up regulating autophagy process and SIRT1 expression.

Inhalation bioacessibility and lung cell penetration of indoor PM2.5-bound PAEs and its implication in risk assessment.

Several studies have evaluated the human exposure of phthalate esters (PAEs) in PM2.5 via inhalation route, however, inhalation bioaccessibility and the lung cell penetration of PAEs were barely considered in risk assessment. In the present study, PM2.5 samples collected from indoor environments were investigated for inhalation bioaccessibility of PAEs using two simulated lung fluids (gamble's solution (GMB) and artificial lysosomal fluid (ALF)). The results showed that the inhalation bioaccessibility of PAEs (except for diethyl phthalate) under healthy state (GMB: 8.9%-62.8%) was lower than that under the inflammatory condition (ALF: 14.5%-67.6%). Lung cell permeation and metabolism of three selected PAEs (diethyl phthalate, di(n-butyl)phthalate and di-2-ethylhexyl phthalate) was tested using equivalent lung cell (A549) model. The inhalation bioavailability obtained by combination of the bioaccessibility of PAEs in indoor PM2.5 and permeability data of A549 cell ranged from 11.7% to 51.1% in health condition, and 13.5%-55.0% in inflammatory state. The calibration parameter (Fc) based on the inhalation bioavailability was established in present study and could provide a reference for a more accurate risk assessment of PM2.5-bound PAEs.

Synthesis of Xylan-Click-Quaternized Chitosan via Click Chemistry and Its Application in the Preparation of Nanometal Materials.

For the high-valued utilization of hemicelluloses and for realizing the controllable synthesis of NPs, this paper's aim is to combine xylan, chitosan and nanometal materials at the same time. In this research study, firstly, propargyl xylan was synthesized via nucleophilic substitution reaction between xylan and propargyl bromide in NaOH solution. On the other hand, a tosyl group was introduced onto the 6th position of synthesized quaternized chitosan (QCS), and the azide group replaced the tosyl group to obtain 6-amido-QCS (QCS-N3). The synthesis conditions of the above reactions were optimized. Subsequently, the novel xylan-click-QCS polymer was obtained via click reaction between terminal alkyne groups on the xylan chains and azide groups on QCS. Then, AgNPs and AuNPs were synthesized by adopting the xylan-click-QCS polymer as the reducing and stabilizing agent, and the reaction conditions were optimized to obtain well-dispersed and highly stable nanoparticles. There were two kinds of Ag nanomaterials, with diameters of 10~20 nm and 2~5 nm, respectively, indicating the formation of Ag nanoclusters, except for Ag nanoparticles, in this reaction. The diameter of the synthesized AuNPs was 20~30 nm, which possessed a more uniform size distribution. The Ag nanoclusters with a smaller size (2~5 nm) could inhibit MCF-7 cell proliferation effectively, indicating their application potential in cancer therapy. The study gives a new approach to the high-value utilization of biopolymers.

Adsorption of levofloxacin on natural zeolite: effects of ammonia nitrogen and humic acid.

The persistence of antibiotics in sewage treatment plants in recent years has become a serious problem. Meanwhile, humic acid and ammonia nitrogen are widely distributed in natural reservoirs and might influence the sorption, migration and transformation of antibiotics. In this study, natural zeolite (NZ) was evaluated as an adsorbent for the removal of levofloxacin (LEV). The physical and chemical properties of NZ before and after adsorption were characterized by various analytical techniques to develop the mechanism. The effects of ammonia nitrogen and humic acid (HA) on the interfacial behavior of LEV on NZ were explored. Comparative experiments revealed that LEV adsorption on NZ involved electrostatic interactions and ion exchange, and the adsorption processes were well fitted by the Langmuir isotherm model and pseudosecond-order kinetic model. The maximum experimental adsorption capacity of LEV was 22.17 mg·g-1 at pH 6.5. The presence of ammonia nitrogen and HA significantly suppressed the adsorption of LEV due to competitive adsorption, and the adsorption capacity decreased 58 and 46%, respectively. It is obvious that low concentrations of ammonia nitrogen and HA are conducive to improving the treatment effect of sewage. This study demonstrates that NZ is a promising and efficient material for LEV adsorption.

Study on sustainable developments in Guangdong Province from 2013 to 2018 based on an improved ecological footprint model.

Aiming at the ecological footprint model, the traditional trade adjustment method only considered the international trade process at the urban scale, ignoring the trade footprint generated by domestic trade and indirect trade in various products. This paper adopts the urban-scale ecological footprint model based on the macro-trade adjustment method to calculate the trade adjustment coefficient of biological products and the energy trade adjustment coefficient respectively to correct the trade footprint. The results showed that the per capita ecological deficit showed a straight upward trend, from 0.07351 hm2 in 2013 to 0.15472 hm2 in 2018. From 2013 to 2018, the per capita ecological footprint of Guangdong Province was greater than the per capita ecological carrying capacity, and the ecological economic system of Guangdong Province was in an unsustainable state. According to the trade ecological footprint, Guangdong Province was a completely foreign resource and service exporting city, which was consistent with Guangdong Province's own economic development direction; the analysis results of the ecological product trade footprint were more consistent with the current city positioning of biological resource products of each city, and the energy indirect trade footprint. The improved ecological footprint model could more accurately assess the true status of ecological vitality above the urban scale.

Correction: Influence of the shared epitope on the elicitation of experimental autoimmune arthritis biomarkers.

[This corrects the article DOI: 10.1371/journal.pone.0250177.].

Recent Progress in Ti3C2Tx MXene-Based Flexible Pressure Sensors.

The rapid development of consumer electronics, artificial intelligence, and clinical medicine generates an increasing demand for flexible pressure sensors, whose performance depends significantly on sensitive materials with high flexibility and proper conductivity. MXene, a type of 2D nanomaterial, has attracted extensive attention due to its good electrical conductivity, hydrophilicity, and flexibility. The synthesis methods for MXenes make it relatively easy to control their microstructure and surface termination groups. Hence, MXenes can obtain peculiar microstructures and facilely combine with other functional materials, making them promising prospects for use in flexible pressure sensors. In this Review, recent advances in MXenes are summarized, mainly focusing on the synthesis methods and their application in flexible pressure sensors. Finally, the challenges and potential solutions for future development are also discussed.

Antibacterial chitosan composite films with food-inspired carbon spheres immobilized AgNPs.

The cumulative toxicity of AgNPs has limited their application in food packaging. As such, the quest for AgNPs should focus on controlling their release to reduce the cumulative toxicity. Here, two kinds of green hydrothermal carbonized methods were used to treat sulfhydryl-modified chitosan to obtain two kinds of carbon spheres/AgNPs (Glutinous rice sesameballs-like AgNPs-SMCS and dragon fruit-like SMCS-Ag), which exhibited good stability and high immobilization efficiency for AgNPs, and the release of total Ag from AgNPs-SMCS and SMCS-Ag were only about 5.63% and 3.59% after 14 days, respectively. Subsequently, they were added into chitosan separately to prepare chitosan-based films. Two carbon spheres/AgNPs regulated the microstructure of chitosan-based films because of the electrostatic interaction and the micro-nanometer filling behavior, thus further immobilized the AgNPs. Importantly, the films presented good antibacterial activity and excellent safety. These results will provide a theoretical basis for the green and safe design of AgNPs antibacterial agent.

Influence of the shared epitope on the elicitation of experimental autoimmune arthritis biomarkers.

Our previous studies have shown that inoculation of the oral cavity of "humanized" B6.DR1/4 mice with the periodontal pathogen Porphyromonas gingivalis results in an increase in the percentage of circulating Th17 cells, loss of bone and an exacerbation of experimental autoimmune arthritis. The aim of this study was to assess the role played by the human HLA-DRß molecule containing the shared epitope supplied as a transgene to I-A˚ (murine class II null) C57BL/6 (B6) mice in driving these findings. We compared various immune response parameters as well as alveolar and peri-articular bone loss between humanized B6.DR1 (or B6.DR4) mice and their WT (B6) counterparts. We found that the presence of the shared epitope in the context of inoculation with P. gingivalis enhanced the percentage of Th17 cells generated, dramatically enhanced bone loss and importantly allowed for the generation of CCP2⁺ ACPAs that are not found in C57BL/6 or DBA/1 arthritic mouse serum. Due to the exceedingly complex nature of environmental factors impacting on genetic elements, it has been difficult to unravel mechanisms that drive autoimmune arthritis in susceptible individuals. The findings in this study may provide one small piece of this puzzle that can help us to better understand part of this complexity.

Inhalation bioacessibility and absorption of polycyclic aromatic hydrocarbons (PAHs) in indoor PM2.5 and its implication in risk assessment.

Inhalation bioaccessibility of polycyclic aromatic hydrocarbons (PAHs) in PM2.5 was assessed in numerous studies, however, the lung cell uptake and penetration of PAHs was seldom taken into account in risk assessment. In the present study, eighteen indoor PM2.5 samples collected from Guangzhou, China were analyzed for the inhalation bioavailability of PAHs combining the inhalation bioaccessibility and cell absorption of PAHs. Two simulated epithelial lung fluid mimicking the healthy condition (as represented by gamble's solution (GMB), pH = 7.4) and the inflammatory condition (as represented by artificial lysosomal fluid (ALF), pH = 4.5) were employed to evaluate the inhalation bioaccessibility. The results indicated that the bioaccessibility of PAHs under the inflammatory condition (1.28%-87.7%) was higher than that under healthy condition (0.88%-87.6%). Naphthalene, phenanthrene, pyrene and benzo[a]pyrene were selected for absorption assay of lung epithelial cells (A549). The absorption rate of PAHs ranged from 64.7 to 90.7% and it was inversely proportional to the number of aromatic rings. Taken together, the inhalation bioavailability based on the bioaccessibility of PAHs and the lung cell absorption ratio ranged from 9.9 to 56.9% under the healthy state, from 12.7 to 65.6% under inflammatory condition. The correction parameter (Fc) was thus established and can be used to improve the risk assessment of human exposure to PAHs via PM2.5 inhalation in future work.

Latest Advances on Bacterial Cellulose-Based Antibacterial Materials as Wound Dressings.

At present, there are various wound dressings that can protect the wound from further injury or isolate the external environment in wound treatment. Whereas, infection and slow self-healing still exist in wound healing process. Therefore, it is urgent to develop an ideal wound dressing with good biocompatibility and strong antibacterial activity to promote wound healing. Bacterial cellulose is a kind of promising biopolymer because it can control wound exudate and provide a moist environment for wound healing. However, the lack of antibacterial activity limits its application. In this paper, the advantages of bacterial cellulose as wound dressings were introduced, and the preparation and research progress of bacterial cellulose-based antibacterial composites in recent years were reviewed, including adding antibiotics, combining with inorganic antibacterial agents or organic antibacterial agents. Finally, the existing problems and future development direction of bacterial cellulose-based antibacterial wound dressings were discussed.

Brominated flame retardants in home dust and its contribution to brominated flame retardants bioaccumulation in children hair.

Brominated flame retardants (BFRs) in house dust have raised significant concern around the world. However, few studies have reported the correlation between BFR concentrations in house dust and children's hair samples. In this study, BFR concentrations in house dust and children's hair were measured. Chemical analysis showed that the total concentrations of polybrominated diphenyl ethers (PBDEs) in house dust ranged from 334 to 4444 ng g-1, with a median of 442 ng g-1, and the concentrations in children's hair ranged from 352 to 655 ng g-1, with a median of 530 ng g-1. In addition, two alternative flame retardants, pentabromoethylbenzene (PBEB) and hexabromobenzene, were frequently detected in house dust and human hair. BDE209 was the most abundant PBDE congener detected in both house dust and children's hair. A significant correlation was found between the integrated PCA score of BFR concentrations in house dust and in children's hair (r2 = 0.31, P < 0.05), indicating the great contribution of house dust to the bodily burden of PBDEs in children. Risk assessment indicated that children's exposure to PBDEs via non-dietary intake of house dust should be recognized as an important exposure pathway.

Honeycomb-like structure-tunable chitosan-based porous carbon microspheres for methylene blue efficient removal.

Chitosan (CS) can be used for the preparation of carbon materials with different morphologies due to its excellent properties, but there are no reports on its spherical morphology. In this study, a feasible step-by-step strategy was proposed to fabricate nitrogen-containing chitosan-based porous carbon microspheres (CPCM) in HCl and KOH. The unique spherical morphology and honeycomb-like porous structure of CPCM were accurately regulated. A great quantity of micro/mesopores endowed CPCM an ultra-high specific surface area up to 2463.9 m2 g-1. Moreover, CPCM exhibited an ultra-high maximum adsorption capacity up to 1599.03 mg g-1 for methylene blue (MB), meanwhile the adsorption process was in well agreement with the Langmuir isotherm and pseudo-second-order kinetic models. It was simultaneously a favorable reusable adsorbent with high regenerative capacity. The high dye adsorption properties suggest that chitosan can be a promising candidate for sewage treatment in the form of carbon microspheres.

Percutaneous Penetration and Metabolism of Plasticizers by Skin Cells and Its Implication in Dermal Exposure to Plasticizers by Skin Wipes.

Numerous studies focused on the human exposure to plasticizers via dermal contact; however, the percutaneous penetration of plasticizers was seldom considered in exposure assessment. In the present study, skin wipes of palms, back-of-hands, and forehead were collected from 114 participants (ages: 18-27). There was no significant difference between the levels of phthalates from palms and back-of-hand, while all phthalates collected from the forehead were significantly higher than those from palms and back-of-hand (p < 0.001); di(2-ethylhexyl)phthalate levels were substantially higher than other detected phthalates followed by di(n-butyl)phthalate and di(isobutyl)phthalate (DiBP), and for alternative plasticizers, bis-2-ethylhexyl terephthalate levels were substantially higher than acetyltributyl citrate and bis-2-ethylhexyladipate. Skin permeation and metabolism of phthalates was assessed using human skin equivalent models. The permeability coefficient (kp) values of phthalates were significantly negatively correlated with their log octanol-water partition coefficient (log Kow), while a significantly positive correlation was found between the log Kow and the cumulative amounts of phthalates in the cells. The proportion of phthalate intake via dermal exposure to skin wipes ranges from 1.3% (for dimethyl phthalate) to 8.6% (for DiBP) and suggests that dermal absorption is a significant route for adult phthalate exposure.

The comparison of transcriptomic response of green microalga Chlorella sorokiniana exposure to environmentally relevant concentration of cadmium(II) and 4-n-nonylphenol.

The transcriptomic response of green microalga Chlorella sorokiniana exposure to environmentally relevant concentration of cadmium(II) (Cd) and 4-n-nonylphenol (4-n-NP) was compared in the present study. Cd and 4-n-NP exposure showed a similar pattern of dys-regulated pathways. The photosystem was affected due to suppression of chlorophyll biosynthesis via down-regulation of Mg-protoporphyrin IX chelatase subunit ChlD (CHLD) and divinyl chlorophyllide a 8-vinyl-reductase (DVR) in Cd group and via down-regulation of DVR in 4-n-NP group. Furthermore, the reactive oxygen species (ROS) could be induced through down-regulation of solanesyl diphosphate synthase 1 (SPS1) and homogentisate phytyltransferase (HPT) in Cd group and via down-regulation of HPT in 4-n-NP group. Additionally, Cd and 4-n-NP would both cause the dys-regulation of carbohydrate metabolism and protein synthesis. On the other hand, there are some different responses or detoxification mechanism of C. sorokiniana to 4-n-NP stress compared to Cd exposure. The increased ROS would cause the DNA damage and protein destruction in Cd exposure group. Simultaneously, the RNA transcription was dys-regulated and a series of changes in gene expressions were observed. This included lipid metabolism, protein modification, and DNA repair, which involved in response of C. sorokiniana to Cd stress or detoxification of Cd. For 4-n-NP exposure, no effect on lipid metabolism and DNA repair was observed. The nucleotide metabolism including pyrimidine metabolism and purine metabolism was significantly up-regulated in the 4-n-NP exposure group, but not in the Cd exposure group. In addition, 4-n-NP would induce the ubiquitin-mediated proteolysis and proteasomal degradation to diminish the misfolded protein caused by ROS and down-regulation of heat shocking protein 40. In sum, the Cd and 4-n-NP could cause the same toxicological effects via the common pathways and possess similar detoxification mechanism. They also showed different responses in nucleotide metabolism, lipid metabolism, and DNA repair.

Novel magnetic polysaccharide/graphene oxide @Fe3O4 gel beads for adsorbing heavy metal ions.

As a kind of potential absorbent, polysaccharide materials are limited due to weak stability, low absorption and recovery rate. Herein, a novel composite adsorbent - the magnetic composite gel beads (CMC/SA/graphene oxide@Fe3O4) were prepared by combining carboxymethyl chitosan (CMC), sodium alginate (SA) with graphene oxide@Fe3O4, and then utilized for the adsorption of Cu2+, Cd2+ and Pb2+ from the wastewater. The physicochemical property of CMC/SA/graphene oxide@Fe3O4 beads were characterized in detail. The CMC/SA/graphene oxide@Fe3O4 magnetic gel beads could separate easily from the wastewater and showed a higher stability due to the addition of graphene oxide@Fe3O4. The adsorption experiments show that the adsorption of Cu2+, Cd2+ and Pb2+ on the magnetic gel bead was well fitted with pseudo-second-order model and Langmuir isotherm model, and the maximum adsorption capacity of Cu2+, Cd2+ and Pb2+ reached 55.96, 86.28 and 189.04 mg/g, respectively. Moreover, the magnetic gel beads had selective adsorption toward Pb2+. In addition, the magnetic gel beads still obtained 90% of the adsorption rates after five cycles, and showed good adsorption efficiency in the simulated real environment. This work proves that the CMC/SA/graphene oxide@Fe3O4 magnetic gel beads as the adsorbents have promising potential in wastewater treatment.

Dermal bioaccessibility of plasticizers in indoor dust and clothing.

Several studies indicate that human exposure to plasticizers via dermal pathway is not negligible, but the dermal bioaccessibility of phthalates and alternative plasticizers from the important environmental matrix including indoor dust and clothing and the importance weight of dermal exposure to those pollutants have been poorly studied. An in vitro physiologically based extraction test was employed to investigate the dermal bioaccessibility of target phthalates and alternative plasticizers from indoor dust and clothing. Temperature, incubation time, sweat/sebum ratio and solid/liquid ratio were selected to study their effects on the bioaccessibility. The bioaccessibility of Diethyl phthalates (DEP), dibutyl phthalate (DBP), bis-2-ethylhexyl phthalate (DEHP), Acetyl tributyl citrate (ATBC), bis-2-ethylhexyladipate (DEHA) and bis-2-ethylhexyl terephthalate (DEHT) in indoor dust were 66.20 ±â€¯1.93%, 94.27 ±â€¯1.31%, 80.37 ±â€¯8.09%, 75.02 ±â€¯2.12%, 94.50 ±â€¯3.42% and 74.09 ±â€¯3.79%, respectively, under the condition of 1:1 sweat/sebum ratio, 1/100 solid/liquid ratio (indoor dust), 1:1 area/area ratio (1:1, clothing) and 90 min incubation time at 36.3 °C which are chosen based on the experimental results and human physical conditions. DBP showed the highest bioaccessibility in all samples. The time course of the plasticizer release was fitted to a first-order one-compartment model. DBP showed the highest release rate (k1) calculated from the model, which was consistent with the bioaccessibility result. Risk assessment indicated that dermal exposure of DBP was an important exposure route, accounting for about 21.58% of total intake, and indoor dust was an important exposure media when considering the dermal bioaccessibility.